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Protein

Sodium/potassium-transporting ATPase subunit beta-1

Gene

ATP1B1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Involved in cell adhesion and establishing epithelial cell polarity.By similarity

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL
  • sodium:potassium-exchanging ATPase activity Source: BHF-UCL

GO - Biological processi

  • ATP hydrolysis coupled transmembrane transport Source: BHF-UCL
  • cell adhesion Source: UniProtKB-KW
  • cellular potassium ion homeostasis Source: BHF-UCL
  • cellular sodium ion homeostasis Source: BHF-UCL
  • establishment or maintenance of transmembrane electrochemical gradient Source: BHF-UCL
  • membrane repolarization Source: BHF-UCL
  • potassium ion import Source: BHF-UCL
  • potassium ion import across plasma membrane Source: BHF-UCL
  • sodium ion export from cell Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1
Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1
Gene namesi
Name:ATP1B1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34CytoplasmicSequence analysisAdd BLAST34
Transmembranei35 – 62Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST28
Topological domaini63 – 303ExtracellularSequence analysisAdd BLAST241

GO - Cellular componenti

  • sodium:potassium-exchanging ATPase complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002190991 – 303Sodium/potassium-transporting ATPase subunit beta-1Add BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineBy similarity1
Modified residuei101PhosphotyrosineBy similarity1
Disulfide bondi126 ↔ 149By similarity
Glycosylationi158N-linked (GlcNAc...)By similarity1
Disulfide bondi159 ↔ 175By similarity
Glycosylationi193N-linked (GlcNAc...)By similarity1
Disulfide bondi213 ↔ 276By similarity
Glycosylationi265N-linked (GlcNAc...)By similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP05027.
PeptideAtlasiP05027.
PRIDEiP05027.

Expressioni

Gene expression databases

BgeeiENSSSCG00000006296.
GenevisibleiP05027. SS.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4 (By similarity). Interacts with MLC1. Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM. Interacts with KIRREL3 (By similarity).By similarity

GO - Molecular functioni

  • ATPase binding Source: BHF-UCL

Protein-protein interaction databases

DIPiDIP-60366N.
IntActiP05027. 1 interactor.
STRINGi9823.ENSSSCP00000006713.

Structurei

Secondary structure

1303
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 15Combined sources4
Beta strandi19 – 21Combined sources3
Beta strandi24 – 27Combined sources4
Helixi31 – 58Combined sources28
Turni59 – 61Combined sources3
Beta strandi63 – 65Combined sources3
Helixi70 – 72Combined sources3
Beta strandi77 – 81Combined sources5
Beta strandi84 – 89Combined sources6
Helixi96 – 98Combined sources3
Helixi99 – 109Combined sources11
Turni114 – 116Combined sources3
Turni120 – 122Combined sources3
Beta strandi139 – 143Combined sources5
Turni153 – 155Combined sources3
Beta strandi156 – 160Combined sources5
Beta strandi164 – 166Combined sources3
Beta strandi171 – 173Combined sources3
Beta strandi175 – 180Combined sources6
Beta strandi193 – 195Combined sources3
Beta strandi200 – 202Combined sources3
Turni205 – 207Combined sources3
Beta strandi208 – 213Combined sources6
Beta strandi215 – 220Combined sources6
Beta strandi227 – 230Combined sources4
Helixi232 – 234Combined sources3
Beta strandi236 – 239Combined sources4
Helixi240 – 242Combined sources3
Helixi247 – 250Combined sources4
Beta strandi258 – 262Combined sources5
Beta strandi269 – 274Combined sources6
Beta strandi276 – 278Combined sources3
Beta strandi280 – 282Combined sources3
Beta strandi292 – 294Combined sources3
Beta strandi297 – 300Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B8EX-ray3.50B/D28-73[»]
3KDPX-ray3.50B/D18-303[»]
3N23X-ray4.60B/D27-303[»]
3WGUX-ray2.80B/D1-303[»]
3WGVX-ray2.80B/D1-303[»]
4HQJX-ray4.30B/D1-303[»]
4HYTX-ray3.40B/D1-303[»]
4RESX-ray3.41B/D1-303[»]
4RETX-ray4.00B/D1-303[»]
ProteinModelPortaliP05027.
SMRiP05027.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP05027.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 303immunoglobulin-likeBy similarityAdd BLAST113

Domaini

The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3927. Eukaryota.
ENOG411150A. LUCA.
HOGENOMiHOG000039248.
HOVERGENiHBG050603.
InParanoidiP05027.
KOiK01540.
OrthoDBiEOG091G0DJ4.

Family and domain databases

InterProiIPR000402. Na/K_ATPase_sub_beta.
IPR015565. Na/K_ATPase_sub_beta_chordates.
[Graphical view]
PANTHERiPTHR11523. PTHR11523. 1 hit.
PTHR11523:SF10. PTHR11523:SF10. 1 hit.
PfamiPF00287. Na_K-ATPase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITEiPS00390. ATPASE_NA_K_BETA_1. 1 hit.
PS00391. ATPASE_NA_K_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P05027-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF
60 70 80 90 100
IGTIQVMLLT ISEFKPTYQD RVAPPGLTQI PQSQKTEISF RPNDPQSYES
110 120 130 140 150
YVVSIVRFLE KYKDLAQKDD MIFEDCGNVP SELKERGEYN NERGERKVCR
160 170 180 190 200
FRLEWLGNCS GLNDETYGYK DGKPCVIIKL NRVLGFKPKP PKNESLETYP
210 220 230 240 250
VMKYNPYVLP VHCTGKRDED KEKVGTMEYF GLGGYPGFPL QYYPYYGKLL
260 270 280 290 300
QPKYLQPLMA VQFTNLTMDT EIRIECKAYG ENIGYSEKDR FQGRFDVKIE

VKS
Length:303
Mass (Da):35,158
Last modified:October 1, 1996 - v2
Checksum:i4B9650EDF5942350
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15 – 16FI → LM in AAA31001 (PubMed:2436627).Curated2
Sequence conflicti151F → S in CAA27575 (PubMed:2423371).Curated1
Sequence conflicti159C → S in AAA31001 (PubMed:2436627).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03937 mRNA. Translation: CAA27575.1.
X04635 mRNA. Translation: CAA28301.1.
M38313 mRNA. Translation: AAA31001.1.
PIRiA24862.
I46571.
I47125.
RefSeqiNP_001001542.1. NM_001001542.1.
XP_013852243.1. XM_013996789.1.
UniGeneiSsc.246.

Genome annotation databases

GeneIDi106510095.
396898.
KEGGissc:106510095.
ssc:396898.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03937 mRNA. Translation: CAA27575.1.
X04635 mRNA. Translation: CAA28301.1.
M38313 mRNA. Translation: AAA31001.1.
PIRiA24862.
I46571.
I47125.
RefSeqiNP_001001542.1. NM_001001542.1.
XP_013852243.1. XM_013996789.1.
UniGeneiSsc.246.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B8EX-ray3.50B/D28-73[»]
3KDPX-ray3.50B/D18-303[»]
3N23X-ray4.60B/D27-303[»]
3WGUX-ray2.80B/D1-303[»]
3WGVX-ray2.80B/D1-303[»]
4HQJX-ray4.30B/D1-303[»]
4HYTX-ray3.40B/D1-303[»]
4RESX-ray3.41B/D1-303[»]
4RETX-ray4.00B/D1-303[»]
ProteinModelPortaliP05027.
SMRiP05027.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60366N.
IntActiP05027. 1 interactor.
STRINGi9823.ENSSSCP00000006713.

Proteomic databases

PaxDbiP05027.
PeptideAtlasiP05027.
PRIDEiP05027.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi106510095.
396898.
KEGGissc:106510095.
ssc:396898.

Organism-specific databases

CTDi481.

Phylogenomic databases

eggNOGiKOG3927. Eukaryota.
ENOG411150A. LUCA.
HOGENOMiHOG000039248.
HOVERGENiHBG050603.
InParanoidiP05027.
KOiK01540.
OrthoDBiEOG091G0DJ4.

Miscellaneous databases

EvolutionaryTraceiP05027.

Gene expression databases

BgeeiENSSSCG00000006296.
GenevisibleiP05027. SS.

Family and domain databases

InterProiIPR000402. Na/K_ATPase_sub_beta.
IPR015565. Na/K_ATPase_sub_beta_chordates.
[Graphical view]
PANTHERiPTHR11523. PTHR11523. 1 hit.
PTHR11523:SF10. PTHR11523:SF10. 1 hit.
PfamiPF00287. Na_K-ATPase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITEiPS00390. ATPASE_NA_K_BETA_1. 1 hit.
PS00391. ATPASE_NA_K_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT1B1_PIG
AccessioniPrimary (citable) accession number: P05027
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.