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Protein

Insulin-like growth factor I

Gene

Igf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (By similarity).By similarity

GO - Molecular functioni

  1. hormone activity Source: MGI
  2. insulin-like growth factor receptor binding Source: MGI
  3. insulin receptor binding Source: MGI
  4. integrin binding Source: MGI

GO - Biological processi

  1. blood vessel remodeling Source: MGI
  2. bone mineralization involved in bone maturation Source: MGI
  3. branching morphogenesis of an epithelial tube Source: MGI
  4. cell activation Source: MGI
  5. cell development Source: MGI
  6. chondroitin sulfate proteoglycan biosynthetic process Source: MGI
  7. circadian rhythm Source: UniProtKB
  8. exocrine pancreas development Source: MGI
  9. extrinsic apoptotic signaling pathway in absence of ligand Source: MGI
  10. glial cell differentiation Source: MGI
  11. inner ear development Source: MGI
  12. insulin-like growth factor receptor signaling pathway Source: MGI
  13. lung alveolus development Source: MGI
  14. lung development Source: MGI
  15. lung lobe morphogenesis Source: MGI
  16. lung vasculature development Source: MGI
  17. mammary gland development Source: MGI
  18. multicellular organism growth Source: MGI
  19. muscle hypertrophy Source: MGI
  20. myoblast differentiation Source: MGI
  21. myoblast proliferation Source: MGI
  22. myotube cell development Source: MGI
  23. negative regulation of androgen receptor signaling pathway Source: MGI
  24. negative regulation of apoptotic process Source: UniProtKB
  25. negative regulation of cell proliferation Source: MGI
  26. negative regulation of ERK1 and ERK2 cascade Source: MGI
  27. negative regulation of extrinsic apoptotic signaling pathway Source: MGI
  28. negative regulation of oocyte development Source: MGI
  29. negative regulation of release of cytochrome c from mitochondria Source: UniProtKB
  30. negative regulation of smooth muscle cell apoptotic process Source: MGI
  31. nervous system development Source: MGI
  32. osteoblast differentiation Source: BHF-UCL
  33. phosphatidylinositol-mediated signaling Source: MGI
  34. positive regulation of activated T cell proliferation Source: MGI
  35. positive regulation of calcineurin-NFAT signaling cascade Source: MGI
  36. positive regulation of cardiac muscle hypertrophy Source: MGI
  37. positive regulation of cell proliferation Source: MGI
  38. positive regulation of cerebellar granule cell precursor proliferation Source: MGI
  39. positive regulation of DNA binding Source: MGI
  40. positive regulation of DNA replication Source: MGI
  41. positive regulation of epithelial cell proliferation Source: MGI
  42. positive regulation of fibroblast proliferation Source: MGI
  43. positive regulation of glucose import Source: UniProtKB
  44. positive regulation of glycogen biosynthetic process Source: UniProtKB
  45. positive regulation of glycolytic process Source: MGI
  46. positive regulation of insulin-like growth factor receptor signaling pathway Source: MGI
  47. positive regulation of MAPK cascade Source: MGI
  48. positive regulation of mitotic nuclear division Source: MGI
  49. positive regulation of myoblast proliferation Source: MGI
  50. positive regulation of osteoblast differentiation Source: MGI
  51. positive regulation of peptidyl-tyrosine phosphorylation Source: MGI
  52. positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  53. positive regulation of protein import into nucleus, translocation Source: MGI
  54. positive regulation of protein kinase B signaling Source: MGI
  55. positive regulation of Ras protein signal transduction Source: MGI
  56. positive regulation of smooth muscle cell migration Source: MGI
  57. positive regulation of smooth muscle cell proliferation Source: MGI
  58. positive regulation of transcription, DNA-templated Source: MGI
  59. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  60. positive regulation of tyrosine phosphorylation of Stat5 protein Source: MGI
  61. prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis Source: MGI
  62. prostate gland epithelium morphogenesis Source: MGI
  63. prostate gland growth Source: MGI
  64. prostate gland stromal morphogenesis Source: MGI
  65. proteoglycan biosynthetic process Source: MGI
  66. regulation of establishment or maintenance of cell polarity Source: MGI
  67. regulation of gene expression Source: MGI
  68. regulation of protein metabolic process Source: MGI
  69. regulation of protein phosphorylation Source: MGI
  70. response to heat Source: MGI
  71. skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration Source: MGI
  72. Type II pneumocyte differentiation Source: MGI
  73. Type I pneumocyte differentiation Source: MGI
  74. water homeostasis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Growth factor

