P05103 (MTP7_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase PaeR7I
Short name=M.PaeR7I
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase PaeR7I

Gene names

Name:

paeR7IM

Encoded on

Plasmid pMG7

Organism

Pseudomonas aeruginosa

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PaeR7I endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.
Sequence caution	The sequence CAA27025.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Modification methylase PaeR7I		PRO_0000087977

Sequences

	Sequence		Length	Mass (Da)
	P05103 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 3A9A5409A25BA092	FASTA	549	61,161
	10 20 30 40 50 60 MATEGGLEAR GAIFTRSEMV DFILDLAGYT EDQPLHEKRL LEPSFGGGDF LLPIIQRLLS 70 80 90 100 110 120 AWRAARPNGT EVDDLGDAIR AVELHHDTFR STYAAVVALL KREGLSANAA TALADRWLSQ 130 140 150 160 170 180 GDFLLAPLEG QFDFVVGNPP YVRPELIPAP LLAEYRSRYQ TMYDRADIYI PFIERSLTAL 190 200 210 220 230 240 SAGGNLGFIC ADRWMKNRYG GPLRSLVAER FHLKVYVDMV DTPAFHSDVI AYPAITIISR 250 260 270 280 290 300 EGGGATRIAH RPSIDRATLT TLAGLLSAPT LPKDAGPVRE LARVTNGAER GCWSLLTRWR 310 320 330 340 350 360 LFAVWRARSH CSKRLGARFG IGVATGADKA FIGDFESLDV EPDRKLPLVT TKDIMTGEVQ 370 380 390 400 410 420 WRGQGVINPF AESGGLVDLG EYPRLRRYLE ARRDVIAGRH CAKKAPANWY RTIDRITPAL 430 440 450 460 470 480 AARPKLLIPD IKGESHIVFE GGELYPSHNL YYVTSDDWDL RALQAVLLSA VSRLFVATYS 490 500 510 520 530 540 TKMRGGFLRF QAQYLRRIRI PRWADVPEPL RRELAEAAIK RDVQACNRAV FRLYGLSHEE RSALGGNGE

References

[1]	"Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products." Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R. Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence motifs characteristic for DNA [cytosine-N4] and DNA [adenine-N6] methyltransferases. Classification of all DNA methyltransferases." Timinskas A., Butkus V., Janulaitis A. Gene 157:3-11(1995) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO N-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03274 Genomic DNA. Translation: CAA27025.1. Different initiation.
PIR	XYPS7A. S07366.
3D structure databases
ProteinModelPortal	P05103.
ModBase	Search...
Protein family/group databases
REBASE	3477. M.PaeR7I.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. IPR011639. RM_methylase_Eco57I. IPR025931. TaqI_C. [Graphical view]
Pfam	PF07669. Eco57I. 1 hit. PF12950. TaqI_C. 1 hit. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTP7_PSEAI

Accession

Primary (citable) accession number: P05103

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program