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P05103

- MTP7_PSEAI

UniProt

P05103 - MTP7_PSEAI

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Protein
Modification methylase PaeR7I
Gene
paeR7IM
Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PaeR7I endonuclease.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3477. M.PaeR7I.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase PaeR7I (EC:2.1.1.72)
Short name:
M.PaeR7I
Alternative name(s):
Adenine-specific methyltransferase PaeR7I
Gene namesi
Name:paeR7IM
Encoded oniPlasmid pMG70 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Modification methylase PaeR7I
PRO_0000087977Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05103-1 [UniParc]FASTAAdd to Basket

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MAFAPSVAHK PVAAAVCPVM AATEALATEG GLEARGAIFT RSEVVDFILD    50
LAGYTEDQPL HEKRLLEPSF GGGDFLLPII QRLLSAWRAA RPNGTEVDDL 100
GDAIRAVELH HDTFRSTYAA VVALLKREGL SANAATALAD RWLSQGDFLL 150
APLEGQFDFV VGNPPYVRPE LIPAPLLAEY RSRYQTMYDR ADIYIPFIER 200
SLTALSAGGN LGFICADRWM KNRYGGPLRS LVAERFHLKV YVDMVDTPAF 250
HSDVIAYPAI TIISREGGGA TRIAHRPSID RATLTTLAGL LSAPTLPKDA 300
GPVRELARVT NGAEPWLLES SDQMALIRRL EGAFPLLEEA GCKVGIGVAT 350
GADKAFIGDF ESLDVEPDRK LPLVTTKDIM TGEVQWRGQG VINPFAESGG 400
LVDLGEYPRL RRYLEARRDV IAGRHCAKKA PANWYRTIDR ITPALAARPK 450
LLIPDIKGES HIVFEGGELY PSHNLYYVTS DDWDLRALQA VLLSAVSRLF 500
VATYSTKMRG GFLRFQAQYL RRIRIPRWAD VPEPLRRELA EAAIKRDVQA 550
CNRAVFRLYG LSHEERSALG GNGE 574
Length:574
Mass (Da):63,229
Last modified:February 19, 2014 - v3
Checksum:i31ABC8948DD1EFAD
GO

Sequence cautioni

The sequence CAA27025.1 differs from that shown. Reason: Frameshift at positions 315 and 344.
The sequence CAA27025.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03274 Genomic DNA. Translation: CAA27025.1. Sequence problems.
PIRiS07366. XYPS7A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03274 Genomic DNA. Translation: CAA27025.1 . Sequence problems.
PIRi S07366. XYPS7A.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3477. M.PaeR7I.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases-like.
IPR025931. TaqI_C.
[Graphical view ]
Pfami PF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view ]
PRINTSi PR00507. N12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products."
    Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R.
    Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A MODIFICATION METHYLASE, SUBUNIT.

Entry informationi

Entry nameiMTP7_PSEAI
AccessioniPrimary (citable) accession number: P05103
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 19, 2014
Last modified: June 11, 2014
This is version 78 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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