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Protein

Pesticidal crystal protein cry1Ac

Gene

cry1Ac

Organism
Bacillus thuringiensis subsp. kurstaki
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.

GO - Biological processi

  1. defense response Source: InterPro
  2. pathogenesis Source: InterPro
  3. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Toxin

Keywords - Biological processi

Sporulation

Names & Taxonomyi

Protein namesi
Recommended name:
Pesticidal crystal protein cry1Ac
Alternative name(s):
133 kDa crystal protein
Crystaline entomocidal protoxin
Insecticidal delta-endotoxin CryIA(c)
Gene namesi
Name:cry1Ac
Synonyms:cry1A(c), cry218, cryIA(c)
Encoded oniPlasmid 75 Kb0 Publication
OrganismiBacillus thuringiensis subsp. kurstaki
Taxonomic identifieri29339 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Biotechnological usei

Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11781178Pesticidal crystal protein cry1AcPRO_0000174024Add
BLAST

Expressioni

Developmental stagei

The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.

Structurei

Secondary structure

1
1178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 4814Combined sources
Beta strandi51 – 533Combined sources
Helixi54 – 6310Combined sources
Turni64 – 674Combined sources
Helixi71 – 8414Combined sources
Helixi90 – 11930Combined sources
Helixi124 – 14421Combined sources
Helixi145 – 1484Combined sources
Turni154 – 1574Combined sources
Helixi158 – 18225Combined sources
Helixi186 – 21732Combined sources
Helixi223 – 23917Combined sources
Helixi241 – 2444Combined sources
Helixi245 – 2506Combined sources
Turni252 – 2543Combined sources
Beta strandi266 – 2694Combined sources
Helixi271 – 2744Combined sources
Helixi284 – 2896Combined sources
Beta strandi298 – 31013Combined sources
Beta strandi313 – 32513Combined sources
Helixi326 – 3283Combined sources
Beta strandi344 – 3518Combined sources
Beta strandi358 – 36811Combined sources
Beta strandi379 – 39012Combined sources
Beta strandi399 – 4024Combined sources
Beta strandi406 – 4083Combined sources
Helixi409 – 4113Combined sources
Beta strandi418 – 4203Combined sources
Helixi422 – 4254Combined sources
Beta strandi428 – 43811Combined sources
Turni441 – 4433Combined sources
Beta strandi445 – 45713Combined sources
Beta strandi468 – 4758Combined sources
Helixi476 – 4783Combined sources
Beta strandi480 – 4889Combined sources
Beta strandi492 – 4965Combined sources
Beta strandi498 – 5014Combined sources
Helixi508 – 5103Combined sources
Beta strandi513 – 5175Combined sources
Beta strandi527 – 5348Combined sources
Beta strandi539 – 5457Combined sources
Beta strandi548 – 5558Combined sources
Beta strandi562 – 5643Combined sources
Helixi567 – 5693Combined sources
Beta strandi571 – 5744Combined sources
Helixi581 – 5833Combined sources
Beta strandi585 – 5917Combined sources
Beta strandi598 – 60710Combined sources
Helixi610 – 62617Combined sources
Beta strandi629 – 6313Combined sources
Helixi643 – 65412Combined sources
Turni658 – 6603Combined sources
Helixi662 – 68221Combined sources
Beta strandi705 – 7117Combined sources
Beta strandi720 – 7245Combined sources
Beta strandi733 – 7397Combined sources
Helixi741 – 7433Combined sources
Beta strandi746 – 77833Combined sources
Beta strandi827 – 8348Combined sources
Turni840 – 8423Combined sources
Beta strandi846 – 8527Combined sources
Beta strandi854 – 8563Combined sources
Beta strandi858 – 87114Combined sources
Helixi874 – 91037Combined sources
Turni917 – 9193Combined sources
Helixi927 – 93711Combined sources
Helixi956 – 97621Combined sources
Turni985 – 9906Combined sources
Beta strandi998 – 10036Combined sources
Beta strandi1006 – 10116Combined sources
Beta strandi1018 – 10236Combined sources
Beta strandi1030 – 104920Combined sources
Beta strandi1051 – 10533Combined sources
Beta strandi1056 – 10616Combined sources
Beta strandi1142 – 11487Combined sources
Beta strandi1151 – 117525Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ARXX-ray2.35A/B/C/D31-609[»]
4ARYX-ray2.95A/B/C/D31-611[»]
4W8JX-ray2.78A1-1178[»]
ProteinModelPortaliP05068.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the delta endotoxin family.Curated

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.

