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P05068

- CR1AC_BACTK

UniProt

P05068 - CR1AC_BACTK

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Protein

Pesticidal crystal protein cry1Ac

Gene

cry1Ac

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: InterPro
  3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pesticidal crystal protein cry1Ac
Alternative name(s):
133 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(c)
Gene namesi
Name:cry1Ac
Synonyms:cry1A(c), cry218, cryIA(c)
Encoded oniPlasmid 75 Kb0 Publication
OrganismiBacillus thuringiensis subsp. kurstaki
Taxonomic identifieri29339 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Biotechnological usei

Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11781178Pesticidal crystal protein cry1AcPRO_0000174024Add
BLAST

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Structurei

Secondary structure

1
1178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 4814
Beta strandi51 – 533
Helixi54 – 6310
Helixi71 – 8414
Helixi90 – 11930
Helixi124 – 14421
Helixi145 – 1484
Turni154 – 1574
Helixi158 – 18225
Helixi186 – 21732
Helixi223 – 23917
Helixi241 – 2444
Helixi245 – 2506
Turni252 – 2543
Beta strandi266 – 2694
Helixi271 – 2744
Helixi284 – 2896
Beta strandi298 – 31013
Beta strandi313 – 32513
Helixi326 – 3283
Beta strandi344 – 3518
Beta strandi358 – 36811
Beta strandi379 – 39012
Beta strandi399 – 4024
Beta strandi406 – 4083
Helixi409 – 4113
Helixi422 – 4254
Beta strandi428 – 43811
Turni441 – 4433
Beta strandi445 – 45713
Beta strandi468 – 4758
Helixi476 – 4783
Beta strandi480 – 4889
Beta strandi492 – 4965
Beta strandi498 – 5014
Helixi508 – 5103
Beta strandi513 – 5175
Beta strandi527 – 5348
Beta strandi539 – 5457
Beta strandi548 – 5558
Beta strandi562 – 5643
Helixi567 – 5693
Beta strandi571 – 5744
Helixi581 – 5833
Beta strandi585 – 5917
Beta strandi598 – 60710

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ARXX-ray2.35A/B/C/D31-609[»]
4ARYX-ray2.95A/B/C/D31-611[»]
ProteinModelPortaliP05068.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the delta endotoxin family.Curated

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.

Sequencei

Sequence statusi: Complete.

P05068-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF
60 70 80 90 100
VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL
110 120 130 140 150
EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV
160 170 180 190 200
QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI
210 220 230 240 250
GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV LDIVALFPNY
260 270 280 290 300
DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL
310 320 330 340 350
NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI
360 370 380 390 400
VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV
410 420 430 440 450
YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA
460 470 480 490 500
PMFSWIHRSA EFNNIIASDS ITQIPAVKGN FLFNGSVISG PGFTGGDLVR
510 520 530 540 550
LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH LNVNWGNSSI
560 570 580 590 600
FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII
610 620 630 640 650
DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS
660 670 680 690 700
NLVTYLSDEF CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG
710 720 730 740 750
WGGSTGITIQ GGDDVFKENY VTLSGTFDEC YPTYLYQKID ESKLKAFTRY
760 770 780 790 800
QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG TGSLWPLSAQ SPIGKCGEPN
810 820 830 840 850
RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL NEDLGVWVIF
860 870 880 890 900
KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI
910 920 930 940 950
VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV
960 970 980 990 1000
IPGVNAAIFE ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE
1010 1020 1030 1040 1050
EQNNQRSVLV VPEWEAEVSQ EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE
1060 1070 1080 1090 1100
IENNTDELKF SNCVEEEIYP NNTVTCNDYT VNQEEYGGAY TSRNRGYNEA
1110 1120 1130 1140 1150
PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG YVTKELEYFP
1160 1170
ETDKVWIEIG ETEGTFIVDS VELLLMEE
Length:1,178
Mass (Da):133,331
Last modified:August 13, 1987 - v1
Checksum:i9B34F2ED410E88BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1.
U87397 Genomic DNA. Translation: AAC44841.1.
U87793 Genomic DNA. Translation: AAB46989.1.
U89872 Genomic DNA. Translation: AAB49768.1.
AJ002514 Genomic DNA. Translation: CAA05505.1.
PIRiA23962. USBSXH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1 .
U87397 Genomic DNA. Translation: AAC44841.1 .
U87793 Genomic DNA. Translation: AAB46989.1 .
U89872 Genomic DNA. Translation: AAB49768.1 .
AJ002514 Genomic DNA. Translation: CAA05505.1 .
PIRi A23962. USBSXH.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4ARX X-ray 2.35 A/B/C/D 31-609 [» ]
4ARY X-ray 2.95 A/B/C/D 31-611 [» ]
ProteinModelPortali P05068.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProi IPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view ]
Pfami PF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta."
    Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F., Thompson D.V.
    Gene 36:289-300(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  2. "Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus."
    Omolo E.O., James M.D., Osir E.O., Thomson J.A.
    Curr. Microbiol. 34:118-121(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  3. Herrera G., James M.D.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  4. "Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae)."
    Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.
    N. Z. J. Crop Hortic. Sci. 20:27-36(1992)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSIR732.
  5. Sun M., Yu Z.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YBT-1520.

Entry informationi

Entry nameiCR1AC_BACTK
AccessioniPrimary (citable) accession number: P05068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 1, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Keywords - Technical termi

3D-structure, Genetically modified food, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3