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Protein

Pesticidal crystal protein Cry1Ac

Gene

cry1Ac

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pesticidal crystal protein Cry1Ac
Alternative name(s):
133 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(c)
Gene namesi
Name:cry1Ac
Synonyms:cry1A(c), cry218, cryIA(c)
Encoded oniPlasmid 75 Kb0 Publication
OrganismiBacillus thuringiensis subsp. kurstaki
Taxonomic identifieri29339 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Biotechnological usei

Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001740241 – 1178Pesticidal crystal protein Cry1AcAdd BLAST1178

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Structurei

Secondary structure

11178
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 48Combined sources14
Beta strandi51 – 53Combined sources3
Helixi54 – 63Combined sources10
Turni64 – 67Combined sources4
Helixi71 – 84Combined sources14
Helixi90 – 119Combined sources30
Helixi124 – 144Combined sources21
Helixi145 – 148Combined sources4
Turni154 – 157Combined sources4
Helixi158 – 182Combined sources25
Helixi186 – 217Combined sources32
Helixi223 – 239Combined sources17
Helixi241 – 244Combined sources4
Helixi245 – 250Combined sources6
Turni252 – 254Combined sources3
Beta strandi266 – 269Combined sources4
Helixi271 – 274Combined sources4
Helixi284 – 289Combined sources6
Beta strandi298 – 310Combined sources13
Beta strandi313 – 325Combined sources13
Helixi326 – 328Combined sources3
Beta strandi344 – 351Combined sources8
Beta strandi358 – 368Combined sources11
Beta strandi379 – 390Combined sources12
Beta strandi399 – 402Combined sources4
Beta strandi406 – 408Combined sources3
Helixi409 – 411Combined sources3
Beta strandi418 – 420Combined sources3
Helixi422 – 425Combined sources4
Beta strandi428 – 438Combined sources11
Turni441 – 443Combined sources3
Beta strandi445 – 457Combined sources13
Beta strandi468 – 475Combined sources8
Helixi476 – 478Combined sources3
Beta strandi480 – 488Combined sources9
Beta strandi492 – 496Combined sources5
Beta strandi498 – 501Combined sources4
Helixi508 – 510Combined sources3
Beta strandi513 – 517Combined sources5
Beta strandi527 – 534Combined sources8
Beta strandi539 – 545Combined sources7
Beta strandi548 – 555Combined sources8
Beta strandi562 – 564Combined sources3
Helixi567 – 569Combined sources3
Beta strandi571 – 574Combined sources4
Helixi581 – 583Combined sources3
Beta strandi585 – 591Combined sources7
Beta strandi598 – 607Combined sources10
Helixi610 – 626Combined sources17
Beta strandi629 – 631Combined sources3
Helixi643 – 654Combined sources12
Turni658 – 660Combined sources3
Helixi662 – 682Combined sources21
Beta strandi705 – 711Combined sources7
Beta strandi720 – 724Combined sources5
Beta strandi733 – 739Combined sources7
Helixi741 – 743Combined sources3
Beta strandi746 – 778Combined sources33
Beta strandi827 – 834Combined sources8
Turni840 – 842Combined sources3
Beta strandi846 – 852Combined sources7
Beta strandi854 – 856Combined sources3
Beta strandi858 – 871Combined sources14
Helixi874 – 910Combined sources37
Turni917 – 919Combined sources3
Helixi927 – 937Combined sources11
Helixi956 – 976Combined sources21
Turni985 – 990Combined sources6
Beta strandi998 – 1003Combined sources6
Beta strandi1006 – 1011Combined sources6
Beta strandi1018 – 1023Combined sources6
Beta strandi1030 – 1049Combined sources20
Beta strandi1051 – 1053Combined sources3
Beta strandi1056 – 1061Combined sources6
Beta strandi1142 – 1148Combined sources7
Beta strandi1151 – 1175Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ARXX-ray2.35A/B/C/D31-609[»]
4ARYX-ray2.95A/B/C/D31-611[»]
4W8JX-ray2.78A1-1178[»]
ProteinModelPortaliP05068.
SMRiP05068.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the delta endotoxin family.Curated

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.

Sequencei

Sequence statusi: Complete.

P05068-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF
60 70 80 90 100
VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL
110 120 130 140 150
EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV
160 170 180 190 200
QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI
210 220 230 240 250
GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV LDIVALFPNY
260 270 280 290 300
DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL
310 320 330 340 350
NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI
360 370 380 390 400
VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV
410 420 430 440 450
YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA
460 470 480 490 500
PMFSWIHRSA EFNNIIASDS ITQIPAVKGN FLFNGSVISG PGFTGGDLVR
510 520 530 540 550
LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH LNVNWGNSSI
560 570 580 590 600
FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII
610 620 630 640 650
DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS
660 670 680 690 700
NLVTYLSDEF CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG
710 720 730 740 750
WGGSTGITIQ GGDDVFKENY VTLSGTFDEC YPTYLYQKID ESKLKAFTRY
760 770 780 790 800
QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG TGSLWPLSAQ SPIGKCGEPN
810 820 830 840 850
RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL NEDLGVWVIF
860 870 880 890 900
KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI
910 920 930 940 950
VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV
960 970 980 990 1000
IPGVNAAIFE ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE
1010 1020 1030 1040 1050
EQNNQRSVLV VPEWEAEVSQ EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE
1060 1070 1080 1090 1100
IENNTDELKF SNCVEEEIYP NNTVTCNDYT VNQEEYGGAY TSRNRGYNEA
1110 1120 1130 1140 1150
PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG YVTKELEYFP
1160 1170
ETDKVWIEIG ETEGTFIVDS VELLLMEE
Length:1,178
Mass (Da):133,331
Last modified:August 13, 1987 - v1
Checksum:i9B34F2ED410E88BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1.
U87397 Genomic DNA. Translation: AAC44841.1.
U87793 Genomic DNA. Translation: AAB46989.1.
U89872 Genomic DNA. Translation: AAB49768.1.
AJ002514 Genomic DNA. Translation: CAA05505.1.
PIRiA23962. USBSXH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1.
U87397 Genomic DNA. Translation: AAC44841.1.
U87793 Genomic DNA. Translation: AAB46989.1.
U89872 Genomic DNA. Translation: AAB49768.1.
AJ002514 Genomic DNA. Translation: CAA05505.1.
PIRiA23962. USBSXH.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4ARXX-ray2.35A/B/C/D31-609[»]
4ARYX-ray2.95A/B/C/D31-611[»]
4W8JX-ray2.78A1-1178[»]
ProteinModelPortaliP05068.
SMRiP05068.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 2 hits.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCR1AC_BACTK
AccessioniPrimary (citable) accession number: P05068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 30, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Keywords - Technical termi

3D-structure, Genetically modified food, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.