Reviewed, UniProtKB/Swiss-Prot P05081 (KAD1_CHICK)

Names and origin

Protein names

Recommended name:
    Adenylate kinase isoenzyme 1
      Short name=AK 1
    EC=2.7.4.3
Alternative name(s):
    ATP-AMP transphosphorylase 1
    Myokinase

Gene names

Name:

AK1

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth.
Catalytic activity	ATP + AMP = 2 ADP.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the adenylate kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	ATP metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Adenylate kinase isoenzyme 1		PRO_0000158915
Regions
	Nucleotide binding	16 – 24	9	ATP By similarity
	Nucleotide binding	95 – 102	8	AMP By similarity
Sites
	Binding site	40	1	AMP By similarity
Experimental info
	Mutagenesis	22	1	K → M: Reduced activity. Ref.3
	Mutagenesis	28	1	K → M: No loss in activity. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P05081-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 7DCAEF3E1DD474EC	FASTA	194	21,683
	10 20 30 40 50 60 MSTEKLKHHK IIFVVGGPGS GKGTQCEKIV HKYGYTHLST GDLLRAEVSS GSERGKKLQA 70 80 90 100 110 120 IMEKGELVPL DTVLDMLRDA MLAKADTSKG FLIDGYPREV KQGEEFEKKI APPTLLLYVD 130 140 150 160 170 180 AGKETMVKRL LKRGETSGRV DDNEETIKKR LETYYKATEP VIAFYKGRGI VRQLNAEGTV 190 DEVFQQVCSY LDKL

References

[1]	"Isolation and characterization of cDNA for chicken muscle adenylate kinase." Kishi F., Maruyama M., Tanizawa Y., Nakazawa A. J. Biol. Chem. 261:2942-2945(1986) [PubMed: 2419325] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.
[2]	"Structure and complete nucleotide sequence of the gene encoding chicken cytosolic adenylate kinase." Suminami Y., Kishi F., Torigoe T., Nakazawa A. J. Biochem. 103:611-617(1988) [PubMed: 2844738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Muscle.
[3]	"Mechanism of adenylate kinase. Are the essential lysines essential?" Tian G.C., Yan H.G., Jiang R.T., Kishi F., Nakazawa A., Tsai M.D. Biochemistry 29:4296-4304(1990) [PubMed: 2161682] [Abstract] Cited for: MUTAGENESIS OF LYS-22 AND LYS-28.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M12153 mRNA. Translation: AAB59961.1. D00251 Genomic DNA. Translation: BAA00182.1.
IPI	IPI00571711.
PIR	A25327.
RefSeq	NP_990440.1.
UniGene	Gga.4422
3D structure databases
HSSP	HSSP built from PDB template 3ADK based on UniProtKB P00571.
SMR	P05081. Positions 4-194.
ModBase	Search...
Genome annotation databases
GeneID	396002.
KEGG	gga:396002.
Phylogenomic databases
HOVERGEN	P05081.
Enzyme and pathway databases
BRENDA	2.7.4.3. 4.
Family and domain databases
InterPro	IPR000850. Adenylate_kin. IPR006267. Adenylate_kin1. [Graphical view]
PANTHER	PTHR23359. Adenylate_kin. 1 hit.
Pfam	PF00406. ADK. 1 hit. [Graphical view]
PRINTS	PR00094. ADENYLTKNASE.
ProDom	PD000657. Adenylate_kin. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01360. aden_kin_iso1. 1 hit.
PROSITE	PS00113. ADENYLATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAD1_CHICK

Accession

Primary (citable) accession number: P05081

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)