P05052 (APPY_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator AppY

Alternative name(s):
M5 polypeptide

Gene names

Name:	appY
Ordered Locus Names:	b0564, JW0553

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Induces the synthesis of acid phosphatase (AppA) and several other polypeptides (such as AppBC) during the deceleration phase of growth. It also acts as a transcriptional repressor for one group of proteins that are synthesized preferentially in exponential growth and for one group synthesized only in the stationary phase. Also involved in the stabilization of the sigma stress factor RpoS during stress conditions. Ref.7

Sequence similarities

Contains 1 HTH araC/xylS-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular response to phosphate starvation Inferred from direct assay. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	HTH-type transcriptional regulator AppY		PRO_0000194497
Regions
	DNA binding	155 – 174	20	H-T-H motif By similarity
Experimental info
	Sequence conflict	26 – 31	6	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P05052 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 3985FF0ECCF03D8A	FASTA	249	28,763
	10 20 30 40 50 60 MDYVCSVVFI CQSFDLIINR RVISFKKNSL FIVSDKIRRE LPVCPSKLRI VDIDKKTCLS 70 80 90 100 110 120 FFIDVNNELP GKFTLDKNGY IAEEEPPLSL VFSLFEGIKI ADSHSLWLKE RLCISLLAMF 130 140 150 160 170 180 KKRESVNSFI LTNINTFTCK ITGIISFNIE RQWHLKDIAE LIYTSESLIK KRLRDEGTSF 190 200 210 220 230 240 TEILRDTRMR YAKKLITSNS YSINVVAQKC GYNSTSYFIC AFKDYYGVTP SHYFEKIIGV TDGINKTID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence and deduced amino acid sequence of the M5 polypeptide gene of Escherichia coli." Kemp E.H., Minton N.P., Mann N.H. Nucleic Acids Res. 15:3924-3924(1987) [PubMed: 3295784] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / ATCC 35607 / JM83.
[2]	"Isolation, characterization, and nucleotide sequence of appY, a regulatory gene for growth-phase-dependent gene expression in Escherichia coli." Atlung T., Nielsen A., Hansen F.G. J. Bacteriol. 171:1683-1691(1989) [PubMed: 2537825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Role of the transcriptional activator AppY in regulation of the cyx appA operon of Escherichia coli by anaerobiosis, phosphate starvation, and growth phase." Atlung T., Brondsted L. J. Bacteriol. 176:5414-5422(1994) [PubMed: 8071219] [Abstract] Cited for: CHARACTERIZATION.
[7]	"Multiple pathways for regulation of sigmaS (RpoS) stability in Escherichia coli via the action of multiple anti-adaptors." Bougdour A., Cunning C., Baptiste P.J., Elliott T., Gottesman S. Mol. Microbiol. 68:298-313(2008) [PubMed: 18383615] [Abstract] Cited for: FUNCTION IN THE STABILIZATION OF RPOS. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00138 Genomic DNA. Translation: CAA68331.1. M24530 Genomic DNA. Translation: AAA23454.1. U82598 Genomic DNA. Translation: AAB40760.1. U00096 Genomic DNA. Translation: AAC73665.1. AP009048 Genomic DNA. Translation: BAE76339.1.
PIR	BVECM5. A94498.
RefSeq	NP_415096.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P05052.
SMR	P05052. Positions 135-238.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05052. 25 interactions.
Protein family/group databases
AraC-XylS	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003944; EBESCP00000003944; EBESCG00000003229. EBESCT00000014251; EBESCP00000013542; EBESCG00000013313.
GeneID	948797.
GenomeReviews	Gene locus JW0553 in contig AP009048_GR. Gene locus b0564 in contig U00096_GR.
KEGG	ecj:JW0553. eco:b0564.
PATRIC	32116294. VBIEscCol129921_0587.
Organism-specific databases
EchoBASE	EB0048.
EcoGene	EG10050. appY.
Phylogenomic databases
eggNOG	COG2207.
GeneTree	EBGT00050000008641.
HOGENOM	HBG467631.
OMA	ITIDTEN.
PhylomeDB	P05052.
ProtClustDB	CLSK880699.
Enzyme and pathway databases
BioCyc	EcoCyc:PD00967.
Gene expression databases
Genevestigator	P05052.
Family and domain databases
InterPro	IPR009057. Homeodomain-like. IPR012287. Homeodomain-rel. IPR018062. HTH_AraC-typ_sub_2. IPR018060. HTH_AraC_DNA-bd. IPR020449. Tscrpt_reg_HTH_AraC-type. [Graphical view]
Gene3D	G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.
KO	K07780.
PRINTS	PR00032. HTHARAC.
SMART	SM00342. HTH_ARAC. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00041. HTH_ARAC_FAMILY_1. 1 hit. PS01124. HTH_ARAC_FAMILY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APPY_ECOLI

Accession

Primary (citable) accession number: P05052
Secondary accession number(s): Q2MBL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 103 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program