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P05064

- ALDOA_MOUSE

UniProt

P05064 - ALDOA_MOUSE

Protein

Fructose-bisphosphate aldolase A

Gene

Aldoa

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein By similarity.By similarity

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei56 – 561Substrate
    Binding sitei147 – 1471Substrate
    Active sitei188 – 1881Proton acceptorBy similarity
    Active sitei230 – 2301Schiff-base intermediate with dihydroxyacetone-P
    Sitei364 – 3641Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB
    2. protease binding Source: UniProtKB

    GO - Biological processi

    1. glycolytic process Source: UniProtKB
    2. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    SABIO-RKP05064.
    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase A (EC:4.1.2.13)
    Alternative name(s):
    Aldolase 1
    Muscle-type aldolase
    Gene namesi
    Name:Aldoa
    Synonyms:Aldo1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:87994. Aldoa.

    Subcellular locationi

    CytoplasmmyofibrilsarcomereI band. CytoplasmmyofibrilsarcomereM line
    Note: In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca2+.By similarity

    GO - Cellular componenti

    1. M band Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB
    3. protein complex Source: UniProtKB
    4. Z disc Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 364363Fructose-bisphosphate aldolase APRO_0000216937Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei36 – 361PhosphoserineBy similarity
    Modified residuei39 – 391PhosphoserineBy similarity
    Modified residuei42 – 421N6-acetyllysineBy similarity
    Modified residuei46 – 461PhosphoserineBy similarity
    Modified residuei108 – 1081N6-acetyllysineBy similarity
    Modified residuei111 – 1111N6-malonyllysineBy similarity
    Modified residuei312 – 3121N6-malonyllysineBy similarity
    Modified residuei330 – 3301N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP05064.
    PaxDbiP05064.
    PRIDEiP05064.

    2D gel databases

    COMPLUYEAST-2DPAGEP05064.
    REPRODUCTION-2DPAGEIPI00221402.
    P05064.
    SWISS-2DPAGEP05064.

    PTM databases

    PhosphoSiteiP05064.

    Expressioni

    Gene expression databases

    ArrayExpressiP05064.
    BgeeiP05064.
    CleanExiMM_ALDOA.
    GenevestigatoriP05064.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with SNX9 and WAS. Interacts with FBP2; the interaction blocks FBP2 inhibition by physiological concentrations of AMP and reduces inhibition by Ca2+ By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198067. 7 interactions.
    IntActiP05064. 20 interactions.
    MINTiMINT-1581489.

    Structurei

    3D structure databases

    ProteinModelPortaliP05064.
    SMRiP05064. Positions 5-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3588.
    HOGENOMiHOG000220876.
    HOVERGENiHBG002386.
    InParanoidiP05064.
    KOiK01623.
    OrthoDBiEOG744T94.
    PhylomeDBiP05064.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]
    PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05064-1 [UniParc]FASTAAdd to Basket

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    MPHPYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE    50
    NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS 100
    KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC 150
    VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK 200
    RCQYVTEKVL AAVYKALSDH HVYLEGTLLK PNMVTPGHAC TQKFSNEEIA 250
    MATVTALRRT VPPAVTGVTF LSGGQSEEEA SINLNAINKC PLLKPWALTF 300
    SYGRALQASA LKAWGGKKEN LKAAQEEYIK RALANSLACQ GKYTPSGQSG 350
    AAASESLFIS NHAY 364
    Length:364
    Mass (Da):39,356
    Last modified:January 23, 2007 - v2
    Checksum:i0D067F7E4C63E216
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti281 – 2811S → C in CAA27423. (PubMed:3009179)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03797 mRNA. Translation: CAA27423.1.
    BC043026 mRNA. Translation: AAH43026.1.
    BC050896 mRNA. Translation: AAH50896.1.
    J05517 Genomic DNA. Translation: AAA37210.2.
    Y00516 mRNA. Translation: CAA68571.1.
    CCDSiCCDS21845.1.
    PIRiS06323. ADMSA.
    RefSeqiNP_001170779.1. NM_001177308.1.
    NP_031464.1. NM_007438.4.
    XP_006507273.1. XM_006507210.1.
    XP_006507274.1. XM_006507211.1.
    UniGeneiMm.275831.

    Genome annotation databases

    EnsembliENSMUST00000032934; ENSMUSP00000032934; ENSMUSG00000030695.
    ENSMUST00000106348; ENSMUSP00000101955; ENSMUSG00000030695.
    GeneIDi11674.
    KEGGimmu:11674.
    UCSCiuc009jsu.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03797 mRNA. Translation: CAA27423.1 .
    BC043026 mRNA. Translation: AAH43026.1 .
    BC050896 mRNA. Translation: AAH50896.1 .
    J05517 Genomic DNA. Translation: AAA37210.2 .
    Y00516 mRNA. Translation: CAA68571.1 .
    CCDSi CCDS21845.1.
    PIRi S06323. ADMSA.
    RefSeqi NP_001170779.1. NM_001177308.1.
    NP_031464.1. NM_007438.4.
    XP_006507273.1. XM_006507210.1.
    XP_006507274.1. XM_006507211.1.
    UniGenei Mm.275831.

    3D structure databases

    ProteinModelPortali P05064.
    SMRi P05064. Positions 5-344.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198067. 7 interactions.
    IntActi P05064. 20 interactions.
    MINTi MINT-1581489.

    PTM databases

    PhosphoSitei P05064.

    2D gel databases

    COMPLUYEAST-2DPAGE P05064.
    REPRODUCTION-2DPAGE IPI00221402.
    P05064.
    SWISS-2DPAGE P05064.

    Proteomic databases

    MaxQBi P05064.
    PaxDbi P05064.
    PRIDEi P05064.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032934 ; ENSMUSP00000032934 ; ENSMUSG00000030695 .
    ENSMUST00000106348 ; ENSMUSP00000101955 ; ENSMUSG00000030695 .
    GeneIDi 11674.
    KEGGi mmu:11674.
    UCSCi uc009jsu.2. mouse.

    Organism-specific databases

    CTDi 226.
    MGIi MGI:87994. Aldoa.

    Phylogenomic databases

    eggNOGi COG3588.
    HOGENOMi HOG000220876.
    HOVERGENi HBG002386.
    InParanoidi P05064.
    KOi K01623.
    OrthoDBi EOG744T94.
    PhylomeDBi P05064.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .
    SABIO-RK P05064.

    Miscellaneous databases

    ChiTaRSi ALDOA. mouse.
    NextBioi 279303.
    PROi P05064.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P05064.
    Bgeei P05064.
    CleanExi MM_ALDOA.
    Genevestigatori P05064.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    PROSITEi PS00158. ALDOLASE_CLASS_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: 129.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Nonconservative utilization of aldolase A alternative promoters."
      Stauffer J.K., Colbert M.C., Ciejek-Baez E.
      J. Biol. Chem. 265:11773-11782(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-266 AND 295-364.
    4. Lubec G., Kang S.U., Klug S., Yang J.W., Zigmond M., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-22; 29-57; 61-109; 112-134; 154-258; 260-312 AND 323-364, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    5. "Structure and expression of mouse aldolase genes. Brain-specific aldolase C amino acid sequence is closely related to aldolase A."
      Paolella G., Buono P., Mancini P., Izzo P., Salvatore F.
      Eur. J. Biochem. 156:229-235(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 99-355.

    Entry informationi

    Entry nameiALDOA_MOUSE
    AccessioniPrimary (citable) accession number: P05064
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 147 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3