P05101 (MTE2_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase EcoRII
Short name=M.EcoRII
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase EcoRII

Gene names

Name:

ecoRIIM

Encoded on

Plasmid N3

Organism

Escherichia coli

Taxonomic identifier

562 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Subunit structure	Monomer.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Modification methylase EcoRII		PRO_0000087874
Sites
	Active site	186	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P05101 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 9E595D3809BCCAEA	FASTA	477	54,556
	10 20 30 40 50 60 MSEFELLAQD LLEKAEAEEQ LRQENDKKLL GQVLEIYDQK YVAELLRKVG KNEWSRETLN 70 80 90 100 110 120 RWINGKCSPK TLTLAEEELL RKMLPEAPAH HPDYAFRFID LFAGIGGIRK GFETIGGQCV 130 140 150 160 170 180 FTSEWNKEAV RTYKANWFND AQEHTFNLDI REVTLSDKPE VPENDAYAYI NEHVPDHDVL 190 200 210 220 230 240 LAGFPCQPFS LAGVSKKNSL GRAHGFECEA QGTLFFDVAR IIRAKKPAIF VLENVKNLKS 250 260 270 280 290 300 HDKGKTFKVI MDTLDELGYE VADAAEMGKN DPKVIDGKHF LPQHRERIVL VGFRRDLNIH 310 320 330 340 350 360 QGFTLRDISR FYPEQRPSFG ELLEPVVDSK YILTPKLWEY LYNYAKKHAA KGNGFGFGLV 370 380 390 400 410 420 NPENKESIAR TLSARYHKDG SEILIDRGWD MATGETDFAN EENQAHRPRR LTPRECARLM 430 440 450 460 470 GFEKVDGRPF RIPVSDTQSY RQFGNSVVVP VFEAVAKLLE PYILKAVNAD SCKVERI

References

[1]	"Nucleotide sequence and expression of the gene encoding the EcoRII modification enzyme." Som S., Bhagwat A.S., Friedman S. Nucleic Acids Res. 15:313-332(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33694 / HB101.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X05050 Genomic DNA. Translation: CAA28725.1.
PIR	XYECR2. A25871.
RefSeq	YP_724464.1. NC_007682.3.
3D structure databases
ProteinModelPortal	P05101.
ModBase	Search...
Protein family/group databases
REBASE	3396. M.EcoRII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4246757.
Phylogenomic databases
ProtClustDB	PRK10458.
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTE2_ECOLX

Accession

Primary (citable) accession number: P05101

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program