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P05020

- PYRC_ECOLI

UniProt

P05020 - PYRC_ECOLI

Protein

Dihydroorotase

Gene

pyrC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 142 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate.

    Cofactori

    Binds 2 zinc ions per subunit.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi17 – 171Zinc 1; via tele nitrogen
    Metal bindingi19 – 191Zinc 1; via tele nitrogen
    Metal bindingi103 – 1031Zinc 1; via carbamate group
    Metal bindingi103 – 1031Zinc 2; via carbamate group
    Metal bindingi140 – 1401Zinc 2; via pros nitrogen
    Metal bindingi178 – 1781Zinc 2; via tele nitrogen
    Metal bindingi251 – 2511Zinc 1

    GO - Molecular functioni

    1. dihydroorotase activity Source: EcoCyc
    2. zinc ion binding Source: EcoCyc

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: EcoCyc
    2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:DIHYDROOROT-MONOMER.
    ECOL316407:JW1049-MONOMER.
    MetaCyc:DIHYDROOROT-MONOMER.
    BRENDAi3.5.2.3. 2026.
    SABIO-RKP05020.
    UniPathwayiUPA00070; UER00117.

    Protein family/group databases

    MEROPSiM38.A02.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydroorotase (EC:3.5.2.3)
    Short name:
    DHOase
    Gene namesi
    Name:pyrC
    Ordered Locus Names:b1062, JW1049
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10806. pyrC.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 348347DihydroorotasePRO_0000147205Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei103 – 1031N6-carboxylysine1 Publication

    Proteomic databases

    PaxDbiP05020.
    PRIDEiP05020.

    Expressioni

    Gene expression databases

    GenevestigatoriP05020.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-10624N.
    IntActiP05020. 6 interactions.
    STRINGi511145.b1062.

    Structurei

    Secondary structure

    1
    348
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 115
    Beta strandi15 – 184
    Helixi23 – 3412
    Beta strandi38 – 425
    Helixi53 – 6513
    Beta strandi74 – 807
    Helixi87 – 959
    Beta strandi98 – 1047
    Turni113 – 1153
    Helixi120 – 1234
    Helixi124 – 13310
    Beta strandi137 – 1393
    Helixi150 – 1523
    Helixi153 – 1608
    Helixi162 – 1687
    Turni169 – 1713
    Beta strandi174 – 1763
    Helixi182 – 1898
    Beta strandi195 – 1995
    Helixi201 – 2055
    Helixi208 – 2125
    Helixi218 – 2203
    Helixi229 – 24012
    Beta strandi246 – 2483
    Helixi257 – 2604
    Beta strandi261 – 2655
    Turni272 – 2743
    Helixi275 – 28511
    Helixi289 – 2913
    Helixi292 – 2976
    Helixi299 – 3046
    Beta strandi312 – 3165
    Beta strandi329 – 3335
    Turni336 – 3394
    Beta strandi341 – 3433

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1J79X-ray1.70A/B2-348[»]
    1XGEX-ray1.90A/B2-348[»]
    2E25X-ray2.70A2-348[»]
    2EG6X-ray1.70A/B2-348[»]
    2EG7X-ray2.00A/B2-348[»]
    2EG8X-ray2.20A/B2-348[»]
    2Z24X-ray1.90A/B2-348[»]
    2Z25X-ray1.87A/B2-348[»]
    2Z26X-ray1.29A/B2-348[»]
    2Z27X-ray1.87A/B2-348[»]
    2Z28X-ray1.87A/B2-348[»]
    2Z29X-ray1.90A/B2-348[»]
    2Z2AX-ray1.87A/B2-348[»]
    2Z2BX-ray1.85A2-348[»]
    ProteinModelPortaliP05020.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP05020.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DHOase family. Type 1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0418.
    HOGENOMiHOG000256259.
    KOiK01465.
    OMAiLKRNVHQ.
    OrthoDBiEOG6TFCMH.
    PhylomeDBiP05020.

    Family and domain databases

    HAMAPiMF_00219. PyrC_type1.
    InterProiIPR006680. Amidohydro_1.
    IPR004721. DHOdimr.
    IPR002195. Dihydroorotase_CS.
    [Graphical view]
    PfamiPF01979. Amidohydro_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001237. DHOdimr. 1 hit.
    TIGRFAMsiTIGR00856. pyrC_dimer. 1 hit.
    PROSITEiPS00482. DIHYDROOROTASE_1. 1 hit.
    PS00483. DIHYDROOROTASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P05020-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAPSQVLKI RRPDDWHLHL RDGDMLKTVV PYTSEIYGRA IVMPNLAPPV    50
    TTVEAAVAYR QRILDAVPAG HDFTPLMTCY LTDSLDPNEL ERGFNEGVFT 100
    AAKLYPANAT TNSSHGVTSI DAIMPVLERM EKIGMPLLVH GEVTHADIDI 150
    FDREARFIES VMEPLRQRLT ALKVVFEHIT TKDAADYVRD GNERLAATIT 200
    PQHLMFNRNH MLVGGVRPHL YCLPILKRNI HQQALRELVA SGFNRVFLGT 250
    DSAPHARHRK ESSCGCAGCF NAPTALGSYA TVFEEMNALQ HFEAFCSVNG 300
    PQFYGLPVND TFIELVREEQ QVAESIALTD DTLVPFLAGE TVRWSVKQ 348
    Length:348
    Mass (Da):38,827
    Last modified:January 23, 2007 - v2
    Checksum:i179B35324204A111
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04469 Genomic DNA. Translation: CAA28157.1.
    M16752 Genomic DNA. Translation: AAA24482.1.
    U00096 Genomic DNA. Translation: AAC74146.1.
    AP009048 Genomic DNA. Translation: BAA35870.1.
    D31709 Genomic DNA. Translation: BAA06514.1.
    PIRiA25008. DEECOO.
    RefSeqiNP_415580.1. NC_000913.3.
    YP_489330.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74146; AAC74146; b1062.
    BAA35870; BAA35870; BAA35870.
    GeneIDi12932767.
    945787.
    KEGGiecj:Y75_p1032.
    eco:b1062.
    PATRICi32117363. VBIEscCol129921_1104.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04469 Genomic DNA. Translation: CAA28157.1 .
    M16752 Genomic DNA. Translation: AAA24482.1 .
    U00096 Genomic DNA. Translation: AAC74146.1 .
    AP009048 Genomic DNA. Translation: BAA35870.1 .
    D31709 Genomic DNA. Translation: BAA06514.1 .
    PIRi A25008. DEECOO.
    RefSeqi NP_415580.1. NC_000913.3.
    YP_489330.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1J79 X-ray 1.70 A/B 2-348 [» ]
    1XGE X-ray 1.90 A/B 2-348 [» ]
    2E25 X-ray 2.70 A 2-348 [» ]
    2EG6 X-ray 1.70 A/B 2-348 [» ]
    2EG7 X-ray 2.00 A/B 2-348 [» ]
    2EG8 X-ray 2.20 A/B 2-348 [» ]
    2Z24 X-ray 1.90 A/B 2-348 [» ]
    2Z25 X-ray 1.87 A/B 2-348 [» ]
    2Z26 X-ray 1.29 A/B 2-348 [» ]
    2Z27 X-ray 1.87 A/B 2-348 [» ]
    2Z28 X-ray 1.87 A/B 2-348 [» ]
    2Z29 X-ray 1.90 A/B 2-348 [» ]
    2Z2A X-ray 1.87 A/B 2-348 [» ]
    2Z2B X-ray 1.85 A 2-348 [» ]
    ProteinModelPortali P05020.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10624N.
    IntActi P05020. 6 interactions.
    STRINGi 511145.b1062.

    Chemistry

    BindingDBi P05020.

    Protein family/group databases

    MEROPSi M38.A02.

    Proteomic databases

    PaxDbi P05020.
    PRIDEi P05020.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74146 ; AAC74146 ; b1062 .
    BAA35870 ; BAA35870 ; BAA35870 .
    GeneIDi 12932767.
    945787.
    KEGGi ecj:Y75_p1032.
    eco:b1062.
    PATRICi 32117363. VBIEscCol129921_1104.

    Organism-specific databases

    EchoBASEi EB0799.
    EcoGenei EG10806. pyrC.

    Phylogenomic databases

    eggNOGi COG0418.
    HOGENOMi HOG000256259.
    KOi K01465.
    OMAi LKRNVHQ.
    OrthoDBi EOG6TFCMH.
    PhylomeDBi P05020.

    Enzyme and pathway databases

    UniPathwayi UPA00070 ; UER00117 .
    BioCyci EcoCyc:DIHYDROOROT-MONOMER.
    ECOL316407:JW1049-MONOMER.
    MetaCyc:DIHYDROOROT-MONOMER.
    BRENDAi 3.5.2.3. 2026.
    SABIO-RK P05020.

    Miscellaneous databases

    EvolutionaryTracei P05020.
    PROi P05020.

    Gene expression databases

    Genevestigatori P05020.

    Family and domain databases

    HAMAPi MF_00219. PyrC_type1.
    InterProi IPR006680. Amidohydro_1.
    IPR004721. DHOdimr.
    IPR002195. Dihydroorotase_CS.
    [Graphical view ]
    Pfami PF01979. Amidohydro_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001237. DHOdimr. 1 hit.
    TIGRFAMsi TIGR00856. pyrC_dimer. 1 hit.
    PROSITEi PS00482. DIHYDROOROTASE_1. 1 hit.
    PS00483. DIHYDROOROTASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the structural gene for dihydroorotase of Escherichia coli K12."
      Baeckstroem D., Sjoeberg R.-M., Lundberg L.G.
      Eur. J. Biochem. 160:77-82(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Nucleotide sequence and expression of the pyrC gene of Escherichia coli K-12."
      Wilson H.R., Chan P.T., Turnbough C.L. Jr.
      J. Bacteriol. 169:3051-3058(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. Ohmori H.
      Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 86-348.
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    7. "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12."
      Link A.J., Robison K., Church G.M.
      Electrophoresis 18:1259-1313(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-13.
      Strain: K12 / EMG2.
    8. "Dihydroorotase from Escherichia coli. Substitution of Co(II) for the active site Zn(II)."
      Brown D.C., Collins K.D.
      J. Biol. Chem. 266:1597-1604(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: ZINC-BINDING.
    9. "Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal center."
      Thoden J.B., Phillips G.N. Jr., Neal T.M., Raushel F.M., Holden H.M.
      Biochemistry 40:6989-6997(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS), CARBAMYLATION AT LYS-103.

    Entry informationi

    Entry nameiPYRC_ECOLI
    AccessioniPrimary (citable) accession number: P05020
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 142 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3