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P05068 (CR1AC_BACTK) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pesticidal crystal protein cry1Ac
Alternative name(s):
133 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(c)
Gene names
Name:cry1Ac
Synonyms:cry1A(c), cry218, cryIA(c)
Encoded onPlasmid 75 Kb
OrganismBacillus thuringiensis subsp. kurstaki
Taxonomic identifier29339 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length1178 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

Developmental stage

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Biotechnological use

Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Sequence similarities

Belongs to the delta endotoxin family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11781178Pesticidal crystal protein cry1Ac
PRO_0000174024

Secondary structure

...................................................................................... 1178
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P05068 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 9B34F2ED410E88BB

FASTA1,178133,331
        10         20         30         40         50         60 
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL 

        70         80         90        100        110        120 
VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD 

       130        140        150        160        170        180 
PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ 

       190        200        210        220        230        240 
RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV 

       250        260        270        280        290        300 
LDIVALFPNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL 

       310        320        330        340        350        360 
NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR 

       370        380        390        400        410        420 
TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV 

       430        440        450        460        470        480 
PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIASDS ITQIPAVKGN 

       490        500        510        520        530        540 
FLFNGSVISG PGFTGGDLVR LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH 

       550        560        570        580        590        600 
LNVNWGNSSI FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII 

       610        620        630        640        650        660 
DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS NLVTYLSDEF 

       670        680        690        700        710        720 
CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG WGGSTGITIQ GGDDVFKENY 

       730        740        750        760        770        780 
VTLSGTFDEC YPTYLYQKID ESKLKAFTRY QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG 

       790        800        810        820        830        840 
TGSLWPLSAQ SPIGKCGEPN RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL 

       850        860        870        880        890        900 
NEDLGVWVIF KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI 

       910        920        930        940        950        960 
VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV IPGVNAAIFE 

       970        980        990       1000       1010       1020 
ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE EQNNQRSVLV VPEWEAEVSQ 

      1030       1040       1050       1060       1070       1080 
EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE IENNTDELKF SNCVEEEIYP NNTVTCNDYT 

      1090       1100       1110       1120       1130       1140 
VNQEEYGGAY TSRNRGYNEA PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG 

      1150       1160       1170 
YVTKELEYFP ETDKVWIEIG ETEGTFIVDS VELLLMEE 

« Hide

References

[1]"Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta."
Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F., Thompson D.V.
Gene 36:289-300(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-73.
[2]"Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus."
Omolo E.O., James M.D., Osir E.O., Thomson J.A.
Curr. Microbiol. 34:118-121(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-73.
[3]Herrera G., James M.D.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HD-73.
[4]"Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae)."
Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.
N. Z. J. Crop Hortic. Sci. 20:27-36(1992)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSIR732.
[5]Sun M., Yu Z.
Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YBT-1520.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11068 Genomic DNA. Translation: AAA22331.1.
U87397 Genomic DNA. Translation: AAC44841.1.
U87793 Genomic DNA. Translation: AAB46989.1.
U89872 Genomic DNA. Translation: AAB49768.1.
AJ002514 Genomic DNA. Translation: CAA05505.1.
PIRUSBSXH. A23962.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4ARXX-ray2.35A/B/C/D31-609[»]
4ARYX-ray2.95A/B/C/D31-611[»]
ProteinModelPortalP05068.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCR1AC_BACTK
AccessionPrimary (citable) accession number: P05068
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 16, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references