Reviewed,
UniProtKB/Swiss-Prot P05065 (ALDOA_RAT)
Last modified
November 3, 2009.
Version 89.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Fructose-bisphosphate aldolase A EC=4.1.2.13 Alternative name(s): Muscle-type aldolase | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 364 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. |
| Pathway | |
| Subunit structure | Homotetramer. |
| Tissue specificity | Expressed in muscle, brain and hepatoma cells. Ref.6 |
| Miscellaneous | In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. |
| Sequence similarities | Belongs to the class I fructose-bisphosphate aldolase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glycolysis |
| Ligand | Schiff base |
| Molecular function | Lyase |
| PTM | Acetylation Phosphoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: UniProtKB-KW response to estrogen stimulusInferred from expression pattern. Source: RGD response to heatInferred from expression pattern. Source: RGD response to hypoxiaInferred from expression pattern. Source: RGD response to lipopolysaccharideInferred from expression pattern. Source: RGD response to nicotineInferred from expression pattern. Source: RGD |
| Cellular component | mitochondrion Inferred from direct assay. Source: RGD |
| Molecular function | fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 364 | 363 | Fructose-bisphosphate aldolase A | PRO_0000216939 | |||||
Sites | |||||||||
| Active site | 188 | 1 | Proton acceptor By similarity | ||||||
| Active site | 230 | 1 | Schiff-base intermediate with dihydroxyacetone-P | ||||||
| Binding site | 56 | 1 | Substrate | ||||||
| Binding site | 147 | 1 | Substrate | ||||||
| Site | 364 | 1 | Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate | ||||||
Amino acid modifications | |||||||||
| Modified residue | 5 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 13 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 36 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 39 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 42 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 46 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 65 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 108 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 204 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 223 | 1 | Phosphotyrosine By similarity | ||||||
| Modified residue | 235 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 241 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 330 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 354 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 356 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 364 | 1 | Phosphotyrosine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 145 | 1 | F → S in AAA40715. Ref.2 | ||||||
| Sequence conflict | 165 | 1 | M → V in AAA40715. Ref.2 | ||||||
| Sequence conflict | 330 | 1 | K → Q in AAA40720. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Tissue-specific expression of rat aldolase A mRNAs. Three molecular species differing only in the 5'-terminal sequences." Mukai T., Joh K., Arai Y., Yatsuki H., Hori K. J. Biol. Chem. 261:3347-3354(1986) [PubMed: 3753977] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Rat aldolase A messenger RNA: the nucleotide sequence and multiple mRNA species with different 5'-terminal regions." Joh K., Mukai T., Yatsuki H., Hori K. Gene 39:17-24(1985) [PubMed: 2416636] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "Expression of three mRNA species from a single rat aldolase A gene, differing in their 5' non-coding regions." Joh K., Arai Y., Mukai T., Hori K. J. Mol. Biol. 190:401-410(1986) [PubMed: 3783705] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate. |
| [5] | Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-13; 15-22; 29-42; 70-99; 154-173; 209-215; 244-258; 290-312 AND 332-364, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Spinal cord. |
| [6] | "Two different aldolase A mRNA species in rat tissues." Tsutsumi R., Tsutsumi K., Numazaki M., Ishikawa K. Eur. J. Biochem. 142:161-164(1984) [PubMed: 6086339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 324-356, TISSUE SPECIFICITY. Strain: Donryu. Tissue: Hepatoma. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M12919 mRNA. Translation: AAA40714.1. M14420 mRNA. Translation: AAA40715.1. X04261 X04264 Genomic DNA. Translation: CAA27815.1. BC064440 mRNA. Translation: AAH64440.1. M28282 mRNA. Translation: AAA40720.1. | |
| IPI | IPI00231734. |
| PIR | ADRTA. A24532. |
| RefSeq | NP_036627.1. |
| UniGene | Rn.1774 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1ADO based on UniProtKB P00883. |
| SMR | P05065. Positions 2-364. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P05065. |
PTM databases | |
| PhosphoSite | P05065. |
2-D gel databases | |
| Rat-heart-2DPAGE | P05065. |
Proteomic databases | |
| PRIDE | P05065. |
Genome annotation databases | |
| Ensembl | ENSRNOT00000032264; ENSRNOP00000031940; ENSRNOG00000023647; Rattus norvegicus. [Genome view] ENSRNOT00000032362; ENSRNOP00000032320; ENSRNOG00000023647; Rattus norvegicus. [Genome view] |
| GeneID | 24189. |
| KEGG | rno:24189. |
| UCSC | NM_012495. rat. |
Organism-specific databases | |
| CTD | 24189. |
| RGD | 2089. Aldoa. |
Phylogenomic databases | |
| HOVERGEN | P05065. |
| OMA | AHLSAMN. |
Enzyme and pathway databases | |
| BRENDA | 4.1.2.13. 248. |
Gene expression databases | |
| ArrayExpress | P05065. |
| Genevestigator | P05065. |
| GermOnline | ENSRNOG00000023647. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view] |
| Gene3D | G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. |
| PANTHER | PTHR11627. Aldolase_I. 1 hit. |
| Pfam | PF00274. Glycolytic. 1 hit. [Graphical view] |
| ProDom | PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| PROSITE | PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 602559. |
Entry information
| Entry name | ALDOA_RAT | ||||||||
| Accession | Primary (citable) accession number: P05065 Secondary accession number(s): Q63038 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


