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Protein

HTH-type transcriptional regulator AppY

Gene

appY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Induces the synthesis of acid phosphatase (AppA) and several other polypeptides (such as AppBC) during the deceleration phase of growth. It also acts as a transcriptional repressor for one group of proteins that are synthesized preferentially in exponential growth and for one group synthesized only in the stationary phase. Also involved in the stabilization of the sigma stress factor RpoS during stress conditions.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi155 – 17420H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cellular response to phosphate starvation Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00967.
ECOL316407:JW0553-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator AppY
Alternative name(s):
M5 polypeptide
Gene namesi
Name:appY
Ordered Locus Names:b0564, JW0553
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10050. appY.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249HTH-type transcriptional regulator AppYPRO_0000194497Add
BLAST

Proteomic databases

PaxDbiP05052.
PRIDEiP05052.

Interactioni

Protein-protein interaction databases

BioGridi4263522. 5 interactions.
IntActiP05052. 26 interactions.
STRINGi511145.b0564.

Structurei

3D structure databases

ProteinModelPortaliP05052.
SMRiP05052. Positions 133-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108MIW. Bacteria.
COG2207. LUCA.
HOGENOMiHOG000120708.
InParanoidiP05052.
KOiK07780.
OMAiKRERYPA.
OrthoDBiEOG63C0R5.
PhylomeDBiP05052.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYVCSVVFI CQSFDLIINR RVISFKKNSL FIVSDKIRRE LPVCPSKLRI
60 70 80 90 100
VDIDKKTCLS FFIDVNNELP GKFTLDKNGY IAEEEPPLSL VFSLFEGIKI
110 120 130 140 150
ADSHSLWLKE RLCISLLAMF KKRESVNSFI LTNINTFTCK ITGIISFNIE
160 170 180 190 200
RQWHLKDIAE LIYTSESLIK KRLRDEGTSF TEILRDTRMR YAKKLITSNS
210 220 230 240
YSINVVAQKC GYNSTSYFIC AFKDYYGVTP SHYFEKIIGV TDGINKTID
Length:249
Mass (Da):28,763
Last modified:November 1, 1997 - v3
Checksum:i3985FF0ECCF03D8A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 316Missing (PubMed:2537825).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00138 Genomic DNA. Translation: CAA68331.1.
M24530 Genomic DNA. Translation: AAA23454.1.
U82598 Genomic DNA. Translation: AAB40760.1.
U00096 Genomic DNA. Translation: AAC73665.1.
AP009048 Genomic DNA. Translation: BAE76339.1.
PIRiA94498. BVECM5.
RefSeqiNP_415096.1. NC_000913.3.
WP_000386784.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73665; AAC73665; b0564.
BAE76339; BAE76339; BAE76339.
GeneIDi948797.
KEGGiecj:JW0553.
eco:b0564.
PATRICi32116294. VBIEscCol129921_0587.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00138 Genomic DNA. Translation: CAA68331.1.
M24530 Genomic DNA. Translation: AAA23454.1.
U82598 Genomic DNA. Translation: AAB40760.1.
U00096 Genomic DNA. Translation: AAC73665.1.
AP009048 Genomic DNA. Translation: BAE76339.1.
PIRiA94498. BVECM5.
RefSeqiNP_415096.1. NC_000913.3.
WP_000386784.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP05052.
SMRiP05052. Positions 133-236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263522. 5 interactions.
IntActiP05052. 26 interactions.
STRINGi511145.b0564.

Proteomic databases

PaxDbiP05052.
PRIDEiP05052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73665; AAC73665; b0564.
BAE76339; BAE76339; BAE76339.
GeneIDi948797.
KEGGiecj:JW0553.
eco:b0564.
PATRICi32116294. VBIEscCol129921_0587.

Organism-specific databases

EchoBASEiEB0048.
EcoGeneiEG10050. appY.

Phylogenomic databases

eggNOGiENOG4108MIW. Bacteria.
COG2207. LUCA.
HOGENOMiHOG000120708.
InParanoidiP05052.
KOiK07780.
OMAiKRERYPA.
OrthoDBiEOG63C0R5.
PhylomeDBiP05052.

Enzyme and pathway databases

BioCyciEcoCyc:PD00967.
ECOL316407:JW0553-MONOMER.

Miscellaneous databases

PROiP05052.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence and deduced amino acid sequence of the M5 polypeptide gene of Escherichia coli."
    Kemp E.H., Minton N.P., Mann N.H.
    Nucleic Acids Res. 15:3924-3924(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / ATCC 35607 / JM83.
  2. "Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growth-phase-dependent gene expression in Escherichia coli."
    Atlung T., Nielsen A., Hansen F.G.
    J. Bacteriol. 171:1683-1691(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Role of the transcriptional activator AppY in regulation of the cyx appA operon of Escherichia coli by anaerobiosis, phosphate starvation, and growth phase."
    Atlung T., Brondsted L.
    J. Bacteriol. 176:5414-5422(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors."
    Bougdour A., Cunning C., Baptiste P.J., Elliott T., Gottesman S.
    Mol. Microbiol. 68:298-313(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN THE STABILIZATION OF RPOS.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiAPPY_ECOLI
AccessioniPrimary (citable) accession number: P05052
Secondary accession number(s): Q2MBL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded by the cryptic lambdoid prophage DLP12.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.