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P05045

- LEC1_VIGUC

UniProt

P05045 - LEC1_VIGUC

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Protein

Seed lectin subunit I

Gene
N/A
Organism
Vigna unguiculata subsp. cylindrica (Horse gram) (Dolichos biflorus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Metalloglycoprotein, containing Ca, Mg, Mn, and Zn and the carbohydrates galactose, glucosamine, mannose, and fucose. It agglutinates erythrocytes of blood group A1. Has a high preference for GalNAc over Gal.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei265 – 2662Cleavage

GO - Molecular functioni

  1. mannose binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin, Mannose-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Seed lectin subunit I
Short name:
SL
Cleaved into the following chain:
OrganismiVigna unguiculata subsp. cylindrica (Horse gram) (Dolichos biflorus)
Taxonomic identifieri3840 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Pathology & Biotechi

Protein family/group databases

Allergomei3011. Dol b Agglutinin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Add
BLAST
Chaini23 – 275253Seed lectin subunit IPRO_0000017611Add
BLAST
Chaini23 – 265243Seed lectin subunit IIPRO_0000017612Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Subunit II may arise from subunit I by proteolytic cleavage at the C-terminal end.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
275
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi24 – 329Combined sources
Helixi35 – 373Combined sources
Beta strandi38 – 425Combined sources
Beta strandi45 – 473Combined sources
Beta strandi50 – 523Combined sources
Beta strandi67 – 748Combined sources
Turni81 – 844Combined sources
Beta strandi88 – 969Combined sources
Beta strandi107 – 1159Combined sources
Helixi124 – 1263Combined sources
Turni127 – 1293Combined sources
Helixi137 – 1393Combined sources
Beta strandi142 – 1476Combined sources
Turni152 – 1543Combined sources
Beta strandi160 – 16910Combined sources
Beta strandi171 – 1755Combined sources
Beta strandi184 – 1918Combined sources
Turni192 – 1954Combined sources
Beta strandi196 – 2027Combined sources
Helixi204 – 2063Combined sources
Beta strandi209 – 2157Combined sources
Helixi218 – 2214Combined sources
Beta strandi224 – 23411Combined sources
Beta strandi245 – 25511Combined sources
Beta strandi257 – 2593Combined sources
Helixi266 – 2738Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BJQX-ray2.65A/B/C/D/E/F/G/H23-275[»]
1LU1X-ray2.60A23-275[»]
1LU2X-ray2.80A/B23-275[»]
ProteinModelPortaliP05045.
SMRiP05045. Positions 23-275.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05045.

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05045-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSTVSVVL SLFLLLLTQA NSANIQSFSF KNFNSPSFIL QGDATVSSGK
60 70 80 90 100
LQLTKVKENG IPTPSSLGRA FYSSPIQIYD KSTGAVASWA TSFTVKISAP
110 120 130 140 150
SKASFADGIA FALVPVGSEP RRNGGYLGVF DSDVYNNSAQ TVAVEFDTFS
160 170 180 190 200
NSGWDPSMKH IGIDVNSIKS IATVSWDLAN GENAEILITY NAATSLLVAS
210 220 230 240 250
LVHPSRRTSY ILSERVDITN ELPEYVSVGF SATTGLSEGY IETHDVLSWS
260 270
FASKLPDDST AEPLDLASYL VRNVL
Length:275
Mass (Da):29,406
Last modified:July 15, 1999 - v2
Checksum:iD313D73860661A83
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 677IPTPSSL → FPLRFPS in AAA33141. (PubMed:3584113)Curated
Sequence conflicti82 – 821S → F in AAA33141. (PubMed:3584113)Curated
Sequence conflicti149 – 1491F → L in AAA33141. (PubMed:3584113)Curated
Sequence conflicti199 – 1991A → V in AAA33141. (PubMed:3584113)Curated
Sequence conflicti227 – 2271S → G in AAA33141. (PubMed:3584113)Curated
Sequence conflicti254 – 2541K → R in AAA33141. (PubMed:3584113)Curated
Sequence conflicti268 – 2681S → R in AAA33141. (PubMed:3584113)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02721 mRNA. Translation: AAA33141.1.
M34270 Genomic DNA. Translation: AAA33143.1.
PIRiA29572.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02721 mRNA. Translation: AAA33141.1 .
M34270 Genomic DNA. Translation: AAA33143.1 .
PIRi A29572.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BJQ X-ray 2.65 A/B/C/D/E/F/G/H 23-275 [» ]
1LU1 X-ray 2.60 A 23-275 [» ]
1LU2 X-ray 2.80 A/B 23-275 [» ]
ProteinModelPortali P05045.
SMRi P05045. Positions 23-275.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3011. Dol b Agglutinin.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P05045.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view ]
Pfami PF00139. Lectin_legB. 1 hit.
[Graphical view ]
PIRSFi PIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the Dolichos biflorus seed lectin."
    Schnell D.J., Etzler M.E.
    J. Biol. Chem. 262:7220-7225(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Two lectin genes differentially expressed in Dolichos biflorus differ primarily by a 116-base pair sequence in their 5' flanking regions."
    Harada J.J., Spadoro-Tank J., Maxwell J.C., Schnell D.J., Etzler M.E.
    J. Biol. Chem. 265:4997-5001(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Carbohydrate binding, quaternary structure and a novel hydrophobic binding site in two legume lectin oligomers from Dolichos biflorus."
    Hamelryck T.W., Loris R., Bouckaert J., Dao-Thi M.-H., Strecker G., Imberty A., Fernandez E., Wyns L., Etzler M.E.
    J. Mol. Biol. 286:1161-1177(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).

Entry informationi

Entry nameiLEC1_VIGUC
AccessioniPrimary (citable) accession number: P05045
Secondary accession number(s): Q39666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: July 15, 1999
Last modified: November 26, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3