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Protein

Leucoagglutinating phytohemagglutinin

Gene

DLEC2

Organism
Phaseolus vulgaris (Kidney bean) (French bean)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This insecticidal carbohydrate-binding lectin is toxic for the cowpea weevil.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Plant defense

Keywords - Ligandi

Lectin, Mannose-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucoagglutinating phytohemagglutinin
Short name:
PHA-L
Gene namesi
Name:DLEC2
OrganismiPhaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifieri3885 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePhaseolus

Pathology & Biotechi

Protein family/group databases

Allergomei6155. Pha v PHA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Add
BLAST
Chaini21 – 272252Leucoagglutinating phytohemagglutininPRO_0000017634Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...) (high mannose)1 PublicationCAR_000121
Glycosylationi80 – 801N-linked (GlcNAc...) (complex)1 PublicationCAR_000122

Post-translational modificationi

N-glycosylated on Asn-80; contains xylose.1 Publication

Keywords - PTMi

Glycoprotein

PTM databases

UniCarbKBiP05087.

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
272
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 307Combined sources
Helixi33 – 353Combined sources
Beta strandi36 – 405Combined sources
Beta strandi66 – 7611Combined sources
Turni80 – 834Combined sources
Beta strandi84 – 9512Combined sources
Beta strandi106 – 1149Combined sources
Helixi123 – 1253Combined sources
Turni126 – 1283Combined sources
Beta strandi139 – 1446Combined sources
Turni149 – 1513Combined sources
Beta strandi157 – 16610Combined sources
Beta strandi168 – 1725Combined sources
Beta strandi180 – 1889Combined sources
Turni189 – 1924Combined sources
Beta strandi193 – 2008Combined sources
Turni201 – 2044Combined sources
Beta strandi205 – 2128Combined sources
Helixi215 – 2184Combined sources
Beta strandi221 – 23111Combined sources
Beta strandi242 – 25211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FATX-ray2.80A/B/C/D21-272[»]
1G8WX-ray2.80A/B/C/D21-253[»]
ProteinModelPortaliP05087.
SMRiP05087. Positions 21-253.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05087.

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSKFFTVL FLVLLTHANS SNDIYFNFQR FNETNLILQR DASVSSSGQL
60 70 80 90 100
RLTNLNGNGE PRVGSLGRAF YSAPIQIWDN TTGTVASFAT SFTFNIQVPN
110 120 130 140 150
NAGPADGLAF ALVPVGSQPK DKGGFLGLFD GSNSNFHTVA VEFDTLYNKD
160 170 180 190 200
WDPTERHIGI DVNSIRSIKT TRWDFVNGEN AEVLITYDSS TNLLVASLVY
210 220 230 240 250
PSQKTSFIVS DTVDLKSVLP EWVSVGFSAT TGINKGNVET NDVLSWSFAS
260 270
KLSDGTTSEG LNLANLVLNK IL
Length:272
Mass (Da):29,556
Last modified:August 13, 1987 - v1
Checksum:i9DBF7D3077A067D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02409 Genomic DNA. Translation: CAA26257.1.
K03289 Genomic DNA. Translation: AAA33760.1.
PIRiB22826.
RefSeqiXP_007152772.1. XM_007152710.1.

Genome annotation databases

GeneIDi18633199.
KEGGipvu:PHAVU_004G158300g.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - Glycan Binding

PHA-L

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02409 Genomic DNA. Translation: CAA26257.1.
K03289 Genomic DNA. Translation: AAA33760.1.
PIRiB22826.
RefSeqiXP_007152772.1. XM_007152710.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FATX-ray2.80A/B/C/D21-272[»]
1G8WX-ray2.80A/B/C/D21-253[»]
ProteinModelPortaliP05087.
SMRiP05087. Positions 21-253.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei6155. Pha v PHA.

PTM databases

UniCarbKBiP05087.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18633199.
KEGGipvu:PHAVU_004G158300g.

Miscellaneous databases

EvolutionaryTraceiP05087.
PROiP05087.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHAL_PHAVU
AccessioniPrimary (citable) accession number: P05087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: June 8, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Antibiosis properties of legume lectins are proposed to be due to the lysis of epithelial cells of the intestine by binding to the carbohydrate moieties of these proteins.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.