P05060 (SCG1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Secretogranin-1

Alternative name(s):
Chromogranin-B
Short name=CgB
Secretogranin I
Short name=SgI

Cleaved into the following 2 chains:

1. GAWK peptide
2. CCB peptide

Gene names

Name:	CHGB
Synonyms:	SCG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Subcellular location	Secreted. Note: Neuroendocrine and endocrine secretory granules.
Tissue specificity	Expressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver. Ref.1
Post-translational modification	Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues By similarity. O-glycosylated. Ref.14
Sequence similarities	Belongs to the chromogranin/secretogranin protein family.
Sequence caution	The sequence BAD92949.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Acetylation Cleavage on pair of basic residues Disulfide bond Glycoprotein Phosphoprotein Sulfation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell secretory granule Inferred from electronic annotation. Source: Compara
Molecular_function	hormone activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SMAD2	Q15796	2	EBI-712619,EBI-1040141

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.1 Ref.8
	Chain	21 – 677	657	Secretogranin-1		PRO_0000005438
	Peptide	440 – 513	74	GAWK peptide Ref.10		PRO_0000005439
	Peptide	617 – 673	57	CCB peptide Ref.11		PRO_0000005440
Regions
	Region	116 – 120	5	O-glycosylated at one site
	Compositional bias	163 – 171	9	Poly-Glu
Amino acid modifications
	Modified residue	79	1	Phosphothreonine Probable
	Modified residue	99	1	Phosphoserine Potential
	Modified residue	100	1	Phosphoserine Potential
	Modified residue	149	1	Phosphoserine Ref.12 Ref.13
	Modified residue	153	1	N6-acetyllysine By similarity
	Modified residue	183	1	Phosphoserine By similarity
	Modified residue	259	1	Phosphoserine Ref.13
	Modified residue	263	1	Phosphoserine Ref.13
	Modified residue	293	1	Phosphoserine Potential
	Modified residue	294	1	Phosphoserine Potential
	Modified residue	311	1	Phosphoserine Ref.13
	Modified residue	335	1	Phosphoserine Ref.13
	Modified residue	341	1	Sulfotyrosine By similarity
	Modified residue	377	1	Phosphoserine By similarity
	Modified residue	380	1	Phosphoserine
	Modified residue	405	1	Phosphoserine Ref.12 Ref.13
	Modified residue	471	1	Sulfotyrosine Potential
	Modified residue	474	1	Sulfotyrosine Probable
	Modified residue	533	1	Phosphoserine Potential
	Modified residue	534	1	Phosphoserine Potential
	Modified residue	566	1	Sulfotyrosine Probable
	Modified residue	568	1	Sulfotyrosine Potential
	Modified residue	617	1	Phosphoserine Ref.13
	Modified residue	624	1	Sulfotyrosine By similarity
	Modified residue	626	1	Phosphoserine By similarity
	Modified residue	631	1	Phosphoserine By similarity
	Disulfide bond	36 ↔ 57
Natural variations
	Natural variant	93	1	S → T. Ref.2 Ref.4 Corresponds to variant rs6085324 [ dbSNP | Ensembl ].		VAR_043578
	Natural variant	117	1	K → N. Corresponds to variant rs236150 [ dbSNP | Ensembl ].		VAR_024414
	Natural variant	145	1	D → N. Corresponds to variant rs6133278 [ dbSNP | Ensembl ].		VAR_028235
	Natural variant	178	1	R → Q. Ref.2 Ref.4 Corresponds to variant rs910122 [ dbSNP | Ensembl ].		VAR_020287
	Natural variant	200	1	N → H. Corresponds to variant rs881118 [ dbSNP | Ensembl ].		VAR_028236
	Natural variant	232	1	R → Q. Corresponds to variant rs6139873 [ dbSNP | Ensembl ].		VAR_028237