Sequencei

Sequence statusi: Complete.

P05068-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF
60 70 80 90 100
VPGAGFVLGL VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL
110 120 130 140 150
EGLSNLYQIY AESFREWEAD PTNPALREEM RIQFNDMNSA LTTAIPLFAV
160 170 180 190 200
QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ RWGFDAATIN SRYNDLTRLI
210 220 230 240 250
GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV LDIVALFPNY
260 270 280 290 300
DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL
310 320 330 340 350
NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI
360 370 380 390 400
VAQLGQGVYR TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV
410 420 430 440 450
YRKSGTVDSL DEIPPQNNNV PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA
460 470 480 490 500
PMFSWIHRSA EFNNIIASDS ITQIPAVKGN FLFNGSVISG PGFTGGDLVR
510 520 530 540 550
LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH LNVNWGNSSI
560 570 580 590 600
FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII
610 620 630 640 650
DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS
660 670 680 690 700
NLVTYLSDEF CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG
710 720 730 740 750
WGGSTGITIQ GGDDVFKENY VTLSGTFDEC YPTYLYQKID ESKLKAFTRY
760 770 780 790 800
QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG TGSLWPLSAQ SPIGKCGEPN
810 820 830 840 850
RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL NEDLGVWVIF
860 870 880 890 900
KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI
910 920 930 940 950
VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV
960 970 980 990 1000
IPGVNAAIFE ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE
1010 1020 1030 1040 1050
EQNNQRSVLV VPEWEAEVSQ EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE
1060 1070 1080 1090 1100
IENNTDELKF SNCVEEEIYP NNTVTCNDYT VNQEEYGGAY TSRNRGYNEA
1110 1120 1130 1140 1150
PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG YVTKELEYFP
1160 1170
ETDKVWIEIG ETEGTFIVDS VELLLMEE
Length:1,178
Mass (Da):133,331
Last modified:August 13, 1987 - v1
Checksum:i9B34F2ED410E88BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1.
U87397 Genomic DNA. Translation: AAC44841.1.
U87793 Genomic DNA. Translation: AAB46989.1.
U89872 Genomic DNA. Translation: AAB49768.1.
AJ002514 Genomic DNA. Translation: CAA05505.1.
PIRiA23962. USBSXH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11068 Genomic DNA. Translation: AAA22331.1.
U87397 Genomic DNA. Translation: AAC44841.1.
U87793 Genomic DNA. Translation: AAB46989.1.
U89872 Genomic DNA. Translation: AAB49768.1.
AJ002514 Genomic DNA. Translation: CAA05505.1.
PIRiA23962. USBSXH.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4ARXX-ray2.35A/B/C/D31-609[»]
4ARYX-ray2.95A/B/C/D31-611[»]
4W8JX-ray2.78A1-1178[»]
ProteinModelPortaliP05068.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.190.10. 1 hit.
2.100.10.10. 1 hit.
2.60.120.260. 1 hit.
InterProiIPR005638. Endotoxin_C.
IPR001178. Endotoxin_cen_dom.
IPR015790. Endotoxin_cen_dom_subgr1.
IPR005639. Endotoxin_N.
IPR008979. Galactose-bd-like.
[Graphical view]
PfamiPF03944. Endotoxin_C. 1 hit.
PF00555. Endotoxin_M. 1 hit.
PF03945. Endotoxin_N. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51096. SSF51096. 1 hit.
SSF56849. SSF56849. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta."
    Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F., Thompson D.V.
    Gene 36:289-300(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  2. "Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus."
    Omolo E.O., James M.D., Osir E.O., Thomson J.A.
    Curr. Microbiol. 34:118-121(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  3. Herrera G., James M.D.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HD-73.
  4. "Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae)."
    Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J.
    N. Z. J. Crop Hortic. Sci. 20:27-36(1992)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSIR732.
  5. Sun M., Yu Z.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YBT-1520.

Entry informationi

Entry nameiCR1AC_BACTK
AccessioniPrimary (citable) accession number: P05068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Toxic segment of the protein is located in the N-terminus.

Keywords - Technical termi

3D-structure, Genetically modified food, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.