Reviewed, UniProtKB/Swiss-Prot P05064 (ALDOA_MOUSE)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase A
    EC=4.1.2.13
Alternative name(s):
    Muscle-type aldolase
    Aldolase 1

Gene names

Name:	Aldoa
Synonyms:	Aldo1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subunit structure	Homotetramer.
Miscellaneous	In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
PTM	Acetylation Nitration Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	flagellum Inferred from direct assay. Source: MGI
Molecular function	fructose-bisphosphate aldolase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 364	363	Fructose-bisphosphate aldolase A		PRO_0000216937
Sites
	Active site	188	1	Proton acceptor By similarity
	Active site	230	1	Schiff-base intermediate with dihydroxyacetone-P
	Binding site	56	1	Substrate
	Binding site	147	1	Substrate
	Site	364	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate
Amino acid modifications
	Modified residue	5	1	Phosphotyrosine By similarity
	Modified residue	13	1	N6-acetyllysine By similarity
	Modified residue	36	1	Phosphoserine Ref.6 Ref.8
	Modified residue	39	1	Phosphoserine Ref.8
	Modified residue	42	1	N6-acetyllysine By similarity
	Modified residue	46	1	Phosphoserine By similarity
	Modified residue	65	1	Phosphothreonine By similarity
	Modified residue	174	1	Nitrated tyrosine
	Modified residue	204	1	Phosphotyrosine By similarity
	Modified residue	223	1	Phosphotyrosine By similarity
	Modified residue	235	1	Phosphothreonine By similarity
	Modified residue	241	1	Phosphothreonine By similarity
	Modified residue	354	1	Phosphoserine By similarity
	Modified residue	356	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	281	1	S → C in CAA27423. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P05064-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0D067F7E4C63E216	FASTA	364	39,356
	10 20 30 40 50 60 MPHPYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR 70 80 90 100 110 120 QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN 130 140 150 160 170 180 GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ 190 200 210 220 230 240 NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC 250 260 270 280 290 300 TQKFSNEEIA MATVTALRRT VPPAVTGVTF LSGGQSEEEA SINLNAINKC PLLKPWALTF 310 320 330 340 350 360 SYGRALQASA LKAWGGKKEN LKAAQEEYIK RALANSLACQ GKYTPSGQSG AAASESLFIS NHAY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a mouse brain aldolase A cDNA." Mestek A., Stauffer J., Tolan D.R., Ciejek-Baez E. Nucleic Acids Res. 15:10595-10595(1987) [PubMed: 3697100] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"Nonconservative utilization of aldolase A alternative promoters." Stauffer J.K., Colbert M.C., Ciejek-Baez E. J. Biol. Chem. 265:11773-11782(1990) [PubMed: 2365699] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-266 AND 295-364.
[4]	Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-22; 29-57; 61-109; 112-134; 154-258; 260-312 AND 323-364, MASS SPECTROMETRY. Strain: C57BL/6 and OF1. Tissue: Brain and Hippocampus.
[5]	"Structure and expression of mouse aldolase genes. Brain-specific aldolase C amino acid sequence is closely related to aldolase A." Paolella G., Buono P., Mancini P., Izzo P., Salvatore F. Eur. J. Biochem. 156:229-235(1986) [PubMed: 3009179] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 99-355.
[6]	"Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L., Hart G.W., Burlingame A.L. Proteomics 5:388-398(2005) [PubMed: 15648052] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY. Tissue: Brain.
[7]	"Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease." Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C., Bigelow D.J. Biochemistry 45:8009-8022(2006) [PubMed: 16800626] [Abstract] Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-174, MASS SPECTROMETRY. Tissue: Brain.
[8]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36 AND SER-39, MASS SPECTROMETRY. Tissue: Brain cortex.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X03797 mRNA. Translation: CAA27423.1. BC043026 mRNA. Translation: AAH43026.1. BC050896 mRNA. Translation: AAH50896.1. J05517 Genomic DNA. Translation: AAA37210.2. Y00516 mRNA. Translation: CAA68571.1.
IPI	IPI00221402.
PIR	ADMSA. S06323.
RefSeq	NP_031464.1.
UniGene	Mm.275831
3D structure databases
HSSP	HSSP built from PDB template 1ADO based on UniProtKB P00883.
SMR	P05064. Positions 2-364.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05064. 4 interactions.
PTM databases
PhosphoSite	P05064.
2-D gel databases
SWISS-2DPAGE	P05064.
REPRODUCTION-2DPAGE	P05064.
Proteomic databases
PRIDE	P05064.
Genome annotation databases
Ensembl	ENSMUSG00000030695. Mus musculus. [Contig view]
GeneID	11674.
KEGG	mmu:11674.
Organism-specific databases
MGI	MGI:87994. Aldoa.
Phylogenomic databases
HOGENOM	P05064.
HOVERGEN	P05064.
Enzyme and pathway databases
BRENDA	4.1.2.13. 244.
Gene expression databases
ArrayExpress	P05064.
Bgee	P05064.
CleanEx	MM_ALDOA.
GermOnline	ENSMUSG00000030695. Mus musculus.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	279303.
SOURCE	Search...

Entry information

Entry name

ALDOA_MOUSE

Accession

Primary (citable) accession number: P05064

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)