	Natural variant	243	1	T → A. Ref.1 Ref.2 Ref.3 Ref.4 Ref.6 Ref.7 Corresponds to variant rs236151 [ dbSNP | Ensembl ].		VAR_024415
	Natural variant	353	1	A → G. Ref.2 Ref.4 Corresponds to variant rs236152 [ dbSNP | Ensembl ].		VAR_024416
	Natural variant	413	1	P → L. Corresponds to variant rs742710 [ dbSNP | Ensembl ].		VAR_028238
	Natural variant	417	1	R → H. Ref.2 Ref.4 Corresponds to variant rs742711 [ dbSNP | Ensembl ].		VAR_022012
Experimental info
	Sequence conflict	24	1	D → E in BAF82075. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05060 [UniParc]. Last modified November 2, 2010. Version 2. Checksum: BFDC97F7B21245B3	FASTA	677	78,276
	10 20 30 40 50 60 MQPTLLLSLL GAVGLAAVNS MPVDNRNHNE GMVTRCIIEV LSNALSKSSA PPITPECRQV 70 80 90 100 110 120 LKTSRKDVKD KETTENENTK FEVRLLRDPA DASEAHESSS RGEAGAPGEE DIQGPTKADT 130 140 150 160 170 180 EKWAEGGGHS RERADEPQWS LYPSDSQVSE EVKTRHSEKS QREDEEEEEG ENYQKGERGE 190 200 210 220 230 240 DSSEEKHLEE PGETQNAFLN ERKQASAIKK EELVARSETH AAGHSQEKTH SREKSSQESG 250 260 270 280 290 300 EETGSQENHP QESKGQPRSQ EESEEGEEDA TSEVDKRRTR PRHHHGRSRP DRSSQGGSLP 310 320 330 340 350 360 SEEKGHPQEE SEESNVSMAS LGEKRDHHST HYRASEEEPE YGEEIKGYPG VQAPEDLEWE 370 380 390 400 410 420 RYRGRGSEEY RAPRPQSEES WDEEDKRNYP SLELDKMAHG YGEESEEERG LEPGKGRHHR 430 440 450 460 470 480 GRGGEPRAYF MSDTREEKRF LGEGHHRVQE NQMDKARRHP QGAWKELDRN YLNYGEEGAP 490 500 510 520 530 540 GKWQQQGDLQ DTKENREEAR FQDKQYSSHH TAEKRKRLGE LFNPYYDPLQ WKSSHFERRD 550 560 570 580 590 600 NMNDNFLEGE EENELTLNEK NFFPEYNYDW WEKKPFSEDV NWGYEKRNLA RVPKLDLKRQ 610 620 630 640 650 660 YDRVAQLDQL LHYRKKSAEF PDFYDSEEPV STHQEAENEK DRADQTVLTE DEKKELENLA 670 AMDLELQKIA EKFSQRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of human secretogranin I (chromogranin B): comparison with chromogranin A reveals homologous terminal domains and a large intervening variable region." Benedum U.M., Lamouroux A., Konecki D.S., Hille A., Baeuerle P.A., Frank R., Lottspeich F., Mallet J., Huttner W.B. EMBO J. 6:1203-1211(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-34; 85-93; 334-340 AND 347-355, TISSUE SPECIFICITY, VARIANT ALA-243. Tissue: Adrenal medulla.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS THR-93; GLN-178; ALA-243; GLY-353 AND HIS-417. Tissue: Adrenal gland.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-243.
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS THR-93; GLN-178; ALA-243; GLY-353 AND HIS-417. Tissue: Brain.
[5]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ALA-243.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-243. Tissue: Lung.
[8]	"Chromogranin B: isolation from pheochromocytoma, N-terminal sequence, tissue distribution and secretory vesicle processing." Gill B.M., Barbosa J.A., Dinh T.Q., Garrod S., O'Connor D.T. Regul. Pept. 33:223-235(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-48.
[9]	"Identification of a new chromogranin B fragment (314-365) in endocrine tumors." Woussen-Colle M.-C., Gourlet P., Vandermeers A., Vandermeers-Piret M.-C., D'Haens J., Velkeniers B., Robberecht P. Peptides 16:231-236(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 334-385.
[10]	"GAWK, a novel human pituitary polypeptide: isolation, immunocytochemical localization and complete amino acid sequence." Benjannet S., Leduc R., Lazure C., Seidah N.G., Marcinkiewicz M., Chretien M. Biochem. Biophys. Res. Commun. 126:602-609(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 440-513. Tissue: Pituitary.
[11]	"Chromogranin B (secretogranin I), a putative precursor of two novel pituitary peptides through processing at paired basic residues." Benjannet S., Leduc R., Adrouche N., Falgueyret J.P., Marcinkiewicz M., Seidah N.G., Mbikay M., Lazure C., Chretien M. FEBS Lett. 224:142-148(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 617-673. Tissue: Pituitary.
[12]	"Identification and characterization of phosphorylated proteins in the human pituitary." Giorgianni F., Beranova-Giorgianni S., Desiderio D.M. Proteomics 4:587-598(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-149 AND SER-405. Tissue: Pituitary.
[13]	"Phosphoproteomic analysis of the human pituitary." Beranova-Giorgianni S., Zhao Y., Desiderio D.M., Giorgianni F. Pituitary 9:109-120(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149; SER-259; SER-263; SER-311; SER-335; SER-405 AND SER-617, MASS SPECTROMETRY. Tissue: Pituitary.
[14]	"LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins." Halim A., Ruetschi U., Larson G., Nilsson J. J. Proteome Res. 12:573-584(2013) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00064 mRNA. Translation: CAA68271.1. AK289386 mRNA. Translation: BAF82075.1. CR456726 mRNA. Translation: CAG33007.1. AB209712 mRNA. Translation: BAD92949.1. Different initiation. AL035461 Genomic DNA. Translation: CAB55272.1. AL035461 Genomic DNA. Translation: CAC34360.1. CH471133 Genomic DNA. Translation: EAX10410.1. BC000375 mRNA. Translation: AAH00375.1.
IPI	IPI00006601.
PIR	CNHUB. A29264.
RefSeq	NP_001810.2. NM_001819.2.
UniGene	Hs.516874.
3D structure databases
ProteinModelPortal	P05060.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05060. 24 interactions.
MINT	MINT-1414600.
STRING	9606.ENSP00000368244.
PTM databases
PhosphoSite	P05060.
Polymorphism databases
DMDM	311033509.
Proteomic databases
PaxDb	P05060.
PeptideAtlas	P05060.
PRIDE	P05060.
Protocols and materials databases
DNASU	1114.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000378961; ENSP00000368244; ENSG00000089199. ENST00000455042; ENSP00000416643; ENSG00000089199.
GeneID	1114.
KEGG	hsa:1114.
UCSC	uc002wmg.3. human.
Organism-specific databases
CTD	1114.
GeneCards	GC20P005891.
H-InvDB	HIX0015625.
HGNC	HGNC:1930. CHGB.
HPA	CAB009403. HPA008759. HPA012602.
MIM	118920. gene.
neXtProt	NX_P05060.
Orphanet	803. Amyotrophic lateral sclerosis.
PharmGKB	PA26462.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG39839.
HOVERGEN	HBG057317.
InParanoid	P05060.
OMA	NWGYEKR.
OrthoDB	EOG4Z0B5C.
PhylomeDB	P05060.
Gene expression databases
Bgee	P05060.
CleanEx	HS_CHGB.
Genevestigator	P05060.
GermOnline	ENSG00000089199. Homo sapiens.
Family and domain databases
InterPro	IPR001819. Chromogranin_AB. IPR018054. Chromogranin_CS. IPR001990. Granin. [Graphical view]
PANTHER	PTHR10583. PTHR10583. 1 hit.
Pfam	PF01271. Granin. 1 hit. [Graphical view]
PRINTS	PR00659. CHROMOGRANIN.
PROSITE	PS00422. GRANINS_1. 1 hit. PS00423. GRANINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CHGB. human.
GenomeRNAi	1114.
NextBio	4622.
SOURCE	Search...

Entry information

Entry name

SCG1_HUMAN

Accession

Primary (citable) accession number: P05060
Secondary accession number(s): A8K021 , Q59EU9, Q6IBS6, Q9BQV6, Q9UC25, Q9UJA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 141 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.