ID   IFNW1_HUMAN             Reviewed;         195 AA.
AC   P05000; Q13168; Q5U802; Q5VWD0; Q7M4P5;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   27-MAR-2024, entry version 198.
DE   RecName: Full=Interferon omega-1;
DE   AltName: Full=Interferon alpha-II-1;
DE   Flags: Precursor;
GN   Name=IFNW1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2985969; DOI=10.1128/mcb.5.4.768-779.1985;
RA   Capon D.J., Shepard H.M., Goeddel D.V.;
RT   "Two distinct families of human and bovine interferon-alpha genes are
RT   coordinately expressed and encode functional polypeptides.";
RL   Mol. Cell. Biol. 5:768-779(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Leukocyte;
RX   PubMed=1647209; DOI=10.1016/0167-4781(91)90004-6;
RA   Adolf G.R., Fruehbeis B., Hauptmann R., Kalsner I., Maurer-Fogy I.,
RA   Ostermann E., Patzelt E., Schwendenwein R., Sommergruber W., Zoephel A.;
RT   "Human interferon omega 1: isolation of the gene, expression in Chinese
RT   hamster ovary cells and characterization of the recombinant protein.";
RL   Biochim. Biophys. Acta 1089:167-174(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15164053; DOI=10.1038/nature02465;
RA   Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA   Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA   Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA   Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA   Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA   Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA   Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA   Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA   Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA   Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA   Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA   Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA   Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA   Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA   Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA   Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA   Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA   Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA   McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA   Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA   Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA   Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA   Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA   West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA   Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA   Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA   Dunham I.;
RT   "DNA sequence and analysis of human chromosome 9.";
RL   Nature 429:369-374(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 22-195.
RX   PubMed=3895159; DOI=10.1093/nar/13.13.4739;
RA   Hauptmann R., Swetly P.;
RT   "A novel class of human type I interferons.";
RL   Nucleic Acids Res. 13:4739-4749(1985).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 22-195.
RX   PubMed=8142025;
RA   Li M.F., Zeng Q., Zhou Y., Guo H.Y., Hou Y.D.;
RT   "Cloning, sequencing and expression in E. coli of interferon-omega 1
RT   gene.";
RL   Sci. China, Ser. B, Chem. Life Sci. Earth Sci. 36:1361-1366(1993).
RN   [8]
RP   PROTEIN SEQUENCE OF 22-55.
RX   PubMed=7765487; DOI=10.1271/bbb.58.1714;
RA   Shirono H., Koga J., Uemura H., Matsuo A.;
RT   "Identification of glycosylated subtypes of interferon-alpha produced by
RT   human leukocytes.";
RL   Biosci. Biotechnol. Biochem. 58:1714-1715(1994).
RN   [9]
RP   PROTEIN SEQUENCE OF 22-36.
RX   PubMed=15340161; DOI=10.1110/ps.04682504;
RA   Zhang Z., Henzel W.J.;
RT   "Signal peptide prediction based on analysis of experimentally verified
RT   cleavage sites.";
RL   Protein Sci. 13:2819-2824(2004).
RN   [10]
RP   PROTEIN SEQUENCE OF 22-33, AND GLYCOSYLATION AT ASN-101.
RX   PubMed=1693148; DOI=10.1016/s0021-9258(19)38846-5;
RA   Adolf G.R., Maurer-Fogy I., Kalsner I., Cantell K.;
RT   "Purification and characterization of natural human interferon omega 1. Two
RT   alternative cleavage sites for the signal peptidase.";
RL   J. Biol. Chem. 265:9290-9295(1990).
RN   [11]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 24-195.
RC   TISSUE=Liver;
RA   Yang R.Y., Yang Z.F., Zhu Y.T., Feng L.L., Liu N., Zhao F., Zhang F.X.,
RA   Fu L.C.;
RT   "Human IFN-omega 1 gene isolated from embryonic hepar.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [12]
RP   X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 22-195 IN COMPLEX WITH IFNAR1 AND
RP   IFNAR2, AND DISULFIDE BOND.
