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P05025

- AT1A_TORCA

UniProt

P05025 - AT1A_TORCA

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Protein

Sodium/potassium-transporting ATPase subunit alpha

Gene
N/A
Organism
Torpedo californica (Pacific electric ray)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei376 – 37614-aspartylphosphate intermediateBy similarity
Metal bindingi716 – 7161MagnesiumBy similarity
Metal bindingi720 – 7201MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. sodium:potassium-exchanging ATPase activity Source: UniProtKB-EC

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha subunit
Alternative name(s):
Sodium pump subunit alpha
OrganismiTorpedo californica (Pacific electric ray)
Taxonomic identifieri7787 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiBatoideaTorpediniformesTorpedinidaeTorpedo

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 55PRO_0000002509
Chaini6 – 10221017Sodium/potassium-transporting ATPase subunit alphaPRO_0000002510Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161Phosphoserine; by PKCBy similarity
Modified residuei942 – 9421Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP05025.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma.

Structurei

3D structure databases

ProteinModelPortaliP05025.
SMRiP05025. Positions 28-1022.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini6 – 8782CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini109 – 13123LumenalSequence AnalysisAdd
BLAST
Topological domaini153 – 288136CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini309 – 32012LumenalSequence AnalysisAdd
BLAST
Topological domaini339 – 771433CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini792 – 80110LumenalSequence Analysis
Topological domaini823 – 84220CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini866 – 91752LumenalSequence AnalysisAdd
BLAST
Topological domaini938 – 95013CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini970 – 98415LumenalSequence AnalysisAdd
BLAST
Topological domaini1006 – 102217CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Transmembranei132 – 15221HelicalSequence AnalysisAdd
BLAST
Transmembranei289 – 30820HelicalSequence AnalysisAdd
BLAST
Transmembranei321 – 33818HelicalSequence AnalysisAdd
BLAST
Transmembranei772 – 79120HelicalSequence AnalysisAdd
BLAST
Transmembranei802 – 82221HelicalSequence AnalysisAdd
BLAST
Transmembranei843 – 86523HelicalSequence AnalysisAdd
BLAST
Transmembranei918 – 93720HelicalSequence AnalysisAdd
BLAST
Transmembranei951 – 96919HelicalSequence AnalysisAdd
BLAST
Transmembranei985 – 100521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 843Interaction with phosphoinositide-3 kinaseBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG004298.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P05025-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKGAASEKY QPAATSENAK NSKKSKSKTT DLDELKKEVS LDDHKLNLDE
60 70 80 90 100
LHQKYGTDLT QGLTPARAKE ILARDGPNAL TPPPTTPEWI KFCRQLFGGF
110 120 130 140 150
SILLWTGAIL CFLAYGIQVA TVDNPANDNL YLGVVLSTVV IITGCFSYYQ
160 170 180 190 200
EAKSSKIMDS FKNMVPQQAL VIRDGEKSSI NAEQVVVGDL VEVKGGDRIP
210 220 230 240 250
ADLRIISACS CKVDNSSLTG ESEPQSRSPE YSSENPLETK NIAFFSTNCV
260 270 280 290 300
EGTARGIVIN IGDHTVMGRI ATLASGLEVG QTPIAAEIEH FIHIITGVAV
310 320 330 340 350
FLGVSFFILS LILGYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR
360 370 380 390 400
MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA
410 420 430 440 450
DTTENQSGIS FDKTSLSWNA LSRIAALCNR AVFQAGQDSV PILKRSVAGD
460 470 480 490 500
ASESALLKCI ELCCGSVSQM RDRNPKIVEI PFNSTNKYQL SIHENDKADS
510 520 530 540 550
RYLLVMKGAP ERILDRCSTI LLNGEDKPLN EEMKEAFQNA YLELGGLGER
560 570 580 590 600
VLGFCHLKLS TSKFPEGYPF DVEEPNFPIT DLCFVGLMSM IDPPRAAVPD
610 620 630 640 650
AVGKCRSAGI KVIMVTGDHP ITAKAIAKGV GIISEGNETV EDIAARLNIP
660 670 680 690 700
VNQVNPRDAK ACVVHGTDLK DLSHENLDDI LHYHTEIVFA RTSPQQKLII
710 720 730 740 750
VEGCQRQGAI VAVTGDGVND SPALKKADIG VAMGIAGSDV SKQAADMILL
760 770 780 790 800
DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEITPFL VFIIANVPLP
810 820 830 840 850
LGTVTILCID LGTDMVPAIS LAYERAESDI MKRQPRNPKT DKLVNERLIS
860 870 880 890 900
MAYGQIGMIQ ALGGFFSYFV ILAENGFLPI DLIGIREKWD ELWTQDLEDS
910 920 930 940 950
YGQQWTYEQR KIVEYTCHTS FFVSIVIVQW ADLIICKTRR NSIFQQGMKN
960 970 980 990 1000
KILIFGLFEE TALAAFLSYT PGTDIALRMY PLKPSWWFCA FPYSLIIFLY
1010 1020
DEARRFILRR NPGGWVEQET YY
Length:1,022
Mass (Da):112,430
Last modified:August 13, 1987 - v1
Checksum:iD92FE737847D73C2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02810 mRNA. Translation: CAA26578.1.
PIRiS00503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02810 mRNA. Translation: CAA26578.1 .
PIRi S00503.

3D structure databases

ProteinModelPortali P05025.
SMRi P05025. Positions 28-1022.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P05025.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG004298.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the alpha-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequence."
    Kawakami K., Noguchi S., Noda M., Takahashi H., Ohta T., Kawamura M., Nojima H., Nagano K., Hirose T., Inayama S., Hayashida H., Miyata T., Numa S.
    Nature 316:733-736(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "The active site structure of Na+/K+-transporting ATPase: location of the 5'-(p-fluorosulfonyl)benzoyladenosine binding site and soluble peptides released by trypsin."
    Ohta T., Nagano K., Yoshida M.
    Proc. Natl. Acad. Sci. U.S.A. 83:2071-2075(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 386-402; 502-512; 671-689 AND 887-906.

Entry informationi

Entry nameiAT1A_TORCA
AccessioniPrimary (citable) accession number: P05025
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 1, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3