Reviewed, UniProtKB/Swiss-Prot P05068 (CR1AC_BACTK)

Names and origin

Protein names

Recommended name:
    Pesticidal crystal protein cry1Ac
Alternative name(s):
    Insecticidal delta-endotoxin CryIA(c)
    Crystaline entomocidal protoxin
    133 kDa crystal protein

Gene names

Name:	cry1Ac
Synonyms:	cry1A(c), cry218, cryIA(c)

Encoded on

Plasmid 75 Kb

Organism

Bacillus thuringiensis subsp. kurstaki

Taxonomic identifier

29339 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	1178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Promotes colloidosmotic lysis by binding to the midgut epithelial cells of many lepidopteran larvae.
Developmental stage	The crystal protein is produced during sporulation and is accumulated both as an inclusion and as part of the spore coat.
Biotechnological use	Introduced by genetic manipulation and expressed in insect-resistant cotton and tomato by Calgene (Monsanto) and in maize by Dekalb Genetics.
Miscellaneous	Toxic segment of the protein is located in the N-terminus.
Sequence similarities	Belongs to the delta endotoxin family.

Ontologies

Keywords
Biological process	Sporulation
Molecular function	Toxin
Technical term	Genetically modified food Plasmid
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1178	1178	Pesticidal crystal protein cry1Ac		PRO_0000174024

Sequences

	Sequence		Length	Mass (Da)
	P05068-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 9B34F2ED410E88BB	FASTA	1,178	133,331
	10 20 30 40 50 60 MDNNPNINEC IPYNCLSNPE VEVLGGERIE TGYTPIDISL SLTQFLLSEF VPGAGFVLGL 70 80 90 100 110 120 VDIIWGIFGP SQWDAFLVQI EQLINQRIEE FARNQAISRL EGLSNLYQIY AESFREWEAD 130 140 150 160 170 180 PTNPALREEM RIQFNDMNSA LTTAIPLFAV QNYQVPLLSV YVQAANLHLS VLRDVSVFGQ 190 200 210 220 230 240 RWGFDAATIN SRYNDLTRLI GNYTDYAVRW YNTGLERVWG PDSRDWVRYN QFRRELTLTV 250 260 270 280 290 300 LDIVALFPNY DSRRYPIRTV SQLTREIYTN PVLENFDGSF RGSAQGIERS IRSPHLMDIL 310 320 330 340 350 360 NSITIYTDAH RGYYYWSGHQ IMASPVGFSG PEFTFPLYGT MGNAAPQQRI VAQLGQGVYR 370 380 390 400 410 420 TLSSTLYRRP FNIGINNQQL SVLDGTEFAY GTSSNLPSAV YRKSGTVDSL DEIPPQNNNV 430 440 450 460 470 480 PPRQGFSHRL SHVSMFRSGF SNSSVSIIRA PMFSWIHRSA EFNNIIASDS ITQIPAVKGN 490 500 510 520 530 540 FLFNGSVISG PGFTGGDLVR LNSSGNNIQN RGYIEVPIHF PSTSTRYRVR VRYASVTPIH 550 560 570 580 590 600 LNVNWGNSSI FSNTVPATAT SLDNLQSSDF GYFESANAFT SSLGNIVGVR NFSGTAGVII 610 620 630 640 650 660 DRFEFIPVTA TLEAEYNLER AQKAVNALFT STNQLGLKTN VTDYHIDQVS NLVTYLSDEF 670 680 690 700 710 720 CLDEKRELSE KVKHAKRLSD ERNLLQDSNF KDINRQPERG WGGSTGITIQ GGDDVFKENY 730 740 750 760 770 780 VTLSGTFDEC YPTYLYQKID ESKLKAFTRY QLRGYIEDSQ DLEIYLIRYN AKHETVNVPG 790 800 810 820 830 840 TGSLWPLSAQ SPIGKCGEPN RCAPHLEWNP DLDCSCRDGE KCAHHSHHFS LDIDVGCTDL 850 860 870 880 890 900 NEDLGVWVIF KIKTQDGHAR LGNLEFLEEK PLVGEALARV KRAEKKWRDK REKLEWETNI 910 920 930 940 950 960 VYKEAKESVD ALFVNSQYDQ LQADTNIAMI HAADKRVHSI REAYLPELSV IPGVNAAIFE 970 980 990 1000 1010 1020 ELEGRIFTAF SLYDARNVIK NGDFNNGLSC WNVKGHVDVE EQNNQRSVLV VPEWEAEVSQ 1030 1040 1050 1060 1070 1080 EVRVCPGRGY ILRVTAYKEG YGEGCVTIHE IENNTDELKF SNCVEEEIYP NNTVTCNDYT 1090 1100 1110 1120 1130 1140 VNQEEYGGAY TSRNRGYNEA PSVPADYASV YEEKSYTDGR RENPCEFNRG YRDYTPLPVG 1150 1160 1170 YVTKELEYFP ETDKVWIEIG ETEGTFIVDS VELLLMEE

References

[1]	"Characterized full-length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD-73 and their toxicity to Manduca sexta." Adang M.J., Staver M.J., Rocheleau T.A., Leighton J., Barker R.F., Thompson D.V. Gene 36:289-300(1985) [PubMed: 3000881] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HD-73.
[2]	"Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus." Omolo E.O., James M.D., Osir E.O., Thomson J.A. Curr. Microbiol. 34:118-121(1997) [PubMed: 9003589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HD-73.
[3]	Herrera G., James M.D. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: HD-73.
[4]	"Cloning and nucleotide sequence of an insecticidal crystal protein gene from Bacillus thuringiensis DSIR732 active against 3 species of leafroller (Lepidoptera, Tortricidae)." Gleave A.P., Hedges R.J., Broadwell A.H., Wigley P.J. N. Z. J. Crop Hortic. Sci. 20:27-36(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSIR732.
[5]	Sun M., Yu Z. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YBT-1520.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M11068 Genomic DNA. Translation: AAA22331.1. U87397 Genomic DNA. Translation: AAC44841.1. U87793 Genomic DNA. Translation: AAB46989.1. U89872 Genomic DNA. Translation: AAB49768.1. AJ002514 Genomic DNA. Translation: CAA05505.1.
PIR	USBSXH. A23962.
3D structure databases
HSSP	HSSP built from PDB template 1CIY based on UniProtKB P02965.
ModBase	Search...
Family and domain databases
InterPro	IPR005638. Endotoxin_C. IPR001178. Endotoxin_M. IPR015790. Endotoxin_m_sub_1. IPR005639. Endotoxin_N. [Graphical view]
Gene3D	G3DSA:2.100.10.10. Endotoxin_M. 1 hit. G3DSA:1.20.190.10. Endotoxin_N. 1 hit.
Pfam	PF03944. Endotoxin_C. 1 hit. PF00555. Endotoxin_M. 1 hit. PF03945. Endotoxin_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CR1AC_BACTK

Accession

Primary (citable) accession number: P05068

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)