SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P05068

- CR1AC_BACTK

UniProt

P05068 - CR1AC_BACTK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Pesticidal crystal protein cry1Ac
Gene
cry1Ac, cry1A(c), cry218, cryIA(c)
Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: InterPro
  3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pesticidal crystal protein cry1Ac
Alternative name(s):
133 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(c)
Gene namesi
Name:cry1Ac
Synonyms:cry1A(c), cry218, cryIA(c)
Encoded oniPlasmid 75 Kb0 Publication
OrganismiBacillus thuringiensis subsp. kurstaki
Taxonomic identifieri29339 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Biotechnological usei

Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11781178Pesticidal crystal protein cry1Ac
PRO_0000174024Add
BLAST

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 4814
Beta strandi51 – 533
Helixi54 – 6310
Helixi71 – 8414
Helixi90 – 11930
Helixi124 – 14421
Helixi145 – 1484
Turni154 – 1574
Helixi158 – 18225
Helixi186 – 21732
Helixi223 – 23917
Helixi241 – 2444
Helixi245 – 2506
Turni252 – 2543
Beta strandi266 – 2694
Helixi271 – 2744
Helixi284 – 2896
Beta strandi298 – 31013
Beta strandi313 – 32513
Helixi326 – 3283
Beta strandi344 – 3518
Beta strandi358 – 36811
Beta strandi379 – 39012
Beta strandi399 – 4024
Beta strandi406 – 4083
Helixi409 – 4113
Helixi422 – 4254
Beta strandi428 – 43811
Turni441 – 4433
Beta strandi445 – 45713
Beta strandi468 – 4758
Helixi476 – 4783
Beta strandi480 – 4889
Beta strandi492 – 4965
Beta strandi498 – 5014
Helixi508 – 5103
Beta strandi513 – 5175
Beta strandi527 – 5348
Beta strandi539 – 5457
Beta strandi548 – 5558
Beta strandi562 – 5643
Helixi567 – 5693
Beta strandi571 – 5744
Helixi581 – 5833
Beta strandi585 – 5917
Beta strandi598 – 60710

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ARXX-ray2.35A/B/C/D31-609[»]
4ARYX-ray2.95A/B/C/D31-611[»]
ProteinModelPortaliP05068.

Family & Domainsi

Sequence similaritiesi

Belongs to the delta endotoxin family.

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.

Sequencei

Sequence statusi: Complete.

P05068-1 [UniParc]FASTAAdd to Basket

« Hide

MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF     50
VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL 100
EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV 150
QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI 200
GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV LDIVALFPNY 250
DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL 300
NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI 350
VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV 400
YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA 450
PMFSWIHRSA EFNNIIASDS ITQIPAVKGN FLFNGSVISG PGFTGGDLVR 500
LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH LNVNWGNSSI 550
FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII 600
DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS 650
NLVTYLSDEF CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG 700
WGGSTGITIQ GGDDVFKENY VTLSGTFDEC YPTYLYQKID ESKLKAFTRY 750
QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG TGSLWPLSAQ SPIGKCGEPN 800
RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL NEDLGVWVIF 850
KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI 900
VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV 950
IPGVNAAIFE ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE 1000
EQNNQRSVLV VPEWEAEVSQ EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE 1050
IENNTDELKF SNCVEEEIYP NNTVTCNDYT VNQEEYGGAY TSRNRGYNEA 1100
PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG YVTKELEYFP 1150
ETDKVWIEIG ETEGTFIVDS VELLLMEE 1178
Length:1,178
Mass (Da):133,331
Last modified:August 13, 1987 - v1
Checksum:i9B34F2ED410E88BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1.
U87397 Genomic DNA. Translation: AAC44841.1.
U87793 Genomic DNA. Translation: AAB46989.1.
U89872 Genomic DNA. Translation: AAB49768.1.
AJ002514 Genomic DNA. Translation: CAA05505.1.
PIRiA23962. USBSXH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1 .
U87397 Genomic DNA. Translation: AAC44841.1 .
U87793 Genomic DNA. Translation: AAB46989.1 .
U89872 Genomic DNA. Translation: AAB49768.1 .
AJ002514 Genomic DNA. Translation: CAA05505.1 .
PIRi A23962. USBSXH.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4ARX X-ray 2.35 A/B/C/D 31-609 [» ]
4ARY X-ray 2.95 A/B/C/D 31-611 [» ]
ProteinModelPortali P05068.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProi IPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view ]
Pfami PF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta."
    Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F., Thompson D.V.
    Gene 36:289-300(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  2. "Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus."
    Omolo E.O., James M.D., Osir E.O., Thomson J.A.
    Curr. Microbiol. 34:118-121(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  3. Herrera G., James M.D.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  4. "Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae)."
    Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.
    N. Z. J. Crop Hortic. Sci. 20:27-36(1992)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSIR732.
  5. Sun M., Yu Z.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YBT-1520.

Entry informationi

Entry nameiCR1AC_BACTK
AccessioniPrimary (citable) accession number: P05068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 16, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Keywords - Technical termi

3D-structure, Genetically modified food, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi