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P04988

- CYSP1_DICDI

UniProt

P04988 - CYSP1_DICDI

Protein

Cysteine proteinase 1

Gene

cprA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Cysteine proteinases 1 and 2 are believed to participate in the breakdown of protein during differentiation of Dictyostelium as a response to starvation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei142 – 1421By similarity
    Active sitei286 – 2861By similarity
    Active sitei311 – 3111By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. hyperosmotic response Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Enzyme and pathway databases

    ReactomeiREACT_204779. MHC class II antigen presentation.

    Protein family/group databases

    MEROPSiC01.022.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cysteine proteinase 1 (EC:3.4.22.-)
    Gene namesi
    Name:cprA
    Synonyms:CP1
    ORF Names:DDB_G0290957
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0290957. cprA.

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 11799Activation peptidePRO_0000026358Add
    BLAST
    Chaini118 – 343226Cysteine proteinase 1PRO_0000026359Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi139 ↔ 190By similarity
    Disulfide bondi173 ↔ 224By similarity
    Disulfide bondi279 ↔ 332By similarity

    Post-translational modificationi

    Phosphoglycosylated, contains GlcNAc-alpha-1-P-Ser residues.

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

    2D gel databases

    SWISS-2DPAGEP04988.

    PTM databases

    UniCarbKBiP04988.

    Structurei

    3D structure databases

    ProteinModelPortaliP04988.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4870.
    KOiK01373.
    OMAiYSHEEYL.
    PhylomeDBiP04988.

    Family and domain databases

    InterProiIPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view]
    PANTHERiPTHR12411. PTHR12411. 1 hit.
    PfamiPF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view]
    PRINTSiPR00705. PAPAIN.
    SMARTiSM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view]
    PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04988-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVILLFVLA VFTVFVSSRG IPLEEQSQFL EFQDKFNKKY SHEEYLERFE    50
    IFKSNLGKIE ELNLIAINHK ADTKFGVNKF ADLSSDEFKN YYLNNKEAIF 100
    TDDLPVADYL DDEFINSIPT AFDWRTRGAV TPVKNQGQCG SCWSFSTTGN 150
    VEGQHFISQN KLVSLSEQNL VDCDHECMEY EGEQACDEGC NGGLQPNAYN 200
    YIIKNGGIQT ESSYPYTAET GTQCNFNSAN IGAKISNFTM IPKNETVMAG 250
    YIVSTGPLAI AADAVEWQFY IGGVFDIPCN PNSLDHGILI VGYSAKNTIF 300
    RKNMPYWIVK NSWGADWGEQ GYIYLRRGKN TCGVSNFVST SII 343
    Length:343
    Mass (Da):38,510
    Last modified:December 4, 2007 - v2
    Checksum:i8F70D4907C891021
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231L → P in CAA26255. (PubMed:2990918)Curated
    Sequence conflicti184 – 1841Q → E in CAA26255. (PubMed:2990918)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02407 mRNA. Translation: CAA26255.1.
    AAFI02000174 Genomic DNA. Translation: EAL61909.1.
    PIRiA22827. KHDO.
    RefSeqiXP_635417.1. XM_630325.1.

    Genome annotation databases

    EnsemblProtistsiDDB0201647; DDB0201647; DDB_G0290957.
    GeneIDi8627918.
    KEGGiddi:DDB_G0290957.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02407 mRNA. Translation: CAA26255.1 .
    AAFI02000174 Genomic DNA. Translation: EAL61909.1 .
    PIRi A22827. KHDO.
    RefSeqi XP_635417.1. XM_630325.1.

    3D structure databases

    ProteinModelPortali P04988.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C01.022.

    PTM databases

    UniCarbKBi P04988.

    2D gel databases

    SWISS-2DPAGE P04988.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0201647 ; DDB0201647 ; DDB_G0290957 .
    GeneIDi 8627918.
    KEGGi ddi:DDB_G0290957.

    Organism-specific databases

    dictyBasei DDB_G0290957. cprA.

    Phylogenomic databases

    eggNOGi COG4870.
    KOi K01373.
    OMAi YSHEEYL.
    PhylomeDBi P04988.

    Enzyme and pathway databases

    Reactomei REACT_204779. MHC class II antigen presentation.

    Family and domain databases

    InterProi IPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view ]
    PANTHERi PTHR12411. PTHR12411. 1 hit.
    Pfami PF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view ]
    PRINTSi PR00705. PAPAIN.
    SMARTi SM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view ]
    PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A developmentally regulated cysteine proteinase in Dictyostelium discoideum."
      Williams J.G., North M.J., Mahbubani H.M.
      EMBO J. 4:999-1006(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "A lysosomal cysteine proteinase from Dictyostelium discoideum contains N-acetylglucosamine-1-phosphate bound to serine but not mannose-6-phosphate on N-linked oligosaccharides."
      Mehta D.P., Ichikawa M., Salimath P.V., Etchison J.R., Haak R., Manzi A., Freeze H.H.
      J. Biol. Chem. 271:10897-10903(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE OF CARBOHYDRATES.

    Entry informationi

    Entry nameiCYSP1_DICDI
    AccessioniPrimary (citable) accession number: P04988
    Secondary accession number(s): Q54FC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3