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P05101

- MTE2_ECOLX

UniProt

P05101 - MTE2_ECOLX

Protein

Modification methylase EcoRII

Gene

ecoRIIM

Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei186 – 1861PROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
    2. DNA binding Source: InterPro

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3396. M.EcoRII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase EcoRII (EC:2.1.1.37)
    Short name:
    M.EcoRII
    Alternative name(s):
    Cytosine-specific methyltransferase EcoRII
    Gene namesi
    Name:ecoRIIM
    Encoded oniPlasmid N30 Publication
    OrganismiEscherichia coli
    Taxonomic identifieri562 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Modification methylase EcoRIIPRO_0000087874Add
    BLAST

    Proteomic databases

    PRIDEiP05101.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP05101.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini96 – 466371SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
    Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10629. PTHR10629. 1 hit.
    PfamiPF00145. DNA_methylase. 1 hit.
    [Graphical view]
    PRINTSiPR00105. C5METTRFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00675. dcm. 1 hit.
    PROSITEiPS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P05101-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEFELLAQD LLEKAEAEEQ LRQENDKKLL GQVLEIYDQK YVAELLRKVG    50
    KNEWSRETLN RWINGKCSPK TLTLAEEELL RKMLPEAPAH HPDYAFRFID 100
    LFAGIGGIRK GFETIGGQCV FTSEWNKEAV RTYKANWFND AQEHTFNLDI 150
    REVTLSDKPE VPENDAYAYI NEHVPDHDVL LAGFPCQPFS LAGVSKKNSL 200
    GRAHGFECEA QGTLFFDVAR IIRAKKPAIF VLENVKNLKS HDKGKTFKVI 250
    MDTLDELGYE VADAAEMGKN DPKVIDGKHF LPQHRERIVL VGFRRDLNIH 300
    QGFTLRDISR FYPEQRPSFG ELLEPVVDSK YILTPKLWEY LYNYAKKHAA 350
    KGNGFGFGLV NPENKESIAR TLSARYHKDG SEILIDRGWD MATGETDFAN 400
    EENQAHRPRR LTPRECARLM GFEKVDGRPF RIPVSDTQSY RQFGNSVVVP 450
    VFEAVAKLLE PYILKAVNAD SCKVERI 477
    Length:477
    Mass (Da):54,556
    Last modified:August 13, 1987 - v1
    Checksum:i9E595D3809BCCAEA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05050 Genomic DNA. Translation: CAA28725.1.
    PIRiA25871. XYECR2.
    RefSeqiYP_007349454.1. NC_020086.1.
    YP_008574830.1. NC_022374.1.
    YP_008574963.1. NC_022375.1.
    YP_009023119.1. NC_023909.1.
    YP_009023200.1. NC_023910.1.
    YP_724464.1. NC_007682.3.

    Genome annotation databases

    GeneIDi15230097.
    17035703.
    17047048.
    18983620.
    18983702.
    4246757.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X05050 Genomic DNA. Translation: CAA28725.1 .
    PIRi A25871. XYECR2.
    RefSeqi YP_007349454.1. NC_020086.1.
    YP_008574830.1. NC_022374.1.
    YP_008574963.1. NC_022375.1.
    YP_009023119.1. NC_023909.1.
    YP_009023200.1. NC_023910.1.
    YP_724464.1. NC_007682.3.

    3D structure databases

    ProteinModelPortali P05101.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3396. M.EcoRII.

    Proteomic databases

    PRIDEi P05101.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 15230097.
    17035703.
    17047048.
    18983620.
    18983702.
    4246757.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR018117. C5_DNA_meth_AS.
    IPR001525. C5_MeTfrase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10629. PTHR10629. 1 hit.
    Pfami PF00145. DNA_methylase. 1 hit.
    [Graphical view ]
    PRINTSi PR00105. C5METTRFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00675. dcm. 1 hit.
    PROSITEi PS00094. C5_MTASE_1. 1 hit.
    PS00095. C5_MTASE_2. 1 hit.
    PS51679. SAM_MT_C5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and expression of the gene encoding the EcoRII modification enzyme."
      Som S., Bhagwat A.S., Friedman S.
      Nucleic Acids Res. 15:313-332(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 33694 / HB101.

    Entry informationi

    Entry nameiMTE2_ECOLX
    AccessioniPrimary (citable) accession number: P05101
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Plasmid

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3