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Protein

Plasma membrane ATPase 1

Gene

PMA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Miscellaneous

The prion state [GAR+] is provoked by the interaction of the two proteins STD1 and PMA1. It involves a complex between a small fraction of the cellular complement of PMA1, and STD1, a much lower-abundance protein, and it is transmissible by non-Mendelian, cytoplasmic inheritance. [GAR+] makes cells resistant to the glucose-associated repression of alternative carbon sources. In contrast to other prion forms, [GAR+] cannot be cured by GdnHCl or by inactivation of the molecular chaperone HSP104.1 Publication
There are two plasma membrane ATPases in yeast. This is the major isoform.
Present with 1260000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3784-aspartylphosphate intermediateBy similarity1
Metal bindingi634MagnesiumBy similarity1
Metal bindingi638MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • proteasome storage granule assembly Source: SGD
  • proton export across plasma membrane Source: InterPro
  • proton transmembrane transport Source: SGD
  • regulation of pH Source: SGD
  • transmembrane transport Source: SGD

Keywordsi

Molecular functionHydrolase, Prion
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30531-MONOMER
BRENDAi3.6.3.6 984
SABIO-RKiP05030

Protein family/group databases

TCDBi3.A.3.3.6 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane ATPase 1 (EC:3.6.3.6)
Alternative name(s):
Proton pump 1
Gene namesi
Name:PMA1
Ordered Locus Names:YGL008C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL008C
SGDiS000002976 PMA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 115CytoplasmicSequence analysisAdd BLAST115
Transmembranei116 – 136Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini137 – 140ExtracellularSequence analysis4
Transmembranei141 – 160Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini161 – 291CytoplasmicSequence analysisAdd BLAST131
Transmembranei292 – 313Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini314 – 325ExtracellularSequence analysisAdd BLAST12
Transmembranei326 – 347Helical; Name=4Sequence analysisAdd BLAST22
Topological domaini348 – 719CytoplasmicSequence analysisAdd BLAST372
Transmembranei720 – 738Helical; Name=5Sequence analysisAdd BLAST19
Topological domaini739 – 754ExtracellularSequence analysisAdd BLAST16
Transmembranei755 – 774Helical; Name=6Sequence analysisAdd BLAST20
Topological domaini775 – 824CytoplasmicSequence analysisAdd BLAST50
Transmembranei825 – 845Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini846 – 857ExtracellularSequence analysisAdd BLAST12
Transmembranei858 – 874Helical; Name=8Sequence analysisAdd BLAST17
Topological domaini875 – 918CytoplasmicSequence analysisAdd BLAST44

Keywords - Cellular componenti

Amyloid, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi129E → L or Q: Normal activity. 1 Publication1
Mutagenesisi200D → N: Activity reduced to 23%. 1 Publication1
Mutagenesisi233E → Q: Activity reduced to 33%. 1 Publication1
Mutagenesisi271R → T: Normal activity. 1 Publication1
Mutagenesisi335P → A: Activity reduced to 53%. 1 Publication1
Mutagenesisi378D → E: Activity reduced to 67%. 1 Publication1
Mutagenesisi378D → N: Activity reduced to 73%. 1 Publication1
Mutagenesisi378D → T: Activity reduced to 49%. 1 Publication1
Mutagenesisi474K → Q: Activity reduced to 19%. 1 Publication1
Mutagenesisi534D → N: Activity reduced to 37%. 1 Publication1
Mutagenesisi560D → N: Activity reduced to 24%. 1 Publication1
Mutagenesisi638D → N: Activity reduced to 24%. 1 Publication1
Mutagenesisi848N → D: Normal activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462711 – 918Plasma membrane ATPase 1Add BLAST918

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei61PhosphoserineCombined sources1
Modified residuei175PhosphothreonineCombined sources1
Cross-linki252Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki555Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei911PhosphoserineCombined sources1
Modified residuei912PhosphothreonineCombined sources1
Modified residuei918PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated on multiple Ser and Thr residues.

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP05030
PaxDbiP05030
PRIDEiP05030

PTM databases

CarbonylDBiP05030
iPTMnetiP05030

Interactioni

Protein-protein interaction databases

BioGridi33238, 296 interactors
DIPiDIP-2537N
IntActiP05030, 22 interactors
MINTiP05030
STRINGi4932.YGL008C

Structurei

3D structure databases

ProteinModelPortaliP05030
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi5 – 17Poly-SerAdd BLAST13
Compositional biasi31 – 78Asp/Glu-rich (acidic)Add BLAST48
Compositional biasi39 – 44Poly-Asp6
Compositional biasi585 – 590Poly-Gly6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00910000144699
HOGENOMiHOG000160005
InParanoidiP05030
KOiK01535
OMAiHKYNVVE
OrthoDBiEOG092C0HLD

Family and domain databases

CDDicd02076 P-type_ATPase_H, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006534 P-type_ATPase_IIIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 2 hits
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 3 hits
TIGRFAMsiTIGR01647 ATPase-IIIA_H, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

P05030-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTSSSSSS SSASSVSAHQ PTQEKPAKTY DDAASESSDD DDIDALIEEL
60 70 80 90 100
QSNHGVDDED SDNDGPVAAG EARPVPEEYL QTDPSYGLTS DEVLKRRKKY
110 120 130 140 150
GLNQMADEKE SLVVKFVMFF VGPIQFVMEA AAILAAGLSD WVDFGVICGL
160 170 180 190 200
LMLNAGVGFV QEFQAGSIVD ELKKTLANTA VVIRDGQLVE IPANEVVPGD
210 220 230 240 250
ILQLEDGTVI PTDGRIVTED CFLQIDQSAI TGESLAVDKH YGDQTFSSST
260 270 280 290 300
VKRGEGFMVV TATGDNTFVG RAAALVNKAA GGQGHFTEVL NGIGIILLVL
310 320 330 340 350
VIATLLLVWT ACFYRTNGIV RILRYTLGIT IIGVPVGLPA VVTTTMAVGA
360 370 380 390 400
AYLAKKQAIV QKLSAIESLA GVEILCSDKT GTLTKNKLSL HEPYTVEGVS
410 420 430 440 450
PDDLMLTACL AASRKKKGLD AIDKAFLKSL KQYPKAKDAL TKYKVLEFHP
460 470 480 490 500
FDPVSKKVTA VVESPEGERI VCVKGAPLFV LKTVEEDHPI PEDVHENYEN
510 520 530 540 550
KVAELASRGF RALGVARKRG EGHWEILGVM PCMDPPRDDT AQTVSEARHL
560 570 580 590 600
GLRVKMLTGD AVGIAKETCR QLGLGTNIYN AERLGLGGGG DMPGSELADF
610 620 630 640 650
VENADGFAEV FPQHKYRVVE ILQNRGYLVA MTGDGVNDAP SLKKADTGIA
660 670 680 690 700
VEGATDAARS AADIVFLAPG LSAIIDALKT SRQIFHRMYS YVVYRIALSL
710 720 730 740 750
HLEIFLGLWI AILDNSLDID LIVFIAIFAD VATLAIAYDN APYSPKPVKW
760 770 780 790 800
NLPRLWGMSI ILGIVLAIGS WITLTTMFLP KGGIIQNFGA MNGIMFLQIS
810 820 830 840 850
LTENWLIFIT RAAGPFWSSI PSWQLAGAVF AVDIIATMFT LFGWWSENWT
860 870 880 890 900
DIVTVVRVWI WSIGIFCVLG GFYYEMSTSE AFDRLMNGKP MKEKKSTRSV
910
EDFMAAMQRV STQHEKET
Length:918
Mass (Da):99,619
Last modified:November 1, 1995 - v2
Checksum:iAA1E93966B37E8E1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03534 Genomic DNA Translation: CAA27237.1
Z72530 Genomic DNA Translation: CAA96708.1
BK006941 Genomic DNA Translation: DAA08090.1
PIRiS64010 PXBY1P
RefSeqiNP_011507.1, NM_001180873.1

Genome annotation databases

EnsemblFungiiYGL008C; YGL008C; YGL008C
GeneIDi852876
KEGGisce:YGL008C

Similar proteinsi

Entry informationi

Entry nameiPMA1_YEAST
AccessioniPrimary (citable) accession number: P05030
Secondary accession number(s): D6VUC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 198 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

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