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P05017 (IGF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 145. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor I

Short name=IGF-I
Alternative name(s):
Somatomedin
Gene names
Name:Igf1
Synonyms:Igf-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length153 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation By similarity.

Subcellular location

Secreted.

Induction

Expression in the bone oscillates in a circadian manner and its expression is negatively regulated by CCRN4L/NOC. Ref.6

Sequence similarities

Belongs to the insulin family.

Sequence caution

The sequence AAH12409.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   Molecular functionGrowth factor
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processType I pneumocyte differentiation

Inferred from mutant phenotype PubMed 16691571. Source: MGI

Type II pneumocyte differentiation

Inferred from mutant phenotype PubMed 16691571. Source: MGI

blood vessel remodeling

Inferred from mutant phenotype PubMed 16691571. Source: MGI

branching morphogenesis of an epithelial tube

Inferred from mutant phenotype PubMed 15059953. Source: MGI

cell activation

Inferred from sequence orthology PubMed 22797923. Source: MGI

cell development

Inferred from mutant phenotype PubMed 10465256. Source: MGI

chondroitin sulfate proteoglycan biosynthetic process

Inferred from direct assay PubMed 17644814. Source: MGI

circadian rhythm

Inferred from expression pattern Ref.6. Source: UniProtKB

exocrine pancreas development

Inferred from mutant phenotype PubMed 12101187. Source: MGI

extrinsic apoptotic signaling pathway in absence of ligand

Inferred from direct assay PubMed 12431371. Source: MGI

glial cell differentiation

Inferred from mutant phenotype PubMed 12574418. Source: MGI

inner ear development

Inferred from direct assay PubMed 21795542. Source: MGI

insulin-like growth factor receptor signaling pathway

Inferred from direct assay PubMed 12805218. Source: MGI

lung alveolus development

Inferred from mutant phenotype PubMed 16691571. Source: MGI

lung development

Inferred from genetic interaction PubMed 16691571. Source: MGI

lung lobe morphogenesis

Inferred from mutant phenotype PubMed 16691571. Source: MGI

lung vasculature development

Inferred from mutant phenotype PubMed 16691571. Source: MGI

mammary gland development

Inferred from mutant phenotype PubMed 15059953. Source: MGI

multicellular organism growth

Inferred from direct assay PubMed 12865332. Source: MGI

negative regulation of ERK1 and ERK2 cascade

Inferred from genetic interaction PubMed 16691571. Source: MGI

negative regulation of androgen receptor signaling pathway

Inferred from mutant phenotype PubMed 18827067. Source: MGI

negative regulation of apoptotic process

Inferred from direct assay PubMed 18977203. Source: UniProtKB

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 16691571. Source: MGI

negative regulation of release of cytochrome c from mitochondria

Inferred from direct assay PubMed 18977203. Source: UniProtKB

nervous system development

Inferred from mutant phenotype PubMed 12574418. Source: MGI

osteoblast differentiation

Inferred from expression pattern PubMed 16311053. Source: BHF-UCL

positive regulation of cell proliferation

Inferred from genetic interaction PubMed 17636024. Source: MGI

positive regulation of cerebellar granule cell precursor proliferation

Inferred from direct assay PubMed 10027293. Source: MGI

positive regulation of glucose import

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of glycogen biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of myoblast proliferation

Inferred from mutant phenotype PubMed 21406500. Source: MGI

positive regulation of phosphatidylinositol 3-kinase signaling

Inferred from direct assay PubMed 21413931. Source: MGI

positive regulation of protein kinase B signaling

Inferred from direct assay PubMed 19208758. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15322112PubMed 17644814. Source: MGI

prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis

Inferred from mutant phenotype PubMed 10218945. Source: MGI

prostate gland epithelium morphogenesis

Inferred from mutant phenotype PubMed 17138659. Source: MGI

prostate gland growth

Inferred from direct assay PubMed 17138659. Source: MGI

prostate gland stromal morphogenesis

Inferred from mutant phenotype PubMed 17138659. Source: MGI

regulation of establishment or maintenance of cell polarity

Inferred from direct assay PubMed 7848824. Source: MGI

regulation of protein metabolic process

Inferred from genetic interaction PubMed 7510770. Source: MGI

regulation of protein phosphorylation

Inferred from direct assay PubMed 19744960. Source: MGI

water homeostasis

Inferred from genetic interaction PubMed 7510770. Source: MGI

   Cellular_componentextracellular space

Inferred from direct assay PubMed 17693256PubMed 7510770PubMed 9630227. Source: MGI

   Molecular_functioninsulin-like growth factor receptor binding

Inferred from physical interaction PubMed 14566968. Source: MGI

protein binding

Inferred from physical interaction PubMed 14566968PubMed 7679139. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform IGF-IA (identifier: P05017-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform IGF-IB (identifier: P05017-2)

The sequence of this isoform differs from the canonical sequence as follows:
     135-153: EVHLKNTSRGSAGNKTYRM → SPSLSTNKKTKLQRRRKGSTFEE

