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Protein

DNA-3-methyladenine glycosylase 1

Gene

tag

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions.1 Publication

Catalytic activityi

Hydrolysis of alkylated DNA, releasing 3-methyladenine.1 Publication

Enzyme regulationi

Activity is controlled by product inhibition.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi4 – 41ZincCombined sources2 Publications
Metal bindingi17 – 171Zinc; via tele nitrogenCombined sources2 Publications
Metal bindingi175 – 1751Zinc; via pros nitrogenCombined sources2 Publications
Metal bindingi179 – 1791ZincCombined sources2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10986-MONOMER.
ECOL316407:JW3518-MONOMER.
MetaCyc:EG10986-MONOMER.
RETL1328306-WGS:GSTH-825-MONOMER.
BRENDAi3.2.2.20. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-3-methyladenine glycosylase 1 (EC:3.2.2.201 Publication)
Alternative name(s):
3-methyladenine-DNA glycosylase I, constitutive
Short name:
TAG I
DNA-3-methyladenine glycosidase I
DNA-3-methyladenine glycosylase I
Gene namesi
Name:tag2 Publications
Ordered Locus Names:b3549, JW3518
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10986. tag.

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2295563.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187DNA-3-methyladenine glycosylase 1PRO_0000194877Add
BLAST

Proteomic databases

EPDiP05100.
PaxDbiP05100.

Interactioni

Protein-protein interaction databases

BioGridi4261555. 184 interactions.
DIPiDIP-10950N.
IntActiP05100. 5 interactions.
MINTiMINT-1230071.
STRINGi511145.b3549.

Structurei

Secondary structure

1
187
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni5 – 95Combined sources
Helixi12 – 198Combined sources
Beta strandi20 – 234Combined sources
Helixi28 – 3912Combined sources
Turni40 – 434Combined sources
Helixi46 – 7025Combined sources
Helixi73 – 819Combined sources
Beta strandi82 – 854Combined sources
Helixi90 – 10718Combined sources
Helixi112 – 1187Combined sources
Turni119 – 1224Combined sources
Turni132 – 1343Combined sources
Helixi140 – 15213Combined sources
Helixi159 – 16911Combined sources
Beta strandi171 – 1733Combined sources
Beta strandi175 – 1773Combined sources
Beta strandi179 – 1813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LMZNMR-A1-187[»]
1NKUNMR-A1-187[»]
1P7MNMR-A1-187[»]
ProteinModelPortaliP05100.
SMRiP05100. Positions 1-187.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05100.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108R3E. Bacteria.
COG2818. LUCA.
HOGENOMiHOG000220753.
InParanoidiP05100.
KOiK01246.
OMAiFCGPTIV.
PhylomeDBiP05100.

Family and domain databases

Gene3Di1.10.340.30. 1 hit.
InterProiIPR005019. Adenine_glyco.
IPR011257. DNA_glycosylase.
IPR004597. Tag.
[Graphical view]
PfamiPF03352. Adenine_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR00624. tag. 1 hit.

Sequencei

Sequence statusi: Complete.

P05100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERCGWVSQD PLYIAYHDNE WGVPETDSKK LFEMICLEGQ QAGLSWITVL
60 70 80 90 100
KKRENYRACF HQFDPVKVAA MQEEDVERLV QDAGIIRHRG KIQAIIGNAR
110 120 130 140 150
AYLQMEQNGE PFVDFVWSFV NHQPQVTQAT TLSEIPTSTS ASDALSKALK
160 170 180
KRGFKFVGTT ICYSFMQACG LVNDHVVGCC CYPGNKP
Length:187
Mass (Da):21,100
Last modified:August 13, 1987 - v1
Checksum:i5A305B5CA66A48FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02606 Genomic DNA. Translation: AAA24658.1.
X03845 Genomic DNA. Translation: CAA27472.1.
U00039 Genomic DNA. Translation: AAB18526.1.
U00096 Genomic DNA. Translation: AAC76573.1.
AP009048 Genomic DNA. Translation: BAE77746.1.
PIRiA24604. DGECM1.
RefSeqiNP_418005.1. NC_000913.3.
WP_000438957.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76573; AAC76573; b3549.
BAE77746; BAE77746; BAE77746.
GeneIDi947137.
KEGGiecj:JW3518.
eco:b3549.
PATRICi32122570. VBIEscCol129921_3662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02606 Genomic DNA. Translation: AAA24658.1.
X03845 Genomic DNA. Translation: CAA27472.1.
U00039 Genomic DNA. Translation: AAB18526.1.
U00096 Genomic DNA. Translation: AAC76573.1.
AP009048 Genomic DNA. Translation: BAE77746.1.
PIRiA24604. DGECM1.
RefSeqiNP_418005.1. NC_000913.3.
WP_000438957.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1LMZNMR-A1-187[»]
1NKUNMR-A1-187[»]
1P7MNMR-A1-187[»]
ProteinModelPortaliP05100.
SMRiP05100. Positions 1-187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261555. 184 interactions.
DIPiDIP-10950N.
IntActiP05100. 5 interactions.
MINTiMINT-1230071.
STRINGi511145.b3549.

Chemistry

ChEMBLiCHEMBL2295563.

Proteomic databases

EPDiP05100.
PaxDbiP05100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76573; AAC76573; b3549.
BAE77746; BAE77746; BAE77746.
GeneIDi947137.
KEGGiecj:JW3518.
eco:b3549.
PATRICi32122570. VBIEscCol129921_3662.

Organism-specific databases

EchoBASEiEB0979.
EcoGeneiEG10986. tag.

Phylogenomic databases

eggNOGiENOG4108R3E. Bacteria.
COG2818. LUCA.
HOGENOMiHOG000220753.
InParanoidiP05100.
KOiK01246.
OMAiFCGPTIV.
PhylomeDBiP05100.

Enzyme and pathway databases

BioCyciEcoCyc:EG10986-MONOMER.
ECOL316407:JW3518-MONOMER.
MetaCyc:EG10986-MONOMER.
RETL1328306-WGS:GSTH-825-MONOMER.
BRENDAi3.2.2.20. 2026.

Miscellaneous databases

EvolutionaryTraceiP05100.
PROiP05100.

Family and domain databases

Gene3Di1.10.340.30. 1 hit.
InterProiIPR005019. Adenine_glyco.
IPR011257. DNA_glycosylase.
IPR004597. Tag.
[Graphical view]
PfamiPF03352. Adenine_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR00624. tag. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei3MG1_ECOLI
AccessioniPrimary (citable) accession number: P05100
Secondary accession number(s): Q2M7L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.