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Protein

Sorcin

Gene

SRI

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the sarcoplasmic reticulum in the heart. Modulates the activity of RYR2 calcium channels.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi83 – 94121Add
BLAST
Calcium bindingi113 – 124122Add
BLAST

GO - Molecular functioni

  • calcium ion binding Source: BHF-UCL
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorcin
Alternative name(s):
22 kDa protein
CP-22
V19
Gene namesi
Name:SRI
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

GO - Cellular componenti

  • membrane Source: BHF-UCL
  • sarcoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Sarcoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi99 – 991W → G: No significant effect. 1 Publication
Mutagenesisi105 – 1051W → G: Reduced affinity for calcium, resulting in reduced interaction with ANXA7 and strongly reduced modulation of RYR2 channel activity. 1 Publication
Mutagenesisi124 – 1241E → A: Strongly reduced modulation of RYR2 channel activity. 1 Publication
Mutagenesisi178 – 1781S → D: Increases calcium-sensitivity and promotes interaction with sarcoplasmic reticulum membranes. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198SorcinPRO_0000073724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated; partially.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP05044.

PTM databases

iPTMnetiP05044.

Expressioni

Tissue specificityi

Detected in myocardium.1 Publication

Interactioni

Subunit structurei

Homodimer. Interacts with GCA and RYR2 (By similarity). Interacts with ANXA7.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Anxa7Q070762EBI-10816377,EBI-10824194From a different organism.

Protein-protein interaction databases

IntActiP05044. 2 interactions.

Structurei

Secondary structure

1
198
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi36 – 427Combined sources
Helixi51 – 6111Combined sources
Helixi72 – 8211Combined sources
Helixi92 – 11221Combined sources
Beta strandi118 – 1203Combined sources
Helixi122 – 1309Combined sources
Turni131 – 1333Combined sources
Helixi138 – 14710Combined sources
Beta strandi153 – 1553Combined sources
Helixi156 – 17621Combined sources
Beta strandi182 – 1876Combined sources
Helixi188 – 1969Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GJYX-ray2.20A/B/C/D33-198[»]
ProteinModelPortaliP05044.
SMRiP05044. Positions 33-198.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05044.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 6436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini70 – 10334EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini100 – 13536EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini134 – 16936EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG004492.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05044-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYPGHPGAG GGYYPGGYGG APGGPSFPGQ TQDPLYGYFA SVAGQDGQID
60 70 80 90 100
ADELQRCLTQ SGIAGGYKPF NLETCRLMVS MLDRDMSGTM GFNEFKELWA
110 120 130 140 150
VLNGWRQHFI SFDSDRSGTV DPQELQKALT TMGFRLNPQT VNSIAKRYST
160 170 180 190
SGKITFDDYI ACCVKLRALT DSFRRRDSAQ QGMVNFSYDD FIQCVMTV
Length:198
Mass (Da):21,746
Last modified:August 13, 1987 - v1
Checksum:iDA2AF8A9707561C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04656 mRNA. Translation: CAA28354.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04656 mRNA. Translation: CAA28354.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GJYX-ray2.20A/B/C/D33-198[»]
ProteinModelPortaliP05044.
SMRiP05044. Positions 33-198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP05044. 2 interactions.

PTM databases

iPTMnetiP05044.

Proteomic databases

PRIDEiP05044.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG004492.

Miscellaneous databases

EvolutionaryTraceiP05044.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13405. EF-hand_6. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A 22-kd protein (sorcin/V19) encoded by an amplified gene in multidrug-resistant cells, is homologous to the calcium-binding light chain of calpain."
    van der Bliek A.M., Meyers M.B., Biedler J.L., Hes E., Borst P.
    EMBO J. 5:3201-3208(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. "Sorcin interacts with sarcoplasmic reticulum Ca(2+)-ATPase and modulates excitation-contraction coupling in the heart."
    Matsumoto T., Hisamatsu Y., Ohkusa T., Inoue N., Sato T., Suzuki S., Ikeda Y., Matsuzaki M.
    Basic Res. Cardiol. 100:250-262(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-178, MUTAGENESIS OF SER-178, TISSUE SPECIFICITY.
  3. "The W105G and W99G sorcin mutants demonstrate the role of the D helix in the Ca(2+)-dependent interaction with annexin VII and the cardiac ryanodine receptor."
    Colotti G., Zamparelli C., Verzili D., Mella M., Loughrey C.M., Smith G.L., Chiancone E.
    Biochemistry 45:12519-12529(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TRP-99; TRP-105 AND GLU-124, CALCIUM-BINDING, FUNCTION, INTERACTION WITH ANXA7, SUBUNIT.
  4. "The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein."
    Ilari A., Johnson K.A., Nastopoulos V., Verzili D., Zamparelli C., Colotti G., Tsernoglou D., Chiancone E.
    J. Mol. Biol. 317:447-458(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 33-198.

Entry informationi

Entry nameiSORCN_CRIGR
AccessioniPrimary (citable) accession number: P05044
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: January 20, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is encoded by an amplified gene in multidrug-resistant cells.
This protein has been shown to bind calcium with high affinity.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.