Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P05101 (MTE2_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase EcoRII

Short name=M.EcoRII
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase EcoRII
Gene names
Name:ecoRIIM
Encoded onPlasmid N3
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Subunit structure

Monomer.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Modification methylase EcoRII
PRO_0000087874

Regions

Domain96 – 466371SAM-dependent MTase C5-type

Sites

Active site1861 By similarity

Sequences

Sequence LengthMass (Da)Tools
P05101 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 9E595D3809BCCAEA

FASTA47754,556
        10         20         30         40         50         60 
MSEFELLAQD LLEKAEAEEQ LRQENDKKLL GQVLEIYDQK YVAELLRKVG KNEWSRETLN 

        70         80         90        100        110        120 
RWINGKCSPK TLTLAEEELL RKMLPEAPAH HPDYAFRFID LFAGIGGIRK GFETIGGQCV 

       130        140        150        160        170        180 
FTSEWNKEAV RTYKANWFND AQEHTFNLDI REVTLSDKPE VPENDAYAYI NEHVPDHDVL 

       190        200        210        220        230        240 
LAGFPCQPFS LAGVSKKNSL GRAHGFECEA QGTLFFDVAR IIRAKKPAIF VLENVKNLKS 

       250        260        270        280        290        300 
HDKGKTFKVI MDTLDELGYE VADAAEMGKN DPKVIDGKHF LPQHRERIVL VGFRRDLNIH 

       310        320        330        340        350        360 
QGFTLRDISR FYPEQRPSFG ELLEPVVDSK YILTPKLWEY LYNYAKKHAA KGNGFGFGLV 

       370        380        390        400        410        420 
NPENKESIAR TLSARYHKDG SEILIDRGWD MATGETDFAN EENQAHRPRR LTPRECARLM 

       430        440        450        460        470 
GFEKVDGRPF RIPVSDTQSY RQFGNSVVVP VFEAVAKLLE PYILKAVNAD SCKVERI 

« Hide

References

[1]"Nucleotide sequence and expression of the gene encoding the EcoRII modification enzyme."
Som S., Bhagwat A.S., Friedman S.
Nucleic Acids Res. 15:313-332(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 33694 / HB101.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X05050 Genomic DNA. Translation: CAA28725.1.
PIRXYECR2. A25871.
RefSeqYP_007349454.1. NC_020086.1.
YP_008574830.1. NC_022374.1.
YP_008574963.1. NC_022375.1.
YP_724464.1. NC_007682.3.

3D structure databases

ProteinModelPortalP05101.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3396. M.EcoRII.

Proteomic databases

PRIDEP05101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID15230097.
17035703.
17047048.
4246757.

Phylogenomic databases

ProtClustDBPRK10458.

Family and domain databases

InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTE2_ECOLX
AccessionPrimary (citable) accession number: P05101
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries