Skip Header

Contribute Send feedback
Read comments (?) or add your own

P04993 (EX5A_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Exodeoxyribonuclease V alpha chain
EC=3.1.11.5
Alternative name(s):
Exodeoxyribonuclease V 67 kDa polypeptide
Gene names
Name:recD
Ordered Locus Names:b2819, JW2787
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length608 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exhibits several catalytic activities, including ATP-dependent exonuclease, ATP-stimulated endonuclease, ATP-dependent unwinding and DNA-dependent ATPase activities. Strand cleavage occurs 5' to 3' during the unwinding of duplex DNA at CHI sequences, which locally stimulate recombination.

Catalytic activity

Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.

Subunit structure

Consist of three subunits; recB, recC and recD.

Sequence similarities

Belongs to the recD family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 608608Exodeoxyribonuclease V alpha chain
PRO_0000087115

Regions

Nucleotide binding171 – 1788ATP Probable

Experimental info

Sequence conflict247 – 2482QP → HR in CAA28253. Ref.1
Sequence conflict3061A → V in CAA28253. Ref.1
Sequence conflict3901F → L in CAA28253. Ref.1

Secondary structure

1 .................................................................................................. 608
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04993 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: C37C62E17D41AA82

FASTA60866,902
        10         20         30         40         50         60 
MKLQKQLLEA VEHKQLRPLD VQFALTVAGD EHPAVTLAAA LLSHDAGEGH VCLPLSRLEN 

        70         80         90        100        110        120 
NEASHPLLAT CVSEIGELQN WEECLLASQA VSRGDEPTPM ILCGDRLYLN RMWCNERTVA 

       130        140        150        160        170        180 
RFFNEVNHAI EVDEALLAQT LDKLFPVSDE INWQKVAAAV ALTRRISVIS GGPGTGKTTT 

       190        200        210        220        230        240 
VAKLLAALIQ MADGERCRIR LAAPTGKAAA RLTESLGKAL RQLPLTDEQK KRIPEDASTL 

       250        260        270        280        290        300 
HRLLGAQPGS QRLRHHAGNP LHLDVLVVDE ASMIDLPMMS RLIDALPDHA RVIFLGDRDQ 

       310        320        330        340        350        360 
LASVEAGAVL GDICAYANAG FTAERARQLS RLTGTHVPAG TGTEAASLRD SLCLLQKSYR 

       370        380        390        400        410        420 
FGSDSGIGQL AAAINRGDKT AVKTVFQQDF TDIEKRLLQS GEDYIAMLEE ALAGYGRYLD 

       430        440        450        460        470        480 
LLQARAEPDL IIQAFNEYQL LCALREGPFG VAGLNERIEQ FMQQKRKIHR HPHSRWYEGR 

       490        500        510        520        530        540 
PVMIARNDSA LGLFNGDIGI ALDRGQGTRV WFAMPDGNIK SVQPSRLPEH ETTWAMTVHK 

       550        560        570        580        590        600 
SQGSEFDHAA LILPSQRTPV VTRELVYTAV TRARRRLSLY ADERILSAAI ATRTERRSGL 


AALFSSRE

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of recD, the structural gene for the alpha subunit of Exonuclease V of Escherichia coli."
Finch P.W., Storey A., Brown K., Hickson I.D., Emmerson P.T.
Nucleic Acids Res. 14:8583-8594(1986) [PubMed: 3537961] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Complete nucleotide sequence of the Escherichia coli recB gene."
Finch P.W., Storey A., Chapman K.E., Brown K., Hickson I.D., Emmerson P.T.
Nucleic Acids Res. 14:8573-8582(1986) [PubMed: 3537960] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-31.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X04582 Genomic DNA. Translation: CAA28253.1.
U29581 Genomic DNA. Translation: AAB40466.1.
U00096 Genomic DNA. Translation: AAC75858.1.
AP009048 Genomic DNA. Translation: BAE76888.1.
X04581 Genomic DNA. Translation: CAA28251.1.
PIRNCECXF. D65064.
RefSeqNP_417296.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1W36X-ray3.10D/G1-608[»]
3K70X-ray3.59D/G1-608[»]
ProteinModelPortalP04993.
SMRP04993. Positions 1-466, 523-606.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10651N.
IntActP04993. 11 interactions.
MINTMINT-1224412.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002176; EBESCP00000002176; EBESCG00000001783.
EBESCT00000016627; EBESCP00000015918; EBESCG00000015686.
GeneID947287.
GenomeReviewsGene locus JW2787 in contig AP009048_GR.
Gene locus b2819 in contig U00096_GR.
KEGGecj:JW2787.
eco:b2819.
PATRIC32121056. VBIEscCol129921_2917.

Organism-specific databases

EchoBASEEB0819.
EcoGeneEG10826. recD.

Phylogenomic databases

eggNOGCOG0507.
GeneTreeEBGT00050000009634.
HOGENOMHBG482408.
OMARQTDEFA.
PhylomeDBP04993.
ProtClustDBPRK10875.

Enzyme and pathway databases

BioCycEcoCyc:EG10826-MONOMER.
MetaCyc:EG10826-MONOMER.

Gene expression databases

GenevestigatorP04993.

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR007807. DUF699_exoDNase-V_alpha.
IPR006344. ExoDNase_V_RecD_asu.
IPR000606. Helicase_domain_viral-like.
[Graphical view]
KOK03581.
PfamPF05127. DUF699. 1 hit.
PF01443. Viral_helicase1. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
TIGRFAMsTIGR01447. RecD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameEX5A_ECOLI
AccessionPrimary (citable) accession number: P04993
Secondary accession number(s): Q2MA18, Q59378
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1997
Last modified: January 25, 2012
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents