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Protein

Modification methylase EcoRII

Gene

ecoRIIM

Organism
Escherichia coli
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Protein family/group databases

REBASEi3396 M.EcoRII

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcoRII (EC:2.1.1.37)
Short name:
M.EcoRII
Alternative name(s):
Cytosine-specific methyltransferase EcoRII
Gene namesi
Name:ecoRIIM
Encoded oniPlasmid N30 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000878741 – 477Modification methylase EcoRIIAdd BLAST477

Proteomic databases

PRIDEiP05101

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP05101
SMRiP05101
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 466SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST371

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P05101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEFELLAQD LLEKAEAEEQ LRQENDKKLL GQVLEIYDQK YVAELLRKVG
60 70 80 90 100
KNEWSRETLN RWINGKCSPK TLTLAEEELL RKMLPEAPAH HPDYAFRFID
110 120 130 140 150
LFAGIGGIRK GFETIGGQCV FTSEWNKEAV RTYKANWFND AQEHTFNLDI
160 170 180 190 200
REVTLSDKPE VPENDAYAYI NEHVPDHDVL LAGFPCQPFS LAGVSKKNSL
210 220 230 240 250
GRAHGFECEA QGTLFFDVAR IIRAKKPAIF VLENVKNLKS HDKGKTFKVI
260 270 280 290 300
MDTLDELGYE VADAAEMGKN DPKVIDGKHF LPQHRERIVL VGFRRDLNIH
310 320 330 340 350
QGFTLRDISR FYPEQRPSFG ELLEPVVDSK YILTPKLWEY LYNYAKKHAA
360 370 380 390 400
KGNGFGFGLV NPENKESIAR TLSARYHKDG SEILIDRGWD MATGETDFAN
410 420 430 440 450
EENQAHRPRR LTPRECARLM GFEKVDGRPF RIPVSDTQSY RQFGNSVVVP
460 470
VFEAVAKLLE PYILKAVNAD SCKVERI
Length:477
Mass (Da):54,556
Last modified:August 13, 1987 - v1
Checksum:i9E595D3809BCCAEA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05050 Genomic DNA Translation: CAA28725.1
PIRiA25871 XYECR2
RefSeqiWP_001288432.1, NZ_PCFN01000018.1
YP_007349454.1, NC_020086.1
YP_008574830.1, NC_022374.1
YP_008574963.1, NC_022375.1
YP_009023119.1, NC_023909.1
YP_009023200.1, NC_023910.1
YP_009060562.1, NC_024967.1
YP_009068675.1, NC_025141.1
YP_009071472.1, NC_025183.1
YP_724464.1, NC_007682.3

Genome annotation databases

GeneIDi15230097
17035703
17047048
18983620
18983702
20467080
20491807
20493544
23339583
4246757

Similar proteinsi

Entry informationi

Entry nameiMTE2_ECOLX
AccessioniPrimary (citable) accession number: P05101
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid
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Main funding by: National Institutes of Health