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – ? Potential
Propeptide? – 48 By similarityPRO_0000015666
Chain49 – 11870Insulin-like growth factor I
PRO_0000015667
Propeptide119 – 15335E peptide
PRO_0000015668

Regions

Region49 – 7729B
Region78 – 8912C
Region90 – 11021A
Region111 – 1188D

Amino acid modifications

Disulfide bond54 ↔ 96 By similarity
Disulfide bond66 ↔ 109 By similarity
Disulfide bond95 ↔ 100 By similarity

Natural variations

Alternative sequence135 – 15319EVHLK…KTYRM → SPSLSTNKKTKLQRRRKGST FEE in isoform IGF-IB.
VSP_012165

Sequences

Sequence LengthMass (Da)Tools
Isoform IGF-IA [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: 967596AEAC0CA387

FASTA15317,093
        10         20         30         40         50         60 
MGKISSLPTQ LFKICLCDFL KIKIHIMSSS HLFYLALCLL TFTSSTTAGP ETLCGAELVD 

        70         80         90        100        110        120 
ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKAARS 

       130        140        150 
IRAQRHTDMP KTQKEVHLKN TSRGSAGNKT YRM 

« Hide

Isoform IGF-IB [UniParc].

Checksum: 153C6311A323ACA4
Show »

FASTA15717,623

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IGF-IA).
Strain: C57BL/6J.
Tissue: Cerebellum.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-153 (ISOFORM IGF-IB).
Strain: FVB/N.
Tissue: Liver.
[3]"Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors."
Bell G.I., Stempien M.M., Fong N.M., Rall L.B.
Nucleic Acids Res. 14:7873-7882(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-153 (ISOFORMS IGF-IA AND IGF-IB).
Tissue: Liver.
[4]"Insulin-like growth factors (IGF) in muscle development. Expression of IGF-I, the IGF-I receptor, and an IGF binding protein during myoblast differentiation."
Tollefsen S.E., Lajara R., McCusker R.H., Clemmons D.R., Rotwein P.
J. Biol. Chem. 264:13810-13817(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-134.
[5]"Regulation of insulin-like growth factor I gene expression by growth hormone."
Mathews L.S., Norstedt G., Palmiter R.D.
Proc. Natl. Acad. Sci. U.S.A. 83:9343-9347(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-134.
[6]"Nocturnin suppresses igf1 expression in bone by targeting the 3' untranslated region of igf1 mRNA."
Kawai M., Delany A.M., Green C.B., Adamo M.L., Rosen C.J.
Endocrinology 151:4861-4870(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK081019 mRNA. Translation: BAC38117.1.
BC012409 mRNA. Translation: AAH12409.1. Different initiation.
X04480 mRNA. Translation: CAA28168.1.
X04482 mRNA. Translation: CAA28170.1.
M28139 Genomic DNA. Translation: AAA74553.1.
M14983 Genomic DNA. Translation: AAA37925.1.
CCDSCCDS48661.1. [P05017-1]
PIRA25540.
RefSeqNP_001104745.1. NM_001111275.1. [P05017-1]
NP_034642.2. NM_010512.4.
XP_006513326.1. XM_006513263.1. [P05017-1]
XP_006513329.1. XM_006513266.1.
UniGeneMm.268521.

3D structure databases

ProteinModelPortalP05017.
SMRP05017. Positions 50-111.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-266530.
STRING10090.ENSMUSP00000056668.

PTM databases

PhosphoSiteP05017.

Proteomic databases

PaxDbP05017.
PRIDEP05017.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000095360; ENSMUSP00000093005; ENSMUSG00000020053. [P05017-1]
ENSMUST00000122386; ENSMUSP00000113905; ENSMUSG00000020053. [P05017-1]
GeneID16000.
KEGGmmu:16000.
UCSCuc007gqw.2. mouse. [P05017-1]

Organism-specific databases

CTD3479.
MGIMGI:96432. Igf1.

Phylogenomic databases

eggNOGNOG39886.
HOGENOMHOG000233362.
HOVERGENHBG006137.
KOK05459.
OrthoDBEOG7TF7CG.

Gene expression databases

ArrayExpressP05017.
BgeeP05017.
CleanExMM_IGF1.
GenevestigatorP05017.

Family and domain databases

Gene3D1.10.100.10. 1 hit.
InterProIPR022341. IGF-I.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF00049. Insulin. 2 hits.
[Graphical view]
PRINTSPR02002. INSLNLIKEGF.
PR02005. INSLNLIKEGF1.
PR00276. INSULINFAMLY.
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 1 hit.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIGF1. mouse.
NextBio288784.
PROP05017.
SOURCESearch...

Entry information

Entry nameIGF1_MOUSE
AccessionPrimary (citable) accession number: P05017
Secondary accession number(s): P05018, Q6LDP4, Q8C4U6
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 7, 2004
Last modified: July 9, 2014
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot