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Protein

Kanamycin nucleotidyltransferase

Gene

knt

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Inactivates the antibiotic kanamycin by catalyzing the transfer of a nucleotidyl group from nucleoside triphosphates such as ATP to the 4'-hydroxyl group of the aminoglycoside.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Kanamycin nucleotidyltransferase (EC:2.7.7.-)
Short name:
Neo(R)
Gene namesi
Name:knt
Synonyms:kan
Encoded oniPlasmid pUB1100 Publication
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Pathology & Biotechi

Chemistry

DrugBankiDB01172. Kanamycin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 253253Kanamycin nucleotidyltransferasePRO_0000068568Add
BLAST

Proteomic databases

PaxDbiP05057.
PRIDEiP05057.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi158878.SAV0035.

Structurei

Secondary structure

1
253
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 2719Combined sources
Helixi28 – 303Combined sources
Beta strandi31 – 377Combined sources
Helixi38 – 425Combined sources
Beta strandi51 – 599Combined sources
Beta strandi63 – 697Combined sources
Beta strandi74 – 818Combined sources
Helixi82 – 898Combined sources
Helixi96 – 994Combined sources
Helixi100 – 1034Combined sources
Beta strandi107 – 1148Combined sources
Helixi115 – 12410Combined sources
Helixi128 – 14114Combined sources
Helixi143 – 15614Combined sources
Helixi159 – 1613Combined sources
Helixi162 – 18120Combined sources
Beta strandi186 – 1905Combined sources
Helixi191 – 1955Combined sources
Beta strandi198 – 2003Combined sources
Helixi205 – 21410Combined sources
Helixi220 – 24122Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KANX-ray3.00A/B1-253[»]
1KNYX-ray2.50A/B1-253[»]
ProteinModelPortaliP05057.
SMRiP05057. Positions 1-253.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05057.

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108S3A. Bacteria.
ENOG4111GRA. LUCA.
KOiK17882.

Family and domain databases

InterProiIPR012481. KNTase_C.
[Graphical view]
PfamiPF07827. KNTase_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05057-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGPIIMTRE ERMKIVHEIK ERILDKYGDD VKAIGVYGSL GRQTDGPYSD
60 70 80 90 100
IEMMCVMSTE EAEFSHEWTT GEWKVEVNFD SEEILLDYAS QVESDWPLTH
110 120 130 140 150
GQFFSILPIY DSGGYLEKVY QTAKSVEAQT FHDAICALIV EELFEYAGKW
160 170 180 190 200
RNIRVQGPTT FLPSLTVQVA MAGAMLIGLH HRICYTTSAS VLTEAVKQSD
210 220 230 240 250
LPSGYDHLCQ FVMSGQLSDS EKLLESLENF WNGIQEWTER HGYIVDVSKR

IPF
Length:253
Mass (Da):28,798
Last modified:August 13, 1987 - v1
Checksum:i9CB3D60E72A45DC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03408 Genomic DNA. Translation: CAA27142.1.
M37273 Genomic DNA. Translation: AAA98214.1.
M19465 Genomic DNA. Translation: AAA88361.1.
RefSeqiNP_040433.1. NC_001384.1.
WP_001014230.1. NC_013342.1.
YP_006937662.1. NC_013320.1.
YP_006938491.1. NC_013342.1.

Genome annotation databases

GeneIDi13874812.
13875662.
3284089.
KEGGiag:AAA88361.
pg:13874812.
pg:13875662.
pg:3284089.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03408 Genomic DNA. Translation: CAA27142.1.
M37273 Genomic DNA. Translation: AAA98214.1.
M19465 Genomic DNA. Translation: AAA88361.1.
RefSeqiNP_040433.1. NC_001384.1.
WP_001014230.1. NC_013342.1.
YP_006937662.1. NC_013320.1.
YP_006938491.1. NC_013342.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KANX-ray3.00A/B1-253[»]
1KNYX-ray2.50A/B1-253[»]
ProteinModelPortaliP05057.
SMRiP05057. Positions 1-253.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi158878.SAV0035.

Chemistry

DrugBankiDB01172. Kanamycin.

Proteomic databases

PaxDbiP05057.
PRIDEiP05057.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13874812.
13875662.
3284089.
KEGGiag:AAA88361.
pg:13874812.
pg:13875662.
pg:3284089.

Phylogenomic databases

eggNOGiENOG4108S3A. Bacteria.
ENOG4111GRA. LUCA.
KOiK17882.

Miscellaneous databases

EvolutionaryTraceiP05057.

Family and domain databases

InterProiIPR012481. KNTase_C.
[Graphical view]
PfamiPF07827. KNTase_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants."
    Mueller R.E., Ano T., Imanaka T., Aiba S.
    Mol. Gen. Genet. 202:169-171(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Enzymatic and nucleotide sequence studies of a kanamycin-inactivating enzyme encoded by a plasmid from thermophilic bacilli in comparison with that encoded by plasmid pUB110."
    Matsumura M., Katakura Y., Imanaka T., Aiba S.
    J. Bacteriol. 160:413-420(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7.
  3. "Nucleotide sequence and physical map of kanamycin-resistant plasmid pUB110 from Staphylococcus aureus."
    Bashkirov V.I., Mil'Shina N.V., Prozorov A.A.
    Genetika 22:1081-1092(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation."
    McKenzie T., Hoshino T., Tanaka T., Sueoka N.
    Plasmid 15:93-103(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Correction. A revision of the nucleotide sequence and functional map of pUB110."
    McKenzie T., Hoshino T., Tanaka T., Sueoka N.
    Plasmid 17:83-85(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  6. "Molecular structure of kanamycin nucleotidyltransferase determined to 3.0-A resolution."
    Sakon J., Liao H.H., Kanikula A.M., Benning M.M., Rayment I., Holden H.M.
    Biochemistry 32:11977-11984(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
  7. "Structural investigation of the antibiotic and ATP-binding sites in kanamycin nucleotidyltransferase."
    Pedersen L.C., Benning M.M., Holden H.M.
    Biochemistry 34:13305-13311(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiKANU_STAAU
AccessioniPrimary (citable) accession number: P05057
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.