Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P05025

- AT1A_TORCA

UniProt

P05025 - AT1A_TORCA

Protein

Sodium/potassium-transporting ATPase subunit alpha

Gene
N/A
Organism
Torpedo californica (Pacific electric ray)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei376 – 37614-aspartylphosphate intermediateBy similarity
    Metal bindingi716 – 7161MagnesiumBy similarity
    Metal bindingi720 – 7201MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. sodium:potassium-exchanging ATPase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha subunit
    Alternative name(s):
    Sodium pump subunit alpha
    OrganismiTorpedo californica (Pacific electric ray)
    Taxonomic identifieri7787 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiBatoideaTorpediniformesTorpedinidaeTorpedo

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 55PRO_0000002509
    Chaini6 – 10221017Sodium/potassium-transporting ATPase subunit alphaPRO_0000002510Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei16 – 161Phosphoserine; by PKCBy similarity
    Modified residuei942 – 9421Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP05025.

    Interactioni

    Subunit structurei

    Composed of three subunits: alpha (catalytic), beta and gamma.

    Structurei

    3D structure databases

    ProteinModelPortaliP05025.
    SMRiP05025. Positions 28-1022.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini6 – 8782CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini109 – 13123LumenalSequence AnalysisAdd
    BLAST
    Topological domaini153 – 288136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini309 – 32012LumenalSequence AnalysisAdd
    BLAST
    Topological domaini339 – 771433CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini792 – 80110LumenalSequence Analysis
    Topological domaini823 – 84220CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini866 – 91752LumenalSequence AnalysisAdd
    BLAST
    Topological domaini938 – 95013CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini970 – 98415LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1006 – 102217CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei88 – 10821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei132 – 15221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei289 – 30820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei321 – 33818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei772 – 79120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei802 – 82221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei843 – 86523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei918 – 93720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei951 – 96919HelicalSequence AnalysisAdd
    BLAST
    Transmembranei985 – 100521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni82 – 843Interaction with phosphoinositide-3 kinaseBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG004298.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05025-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKGAASEKY QPAATSENAK NSKKSKSKTT DLDELKKEVS LDDHKLNLDE     50
    LHQKYGTDLT QGLTPARAKE ILARDGPNAL TPPPTTPEWI KFCRQLFGGF 100
    SILLWTGAIL CFLAYGIQVA TVDNPANDNL YLGVVLSTVV IITGCFSYYQ 150
    EAKSSKIMDS FKNMVPQQAL VIRDGEKSSI NAEQVVVGDL VEVKGGDRIP 200
    ADLRIISACS CKVDNSSLTG ESEPQSRSPE YSSENPLETK NIAFFSTNCV 250
    EGTARGIVIN IGDHTVMGRI ATLASGLEVG QTPIAAEIEH FIHIITGVAV 300
    FLGVSFFILS LILGYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR 350
    MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA 400
    DTTENQSGIS FDKTSLSWNA LSRIAALCNR AVFQAGQDSV PILKRSVAGD 450
    ASESALLKCI ELCCGSVSQM RDRNPKIVEI PFNSTNKYQL SIHENDKADS 500
    RYLLVMKGAP ERILDRCSTI LLNGEDKPLN EEMKEAFQNA YLELGGLGER 550
    VLGFCHLKLS TSKFPEGYPF DVEEPNFPIT DLCFVGLMSM IDPPRAAVPD 600
    AVGKCRSAGI KVIMVTGDHP ITAKAIAKGV GIISEGNETV EDIAARLNIP 650
    VNQVNPRDAK ACVVHGTDLK DLSHENLDDI LHYHTEIVFA RTSPQQKLII 700
    VEGCQRQGAI VAVTGDGVND SPALKKADIG VAMGIAGSDV SKQAADMILL 750
    DDNFASIVTG VEEGRLIFDN LKKSIAYTLT SNIPEITPFL VFIIANVPLP 800
    LGTVTILCID LGTDMVPAIS LAYERAESDI MKRQPRNPKT DKLVNERLIS 850
    MAYGQIGMIQ ALGGFFSYFV ILAENGFLPI DLIGIREKWD ELWTQDLEDS 900
    YGQQWTYEQR KIVEYTCHTS FFVSIVIVQW ADLIICKTRR NSIFQQGMKN 950
    KILIFGLFEE TALAAFLSYT PGTDIALRMY PLKPSWWFCA FPYSLIIFLY 1000
    DEARRFILRR NPGGWVEQET YY 1022
    Length:1,022
    Mass (Da):112,430
    Last modified:August 13, 1987 - v1
    Checksum:iD92FE737847D73C2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02810 mRNA. Translation: CAA26578.1.
    PIRiS00503.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02810 mRNA. Translation: CAA26578.1 .
    PIRi S00503.

    3D structure databases

    ProteinModelPortali P05025.
    SMRi P05025. Positions 28-1022.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P05025.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG004298.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the alpha-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequence."
      Kawakami K., Noguchi S., Noda M., Takahashi H., Ohta T., Kawamura M., Nojima H., Nagano K., Hirose T., Inayama S., Hayashida H., Miyata T., Numa S.
      Nature 316:733-736(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "The active site structure of Na+/K+-transporting ATPase: location of the 5'-(p-fluorosulfonyl)benzoyladenosine binding site and soluble peptides released by trypsin."
      Ohta T., Nagano K., Yoshida M.
      Proc. Natl. Acad. Sci. U.S.A. 83:2071-2075(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 386-402; 502-512; 671-689 AND 887-906.

    Entry informationi

    Entry nameiAT1A_TORCA
    AccessioniPrimary (citable) accession number: P05025
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3