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P05103 (MTP7_PSEAI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase PaeR7I

Short name=M.PaeR7I
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase PaeR7I
Gene names
Name:paeR7IM
Encoded onPlasmid pMG7
OrganismPseudomonas aeruginosa
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PaeR7I endonuclease. Ref.1

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Monomer. Ref.1

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence caution

The sequence CAA27025.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence CAA27025.1 differs from that shown. Reason: Frameshift at positions 315 and 344.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

site-specific DNA-methyltransferase (adenine-specific) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 574574Modification methylase PaeR7I
PRO_0000087977

Sequences

Sequence LengthMass (Da)Tools
P05103 [UniParc].

Last modified February 19, 2014. Version 3.
Checksum: 31ABC8948DD1EFAD

FASTA57463,229
        10         20         30         40         50         60 
MAFAPSVAHK PVAAAVCPVM AATEALATEG GLEARGAIFT RSEVVDFILD LAGYTEDQPL 

        70         80         90        100        110        120 
HEKRLLEPSF GGGDFLLPII QRLLSAWRAA RPNGTEVDDL GDAIRAVELH HDTFRSTYAA 

       130        140        150        160        170        180 
VVALLKREGL SANAATALAD RWLSQGDFLL APLEGQFDFV VGNPPYVRPE LIPAPLLAEY 

       190        200        210        220        230        240 
RSRYQTMYDR ADIYIPFIER SLTALSAGGN LGFICADRWM KNRYGGPLRS LVAERFHLKV 

       250        260        270        280        290        300 
YVDMVDTPAF HSDVIAYPAI TIISREGGGA TRIAHRPSID RATLTTLAGL LSAPTLPKDA 

       310        320        330        340        350        360 
GPVRELARVT NGAEPWLLES SDQMALIRRL EGAFPLLEEA GCKVGIGVAT GADKAFIGDF 

       370        380        390        400        410        420 
ESLDVEPDRK LPLVTTKDIM TGEVQWRGQG VINPFAESGG LVDLGEYPRL RRYLEARRDV 

       430        440        450        460        470        480 
IAGRHCAKKA PANWYRTIDR ITPALAARPK LLIPDIKGES HIVFEGGELY PSHNLYYVTS 

       490        500        510        520        530        540 
DDWDLRALQA VLLSAVSRLF VATYSTKMRG GFLRFQAQYL RRIRIPRWAD VPEPLRRELA 

       550        560        570 
EAAIKRDVQA CNRAVFRLYG LSHEERSALG GNGE 

« Hide

References

[1]"Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products."
Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R.
Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A MODIFICATION METHYLASE, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03274 Genomic DNA. Translation: CAA27025.1. Sequence problems.
PIRXYPS7A. S07366.

3D structure databases

ProteinModelPortalP05103.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3477. M.PaeR7I.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR025931. TaqI_C.
[Graphical view]
PfamPF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTP7_PSEAI
AccessionPrimary (citable) accession number: P05103
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 19, 2014
Last modified: April 16, 2014
This is version 77 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries