Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Modification methylase PaeR7I

Gene

paeR7IM

Organism
Pseudomonas aeruginosa
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PaeR7I endonuclease.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3477 M.PaeR7I

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase PaeR7I (EC:2.1.1.72)
Short name:
M.PaeR7I
Alternative name(s):
Adenine-specific methyltransferase PaeR7I
Gene namesi
Name:paeR7IM
Encoded oniPlasmid pMG70 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879771 – 574Modification methylase PaeR7IAdd BLAST574

Proteomic databases

PRIDEiP05103

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

InterProiView protein in InterPro
IPR002052 DNA_methylase_N6_adenine_CS
IPR011639 RM_methylase_Eco57I
IPR029063 SAM-dependent_MTases
IPR025931 TaqI_C
PfamiView protein in Pfam
PF07669 Eco57I, 1 hit
PF12950 TaqI_C, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS00092 N6_MTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P05103-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFAPSVAHK PVAAAVCPVM AATEALATEG GLEARGAIFT RSEVVDFILD
60 70 80 90 100
LAGYTEDQPL HEKRLLEPSF GGGDFLLPII QRLLSAWRAA RPNGTEVDDL
110 120 130 140 150
GDAIRAVELH HDTFRSTYAA VVALLKREGL SANAATALAD RWLSQGDFLL
160 170 180 190 200
APLEGQFDFV VGNPPYVRPE LIPAPLLAEY RSRYQTMYDR ADIYIPFIER
210 220 230 240 250
SLTALSAGGN LGFICADRWM KNRYGGPLRS LVAERFHLKV YVDMVDTPAF
260 270 280 290 300
HSDVIAYPAI TIISREGGGA TRIAHRPSID RATLTTLAGL LSAPTLPKDA
310 320 330 340 350
GPVRELARVT NGAEPWLLES SDQMALIRRL EGAFPLLEEA GCKVGIGVAT
360 370 380 390 400
GADKAFIGDF ESLDVEPDRK LPLVTTKDIM TGEVQWRGQG VINPFAESGG
410 420 430 440 450
LVDLGEYPRL RRYLEARRDV IAGRHCAKKA PANWYRTIDR ITPALAARPK
460 470 480 490 500
LLIPDIKGES HIVFEGGELY PSHNLYYVTS DDWDLRALQA VLLSAVSRLF
510 520 530 540 550
VATYSTKMRG GFLRFQAQYL RRIRIPRWAD VPEPLRRELA EAAIKRDVQA
560 570
CNRAVFRLYG LSHEERSALG GNGE
Length:574
Mass (Da):63,229
Last modified:February 19, 2014 - v3
Checksum:i31ABC8948DD1EFAD
GO

Sequence cautioni

The sequence CAA27025 differs from that shown. Reason: Frameshift at positions 315 and 344.Curated
The sequence CAA27025 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03274 Genomic DNA Translation: CAA27025.1 Sequence problems.
PIRiS07366 XYPS7A

Similar proteinsi

Entry informationi

Entry nameiMTP7_PSEAI
AccessioniPrimary (citable) accession number: P05103
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 19, 2014
Last modified: May 23, 2018
This is version 88 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health