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Protein

HTH-type transcriptional regulator AppY

Gene

appY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Induces the synthesis of acid phosphatase (AppA) and several other polypeptides (such as AppBC) during the deceleration phase of growth. It also acts as a transcriptional repressor for one group of proteins that are synthesized preferentially in exponential growth and for one group synthesized only in the stationary phase. Also involved in the stabilization of the sigma stress factor RpoS during stress conditions.1 Publication

Miscellaneous

Encoded by the cryptic lambdoid prophage DLP12.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi155 – 174H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • cellular response to phosphate starvation Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • response to X-ray Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:PD00967.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator AppY
Alternative name(s):
M5 polypeptide
Gene namesi
Name:appY
Ordered Locus Names:b0564, JW0553
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10050. appY.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944971 – 249HTH-type transcriptional regulator AppYAdd BLAST249

Proteomic databases

PaxDbiP05052.
PRIDEiP05052.

Interactioni

Protein-protein interaction databases

BioGridi4263522. 5 interactors.
IntActiP05052. 26 interactors.
STRINGi316385.ECDH10B_0630.

Structurei

3D structure databases

ProteinModelPortaliP05052.
SMRiP05052.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiENOG4108MIW. Bacteria.
COG2207. LUCA.
HOGENOMiHOG000120708.
InParanoidiP05052.
KOiK07780.
PhylomeDBiP05052.

Family and domain databases

InterProiView protein in InterPro
IPR009057. Homeobox-like_sf.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
PfamiView protein in Pfam
PF12833. HTH_18. 1 hit.
PRINTSiPR00032. HTHARAC.
SMARTiView protein in SMART
SM00342. HTH_ARAC. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P05052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYVCSVVFI CQSFDLIINR RVISFKKNSL FIVSDKIRRE LPVCPSKLRI
60 70 80 90 100
VDIDKKTCLS FFIDVNNELP GKFTLDKNGY IAEEEPPLSL VFSLFEGIKI
110 120 130 140 150
ADSHSLWLKE RLCISLLAMF KKRESVNSFI LTNINTFTCK ITGIISFNIE
160 170 180 190 200
RQWHLKDIAE LIYTSESLIK KRLRDEGTSF TEILRDTRMR YAKKLITSNS
210 220 230 240
YSINVVAQKC GYNSTSYFIC AFKDYYGVTP SHYFEKIIGV TDGINKTID
Length:249
Mass (Da):28,763
Last modified:November 1, 1997 - v3
Checksum:i3985FF0ECCF03D8A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26 – 31Missing (PubMed:2537825).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00138 Genomic DNA. Translation: CAA68331.1.
M24530 Genomic DNA. Translation: AAA23454.1.
U82598 Genomic DNA. Translation: AAB40760.1.
U00096 Genomic DNA. Translation: AAC73665.1.
AP009048 Genomic DNA. Translation: BAE76339.1.
PIRiA94498. BVECM5.
RefSeqiNP_415096.1. NC_000913.3.
WP_000386784.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73665; AAC73665; b0564.
BAE76339; BAE76339; BAE76339.
GeneIDi948797.
KEGGiecj:JW0553.
eco:b0564.
PATRICifig|511145.12.peg.587.

Similar proteinsi

Entry informationi

Entry nameiAPPY_ECOLI
AccessioniPrimary (citable) accession number: P05052
Secondary accession number(s): Q2MBL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1997
Last modified: November 22, 2017
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene