P05026 (AT1B1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-1

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-1

Gene names

Name:	ATP1B1
Synonyms:	ATP1B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Ref.9 Involved in cell adhesion and establishing epithelial cell polarity. Ref.9
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4 By similarity. Interacts with MLC1. Part of a complex containing MLC1, TRPV4, AQP4 and HEPACAM. Ref.13
Subcellular location	Cell membrane; Single-pass type II membrane protein.
Tissue specificity	Found in most tissues.
Domain	The C-terminal lobe folds into an immunoglobulin-like domain and mediates cell adhesion properties By similarity. Ref.9
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Cell adhesion Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome leukocyte migration Traceable author statement. Source: Reactome response to hypoxia Inferred from electronic annotation. Source: Compara
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from electronic annotation. Source: Compara caveola Inferred from electronic annotation. Source: Compara sodium:potassium-exchanging ATPase complex Traceable author statement Ref.2. Source: ProtInc
Molecular_function	sodium:potassium-exchanging ATPase activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P05026-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P05026-2)

The sequence of this isoform differs from the canonical sequence as follows:
     300-303: EVKS → KF

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Sodium/potassium-transporting ATPase subunit beta-1		PRO_0000219097
Regions
	Topological domain	1 – 34	34	Cytoplasmic Potential
	Transmembrane	35 – 62	28	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	63 – 303	241	Extracellular Potential
	Region	191 – 303	113	immunoglobulin-like
Amino acid modifications
	Modified residue	101	1	Phosphotyrosine By similarity
	Glycosylation	158	1	N-linked (GlcNAc...) Ref.8 Ref.10
	Glycosylation	193	1	N-linked (GlcNAc...) Ref.8 Ref.10
	Glycosylation	265	1	N-linked (GlcNAc...) Ref.8 Ref.10 Ref.11
	Disulfide bond	126 ↔ 149		By similarity
	Disulfide bond	159 ↔ 175		By similarity
	Disulfide bond	213 ↔ 276		By similarity
Natural variations
	Alternative sequence	300 – 303	4	EVKS → KF in isoform 2.		VSP_000349

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 107D3C04394F2D11	FASTA	303	35,061
	10 20 30 40 50 60 MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF IGTIQVMLLT 70 80 90 100 110 120 ISEFKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA YVLNIVRFLE KYKDSAQRDD 130 140 150 160 170 180 MIFEDCGDVP SEPKERGDFN HERGERKVCR FKLEWLGNCS GLNDETYGYK EGKPCIIIKL 190 200 210 220 230 240 NRVLGFKPKP PKNESLETYP VMKYNPNVLP VQCTGKRDED KDKVGNVEYF GLGNSPGFPL 250 260 270 280 290 300 QYYPYYGKLL QPKYLQPLLA VQFTNLTMDT EIRIECKAYG ENIGYSEKDR FQGRFDVKIE VKS
	Isoform 2 [UniParc]. Checksum: 670C194F2D114F67	FASTA	301	34,893
	10 20 30 40 50 60 MARGKAKEEG SWKKFIWNSE KKEFLGRTGG SWFKILLFYV IFYGCLAGIF IGTIQVMLLT 70 80 90 100 110 120 ISEFKPTYQD RVAPPGLTQI PQIQKTEISF RPNDPKSYEA YVLNIVRFLE KYKDSAQRDD 130 140 150 160 170 180 MIFEDCGDVP SEPKERGDFN HERGERKVCR FKLEWLGNCS GLNDETYGYK EGKPCIIIKL 190 200 210 220 230 240 NRVLGFKPKP PKNESLETYP VMKYNPNVLP VQCTGKRDED KDKVGNVEYF GLGNSPGFPL 250 260 270 280 290 300 QYYPYYGKLL QPKYLQPLLA VQFTNLTMDT EIRIECKAYG ENIGYSEKDR FQGRFDVKIK F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of human Na,K-ATPase beta-subunit." Kawakami K., Nojima H., Ohta T., Nagano K. Nucleic Acids Res. 14:2833-2844(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Characterization of two genes for the human Na,K-ATPase beta subunit." Lane L.K., Shull M.M., Whitmer K.R., Lingrel J.B. Genomics 5:445-453(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization and quantification of full-length and truncated Na,K-ATPase alpha 1 and beta 1 RNA transcripts expressed in human retinal pigment epithelium." Ruiz A., Bhat S.P., Bok D. Gene 155:179-184(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Retinal pigment epithelium.
[4]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Kidney.
[7]	"Human Na(+), K(+)-ATPase genes. Beta subunit gene family contains at least one gene and one pseudogene." Ushkaryov Y.A., Monastyrskaya G.S., Broude N.E., Nikiforova N.N., Bessarab B.A., Orlova M.Y., Petrukhin K.E., Modyanov N.N., Sverdlov E.D. FEBS Lett. 257:439-442(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 34-72. Tissue: Sperm.
[8]	"Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry." Zhang H., Li X.-J., Martin D.B., Aebersold R. Nat. Biotechnol. 21:660-666(2003) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-158; ASN-193 AND ASN-265.
[9]	"A C-terminal lobe of the beta subunit of Na,K-ATPase and H,K-ATPase resembles cell adhesion molecules." Bab-Dinitz E., Albeck S., Peleg Y., Brumfeld V., Gottschalk K.E., Karlish S.J. Biochemistry 48:8684-8691(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN ADHESION, IMMUNOGLOBULIN-LIKE DOMAIN.
[10]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-158; ASN-193 AND ASN-265, MASS SPECTROMETRY. Tissue: Liver.
[11]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-265, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Megalencephalic leukoencephalopathy with subcortical cysts protein 1 functionally cooperates with the TRPV4 cation channel to activate the response of astrocytes to osmotic stress: dysregulation by pathological mutations." Lanciotti A., Brignone M.S., Molinari P., Visentin S., De Nuccio C., Macchia G., Aiello C., Bertini E., Aloisi F., Petrucci T.C., Ambrosini E. Hum. Mol. Genet. 21:2166-2180(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MLC1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03747 mRNA. Translation: CAA27385.1. M25160, M25161 Genomic DNA. Translation: AAA36352.1. U16799 mRNA. Translation: AAC50132.1. BT009787 mRNA. Translation: AAP88789.1. AL031726 Genomic DNA. Translation: CAI18888.1. BC000006 mRNA. Translation: AAH00006.1. X17161 Genomic DNA. Translation: CAA35040.1.
IPI	IPI00747849. IPI00871221.
PIR	PWHUNB. A23764.
RefSeq	NP_001668.1. NM_001677.3.
UniGene	Hs.291196.
3D structure databases
ProteinModelPortal	P05026.
ModBase	Search...
Protein-protein interaction databases
IntAct	P05026. 4 interactions.
MINT	MINT-5000887.
STRING	9606.ENSP00000356789.
Protein family/group databases
TCDB	3.A.3.1.1. P-type ATPase (P-ATPase) superfamily.
PTM databases
PhosphoSite	P05026.
Polymorphism databases
DMDM	114392.
Proteomic databases
PaxDb	P05026.
PRIDE	P05026.
Protocols and materials databases
DNASU	481.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367815; ENSP00000356789; ENSG00000143153. ENST00000367816; ENSP00000356790; ENSG00000143153.
GeneID	481.
KEGG	hsa:481.
UCSC	uc001gfr.1. human.
Organism-specific databases
CTD	481.
GeneCards	GC01P169074.
H-InvDB	HIX0001311.
HGNC	HGNC:804. ATP1B1.
HPA	HPA012911.
MIM	182330. gene.
neXtProt	NX_P05026.
PharmGKB	PA66.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG313139.
HOGENOM	HOG000039248.
HOVERGEN	HBG050603.
InParanoid	P05026.
KO	K01540.
OMA	GFKPKPP.
OrthoDB	EOG4M3998.
PhylomeDB	P05026.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P05026.
Bgee	P05026.
CleanEx	HS_ATP1B1.
Genevestigator	P05026.
GermOnline	ENSG00000143153. Homo sapiens.
Family and domain databases
InterPro	IPR000402. Na/K_ATPase_sub_beta. IPR015565. Na/K_ATPase_sub_beta_chordates. [Graphical view]
PANTHER	PTHR11523. PTHR11523. 1 hit. PTHR11523:SF10. PTHR11523:SF10. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P05026.
ChEMBL	CHEMBL2379.
ChiTaRS	ATP1B1. human.
GenomeRNAi	481.
NextBio	1995.
SOURCE	Search...

Entry information

Entry name

AT1B1_HUMAN

Accession

Primary (citable) accession number: P05026
Secondary accession number(s): Q5TGZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 140 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.