RX   PubMed=21854986; DOI=10.1016/j.cell.2011.06.048;
RA   Thomas C., Moraga I., Levin D., Krutzik P.O., Podoplelova Y., Trejo A.,
RA   Lee C., Yarden G., Vleck S.E., Glenn J.S., Nolan G.P., Piehler J.,
RA   Schreiber G., Garcia K.C.;
RT   "Structural linkage between ligand discrimination and receptor activation
RT   by type I interferons.";
RL   Cell 146:621-632(2011).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the alpha/beta interferon family. {ECO:0000305}.
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DR   EMBL; M11003; AAA52724.1; -; Genomic_DNA.
DR   EMBL; X58822; CAA41626.1; -; Genomic_DNA.
DR   EMBL; AL390882; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471071; EAW58624.1; -; Genomic_DNA.
DR   EMBL; BC069095; AAH69095.1; -; mRNA.
DR   EMBL; BC117290; AAI17291.1; -; mRNA.
DR   EMBL; BC117292; AAI17293.1; -; mRNA.
DR   EMBL; X02669; CAA26501.1; -; mRNA.
DR   EMBL; U25670; AAA70091.1; -; Genomic_DNA.
DR   EMBL; AY780805; AAV49320.1; -; mRNA.
DR   CCDS; CCDS6496.1; -.
DR   PIR; A93070; IVHUII.
DR   PIR; PC2204; PC2204.
DR   PIR; PC2205; PC2205.
DR   RefSeq; NP_002168.1; NM_002177.2.
DR   PDB; 3SE4; X-ray; 3.50 A; B=22-195.
DR   PDBsum; 3SE4; -.
DR   AlphaFoldDB; P05000; -.
DR   SMR; P05000; -.
DR   BioGRID; 109689; 7.
DR   ComplexPortal; CPX-6010; Interferon omega receptor-ligand complex.
DR   CORUM; P05000; -.
DR   IntAct; P05000; 3.
DR   STRING; 9606.ENSP00000369578; -.
DR   GlyConnect; 295; 2 N-Linked glycans (1 site).
DR   GlyCosmos; P05000; 1 site, 4 glycans.
DR   GlyGen; P05000; 1 site, 4 N-linked glycans (1 site).
DR   iPTMnet; P05000; -.
DR   BioMuta; IFNW1; -.
DR   DMDM; 400061; -.
DR   MassIVE; P05000; -.
DR   PaxDb; 9606-ENSP00000369578; -.
DR   PeptideAtlas; P05000; -.
DR   ProteomicsDB; 51761; -.
DR   Antibodypedia; 24850; 159 antibodies from 21 providers.
DR   DNASU; 3467; -.
DR   Ensembl; ENST00000380229.4; ENSP00000369578.2; ENSG00000177047.7.
DR   GeneID; 3467; -.
DR   KEGG; hsa:3467; -.
DR   MANE-Select; ENST00000380229.4; ENSP00000369578.2; NM_002177.3; NP_002168.1.
DR   UCSC; uc003zol.2; human.
DR   AGR; HGNC:5448; -.
DR   CTD; 3467; -.
DR   DisGeNET; 3467; -.
DR   GeneCards; IFNW1; -.
DR   HGNC; HGNC:5448; IFNW1.
DR   HPA; ENSG00000177047; Not detected.
DR   MIM; 147553; gene.
DR   neXtProt; NX_P05000; -.
DR   OpenTargets; ENSG00000177047; -.
DR   PharmGKB; PA29683; -.
DR   VEuPathDB; HostDB:ENSG00000177047; -.
DR   eggNOG; ENOG502T289; Eukaryota.
DR   GeneTree; ENSGT01000000214430; -.
DR   HOGENOM; CLU_109427_0_0_1; -.
DR   InParanoid; P05000; -.
DR   OMA; VVRMEIM; -.
DR   OrthoDB; 5358931at2759; -.
DR   PhylomeDB; P05000; -.
DR   TreeFam; TF336177; -.
DR   PathwayCommons; P05000; -.
DR   SignaLink; P05000; -.
DR   SIGNOR; P05000; -.
DR   BioGRID-ORCS; 3467; 23 hits in 1137 CRISPR screens.
DR   GeneWiki; IFNW1; -.
DR   GenomeRNAi; 3467; -.
DR   Pharos; P05000; Tbio.
DR   PRO; PR:P05000; -.
DR   Proteomes; UP000005640; Chromosome 9.
DR   RNAct; P05000; Protein.
DR   Bgee; ENSG00000177047; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 2 other cell types or tissues.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR   GO; GO:0005126; F:cytokine receptor binding; TAS:ProtInc.
DR   GO; GO:0005132; F:type I interferon receptor binding; IBA:GO_Central.
DR   GO; GO:0002250; P:adaptive immune response; IBA:GO_Central.
DR   GO; GO:0030183; P:B cell differentiation; IBA:GO_Central.
DR   GO; GO:0042100; P:B cell proliferation; IBA:GO_Central.
DR   GO; GO:0098586; P:cellular response to virus; NAS:ComplexPortal.
DR   GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0006959; P:humoral immune response; IBA:GO_Central.
DR   GO; GO:0002323; P:natural killer cell activation involved in immune response; IBA:GO_Central.
DR   GO; GO:0033141; P:positive regulation of peptidyl-serine phosphorylation of STAT protein; IBA:GO_Central.
DR   GO; GO:0051726; P:regulation of cell cycle; TAS:ProtInc.
DR   GO; GO:0043330; P:response to exogenous dsRNA; IBA:GO_Central.
DR   GO; GO:0009615; P:response to virus; TAS:ProtInc.
DR   GO; GO:0002286; P:T cell activation involved in immune response; IBA:GO_Central.
DR   GO; GO:0060337; P:type I interferon-mediated signaling pathway; NAS:ComplexPortal.
DR   CDD; cd00095; IFab; 1.
DR   Gene3D; 1.20.1250.10; -; 1.
DR   InterPro; IPR009079; 4_helix_cytokine-like_core.
DR   InterPro; IPR000471; Interferon_alpha/beta/delta.
DR   PANTHER; PTHR11691:SF37; INTERFERON OMEGA-1; 1.
DR   PANTHER; PTHR11691; TYPE I INTERFERON; 1.
DR   Pfam; PF00143; Interferon; 1.
DR   PRINTS; PR00266; INTERFERONAB.
DR   SMART; SM00076; IFabd; 1.
DR   SUPFAM; SSF47266; 4-helical cytokines; 1.
DR   PROSITE; PS00252; INTERFERON_A_B_D; 1.
DR   Genevisible; P05000; HS.
PE   1: Evidence at protein level;
KW   3D-structure; Antiviral defense; Cytokine; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /note="Or 23 in some molecules"
FT                   /evidence="ECO:0000269|PubMed:15340161,
FT                   ECO:0000269|PubMed:1693148, ECO:0000269|PubMed:7765487"
FT   CHAIN           22..195
FT                   /note="Interferon omega-1"
FT                   /id="PRO_0000016408"
FT   CARBOHYD        101
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:1693148"
FT                   /id="CAR_000050"
FT   DISULFID        24..122
FT                   /evidence="ECO:0000250"
FT   DISULFID        52..162
FT                   /evidence="ECO:0000269|PubMed:21854986"
FT   VARIANT         95
FT                   /note="R -> S (in dbSNP:rs2230055)"
FT                   /id="VAR_020028"
FT   CONFLICT        22..23
FT                   /note="LG -> EF (in Ref. 7; AAA70091)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        42
FT                   /note="H -> M (in Ref. 8; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        79
FT                   /note="V -> A (in Ref. 7; AAA70091)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        111
FT                   /note="G -> E (in Ref. 1; AAA52724)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        167
FT                   /note="V -> D (in Ref. 7; AAA70091)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   195 AA;  22319 MW;  1E4306F3487987FA CRC64;
     MALLFPLLAA LVMTSYSPVG SLGCDLPQNH GLLSRNTLVL LHQMRRISPF LCLKDRRDFR
     FPQEMVKGSQ LQKAHVMSVL HEMLQQIFSL FHTERSSAAW NMTLLDQLHT GLHQQLQHLE
     TCLLQVVGEG ESAGAISSPA LTLRRYFQGI RVYLKEKKYS DCAWEVVRME IMKSLFLSTN
     MQERLRSKDR DLGSS
//