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P05068

- CR1AC_BACTK

UniProt

P05068 - CR1AC_BACTK

Protein

Pesticidal crystal protein cry1Ac

Gene

cry1Ac

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

    GO - Biological processi

    1. defense response Source: InterPro
    2. pathogenesis Source: InterPro
    3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Molecular functioni

    Toxin

    Keywords - Biological processi

    Sporulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pesticidal crystal protein cry1Ac
    Alternative name(s):
    133 kDa crystal protein
    Crystaline entomocidal protoxin
    Insecticidal delta-endotoxin CryIA(c)
    Gene namesi
    Name:cry1Ac
    Synonyms:cry1A(c), cry218, cryIA(c)
    Encoded oniPlasmid 75 Kb0 Publication
    OrganismiBacillus thuringiensis subsp. kurstaki
    Taxonomic identifieri29339 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

    Pathology & Biotechi

    Biotechnological usei

    Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11781178Pesticidal crystal protein cry1AcPRO_0000174024Add
    BLAST

    Expressioni

    Developmental stagei

    The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

    Structurei

    Secondary structure

    1
    1178
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi35 – 4814
    Beta strandi51 – 533
    Helixi54 – 6310
    Helixi71 – 8414
    Helixi90 – 11930
    Helixi124 – 14421
    Helixi145 – 1484
    Turni154 – 1574
    Helixi158 – 18225
    Helixi186 – 21732
    Helixi223 – 23917
    Helixi241 – 2444
    Helixi245 – 2506
    Turni252 – 2543
    Beta strandi266 – 2694
    Helixi271 – 2744
    Helixi284 – 2896
    Beta strandi298 – 31013
    Beta strandi313 – 32513
    Helixi326 – 3283
    Beta strandi344 – 3518
    Beta strandi358 – 36811
    Beta strandi379 – 39012
    Beta strandi399 – 4024
    Beta strandi406 – 4083
    Helixi409 – 4113
    Helixi422 – 4254
    Beta strandi428 – 43811
    Turni441 – 4433
    Beta strandi445 – 45713
    Beta strandi468 – 4758
    Helixi476 – 4783
    Beta strandi480 – 4889
    Beta strandi492 – 4965
    Beta strandi498 – 5014
    Helixi508 – 5103
    Beta strandi513 – 5175
    Beta strandi527 – 5348
    Beta strandi539 – 5457
    Beta strandi548 – 5558
    Beta strandi562 – 5643
    Helixi567 – 5693
    Beta strandi571 – 5744
    Helixi581 – 5833
    Beta strandi585 – 5917
    Beta strandi598 – 60710

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4ARXX-ray2.35A/B/C/D31-609[»]
    4ARYX-ray2.95A/B/C/D31-611[»]
    ProteinModelPortaliP05068.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the delta endotoxin family.Curated

    Family and domain databases

    Gene3Di1.20.190.10. 1 hit.
    2.100.10.10. 1 hit.
    2.60.120.260. 1 hit.
    InterProiIPR005638. Endotoxin_C.
    IPR001178. Endotoxin_cen_dom.
    IPR015790. Endotoxin_cen_dom_subgr1.
    IPR005639. Endotoxin_N.
    IPR008979. Galactose-bd-like.
    [Graphical view]
    PfamiPF03944. Endotoxin_C. 1 hit.
    PF00555. Endotoxin_M. 1 hit.
    PF03945. Endotoxin_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51096. SSF51096. 1 hit.
    SSF56849. SSF56849. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P05068-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF     50
    VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL 100
    EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV 150
    QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI 200
    GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV LDIVALFPNY 250
    DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL 300
    NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI 350
    VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV 400
    YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA 450
    PMFSWIHRSA EFNNIIASDS ITQIPAVKGN FLFNGSVISG PGFTGGDLVR 500
    LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH LNVNWGNSSI 550
    FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII 600
    DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS 650
    NLVTYLSDEF CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG 700
    WGGSTGITIQ GGDDVFKENY VTLSGTFDEC YPTYLYQKID ESKLKAFTRY 750
    QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG TGSLWPLSAQ SPIGKCGEPN 800
    RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL NEDLGVWVIF 850
    KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI 900
    VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV 950
    IPGVNAAIFE ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE 1000
    EQNNQRSVLV VPEWEAEVSQ EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE 1050
    IENNTDELKF SNCVEEEIYP NNTVTCNDYT VNQEEYGGAY TSRNRGYNEA 1100
    PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG YVTKELEYFP 1150
    ETDKVWIEIG ETEGTFIVDS VELLLMEE 1178
    Length:1,178
    Mass (Da):133,331
    Last modified:August 13, 1987 - v1
    Checksum:i9B34F2ED410E88BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11068 Genomic DNA. Translation: AAA22331.1.
    U87397 Genomic DNA. Translation: AAC44841.1.
    U87793 Genomic DNA. Translation: AAB46989.1.
    U89872 Genomic DNA. Translation: AAB49768.1.
    AJ002514 Genomic DNA. Translation: CAA05505.1.
    PIRiA23962. USBSXH.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M11068 Genomic DNA. Translation: AAA22331.1 .
    U87397 Genomic DNA. Translation: AAC44841.1 .
    U87793 Genomic DNA. Translation: AAB46989.1 .
    U89872 Genomic DNA. Translation: AAB49768.1 .
    AJ002514 Genomic DNA. Translation: CAA05505.1 .
    PIRi A23962. USBSXH.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4ARX X-ray 2.35 A/B/C/D 31-609 [» ]
    4ARY X-ray 2.95 A/B/C/D 31-611 [» ]
    ProteinModelPortali P05068.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.190.10. 1 hit.
    2.100.10.10. 1 hit.
    2.60.120.260. 1 hit.
    InterProi IPR005638. Endotoxin_C.
    IPR001178. Endotoxin_cen_dom.
    IPR015790. Endotoxin_cen_dom_subgr1.
    IPR005639. Endotoxin_N.
    IPR008979. Galactose-bd-like.
    [Graphical view ]
    Pfami PF03944. Endotoxin_C. 1 hit.
    PF00555. Endotoxin_M. 1 hit.
    PF03945. Endotoxin_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51096. SSF51096. 1 hit.
    SSF56849. SSF56849. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta."
      Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F., Thompson D.V.
      Gene 36:289-300(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-73.
    2. "Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus."
      Omolo E.O., James M.D., Osir E.O., Thomson J.A.
      Curr. Microbiol. 34:118-121(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-73.
    3. Herrera G., James M.D.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HD-73.
    4. "Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae)."
      Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.
      N. Z. J. Crop Hortic. Sci. 20:27-36(1992)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSIR732.
    5. Sun M., Yu Z.
      Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YBT-1520.

    Entry informationi

    Entry nameiCR1AC_BACTK
    AccessioniPrimary (citable) accession number: P05068
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Toxic segment of the protein is located in the N-terminus.

    Keywords - Technical termi

    3D-structure, Genetically modified food, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3