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P05101

- MTE2_ECOLX

UniProt

P05101 - MTE2_ECOLX

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Protein

Modification methylase EcoRII

Gene
ecoRIIM
Organism
Escherichia coli
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the EcoRII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei186 – 1861 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3396. M.EcoRII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase EcoRII (EC:2.1.1.37)
Short name:
M.EcoRII
Alternative name(s):
Cytosine-specific methyltransferase EcoRII
Gene namesi
Name:ecoRIIM
Encoded oniPlasmid N30 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Modification methylase EcoRIIPRO_0000087874Add
BLAST

Proteomic databases

PRIDEiP05101.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP05101.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 466371SAM-dependent MTase C5-typeAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05101-1 [UniParc]FASTAAdd to Basket

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MSEFELLAQD LLEKAEAEEQ LRQENDKKLL GQVLEIYDQK YVAELLRKVG    50
KNEWSRETLN RWINGKCSPK TLTLAEEELL RKMLPEAPAH HPDYAFRFID 100
LFAGIGGIRK GFETIGGQCV FTSEWNKEAV RTYKANWFND AQEHTFNLDI 150
REVTLSDKPE VPENDAYAYI NEHVPDHDVL LAGFPCQPFS LAGVSKKNSL 200
GRAHGFECEA QGTLFFDVAR IIRAKKPAIF VLENVKNLKS HDKGKTFKVI 250
MDTLDELGYE VADAAEMGKN DPKVIDGKHF LPQHRERIVL VGFRRDLNIH 300
QGFTLRDISR FYPEQRPSFG ELLEPVVDSK YILTPKLWEY LYNYAKKHAA 350
KGNGFGFGLV NPENKESIAR TLSARYHKDG SEILIDRGWD MATGETDFAN 400
EENQAHRPRR LTPRECARLM GFEKVDGRPF RIPVSDTQSY RQFGNSVVVP 450
VFEAVAKLLE PYILKAVNAD SCKVERI 477
Length:477
Mass (Da):54,556
Last modified:August 13, 1987 - v1
Checksum:i9E595D3809BCCAEA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05050 Genomic DNA. Translation: CAA28725.1.
PIRiA25871. XYECR2.
RefSeqiYP_007349454.1. NC_020086.1.
YP_008574830.1. NC_022374.1.
YP_008574963.1. NC_022375.1.
YP_009023119.1. NC_023909.1.
YP_009023200.1. NC_023910.1.
YP_724464.1. NC_007682.3.

Genome annotation databases

GeneIDi15230097.
17035703.
17047048.
18983620.
18983702.
4246757.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X05050 Genomic DNA. Translation: CAA28725.1 .
PIRi A25871. XYECR2.
RefSeqi YP_007349454.1. NC_020086.1.
YP_008574830.1. NC_022374.1.
YP_008574963.1. NC_022375.1.
YP_009023119.1. NC_023909.1.
YP_009023200.1. NC_023910.1.
YP_724464.1. NC_007682.3.

3D structure databases

ProteinModelPortali P05101.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3396. M.EcoRII.

Proteomic databases

PRIDEi P05101.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 15230097.
17035703.
17047048.
18983620.
18983702.
4246757.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and expression of the gene encoding the EcoRII modification enzyme."
    Som S., Bhagwat A.S., Friedman S.
    Nucleic Acids Res. 15:313-332(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 33694 / HB101.

Entry informationi

Entry nameiMTE2_ECOLX
AccessioniPrimary (citable) accession number: P05101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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