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P05014 (IFNA4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon alpha-4

Short name=IFN-alpha-4
Alternative name(s):
Interferon alpha-4B
Interferon alpha-76
Interferon alpha-M1
Gene names
Name:IFNA4
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.

Subcellular location

Secreted.

Polymorphism

Two forms exist; alpha-4a (shown here) and alpha-4b. They seem to be equally abundant.

Sequence similarities

Belongs to the alpha/beta interferon family.

Ontologies

Keywords
   Biological processAntiviral defense
   Cellular componentSecreted
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionCytokine
   PTMDisulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processB cell differentiation

Inferred from Biological aspect of Ancestor. Source: RefGenome

B cell proliferation

Inferred from Biological aspect of Ancestor. Source: RefGenome

T cell activation involved in immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

adaptive immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

blood coagulation

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

defense response to virus

Inferred from electronic annotation. Source: UniProtKB-KW

humoral immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

innate immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

natural killer cell activation involved in immune response

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of peptidyl-serine phosphorylation of STAT protein

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of MHC class I biosynthetic process

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of type I interferon-mediated signaling pathway

Traceable author statement. Source: Reactome

response to exogenous dsRNA

Inferred from Biological aspect of Ancestor. Source: RefGenome

response to virus

Traceable author statement PubMed 3891272. Source: ProtInc

type I interferon signaling pathway

Traceable author statement. Source: Reactome

   Cellular_componentextracellular region

Traceable author statement. Source: Reactome

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functioncytokine activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

type I interferon receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Ref.6
Chain24 – 189166Interferon alpha-4
PRO_0000016361

Amino acid modifications

Disulfide bond24 ↔ 122 By similarity
Disulfide bond52 ↔ 162 By similarity

Natural variations

Natural variant491H → P.
Corresponds to variant rs3203573 [ dbSNP | Ensembl ].
VAR_034010
Natural variant741A → T in alpha-4B. Ref.2 Ref.4 Ref.5
Corresponds to variant rs1062571 [ dbSNP | Ensembl ].
VAR_013002
Natural variant1371E → V in alpha-4B. Ref.2
Corresponds to variant rs3750480 [ dbSNP | Ensembl ].
VAR_013003

Sequences

Sequence LengthMass (Da)Tools
P05014 [UniParc].

Last modified May 10, 2005. Version 2.
Checksum: 828DF9C33ABC337F

FASTA18921,808
        10         20         30         40         50         60 
MALSFSLLMA VLVLSYKSIC SLGCDLPQTH SLGNRRALIL LAQMGRISHF SCLKDRHDFG 

        70         80         90        100        110        120 
FPEEEFDGHQ FQKAQAISVL HEMIQQTFNL FSTEDSSAAW EQSLLEKFST ELYQQLNDLE 

       130        140        150        160        170        180 
ACVIQEVGVE ETPLMNEDSI LAVRKYFQRI TLYLTEKKYS PCAWEVVRAE IMRSLSFSTN 


LQKRLRRKD 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and expression in E. coli of a human interferon-alpha gene selected from a genomic library using synthetic oligonucleotides."
Linnane A.W., Beilharz M.W., McMullen G.L., Macreadie I.G., Murphy M., Nisbet I.T., Novitski C.E., Woodrow G.C.
Biochem. Int. 8:725-732(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structural relationship of human interferon alpha genes and pseudogenes."
Henco K., Brosius J., Fujisawa A., Fujisawa J., Haynes J.R., Hochstadt J., Kovacic T., Pasek M., Schamboeck A., Schmid J., Todokoro K., Waelchli M., Nagata S., Weissmann C.
J. Mol. Biol. 185:227-260(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALPHA-4B THR-74 AND VAL-137.
[3]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT THR-74.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT THR-74.
Tissue: Brain.
[6]"Identification of nine interferon-alpha subtypes produced by Sendai virus-induced human peripheral blood leucocytes."
Nyman T.A., Toeloe H., Parkkinen J., Kalkkinen N.
Biochem. J. 329:295-302(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 24-56.
[7]"Both variant forms of interferon-alpha4 gene (IFNA4a and IFNA4b) are present in the human population."
Hussain M., Gill D.S., Liao M.-J.
J. Interferon Cytokine Res. 17:559-566(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: POLYMORPHISM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27318 mRNA. Translation: AAA52726.1.
X02955 Genomic DNA. Translation: CAA26701.1.
AL512606 Genomic DNA. Translation: CAH71188.1.
CH471071 Genomic DNA. Translation: EAW58621.1.
BC074965 mRNA. Translation: AAH74965.1.
BC074966 mRNA. Translation: AAH74966.1.
BC113640 mRNA. Translation: AAI13641.1.
BC113642 mRNA. Translation: AAI13643.1.
PIRIVHU4B. E23753.
I52347.
RefSeqNP_066546.1. NM_021068.2.
UniGeneHs.1510.

3D structure databases

ProteinModelPortalP05014.
SMRP05014. Positions 24-189.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000412897.

PTM databases

PhosphoSiteP05014.

Polymorphism databases

DMDM84029375.

Proteomic databases

PaxDbP05014.
PRIDEP05014.

Protocols and materials databases

DNASU3441.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000421715; ENSP00000412897; ENSG00000236637.
GeneID3441.
KEGGhsa:3441.
UCSCuc003zon.2. human.

Organism-specific databases

CTD3441.
GeneCardsGC09M021186.
HGNCHGNC:5425. IFNA4.
HPAHPA047557.
MIM147564. gene.
neXtProtNX_P05014.
PharmGKBPA29664.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG119628.
HOGENOMHOG000230500.
HOVERGENHBG052086.
InParanoidP05014.
KOK05414.
OMAYFERITI.
OrthoDBEOG7M98HV.
PhylomeDBP05014.
TreeFamTF336177.

Enzyme and pathway databases

ReactomeREACT_604. Hemostasis.
REACT_6900. Immune System.

Gene expression databases

CleanExHS_IFNA4.
GenevestigatorP05014.

Family and domain databases

Gene3D1.20.1250.10. 1 hit.
InterProIPR009079. 4_helix_cytokine-like_core.
IPR012351. 4_helix_cytokine_core.
IPR015589. Interferon_alpha.
IPR000471. Interferon_alpha/beta/delta.
[Graphical view]
PANTHERPTHR11691. PTHR11691. 1 hit.
PTHR11691:SF9. PTHR11691:SF9. 1 hit.
PfamPF00143. Interferon. 1 hit.
[Graphical view]
PRINTSPR00266. INTERFERONAB.
SMARTSM00076. IFabd. 1 hit.
[Graphical view]
SUPFAMSSF47266. SSF47266. 1 hit.
PROSITEPS00252. INTERFERON_A_B_D. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiIFNA4.
GenomeRNAi3441.
NextBio13560.
PROP05014.
SOURCESearch...

Entry information

Entry nameIFNA4_HUMAN
AccessionPrimary (citable) accession number: P05014
Secondary accession number(s): P13358, Q14CS4, Q5VV15
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM