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Reviewed, UniProtKB/Swiss-Prot P05064 (ALDOA_MOUSE)

Last modified June 16, 2009. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Fructose-bisphosphate aldolase A
    EC=4.1.2.13
Alternative name(s):
    Muscle-type aldolase
    Aldolase 1
Gene names
Name: Aldoa
Synonyms: Aldo1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length364 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subunit structure

Homotetramer.

Miscellaneous

In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   LigandSchiff base
   Molecular functionLyase
   PTMAcetylation
Nitration
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentflagellum

Inferred from direct assay. Source: MGI

   Molecular functionfructose-bisphosphate aldolase activity

Inferred from direct assay. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 364363Fructose-bisphosphate aldolase A
PRO_0000216937

Sites

Active site1881Proton acceptor By similarity
Active site2301Schiff-base intermediate with dihydroxyacetone-P
Binding site561Substrate
Binding site1471Substrate
Site3641Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

Amino acid modifications

Modified residue51Phosphotyrosine By similarity
Modified residue131N6-acetyllysine By similarity
Modified residue361Phosphoserine Ref.6 Ref.8
Modified residue391Phosphoserine Ref.8
Modified residue421N6-acetyllysine By similarity
Modified residue461Phosphoserine By similarity
Modified residue651Phosphothreonine By similarity
Modified residue1741Nitrated tyrosine
Modified residue2041Phosphotyrosine By similarity
Modified residue2231Phosphotyrosine By similarity
Modified residue2351Phosphothreonine By similarity
Modified residue2411Phosphothreonine By similarity
Modified residue3541Phosphoserine By similarity
Modified residue3561Phosphoserine By similarity

Experimental info

Sequence conflict2811S → C in CAA27423. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P05064-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 0D067F7E4C63E216

FASTA36439,356
        10         20         30         40         50         60 
MPHPYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR 

        70         80         90        100        110        120 
QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN 

       130        140        150        160        170        180 
GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ 

       190        200        210        220        230        240 
NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC 

       250        260        270        280        290        300 
TQKFSNEEIA MATVTALRRT VPPAVTGVTF LSGGQSEEEA SINLNAINKC PLLKPWALTF 

       310        320        330        340        350        360 
SYGRALQASA LKAWGGKKEN LKAAQEEYIK RALANSLACQ GKYTPSGQSG AAASESLFIS 


NHAY 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a mouse brain aldolase A cDNA."
Mestek A., Stauffer J., Tolan D.R., Ciejek-Baez E.
Nucleic Acids Res. 15:10595-10595(1987) [PubMed: 3697100] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Nonconservative utilization of aldolase A alternative promoters."
Stauffer J.K., Colbert M.C., Ciejek-Baez E.
J. Biol. Chem. 265:11773-11782(1990) [PubMed: 2365699] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-266 AND 295-364.
[4]Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-22; 29-57; 61-109; 112-134; 154-258; 260-312 AND 323-364, MASS SPECTROMETRY.
Strain: C57BL/6 and OF1.
Tissue: Brain and Hippocampus.
[5]"Structure and expression of mouse aldolase genes. Brain-specific aldolase C amino acid sequence is closely related to aldolase A."
Paolella G., Buono P., Mancini P., Izzo P., Salvatore F.
Eur. J. Biochem. 156:229-235(1986) [PubMed: 3009179] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 99-355.
[6]"Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol."
Vosseller K., Hansen K.C., Chalkley R.J., Trinidad J.C., Wells L., Hart G.W., Burlingame A.L.
Proteomics 5:388-398(2005) [PubMed: 15648052] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36, MASS SPECTROMETRY.
Tissue: Brain.
[7]"Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease."
Sacksteder C.A., Qian W.-J., Knyushko T.V., Wang H., Chin M.H., Lacan G., Melega W.P., Camp D.G. II, Smith R.D., Smith D.J., Squier T.C., Bigelow D.J.
Biochemistry 45:8009-8022(2006) [PubMed: 16800626] [Abstract]
Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-174, MASS SPECTROMETRY.
Tissue: Brain.
[8]"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-36 AND SER-39, MASS SPECTROMETRY.
Tissue: Brain cortex.
+Additional computationally mapped references.

Cross-references

Sequence databases

X03797 mRNA. Translation: CAA27423.1.
BC043026 mRNA. Translation: AAH43026.1.
BC050896 mRNA. Translation: AAH50896.1.
J05517 Genomic DNA. Translation: AAA37210.2.
Y00516 mRNA. Translation: CAA68571.1.
IPIIPI00221402.
PIRADMSA. S06323.
RefSeqNP_031464.1.
UniGeneMm.275831

3D structure databases

HSSPHSSP built from PDB template 1ADO based on UniProtKB P00883.
SMRP05064. Positions 2-364.
ModBaseSearch...

Protein-protein interaction databases

IntActP05064. 4 interactions.

PTM databases

PhosphoSiteP05064.

2-D gel databases

SWISS-2DPAGEP05064.
REPRODUCTION-2DPAGEP05064.

Proteomic databases

PRIDEP05064.

Genome annotation databases

EnsemblENSMUSG00000030695. Mus musculus. [Contig view]
GeneID11674.
KEGGmmu:11674.

Organism-specific databases

MGIMGI:87994. Aldoa.

Phylogenomic databases

HOGENOMP05064.
HOVERGENP05064.

Enzyme and pathway databases

BRENDA4.1.2.13. 244.

Gene expression databases

ArrayExpressP05064.
BgeeP05064.
CleanExMM_ALDOA.
GermOnlineENSMUSG00000030695. Mus musculus.

Family and domain databases

InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHERPTHR11627. Aldolase_I. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
ProDomPD001128. Aldolase_I. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio279303.
SOURCESearch...

Entry information

Entry nameALDOA_MOUSE
AccessionPrimary (citable) accession number: P05064
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents