P04988 (CYSP1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine proteinase 1
EC=3.4.22.-

Gene names

Name:	cprA
Synonyms:	CP1
ORF Names:	DDB_G0290957

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cysteine proteinases 1 and 2 are believed to participate in the breakdown of protein during differentiation of Dictyostelium as a response to starvation.
Subcellular location	Lysosome.
Post-translational modification	Phosphoglycosylated, contains GlcNAc-alpha-1-P-Ser residues. Ref.3
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Phosphoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hyperosmotic response Inferred from expression pattern PubMed 17517120. Source: dictyBase proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 117	99	Activation peptide		PRO_0000026358
	Chain	118 – 343	226	Cysteine proteinase 1		PRO_0000026359
Sites
	Active site	142	1	By similarity
	Active site	286	1	By similarity
	Active site	311	1	By similarity
Amino acid modifications
	Disulfide bond	139 ↔ 190		By similarity
	Disulfide bond	173 ↔ 224		By similarity
	Disulfide bond	279 ↔ 332		By similarity
Experimental info
	Sequence conflict	23	1	L → P in CAA26255. Ref.1
	Sequence conflict	184	1	Q → E in CAA26255. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P04988 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 8F70D4907C891021	FASTA	343	38,510
	10 20 30 40 50 60 MKVILLFVLA VFTVFVSSRG IPLEEQSQFL EFQDKFNKKY SHEEYLERFE IFKSNLGKIE 70 80 90 100 110 120 ELNLIAINHK ADTKFGVNKF ADLSSDEFKN YYLNNKEAIF TDDLPVADYL DDEFINSIPT 130 140 150 160 170 180 AFDWRTRGAV TPVKNQGQCG SCWSFSTTGN VEGQHFISQN KLVSLSEQNL VDCDHECMEY 190 200 210 220 230 240 EGEQACDEGC NGGLQPNAYN YIIKNGGIQT ESSYPYTAET GTQCNFNSAN IGAKISNFTM 250 260 270 280 290 300 IPKNETVMAG YIVSTGPLAI AADAVEWQFY IGGVFDIPCN PNSLDHGILI VGYSAKNTIF 310 320 330 340 RKNMPYWIVK NSWGADWGEQ GYIYLRRGKN TCGVSNFVST SII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A developmentally regulated cysteine proteinase in Dictyostelium discoideum." Williams J.G., North M.J., Mahbubani H.M. EMBO J. 4:999-1006(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"A lysosomal cysteine proteinase from Dictyostelium discoideum contains N-acetylglucosamine-1-phosphate bound to serine but not mannose-6-phosphate on N-linked oligosaccharides." Mehta D.P., Ichikawa M., Salimath P.V., Etchison J.R., Haak R., Manzi A., Freeze H.H. J. Biol. Chem. 271:10897-10903(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02407 mRNA. Translation: CAA26255.1. AAFI02000174 Genomic DNA. Translation: EAL61909.1.
PIR	KHDO. A22827.
RefSeq	XP_635417.1. XM_630325.1.
3D structure databases
ProteinModelPortal	P04988.
ModBase	Search...
Protein family/group databases
MEROPS	C01.022.
PTM databases
GlycoSuiteDB	P04988.
2D gel databases
SWISS-2DPAGE	P04988.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0201647; DDB0201647; DDB_G0290957.
GeneID	8627918.
KEGG	ddi:DDB_G0290957.
Organism-specific databases
dictyBase	DDB_G0290957. cprA.
Phylogenomic databases
eggNOG	COG4870.
KO	K01376.
OMA	KISNFTM.
ProtClustDB	CLSZ2429603.
Family and domain databases
InterPro	IPR025661. Pept_asp_AS. IPR000169. Pept_cys_AS. IPR025660. Pept_his_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. IPR013201. Prot_inhib_I29. [Graphical view]
PANTHER	PTHR12411. PTHR12411. 1 hit.
Pfam	PF08246. Inhibitor_I29. 1 hit. PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00848. Inhibitor_I29. 1 hit. SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSP1_DICDI

Accession

Primary (citable) accession number: P04988
Secondary accession number(s): Q54FC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	December 4, 2007
Last modified:	May 1, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)