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Protein

Leucoagglutinating phytohemagglutinin

Gene

DLEC2

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This insecticidal carbohydrate-binding lectin is toxic for the cowpea weevil.

Miscellaneous

Antibiosis properties of legume lectins are proposed to be due to the lysis of epithelial cells of the intestine by binding to the carbohydrate moieties of these proteins.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionToxin
Biological processPlant defense
LigandLectin, Mannose-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucoagglutinating phytohemagglutinin
Short name:
PHA-L
Gene namesi
Name:DLEC2
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Pathology & Biotechi

Protein family/group databases

Allergomei6155. Pha v PHA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
ChainiPRO_000001763421 – 272Leucoagglutinating phytohemagglutininAdd BLAST252

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
GlycosylationiCAR_00012132N-linked (GlcNAc...) (high mannose) asparagine1 Publication1
GlycosylationiCAR_00012280N-linked (GlcNAc...) (complex) asparagine1 Publication1

Post-translational modificationi

N-glycosylated on Asn-80; contains xylose.1 Publication

Keywords - PTMi

Glycoprotein

PTM databases

UniCarbKBiP05087.

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1272
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 30Combined sources7
Helixi33 – 35Combined sources3
Beta strandi36 – 40Combined sources5
Beta strandi66 – 76Combined sources11
Turni80 – 83Combined sources4
Beta strandi84 – 95Combined sources12
Beta strandi106 – 114Combined sources9
Helixi123 – 125Combined sources3
Turni126 – 128Combined sources3
Beta strandi139 – 144Combined sources6
Turni149 – 151Combined sources3
Beta strandi157 – 166Combined sources10
Beta strandi168 – 172Combined sources5
Beta strandi180 – 188Combined sources9
Turni189 – 192Combined sources4
Beta strandi193 – 200Combined sources8
Turni201 – 204Combined sources4
Beta strandi205 – 212Combined sources8
Helixi215 – 218Combined sources4
Beta strandi221 – 231Combined sources11
Beta strandi242 – 252Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FATX-ray2.80A/B/C/D21-272[»]
1G8WX-ray2.80A/B/C/D21-253[»]
ProteinModelPortaliP05087.
SMRiP05087.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05087.

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd06899. lectin_legume_LecRK_Arcelin_Co. 1 hit.
InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
PfamiView protein in Pfam
PF00139. Lectin_legB. 1 hit.
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSKFFTVL FLVLLTHANS SNDIYFNFQR FNETNLILQR DASVSSSGQL
60 70 80 90 100
RLTNLNGNGE PRVGSLGRAF YSAPIQIWDN TTGTVASFAT SFTFNIQVPN
110 120 130 140 150
NAGPADGLAF ALVPVGSQPK DKGGFLGLFD GSNSNFHTVA VEFDTLYNKD
160 170 180 190 200
WDPTERHIGI DVNSIRSIKT TRWDFVNGEN AEVLITYDSS TNLLVASLVY
210 220 230 240 250
PSQKTSFIVS DTVDLKSVLP EWVSVGFSAT TGINKGNVET NDVLSWSFAS
260 270
KLSDGTTSEG LNLANLVLNK IL
Length:272
Mass (Da):29,556
Last modified:August 13, 1987 - v1
Checksum:i9DBF7D3077A067D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02409 Genomic DNA. Translation: CAA26257.1.
K03289 Genomic DNA. Translation: AAA33760.1.
PIRiB22826.
RefSeqiXP_007152772.1. XM_007152710.1.

Genome annotation databases

GeneIDi18633199.
KEGGipvu:PHAVU_004G158300g.

Similar proteinsi

Entry informationi

Entry nameiPHAL_PHAVU
AccessioniPrimary (citable) accession number: P05087
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: August 30, 2017
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families