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Protein

Exodeoxyribonuclease 7 large subunit

Gene

xseA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides. It can also degrade 3' or 5' ss regions extending from the termini of duplex DNA molecules and displaced ss regions.

Catalytic activityi

Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-direction to yield nucleoside 5'-phosphates.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BioCyciEcoCyc:EG11072-MONOMER.
ECOL316407:JW2493-MONOMER.
MetaCyc:EG11072-MONOMER.
BRENDAi3.1.11.6. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Exodeoxyribonuclease 7 large subunitUniRule annotation (EC:3.1.11.6UniRule annotation)
Alternative name(s):
Exodeoxyribonuclease VII large subunitUniRule annotation
Short name:
Exonuclease VII large subunitUniRule annotation
Gene namesi
Name:xseAUniRule annotation
Ordered Locus Names:b2509, JW2493
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11072. xseA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Exodeoxyribonuclease 7 large subunitPRO_0000197845Add
BLAST

Proteomic databases

PaxDbiP04994.
PRIDEiP04994.

Interactioni

Subunit structurei

Heterooligomer composed of two different subunits with an approximate ratio of 4 small subunits for 1 large.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
intQP761682EBI-559703,EBI-559711

Protein-protein interaction databases

BioGridi4263222. 57 interactions.
DIPiDIP-11146N.
IntActiP04994. 9 interactions.
MINTiMINT-1272208.
STRINGi511145.b2509.

Structurei

3D structure databases

ProteinModelPortaliP04994.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the XseA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DVS. Bacteria.
COG1570. LUCA.
HOGENOMiHOG000229265.
InParanoidiP04994.
KOiK03601.
OMAiENAQVRC.
PhylomeDBiP04994.

Family and domain databases

HAMAPiMF_00378. Exonuc_7_L. 1 hit.
InterProiIPR003753. Exonuc_VII_L.
IPR020579. Exonuc_VII_lsu_C.
IPR025824. OB-fold_nuc-bd_dom.
[Graphical view]
PfamiPF02601. Exonuc_VII_L. 1 hit.
PF13742. tRNA_anti_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00237. xseA. 1 hit.

Sequencei

Sequence statusi: Complete.

P04994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPSQSPAIF TVSRLNQTVR LLLEHEMGQV WISGEISNFT QPASGHWYFT
60 70 80 90 100
LKDDTAQVRC AMFRNSNRRV TFRPQHGQQV LVRANITLYE PRGDYQIIVE
110 120 130 140 150
SMQPAGEGLL QQKYEQLKAK LQAEGLFDQQ YKKPLPSPAH CVGVITSKTG
160 170 180 190 200
AALHDILHVL KRRDPSLPVI IYPAAVQGDD APGQIVRAIE LANQRNECDV
210 220 230 240 250
LIVGRGGGSL EDLWSFNDER VARAIFTSRI PVVSAVGHET DVTIADFVAD
260 270 280 290 300
LRAPTPSAAA EVVSRNQQEL LRQVQSTRQR LEMAMDYYLA NRTRRFTQIH
310 320 330 340 350
HRLQQQHPQL RLARQQTMLE RLQKRMSFAL ENQLKRTGQQ QQRLTQRLNQ
360 370 380 390 400
QNPQPKIHRA QTRIQQLEYR LAETLRAQLS ATRERFGNAV THLEAVSPLS
410 420 430 440 450
TLARGYSVTT ATDGNVLKKV KQVKAGEMLT TRLEDGWIES EVKNIQPVKK

SRKKVH
Length:456
Mass (Da):51,832
Last modified:November 1, 1997 - v2
Checksum:iA5E5B138C54A7D27
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti67 – 671N → S in AAA24766 (PubMed:3021756).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02599 Genomic DNA. Translation: AAA24766.1.
U00096 Genomic DNA. Translation: AAC75562.1.
AP009048 Genomic DNA. Translation: BAA16396.1.
PIRiD65027. NCEC7.
RefSeqiNP_417004.1. NC_000913.3.
WP_000937912.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75562; AAC75562; b2509.
BAA16396; BAA16396; BAA16396.
GeneIDi946988.
KEGGiecj:JW2493.
eco:b2509.
PATRICi32120411. VBIEscCol129921_2608.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02599 Genomic DNA. Translation: AAA24766.1.
U00096 Genomic DNA. Translation: AAC75562.1.
AP009048 Genomic DNA. Translation: BAA16396.1.
PIRiD65027. NCEC7.
RefSeqiNP_417004.1. NC_000913.3.
WP_000937912.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP04994.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263222. 57 interactions.
DIPiDIP-11146N.
IntActiP04994. 9 interactions.
MINTiMINT-1272208.
STRINGi511145.b2509.

Proteomic databases

PaxDbiP04994.
PRIDEiP04994.

Protocols and materials databases

DNASUi946988.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75562; AAC75562; b2509.
BAA16396; BAA16396; BAA16396.
GeneIDi946988.
KEGGiecj:JW2493.
eco:b2509.
PATRICi32120411. VBIEscCol129921_2608.

Organism-specific databases

EchoBASEiEB1065.
EcoGeneiEG11072. xseA.

Phylogenomic databases

eggNOGiENOG4105DVS. Bacteria.
COG1570. LUCA.
HOGENOMiHOG000229265.
InParanoidiP04994.
KOiK03601.
OMAiENAQVRC.
PhylomeDBiP04994.

Enzyme and pathway databases

BioCyciEcoCyc:EG11072-MONOMER.
ECOL316407:JW2493-MONOMER.
MetaCyc:EG11072-MONOMER.
BRENDAi3.1.11.6. 2026.

Miscellaneous databases

PROiP04994.

Family and domain databases

HAMAPiMF_00378. Exonuc_7_L. 1 hit.
InterProiIPR003753. Exonuc_VII_L.
IPR020579. Exonuc_VII_lsu_C.
IPR025824. OB-fold_nuc-bd_dom.
[Graphical view]
PfamiPF02601. Exonuc_VII_L. 1 hit.
PF13742. tRNA_anti_2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00237. xseA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEX7L_ECOLI
AccessioniPrimary (citable) accession number: P04994
Secondary accession number(s): P78230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.