P05085 (ARGR2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine metabolism regulation protein II

Alternative name(s):
Arginine-requiring protein 81

Gene names

Name:	ARG81
Synonyms:	ARGR2
Ordered Locus Names:	YML099C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	880 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	With ARG80, ARG82 and MCM1, coordinates the expression of arginine anabolic and catabolic genes in response to arginine. Ref.5 Ref.6
Subunit structure	Interacts with ARG80 and MCM1. Ref.10
Subcellular location	Cytoplasm. Nucleus Ref.4 Ref.11.
Sequence similarities	Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Ontologies

Keywords
Biological process	Arginine metabolism Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Traceable author statement Ref.5. Source: SGD negative regulation of calcium ion-dependent exocytosis Inferred from direct assay. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription cofactor activity Inferred from direct assay. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 880	880	Arginine metabolism regulation protein II		PRO_0000114937
Regions
	DNA binding	21 – 48	28	Zn(2)-C6 fungal-type Ref.7 Ref.8 Ref.9
Amino acid modifications
	Modified residue	177	1	Phosphoserine Ref.12
Experimental info
	Sequence conflict	4	1	S → F in CAA27577. Ref.1
	Sequence conflict	129	1	Missing in CAA27577. Ref.1
	Sequence conflict	283	1	F → V in CAA27577. Ref.1
	Sequence conflict	345	1	D → V in CAA27577. Ref.1
	Sequence conflict	366	1	E → Q in CAA27577. Ref.1
	Sequence conflict	549	1	T → A in CAA27577. Ref.1
	Sequence conflict	597	1	T → S in CAA27577. Ref.1
	Sequence conflict	665	1	K → N in CAA27577. Ref.1
	Sequence conflict	869	1	V → I in CAA27577. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P05085 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: DA033AB2B373F415	FASTA	880	100,282
	10 20 30 40 50 60 MGISSKNGPK KMGRAKTFTG CWTCRGRKVK CDLRHPHCQR CEKSNLPCGG YDIKLRWSKP 70 80 90 100 110 120 MQFDPYGVPI PQNSPATTTN LSGSVDEPQY QRRNIDFVRY DEEYVYHEDM DDELTMLHTP 130 140 150 160 170 180 PIEKISDNKT WIIKKFGVFK GTDKIDKQYA PRKKRNRKRV AKSLESSASI SLSSLPSSST 190 200 210 220 230 240 ISFPIRHIED KLRNKGHVKT GILSANDGVP PTPNLLDYDW NNLNITGYEW ISSELRDDAL 250 260 270 280 290 300 LSAVTLQGHH LGHTQPQEIS LEENSNVVSG EEHVNAKEHG CAFEADNQGS STLPNKAASA 310 320 330 340 350 360 NDKLYQQNLK LLFQKNSSNS EEPDPQALID DVFVNIEPRS LPASDLNKIT LAPPNEESRM 370 380 390 400 410 420 PKSMLELTSY SSDLPPELVD IIPKTDLTVH GLARFLLNHY FNNVADKMTV VVLEKNPWKT 430 440 450 460 470 480 LYFPRALMAL GDLAGLGQSS NSRNALLNAL LAVSCFHLQS KYPRNYKLQK YFLGLGIELR 490 500 510 520 530 540 NQASNFLRLC LNTKSSIPEK YKDVLTAILS MNSIDVVWGT MADCQDHLAL CEDFVESRMK 550 560 570 580 590 600 LRPNISEKTK TLHRIFSFLK LIQDSTALDK VRAKEIVILP SEEDDNYKPL DTSNATTSSS 610 620 630 640 650 660 EPRVDVVQEG LFREALNEND GKIHIEFVKE PITNVSADST PSSTTPPIFT NIATESYYNK 670 680 690 700 710 720 SDISKLVSKT DENIIGTDSL YGLPNSLILL FSDCVRIVRH NEYYNLTYLP VPRKFNELSL 730 740 750 760 770 780 NFEKRLLKWK SEWNFHQENS EGKSFINSTA EALYHHTMSF YFSLIIYYFT MARSLNCQFL 790 800 810 820 830 840 QNYVAKVLDH LNAMEELVDQ KKVKIVPLIW QGFMAGCACT DENRQQEFRR WAAKLAESGV 850 860 870 880 GSYWGARQVM LEVWRRRKED EPGDNWYSVY KDWEMNLMLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the ARGRII regulatory gene and amino acid sequence homologies between ARGRII PPRI and GAL4 regulatory proteins." Messenguy F., Dubois E., Descamps F. Eur. J. Biochem. 