P05027 (AT1B1_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1

Gene names

Name:

ATP1B1

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4 By similarity.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 303

303

Sodium/potassium-transporting ATPase subunit beta-1

PRO_0000219099

Regions

Topological domain

1 – 34

34

Cytoplasmic Potential

Transmembrane

35 – 62

28

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

63 – 303

241

Extracellular Potential

Amino acid modifications

Modified residue

101

1

Phosphotyrosine By similarity

Glycosylation

158

1

N-linked (GlcNAc...) By similarity

Glycosylation

193

1

N-linked (GlcNAc...) By similarity

Glycosylation

265

1

N-linked (GlcNAc...) By similarity

Disulfide bond

126 ↔ 149

By similarity

Disulfide bond

159 ↔ 175

By similarity

Disulfide bond

213 ↔ 276

By similarity

Experimental info

Sequence conflict

15 – 16

2

FI → LM in AAA31001. Ref.3

Sequence conflict

151

1

F → S in CAA27575. Ref.1

Sequence conflict

159

1

C → S in AAA31001. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

303

Helix Strand Turn

Details...

Helix

34 – 37

4

Turn

38 – 42

5

Helix

55 – 58

4

Turn

59 – 61

3

Sequences

	Sequence		Length	Mass (Da)
	P05027 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 4B9650EDF5942350	FASTA	303	35,158
	10 20 30 40 50 60 MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF IGTIQVMLLT 70 80 90 100 110 120 ISEFKPTYQD RVAPPGLTQI PQSQKTEISF RPNDPQSYES YVVSIVRFLE KYKDLAQKDD 130 140 150 160 170 180 MIFEDCGNVP SELKERGEYN NERGERKVCR FRLEWLGNCS GLNDETYGYK DGKPCVIIKL 190 200 210 220 230 240 NRVLGFKPKP PKNESLETYP VMKYNPYVLP VHCTGKRDED KEKVGTMEYF GLGGYPGFPL 250 260 270 280 290 300 QYYPYYGKLL QPKYLQPLMA VQFTNLTMDT EIRIECKAYG ENIGYSEKDR FQGRFDVKIE VKS

References

[1]	"Pig kidney Na+,K+-ATPase. Primary structure and spatial organization." Ovchinnikov Y.A., Modyanov N.N., Broude N.E., Petrukhin K.E., Grishin A.V., Arzamazova N.M., Aldanova N.A., Monastyrskaya G.S., Sverdlov E.D. FEBS Lett. 201:237-245(1986) [PubMed: 2423371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Nucleotide sequence of cDNA and primary structure of the beta-subunit of Na+,K+-ATPase from pig kidneys." Ovchinnikov Y.A., Broude N.E., Petrukhin K.E., Grishin A.V., Kiyatkin N.I., Arzamazova N.M., Gevondyan N.M., Chertova E.N., Melkov A.M., Smirnov Y.V., Malyshev I.V., Monastyrskaya G.S., Modyanov N.N. Dokl. Biochem. 287:149-152(1986) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[3]	"Primary structure of the beta-subunit of Na+,K+-ATPase from the swine kidney. II. Reverse transcription, cloning of mRNA, complete nucleotide sequence corresponding to the structural region of the gene." Broude N.E., Monastyrskaya G.S., Petrukhin K.E., Grishin A.V., Kiyatkin N.I., Melkov A.M., Smirnov Y.V., Sverdiov V.E., Malyshev I.V., Modyanov N.N. Bioorg. Khim. 13:14-19(1987) [PubMed: 2436627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Detailed structural analysis of exposed domains of membrane-bound Na+,K+-ATPase. A model of transmembrane arrangement." Ovchinnikov Y.A., Arzamazova N.M., Arystarkhova E.A., Gevondyan N.M., Aldanova N.A., Modyanov N.N. FEBS Lett. 217:269-274(1987) [PubMed: 3036581] [Abstract] Cited for: TOPOLOGY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X03937 mRNA. Translation: CAA27575.1.
X04635 mRNA. Translation: CAA28301.1.
M38313 mRNA. Translation: AAA31001.1.

PIR

A24862.
I46571.
I47125.

RefSeq

NP_001001542.1. NM_001001542.1.

UniGene

Ssc.246.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3B8E	X-ray	3.50	B/D	28-73	[»]
3KDP	X-ray	3.50	B/D	18-303	[»]
3N23	X-ray	4.60	B/D	27-303	[»]
3N2F	X-ray	4.10	B/D	27-303	[»]

ProteinModelPortal

P05027.

SMR

P05027. Positions 28-73.

ModBase

Search...

Protein-protein interaction databases

STRING

P05027.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSSSCT00000006901; ENSSSCP00000006713; ENSSSCG00000006296.

GeneID

396898.

KEGG

ssc:396898.

Organism-specific databases

CTD

481.

Phylogenomic databases

HOVERGEN

HBG050603.

Family and domain databases

InterPro

IPR000402. ATPase_P-typ_cation-exchng_bsu.
IPR015565. ATPase_P-typ_Na/K-dep_b1su.
[Graphical view]

KO

K01540.

PANTHER

PTHR11523. ATPase_H_Na/K_b. 1 hit.
PTHR11523:SF10. Na/K_ATPaseBeta1. 1 hit.

Pfam

PF00287. Na_K-ATPase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01107. Na_K_ATPase_bet. 1 hit.

PROSITE

PS00390. ATPASE_NA_K_BETA_1. 1 hit.
PS00391. ATPASE_NA_K_BETA_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

AT1B1_PIG

Accession

Primary (citable) accession number: P05027

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program