P05014 (IFNA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interferon alpha-4
Short name=IFN-alpha-4

Alternative name(s):
Interferon alpha-4B
Interferon alpha-76
Interferon alpha-M1

Gene names

Name:

IFNA4

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Subcellular location	Secreted.
Polymorphism	Two forms exist; alpha-4a (shown here) and alpha-4b. They seem to be equally abundant.
Sequence similarities	Belongs to the alpha/beta interferon family.

Ontologies

Keywords
Biological process	Antiviral defense
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	blood coagulation Traceable author statement. Source: Reactome defense response to virus Inferred from electronic annotation. Source: UniProtKB-KW regulation of type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome response to virus Traceable author statement PubMed 3891272. Source: ProtInc type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular_component	extracellular region Traceable author statement. Source: Reactome extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	interferon-alpha/beta receptor binding Traceable author statement PubMed 3891272. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.6
	Chain	24 – 189	166	Interferon alpha-4		PRO_0000016361
Amino acid modifications
	Disulfide bond	24 ↔ 122		By similarity
	Disulfide bond	52 ↔ 162		By similarity
Natural variations
	Natural variant	49	1	H → P. Corresponds to variant rs3203573 [ dbSNP | Ensembl ].		VAR_034010
	Natural variant	74	1	A → T in alpha-4B. Ref.2 Ref.4 Ref.5 Corresponds to variant rs1062571 [ dbSNP | Ensembl ].		VAR_013002
	Natural variant	137	1	E → V in alpha-4B. Ref.2 Corresponds to variant rs3750480 [ dbSNP | Ensembl ].		VAR_013003

Sequences

	Sequence		Length	Mass (Da)
	P05014 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 828DF9C33ABC337F	FASTA	189	21,808
	10 20 30 40 50 60 MALSFSLLMA VLVLSYKSIC SLGCDLPQTH SLGNRRALIL LAQMGRISHF SCLKDRHDFG 70 80 90 100 110 120 FPEEEFDGHQ FQKAQAISVL HEMIQQTFNL FSTEDSSAAW EQSLLEKFST ELYQQLNDLE 130 140 150 160 170 180 ACVIQEVGVE ETPLMNEDSI LAVRKYFQRI TLYLTEKKYS PCAWEVVRAE IMRSLSFSTN LQKRLRRKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and expression in E. coli of a human interferon-alpha gene selected from a genomic library using synthetic oligonucleotides." Linnane A.W., Beilharz M.W., McMullen G.L., Macreadie I.G., Murphy M., Nisbet I.T., Novitski C.E., Woodrow G.C. Biochem. Int. 8:725-732(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structural relationship of human interferon alpha genes and pseudogenes." Henco K., Brosius J., Fujisawa A., Fujisawa J., Haynes J.R., Hochstadt J., Kovacic T., Pasek M., Schamboeck A., Schmid J., Todokoro K., Waelchli M., Nagata S., Weissmann C. J. Mol. Biol. 185:227-260(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALPHA-4B THR-74 AND VAL-137.
[3]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-74.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-74. Tissue: Brain.
[6]	"Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes." Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N. Biochem. J. 329:295-302(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 24-56.
[7]	"Both variant forms of interferon-alpha4 gene (IFNA4a and IFNA4b) are present in the human population." Hussain M., Gill D.S., Liao M.-J. J. Interferon Cytokine Res. 17:559-566(1997) [PubMed] [Europe PMC] [Abstract] Cited for: POLYMORPHISM.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M27318 mRNA. Translation: AAA52726.1. X02955 Genomic DNA. Translation: CAA26701.1. AL512606 Genomic DNA. Translation: CAH71188.1. CH471071 Genomic DNA. Translation: EAW58621.1. BC074965 mRNA. Translation: AAH74965.1. BC074966 mRNA. Translation: AAH74966.1. BC113640 mRNA. Translation: AAI13641.1. BC113642 mRNA. Translation: AAI13643.1.
IPI	IPI00291465.
PIR	IVHU4B. E23753. I52347.
RefSeq	NP_066546.1. NM_021068.2.
UniGene	Hs.1510.
3D structure databases
ProteinModelPortal	P05014.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000412897.
PTM databases
PhosphoSite	P05014.
Polymorphism databases
DMDM	84029375.
Proteomic databases
PaxDb	P05014.
PRIDE	P05014.
Protocols and materials databases
DNASU	3441.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000421715; ENSP00000412897; ENSG00000236637.
GeneID	3441.
KEGG	hsa:3441.
UCSC	uc003zon.2. human.
Organism-specific databases
CTD	3441.
GeneCards	GC09M021186.
HGNC	HGNC:5425. IFNA4.
HPA	HPA047557.
MIM	147564. gene.
neXtProt	NX_P05014.
PharmGKB	PA29664.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG119628.
HOGENOM	HOG000230500.
HOVERGEN	HBG052086.
InParanoid	P05014.
KO	K05414.
OMA	EYFHRIS.
OrthoDB	EOG4KKZ45.
PhylomeDB	P05014.
Enzyme and pathway databases
Pathway_Interaction_DB	cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.
Reactome	REACT_604. Hemostasis. REACT_6900. Immune System.
Gene expression databases
ArrayExpress	P05014.
CleanEx	HS_IFNA4.
Genevestigator	P05014.
GermOnline	ENSG00000147877. Homo sapiens.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR015589. Interferon_alpha. IPR000471. Interferon_alpha/beta/delta. [Graphical view]
PANTHER	PTHR11691. PTHR11691. 1 hit. PTHR11691:SF9. PTHR11691:SF9. 1 hit.
Pfam	PF00143. Interferon. 1 hit. [Graphical view]
PRINTS	PR00266. INTERFERONAB.
SMART	SM00076. IFabd. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00252. INTERFERON_A_B_D. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	3441.
NextBio	13560.
SOURCE	Search...

Entry information

Entry name

IFNA4_HUMAN

Accession

Primary (citable) accession number: P05014
Secondary accession number(s): P13358, Q14CS4, Q5VV15

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	May 10, 2005
Last modified:	May 1, 2013
This is version 131 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.