P05029 (AT1B1_TORCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1

Gene names

Name:

atp1b1

Organism

Torpedo californica (Pacific electric ray)

Taxonomic identifier

7787 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Chondrichthyes › Elasmobranchii › Squalea › Hypnosqualea › Pristiorajea › Batoidea › Torpediniformes › Torpedinoidei › Torpedinidae › Torpedo

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Sodium/potassium-transporting ATPase subunit beta-1		PRO_0000219116
Regions
	Topological domain	1 – 32	32	Cytoplasmic Potential
	Transmembrane	33 – 53	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	54 – 305	252	Extracellular Potential
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) By similarity
	Glycosylation	194	1	N-linked (GlcNAc...) By similarity
	Glycosylation	267	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	127 ↔ 150		By similarity
	Disulfide bond	160 ↔ 176		By similarity
	Disulfide bond	215 ↔ 278

Sequences

	Sequence		Length	Mass (Da)
	P05029 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: BC13191F8D597563	FASTA	305	34,673
	10 20 30 40 50 60 MAREKSTDDG GGWKKFLWDS EKKQVLGRTG TSWFKIFVFY LIFYGCLAGI FIGTIQVMLL 70 80 90 100 110 120 TISDFEPKYQ DRVAPPGLSH SPYAVKTEIS FSVSNPNSYE NHVNGLKELL KNYNESKQDG 130 140 150 160 170 180 NTPFEDCGVI PADYITRGPI EESQGQKRVC RFLLQWLKNC SGIDDPSYGY SEGKPCIIAK 190 200 210 220 230 240 LNRILGFYPK PPKNGTDLPE ALQANYNQYV LPIHCQAKKE EDKVRIGTIE YFGMGGVGGF 250 260 270 280 290 300 PLQYYPYYGK RLQKNYLQPL VGIQFTNLTH NVELRVECKV FGDNIAYSEK DRSLGRFEVK IEVKS

References

[1]	"Primary structure of the beta-subunit of Torpedo californica (Na+ + K+)-ATPase deduced from the cDNA sequence." Noguchi S., Noda M., Takahashi H., Kawakami K., Ohta T., Nagano K., Hirose T., Inayama S., Kawamura M., Numa S. FEBS Lett. 196:315-320(1986) [PubMed: 3005037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of a disulfide between cysteine 214 and cysteine 277 in the beta subunit of native (Na+ + K+)ATPase." Kellaris K.V. Biochem. Biophys. Res. Commun. 162:64-70(1989) [PubMed: 2546555] [Abstract] Cited for: DISULFIDE BOND 215-CYS--CYS-278.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03471 mRNA. Translation: CAA27188.1.
PIR	PWRYNB. A23625. A33291.
3D structure databases
ProteinModelPortal	P05029.
SMR	P05029. Positions 29-74.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050603.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. IPR015565. ATPase_P-typ_Na/K-dep_b1su. [Graphical view]
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit. PTHR11523:SF10. Na/K_ATPaseBeta1. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AT1B1_TORCA

Accession

Primary (citable) accession number: P05029

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	August 10, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program