Reviewed, UniProtKB/Swiss-Prot P05060 (SCG1_HUMAN)

Names and origin

Protein names

Recommended name:
    Secretogranin-1
Alternative name(s):
    Secretogranin I
      Short name=SgI
    Chromogranin-B
      Short name=CgB
Cleaved into the following 2 chains:
    1- Recommended name:
            GAWK peptide
    2- Recommended name:
            CCB peptide

Gene names

Name:	CHGB
Synonyms:	SCG1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	677 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Subcellular location	Secreted. Note: Neuroendocrine and endocrine secretory granules.
Tissue specificity	Expressed in the adrenal medulla, and in pheochromocytoma. Not expressed in liver. Ref.1
Post-translational modification	Extensively processed by limited proteolysis at conserved basic residues. Alternative processing are seen in different tissues By similarity.
Sequence similarities	Belongs to the chromogranin/secretogranin protein family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
PTM	Acetylation Cleavage on pair of basic residues Disulfide bond Glycoprotein Phosphoprotein Sulfation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hormone activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.1 Ref.8
	Chain	21 – 677	657	Secretogranin-1		PRO_0000005438
	Peptide	440 – 513	74	GAWK peptide		PRO_0000005439
	Peptide	617 – 676	60	CCB peptide		PRO_0000005440
Regions
	Compositional bias	163 – 171	9	Poly-Glu
Amino acid modifications
	Modified residue	79	1	Phosphothreonine Probable
	Modified residue	99	1	Phosphoserine Potential
	Modified residue	100	1	Phosphoserine Potential
	Modified residue	149	1	Phosphoserine Ref.12 Ref.13
	Modified residue	153	1	N6-acetyllysine By similarity
	Modified residue	182	1	Phosphoserine Ref.13
	Modified residue	183	1	Phosphoserine Ref.13
	Modified residue	259	1	Phosphoserine Ref.13
	Modified residue	263	1	Phosphoserine Ref.13
	Modified residue	293	1	Phosphoserine Potential
	Modified residue	294	1	Phosphoserine Potential
	Modified residue	311	1	Phosphoserine Ref.13
	Modified residue	335	1	Phosphoserine Ref.13
	Modified residue	341	1	Sulfotyrosine By similarity
	Modified residue	377	1	Phosphoserine Ref.13
	Modified residue	380	1	Phosphoserine
	Modified residue	405	1	Phosphoserine Potential
	Modified residue	471	1	Sulfotyrosine Potential
	Modified residue	474	1	Sulfotyrosine Probable
	Modified residue	533	1	Phosphoserine Potential
	Modified residue	534	1	Phosphoserine Potential
	Modified residue	566	1	Sulfotyrosine Probable
	Modified residue	568	1	Sulfotyrosine Potential
	Modified residue	617	1	Phosphoserine Ref.13
	Modified residue	626	1	Phosphoserine Ref.13
	Modified residue	631	1	Phosphoserine By similarity
	Glycosylation	116	1	O-linked (GalNAc...) Probable
	Disulfide bond	36 ↔ 57
Natural variations
	Natural variant	93	1	S → T: dbSNP rs6085324. Ref.2 Ref.4		VAR_043578
	Natural variant	117	1	K → N: dbSNP rs236150.		VAR_024414
	Natural variant	145	1	D → N: dbSNP rs6133278.		VAR_028235
	Natural variant	178	1	R → Q: dbSNP rs910122. Ref.2 Ref.4		VAR_020287
	Natural variant	200	1	N → H: dbSNP rs881118.		VAR_028236
	Natural variant	232	1	R → Q: dbSNP rs6139873.		VAR_028237
	Natural variant	243	1	A → T: dbSNP rs236151. Ref.5		VAR_024415
	Natural variant	353	1	A → G: dbSNP rs236152. Ref.2 Ref.4		VAR_024416
	Natural variant	413	1	P → L: dbSNP rs742710.		VAR_028238
	Natural variant	417	1	R → H: dbSNP rs742711. Ref.2 Ref.4		VAR_022012
Experimental info
	Sequence conflict	24	1	D → E in BAF82075. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05060-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 1BD4E122B1C59B11	FASTA	677	78,246
	10 20 30 40 50 60 MQPTLLLSLL GAVGLAAVNS MPVDNRNHNE GMVTRCIIEV LSNALSKSSA PPITPECRQV 70 80 90 100 110 120 LKTSRKDVKD KETTENENTK FEVRLLRDPA DASEAHESSS RGEAGAPGEE DIQGPTKADT 130 140 150 160 170 180 EKWAEGGGHS RERADEPQWS LYPSDSQVSE EVKTRHSEKS QREDEEEEEG ENYQKGERGE 190 200 210 220 230 240 DSSEEKHLEE PGETQNAFLN ERKQASAIKK EELVARSETH AAGHSQEKTH SREKSSQESG 250 260 270 280 290 300 EEAGSQENHP QESKGQPRSQ EESEEGEEDA TSEVDKRRTR PRHHHGRSRP DRSSQGGSLP 310 320 330 340 350 360 SEEKGHPQEE SEESNVSMAS LGEKRDHHST HYRASEEEPE YGEEIKGYPG VQAPEDLEWE 370 380 390 400 410 420 RYRGRGSEEY RAPRPQSEES WDEEDKRNYP SLELDKMAHG YGEESEEERG LEPGKGRHHR 430 440 450 460 470 480 GRGGEPRAYF MSDTREEKRF LGEGHHRVQE NQMDKARRHP QGAWKELDRN YLNYGEEGAP 490 500 510 520 530 540 GKWQQQGDLQ DTKENREEAR FQDKQYSSHH TAEKRKRLGE LFNPYYDPLQ WKSSHFERRD 550 560 570 580 590 600 NMNDNFLEGE EENELTLNEK NFFPEYNYDW WEKKPFSEDV NWGYEKRNLA RVPKLDLKRQ 610 620 630 640 650 660 YDRVAQLDQL LHYRKKSAEF PDFYDSEEPV STHQEAENEK DRADQTVLTE DEKKELENLA 670 AMDLELQKIA EKFSQRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of human secretogranin I (chromogranin B): comparison with chromogranin A reveals homologous terminal domains and a large intervening variable region." Benedum U.M., Lamouroux A., Konecki D.S., Hille A., Baeuerle P.A., Frank R., Lottspeich F., Mallet J., Huttner W.B. EMBO J. 6:1203-1211(1987) [PubMed: 3608978] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-34; 85-93; 334-340 AND 347-355, TISSUE SPECIFICITY. Tissue: Adrenal medulla.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS THR-93; GLN-178; GLY-353 AND HIS-417. Tissue: Adrenal gland.
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS THR-93; GLN-178; GLY-353 AND HIS-417. Tissue: Brain.
[5]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed: 11780052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-243.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Chromogranin B: isolation from pheochromocytoma, N-terminal sequence, tissue distribution and secretory vesicle processing." Gill B.M., Barbosa J.A., Dinh T.Q., Garrod S., O'Connor D.T. Regul. Pept. 33:223-235(1991) [PubMed: 1882087] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-48.
[9]	"Identification of a new chromogranin B fragment (314-365) in endocrine tumors." Woussen-Colle M.-C., Gourlet P., Vandermeers A., Vandermeers-Piret M.-C., D'Haens J., Velkeniers B., Robberecht P. Peptides 16:231-236(1995) [PubMed: 7784254] [Abstract] Cited for: PROTEIN SEQUENCE OF 334-385.
[10]	"GAWK, a novel human pituitary polypeptide: isolation, immunocytochemical localization and complete amino acid sequence." Benjannet S., Leduc R., Lazure C., Seidah N.G., Marcinkiewicz M., Chretien M. Biochem. Biophys. Res. Commun. 126:602-609(1985) [PubMed: 3970711] [Abstract] Cited for: PROTEIN SEQUENCE OF 440-513. Tissue: Pituitary.
[11]	"Chromogranin B (secretogranin I), a putative precursor of two novel pituitary peptides through processing at paired basic residues." Benjannet S., Leduc R., Adrouche N., Falgueyret J.P., Marcinkiewicz M., Seidah N.G., Mbikay M., Lazure C., Chretien M. FEBS Lett. 224:142-148(1987) [PubMed: 3678488] [Abstract] Cited for: PROTEIN SEQUENCE OF 617-676. Tissue: Pituitary.
[12]	"Identification and characterization of phosphorylated proteins in the human pituitary." Giorgianni F., Beranova-Giorgianni S., Desiderio D.M. Proteomics 4:587-598(2004) [PubMed: 14997482] [Abstract] Cited for: PHOSPHORYLATION AT SER-149 AND SER-405. Tissue: Pituitary.
[13]	"Phosphoproteomic analysis of the human pituitary." Beranova-Giorgianni S., Zhao Y., Desiderio D.M., Giorgianni F. Pituitary 9:109-120(2006) [PubMed: 16807684] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-149; SER-182; SER-183; SER-259; SER-263; SER-311; SER-335; SER-377; SER-405; SER-617 AND SER-626, MASS SPECTROMETRY. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00064 mRNA. Translation: CAA68271.1. AK289386 mRNA. Translation: BAF82075.1. CR456726 mRNA. Translation: CAG33007.1. AB209712 mRNA. Translation: BAD92949.1. Different initiation. AL035461 Genomic DNA. Translation: CAB55272.1. AL035461 Genomic DNA. Translation: CAC34360.1. CH471133 Genomic DNA. Translation: EAX10410.1. BC000375 mRNA. Translation: AAH00375.1.
IPI	IPI00006601.
PIR	CNHUB. A29264.
RefSeq	NP_001810.2.
UniGene	Hs.516874
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P05060. 23 interactions.
PTM databases
PhosphoSite	P05060.
Proteomic databases
PeptideAtlas	P05060.
PRIDE	P05060.
Genome annotation databases
Ensembl	ENSG00000089199. Homo sapiens. [Contig view]
GeneID	1114.
KEGG	hsa:1114.
UCSC	uc002wmg.1. human.
Organism-specific databases
GeneCards	GC20P005840.
H-InvDB	HIX0015625.
HGNC	HGNC:1930. CHGB.
HPA	CAB009403. HPA008759. HPA012602.
MIM	118920. gene.
PharmGKB	PA26462.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	P05060.
Gene expression databases
ArrayExpress	P05060.
Bgee	P05060.
CleanEx	HS_CHGB.
GermOnline	ENSG00000089199. Homo sapiens.
Family and domain databases
InterPro	IPR001819. Chromogranin_AB. IPR018054. Chromogranin_CS. IPR001990. Granin. [Graphical view]
PANTHER	PTHR10583. Chromogranin_AB. 1 hit.
Pfam	PF01271. Granin. 1 hit. [Graphical view]
PRINTS	PR00659. CHROMOGRANIN.
PROSITE	PS00422. GRANINS_1. 1 hit. PS00423. GRANINS_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	4622.
SOURCE	Search...

Entry information

Entry name

SCG1_HUMAN

Accession

Primary (citable) accession number: P05060
Secondary accession number(s): A8K021 , Q59EU9, Q6IBS6, Q9BQV6, Q9UC25, Q9UJA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	July 7, 2009
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)