157:77-81(1986) [PubMed: 3709534] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Regulation of arginine metabolism in Saccharomyces cerevisiae: expression of the three ARGR regulatory genes and cellular localization of their products." Bercy J., Dubois E., Messenguy F. Gene 55:277-285(1987) [PubMed: 3311884] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Characterization of two genes, ARGRI and ARGRIII required for specific regulation of arginine metabolism in yeast." Dubois E., Bercy J., Messenguy F. Mol. Gen. Genet. 207:142-148(1987) [PubMed: 3298999] [Abstract] Cited for: FUNCTION.
[6]	"Functional analysis of ARGRI and ARGRIII regulatory proteins involved in the regulation of arginine metabolism in Saccharomyces cerevisiae." Qiu H.F., Dubois E., Broen P., Messenguy F. Mol. Gen. Genet. 222:192-200(1990) [PubMed: 2274024] [Abstract] Cited for: FUNCTION.
[7]	"In vitro studies of the binding of the ARGR proteins to the ARG5,6 promoter." Dubois E., Messenguy F. Mol. Cell. Biol. 11:2162-2168(1991) [PubMed: 2005902] [Abstract] Cited for: DNA-BINDING.
[8]	"Dissection of the bifunctional ARGRII protein involved in the regulation of arginine anabolic and catabolic pathways." Qui H.F., Dubois E., Messenguy F. Mol. Cell. Biol. 11:2169-2179(1991) [PubMed: 2005903] [Abstract] Cited for: DNA-BINDING.
[9]	"Determination of the DNA-binding sequences of ARGR proteins to arginine anabolic and catabolic promoters." Messenguy F., Dubois E., Boonchird C. Mol. Cell. Biol. 11:2852-2863(1991) [PubMed: 2017180] [Abstract] Cited for: DNA-BINDING.
[10]	"Recruitment of the yeast MADS-box proteins, ArgRI and Mcm1 by the pleiotropic factor ArgRIII is required for their stability." El Bakkoury M., Dubois E., Messenguy F. Mol. Microbiol. 35:15-31(2000) [PubMed: 10632874] [Abstract] Cited for: INTERACTION WITH ARG80 AND MCM1.
[11]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-177, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03940 Genomic DNA. Translation: CAA27577.1. Z46660 Genomic DNA. Translation: CAA86638.1. BK006946 Genomic DNA. Translation: DAA09800.1.
PIR	S49627.
RefSeq	NP_013610.1. NM_001182459.1.
3D structure databases
ProteinModelPortal	P05085.
SMR	P05085. Positions 13-49.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1346N.
IntAct	P05085. 12 interactions.
MINT	MINT-412052.
STRING	P05085.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML099C; YML099C; YML099C.
GeneID	854874.
KEGG	sce:YML099C.
NMPDR	fig|4932.3.peg.4647.
Organism-specific databases
CYGD	YML099c.
SGD	S000004565. ARG81.
Phylogenomic databases
eggNOG	fuNOG04357.
GeneTree	EFGT00050000001217.
HOGENOM	HBG395762.
OMA	DWEMNLM.
OrthoDB	EOG48D43X.
Gene expression databases
ArrayExpress	P05085.
Genevestigator	P05085.
GermOnline	YML099C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021858. DUF3468. IPR001138. Zn2-C6_fun-type_DNA-bd. [Graphical view]
Gene3D	G3DSA:4.10.240.10. Zn2-C6_fun-type_DNA-bd. 1 hit.
KO	K09246.
Pfam	PF11951. DUF3468. 1 hit. PF00172. Zn_clus. 1 hit. [Graphical view]
SMART	SM00066. GAL4. 1 hit. [Graphical view]
SUPFAM	SSF57701. Fungi_TrN. 1 hit.
PROSITE	PS00463. ZN2_CY6_FUNGAL_1. 1 hit. PS50048. ZN2_CY6_FUNGAL_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977810.

Entry information

Entry name

ARGR2_YEAST

Accession

Primary (citable) accession number: P05085
Secondary accession number(s): D6W0I6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1988
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program