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Protein

Modification methylase EcoRII

Gene

ecoRIIM

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei186 – 1861PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3396. M.EcoRII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcoRII (EC:2.1.1.37)
Short name:
M.EcoRII
Alternative name(s):
Cytosine-specific methyltransferase EcoRII
Gene namesi
Name:ecoRIIM
Encoded oniPlasmid N30 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Modification methylase EcoRIIPRO_0000087874Add
BLAST

Proteomic databases

PRIDEiP05101.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP05101.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 466371SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK00558.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFELLAQD LLEKAEAEEQ LRQENDKKLL GQVLEIYDQK YVAELLRKVG
60 70 80 90 100
KNEWSRETLN RWINGKCSPK TLTLAEEELL RKMLPEAPAH HPDYAFRFID
110 120 130 140 150
LFAGIGGIRK GFETIGGQCV FTSEWNKEAV RTYKANWFND AQEHTFNLDI
160 170 180 190 200
REVTLSDKPE VPENDAYAYI NEHVPDHDVL LAGFPCQPFS LAGVSKKNSL
210 220 230 240 250
GRAHGFECEA QGTLFFDVAR IIRAKKPAIF VLENVKNLKS HDKGKTFKVI
260 270 280 290 300
MDTLDELGYE VADAAEMGKN DPKVIDGKHF LPQHRERIVL VGFRRDLNIH
310 320 330 340 350
QGFTLRDISR FYPEQRPSFG ELLEPVVDSK YILTPKLWEY LYNYAKKHAA
360 370 380 390 400
KGNGFGFGLV NPENKESIAR TLSARYHKDG SEILIDRGWD MATGETDFAN
410 420 430 440 450
EENQAHRPRR LTPRECARLM GFEKVDGRPF RIPVSDTQSY RQFGNSVVVP
460 470
VFEAVAKLLE PYILKAVNAD SCKVERI
Length:477
Mass (Da):54,556
Last modified:August 13, 1987 - v1
Checksum:i9E595D3809BCCAEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05050 Genomic DNA. Translation: CAA28725.1.
PIRiA25871. XYECR2.
RefSeqiWP_001288432.1. NZ_LAXC01000071.1.
YP_007349454.1. NC_020086.1.
YP_008574830.1. NC_022374.1.
YP_008574963.1. NC_022375.1.
YP_009023119.1. NC_023909.1.
YP_009023200.1. NC_023910.1.
YP_009060562.1. NC_024967.1.
YP_009068675.1. NC_025141.1.
YP_009071472.1. NC_025183.1.
YP_724464.1. NC_007682.3.

Genome annotation databases

GeneIDi15230097.
17035703.
17047048.
18983620.
18983702.
20467080.
20491807.
20493544.
23339583.
4246757.
KEGGipg:15230097.
pg:17035703.
pg:17047048.
pg:18983620.
pg:18983702.
pg:20467080.
pg:20491807.
pg:20493544.
pg:4246757.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05050 Genomic DNA. Translation: CAA28725.1.
PIRiA25871. XYECR2.
RefSeqiWP_001288432.1. NZ_LAXC01000071.1.
YP_007349454.1. NC_020086.1.
YP_008574830.1. NC_022374.1.
YP_008574963.1. NC_022375.1.
YP_009023119.1. NC_023909.1.
YP_009023200.1. NC_023910.1.
YP_009060562.1. NC_024967.1.
YP_009068675.1. NC_025141.1.
YP_009071472.1. NC_025183.1.
YP_724464.1. NC_007682.3.

3D structure databases

ProteinModelPortaliP05101.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3396. M.EcoRII.

Proteomic databases

PRIDEiP05101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi15230097.
17035703.
17047048.
18983620.
18983702.
20467080.
20491807.
20493544.
23339583.
4246757.
KEGGipg:15230097.
pg:17035703.
pg:17047048.
pg:18983620.
pg:18983702.
pg:20467080.
pg:20491807.
pg:20493544.
pg:4246757.

Phylogenomic databases

KOiK00558.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence and expression of the gene encoding the EcoRII modification enzyme."
    Som S., Bhagwat A.S., Friedman S.
    Nucleic Acids Res. 15:313-332(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 33694 / HB101.

Entry informationi

Entry nameiMTE2_ECOLX
AccessioniPrimary (citable) accession number: P05101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 22, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.