Enzyme and pathway databases

ReactomeiREACT_282332. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_307071. Platelet degranulation.
REACT_321558. SHC-related events triggered by IGF1R.
REACT_330516. Synthesis, secretion, and deacylation of Ghrelin.
REACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_346521. IRS-related events triggered by IGF1R.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor I
Short name:
IGF-I
Alternative name(s):
Somatomedin
Gene namesi
Name:Igf1
Synonyms:Igf-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:96432. Igf1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei? – 48By similarityPRO_0000015666
Signal peptidei1 – ?Sequence Analysis
Chaini49 – 11870Insulin-like growth factor IPRO_0000015667Add
BLAST
Propeptidei119 – 15335E peptidePRO_0000015668Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 96By similarity
Disulfide bondi66 ↔ 109By similarity
Disulfide bondi95 ↔ 100By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiP05017.
PaxDbiP05017.
PRIDEiP05017.

PTM databases

PhosphoSiteiP05017.

Expressioni

Inductioni

Expression in the bone oscillates in a circadian manner and its expression is negatively regulated by CCRN4L/NOC.1 Publication

Gene expression databases

BgeeiP05017.
CleanExiMM_IGF1.
ExpressionAtlasiP05017. baseline and differential.
GenevestigatoriP05017.

Interactioni

Protein-protein interaction databases

MINTiMINT-266530.
STRINGi10090.ENSMUSP00000056668.

Structurei

3D structure databases

ProteinModelPortaliP05017.
SMRiP05017. Positions 50-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 7729BAdd
BLAST
Regioni78 – 8912CAdd
BLAST
Regioni90 – 11021AAdd
BLAST
Regioni111 – 1188D

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG39886.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP05017.
KOiK05459.
OrthoDBiEOG7TF7CG.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022341. IGF-I.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 2 hits.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02005. INSLNLIKEGF1.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform IGF-IA (identifier: P05017-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKISSLPTQ LFKICLCDFL KIKIHIMSSS HLFYLALCLL TFTSSTTAGP
60 70 80 90 100
ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC
110 120 130 140 150
DLRRLEMYCA PLKPTKAARS IRAQRHTDMP KTQKEVHLKN TSRGSAGNKT

YRM
Length:153
Mass (Da):17,093
Last modified:December 7, 2004 - v2
Checksum:i967596AEAC0CA387
GO
Isoform IGF-IB (identifier: P05017-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     135-153: EVHLKNTSRGSAGNKTYRM → SPSLSTNKKTKLQRRRKGSTFEE

Show »
Length:157
Mass (Da):17,623
Checksum:i153C6311A323ACA4
GO

Sequence cautioni

The sequence AAH12409.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei135 – 15319EVHLK…KTYRM → SPSLSTNKKTKLQRRRKGST FEE in isoform IGF-IB. 2 PublicationsVSP_012165Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK081019 mRNA. Translation: BAC38117.1.
BC012409 mRNA. Translation: AAH12409.1. Different initiation.
X04480 mRNA. Translation: CAA28168.1.
X04482 mRNA. Translation: CAA28170.1.
M28139 Genomic DNA. Translation: AAA74553.1.
M14983 Genomic DNA. Translation: AAA37925.1.
CCDSiCCDS48661.1. [P05017-1]
PIRiA25540.
RefSeqiNP_001104745.1. NM_001111275.1. [P05017-1]
NP_034642.2. NM_010512.4.
XP_006513326.1. XM_006513263.1. [P05017-1]
XP_006513329.1. XM_006513266.2.
UniGeneiMm.268521.

Genome annotation databases

EnsembliENSMUST00000095360; ENSMUSP00000093005; ENSMUSG00000020053. [P05017-1]
ENSMUST00000122386; ENSMUSP00000113905; ENSMUSG00000020053. [P05017-1]
GeneIDi16000.
KEGGimmu:16000.
UCSCiuc007gqw.2. mouse. [P05017-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK081019 mRNA. Translation: BAC38117.1.
BC012409 mRNA. Translation: AAH12409.1. Different initiation.
X04480 mRNA. Translation: CAA28168.1.
X04482 mRNA. Translation: CAA28170.1.
M28139 Genomic DNA. Translation: AAA74553.1.
M14983 Genomic DNA. Translation: AAA37925.1.
CCDSiCCDS48661.1. [P05017-1]
PIRiA25540.
RefSeqiNP_001104745.1. NM_001111275.1. [P05017-1]
NP_034642.2. NM_010512.4.
XP_006513326.1. XM_006513263.1. [P05017-1]
XP_006513329.1. XM_006513266.2.
UniGeneiMm.268521.

3D structure databases

ProteinModelPortaliP05017.
SMRiP05017. Positions 50-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-266530.
STRINGi10090.ENSMUSP00000056668.

PTM databases

PhosphoSiteiP05017.

Proteomic databases

MaxQBiP05017.
PaxDbiP05017.
PRIDEiP05017.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095360; ENSMUSP00000093005; ENSMUSG00000020053. [P05017-1]
ENSMUST00000122386; ENSMUSP00000113905; ENSMUSG00000020053. [P05017-1]
GeneIDi16000.
KEGGimmu:16000.
UCSCiuc007gqw.2. mouse. [P05017-1]

Organism-specific databases

CTDi3479.
MGIiMGI:96432. Igf1.

Phylogenomic databases

eggNOGiNOG39886.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP05017.
KOiK05459.
OrthoDBiEOG7TF7CG.

Enzyme and pathway databases

ReactomeiREACT_282332. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_307071. Platelet degranulation.
REACT_321558. SHC-related events triggered by IGF1R.
REACT_330516. Synthesis, secretion, and deacylation of Ghrelin.
REACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_346521. IRS-related events triggered by IGF1R.

Miscellaneous databases

ChiTaRSiIgf1. mouse.
NextBioi288784.
PROiP05017.
SOURCEiSearch...

Gene expression databases

BgeeiP05017.
CleanExiMM_IGF1.
ExpressionAtlasiP05017. baseline and differential.
GenevestigatoriP05017.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022341. IGF-I.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF00049. Insulin. 2 hits.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02005. INSLNLIKEGF1.
PR00276. INSULINFAMLY.
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM IGF-IA).
    Strain: C57BL/6J.
    Tissue: Cerebellum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-153 (ISOFORM IGF-IB).
    Strain: FVB/N.
    Tissue: Liver.
  3. "Sequences of liver cDNAs encoding two different mouse insulin-like growth factor I precursors."
    Bell G.I., Stempien M.M., Fong N.M., Rall L.B.
    Nucleic Acids Res. 14:7873-7882(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 27-153 (ISOFORMS IGF-IA AND IGF-IB).
    Tissue: Liver.
  4. "Insulin-like growth factors (IGF) in muscle development. Expression of IGF-I, the IGF-I receptor, and an IGF binding protein during myoblast differentiation."
    Tollefsen S.E., Lajara R., McCusker R.H., Clemmons D.R., Rotwein P.
    J. Biol. Chem. 264:13810-13817(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-134.
  5. "Regulation of insulin-like growth factor I gene expression by growth hormone."
    Mathews L.S., Norstedt G., Palmiter R.D.
    Proc. Natl. Acad. Sci. U.S.A. 83:9343-9347(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 75-134.
  6. "Nocturnin suppresses igf1 expression in bone by targeting the 3' untranslated region of igf1 mRNA."
    Kawai M., Delany A.M., Green C.B., Adamo M.L., Rosen C.J.
    Endocrinology 151:4861-4870(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiIGF1_MOUSE
AccessioniPrimary (citable) accession number: P05017
Secondary accession number(s): P05018, Q6LDP4, Q8C4U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 7, 2004
Last modified: April 1, 2015
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.