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P04988

- CYSP1_DICDI

UniProt

P04988 - CYSP1_DICDI

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Protein

Cysteine proteinase 1

Gene
cprA, CP1, DDB_G0290957
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Cysteine proteinases 1 and 2 are believed to participate in the breakdown of protein during differentiation of Dictyostelium as a response to starvation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei142 – 1421 By similarity
Active sitei286 – 2861 By similarity
Active sitei311 – 3111 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. hyperosmotic response Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

ReactomeiREACT_204779. MHC class II antigen presentation.

Protein family/group databases

MEROPSiC01.022.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine proteinase 1 (EC:3.4.22.-)
Gene namesi
Name:cprA
Synonyms:CP1
ORF Names:DDB_G0290957
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290957. cprA.

Subcellular locationi

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818 Reviewed predictionAdd
BLAST
Propeptidei19 – 11799Activation peptidePRO_0000026358Add
BLAST
Chaini118 – 343226Cysteine proteinase 1PRO_0000026359Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 190 By similarity
Disulfide bondi173 ↔ 224 By similarity
Disulfide bondi279 ↔ 332 By similarity

Post-translational modificationi

Phosphoglycosylated, contains GlcNAc-alpha-1-P-Ser residues.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Zymogen

2D gel databases

SWISS-2DPAGEP04988.

PTM databases

UniCarbKBiP04988.

Structurei

3D structure databases

ProteinModelPortaliP04988.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4870.
KOiK01373.
OMAiYSHEEYL.
PhylomeDBiP04988.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04988-1 [UniParc]FASTAAdd to Basket

« Hide

MKVILLFVLA VFTVFVSSRG IPLEEQSQFL EFQDKFNKKY SHEEYLERFE    50
IFKSNLGKIE ELNLIAINHK ADTKFGVNKF ADLSSDEFKN YYLNNKEAIF 100
TDDLPVADYL DDEFINSIPT AFDWRTRGAV TPVKNQGQCG SCWSFSTTGN 150
VEGQHFISQN KLVSLSEQNL VDCDHECMEY EGEQACDEGC NGGLQPNAYN 200
YIIKNGGIQT ESSYPYTAET GTQCNFNSAN IGAKISNFTM IPKNETVMAG 250
YIVSTGPLAI AADAVEWQFY IGGVFDIPCN PNSLDHGILI VGYSAKNTIF 300
RKNMPYWIVK NSWGADWGEQ GYIYLRRGKN TCGVSNFVST SII 343
Length:343
Mass (Da):38,510
Last modified:December 4, 2007 - v2
Checksum:i8F70D4907C891021
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231L → P in CAA26255. 1 Publication
Sequence conflicti184 – 1841Q → E in CAA26255. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02407 mRNA. Translation: CAA26255.1.
AAFI02000174 Genomic DNA. Translation: EAL61909.1.
PIRiA22827. KHDO.
RefSeqiXP_635417.1. XM_630325.1.

Genome annotation databases

EnsemblProtistsiDDB0201647; DDB0201647; DDB_G0290957.
GeneIDi8627918.
KEGGiddi:DDB_G0290957.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X02407 mRNA. Translation: CAA26255.1 .
AAFI02000174 Genomic DNA. Translation: EAL61909.1 .
PIRi A22827. KHDO.
RefSeqi XP_635417.1. XM_630325.1.

3D structure databases

ProteinModelPortali P04988.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C01.022.

PTM databases

UniCarbKBi P04988.

2D gel databases

SWISS-2DPAGE P04988.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0201647 ; DDB0201647 ; DDB_G0290957 .
GeneIDi 8627918.
KEGGi ddi:DDB_G0290957.

Organism-specific databases

dictyBasei DDB_G0290957. cprA.

Phylogenomic databases

eggNOGi COG4870.
KOi K01373.
OMAi YSHEEYL.
PhylomeDBi P04988.

Enzyme and pathway databases

Reactomei REACT_204779. MHC class II antigen presentation.

Family and domain databases

InterProi IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view ]
PANTHERi PTHR12411. PTHR12411. 1 hit.
Pfami PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view ]
PRINTSi PR00705. PAPAIN.
SMARTi SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view ]
PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A developmentally regulated cysteine proteinase in Dictyostelium discoideum."
    Williams J.G., North M.J., Mahbubani H.M.
    EMBO J. 4:999-1006(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  3. "A lysosomal cysteine proteinase from Dictyostelium discoideum contains N-acetylglucosamine-1-phosphate bound to serine but not mannose-6-phosphate on N-linked oligosaccharides."
    Mehta D.P., Ichikawa M., Salimath P.V., Etchison J.R., Haak R., Manzi A., Freeze H.H.
    J. Biol. Chem. 271:10897-10903(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE OF CARBOHYDRATES.

Entry informationi

Entry nameiCYSP1_DICDI
AccessioniPrimary (citable) accession number: P04988
Secondary accession number(s): Q54FC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 4, 2007
Last modified: September 3, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi