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P05103

- MTP7_PSEAI

UniProt

P05103 - MTP7_PSEAI

Protein

Modification methylase PaeR7I

Gene

paeR7IM

Organism
Pseudomonas aeruginosa
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 3 (19 Feb 2014)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CTCGAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PaeR7I endonuclease.1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3477. M.PaeR7I.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase PaeR7I (EC:2.1.1.72)
    Short name:
    M.PaeR7I
    Alternative name(s):
    Adenine-specific methyltransferase PaeR7I
    Gene namesi
    Name:paeR7IM
    Encoded oniPlasmid pMG70 Publication
    OrganismiPseudomonas aeruginosa
    Taxonomic identifieri287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Modification methylase PaeR7IPRO_0000087977Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view]
    PfamiPF07669. Eco57I. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view]
    PRINTSiPR00507. N12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05103-1 [UniParc]FASTAAdd to Basket

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    MAFAPSVAHK PVAAAVCPVM AATEALATEG GLEARGAIFT RSEVVDFILD    50
    LAGYTEDQPL HEKRLLEPSF GGGDFLLPII QRLLSAWRAA RPNGTEVDDL 100
    GDAIRAVELH HDTFRSTYAA VVALLKREGL SANAATALAD RWLSQGDFLL 150
    APLEGQFDFV VGNPPYVRPE LIPAPLLAEY RSRYQTMYDR ADIYIPFIER 200
    SLTALSAGGN LGFICADRWM KNRYGGPLRS LVAERFHLKV YVDMVDTPAF 250
    HSDVIAYPAI TIISREGGGA TRIAHRPSID RATLTTLAGL LSAPTLPKDA 300
    GPVRELARVT NGAEPWLLES SDQMALIRRL EGAFPLLEEA GCKVGIGVAT 350
    GADKAFIGDF ESLDVEPDRK LPLVTTKDIM TGEVQWRGQG VINPFAESGG 400
    LVDLGEYPRL RRYLEARRDV IAGRHCAKKA PANWYRTIDR ITPALAARPK 450
    LLIPDIKGES HIVFEGGELY PSHNLYYVTS DDWDLRALQA VLLSAVSRLF 500
    VATYSTKMRG GFLRFQAQYL RRIRIPRWAD VPEPLRRELA EAAIKRDVQA 550
    CNRAVFRLYG LSHEERSALG GNGE 574
    Length:574
    Mass (Da):63,229
    Last modified:February 19, 2014 - v3
    Checksum:i31ABC8948DD1EFAD
    GO

    Sequence cautioni

    The sequence CAA27025.1 differs from that shown. Reason: Frameshift at positions 315 and 344.
    The sequence CAA27025.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03274 Genomic DNA. Translation: CAA27025.1. Sequence problems.
    PIRiS07366. XYPS7A.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03274 Genomic DNA. Translation: CAA27025.1 . Sequence problems.
    PIRi S07366. XYPS7A.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3477. M.PaeR7I.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR002296. N12N6_MeTrfase.
    IPR011639. RM_methylase_Eco57I.
    IPR029063. SAM-dependent_MTases-like.
    IPR025931. TaqI_C.
    [Graphical view ]
    Pfami PF07669. Eco57I. 1 hit.
    PF12950. TaqI_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00507. N12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products."
      Theriault G., Roy P.H., Howard K.A., Benner J.S., Brooks J.E., Waters A.F., Gingeras T.R.
      Nucleic Acids Res. 13:8441-8461(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A MODIFICATION METHYLASE, SUBUNIT.

    Entry informationi

    Entry nameiMTP7_PSEAI
    AccessioniPrimary (citable) accession number: P05103
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: February 19, 2014
    Last modified: October 1, 2014
    This is version 79 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3