Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Modification methylase PaeR7I

Gene

paeR7IM

Organism
Pseudomonas aeruginosa
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PaeR7I endonuclease.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3477. M.PaeR7I.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase PaeR7I (EC:2.1.1.72)
Short name:
M.PaeR7I
Alternative name(s):
Adenine-specific methyltransferase PaeR7I
Gene namesi
Name:paeR7IM
Encoded oniPlasmid pMG70 Publication
OrganismiPseudomonas aeruginosa
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Modification methylase PaeR7IPRO_0000087977Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05103-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFAPSVAHK PVAAAVCPVM AATEALATEG GLEARGAIFT RSEVVDFILD
60 70 80 90 100
LAGYTEDQPL HEKRLLEPSF GGGDFLLPII QRLLSAWRAA RPNGTEVDDL
110 120 130 140 150
GDAIRAVELH HDTFRSTYAA VVALLKREGL SANAATALAD RWLSQGDFLL
160 170 180 190 200
APLEGQFDFV VGNPPYVRPE LIPAPLLAEY RSRYQTMYDR ADIYIPFIER
210 220 230 240 250
SLTALSAGGN LGFICADRWM KNRYGGPLRS LVAERFHLKV YVDMVDTPAF
260 270 280 290 300
HSDVIAYPAI TIISREGGGA TRIAHRPSID RATLTTLAGL LSAPTLPKDA
310 320 330 340 350
GPVRELARVT NGAEPWLLES SDQMALIRRL EGAFPLLEEA GCKVGIGVAT
360 370 380 390 400
GADKAFIGDF ESLDVEPDRK LPLVTTKDIM TGEVQWRGQG VINPFAESGG
410 420 430 440 450
LVDLGEYPRL RRYLEARRDV IAGRHCAKKA PANWYRTIDR ITPALAARPK
460 470 480 490 500
LLIPDIKGES HIVFEGGELY PSHNLYYVTS DDWDLRALQA VLLSAVSRLF
510 520 530 540 550
VATYSTKMRG GFLRFQAQYL RRIRIPRWAD VPEPLRRELA EAAIKRDVQA
560 570
CNRAVFRLYG LSHEERSALG GNGE
Length:574
Mass (Da):63,229
Last modified:February 19, 2014 - v3
Checksum:i31ABC8948DD1EFAD
GO

Sequence cautioni

The sequence CAA27025.1 differs from that shown. Reason: Frameshift at positions 315 and 344. Curated
The sequence CAA27025.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03274 Genomic DNA. Translation: CAA27025.1. Sequence problems.
PIRiS07366. XYPS7A.
RefSeqiWP_032488422.1. NG_034412.1.
YP_009073969.1. NG_034412.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03274 Genomic DNA. Translation: CAA27025.1. Sequence problems.
PIRiS07366. XYPS7A.
RefSeqiWP_032488422.1. NG_034412.1.
YP_009073969.1. NG_034412.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3477. M.PaeR7I.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR011639. RM_methylase_Eco57I.
IPR029063. SAM-dependent_MTases.
IPR025931. TaqI_C.
[Graphical view]
PfamiPF07669. Eco57I. 1 hit.
PF12950. TaqI_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products."
    Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R.
    Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A MODIFICATION METHYLASE, SUBUNIT.

Entry informationi

Entry nameiMTP7_PSEAI
AccessioniPrimary (citable) accession number: P05103
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: February 19, 2014
Last modified: April 29, 2015
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.