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Protein

Elastin

Gene

ELN

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Elastin
Alternative name(s):
Tropoelastin
Gene namesi
Name:ELN
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Secretedextracellular spaceextracellular matrix By similarity

  • Note: Extracellular matrix of elastic fibers.By similarity

GO - Cellular componenti

  • elastic fiber Source: AgBase
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26By similarityAdd BLAST26
ChainiPRO_000002116127 – 747ElastinAdd BLAST721

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei344-hydroxyprolineBy similarity1
Modified residuei654-hydroxyprolineBy similarity1
Modified residuei874-hydroxyprolineBy similarity1
Modified residuei105Allysine1
Modified residuei109Allysine1
Modified residuei1654-hydroxyprolineBy similarity1
Modified residuei1784-hydroxyprolineBy similarity1
Modified residuei1814-hydroxyprolineBy similarity1
Modified residuei188HydroxyprolineBy similarity1
Modified residuei2014-hydroxyprolineBy similarity1
Modified residuei252Allysine1
Modified residuei271Allysine1
Modified residuei275Allysine1
Modified residuei2984-hydroxyprolineBy similarity1
Modified residuei3024-hydroxyprolineBy similarity1
Modified residuei324Allysine1
Modified residuei327Allysine1
Modified residuei3354-hydroxyprolineBy similarity1
Modified residuei3654-hydroxyprolineBy similarity1
Modified residuei3704-hydroxyprolineBy similarity1
Modified residuei3754-hydroxyprolineBy similarity1
Modified residuei3804-hydroxyprolineBy similarity1
Modified residuei3854-hydroxyprolineBy similarity1
Modified residuei400Allysine1
Modified residuei404Allysine1
Modified residuei407Allysine1
Modified residuei448Allysine1
Modified residuei4624-hydroxyprolineBy similarity1
Modified residuei4784-hydroxyprolineBy similarity1
Modified residuei489Allysine1
Modified residuei493Allysine1
Modified residuei5134-hydroxyprolineBy similarity1
Modified residuei544Allysine1
Modified residuei548Allysine1
Modified residuei552Allysine1
Modified residuei5664-hydroxyprolineBy similarity1
Modified residuei5754-hydroxyprolineBy similarity1
Modified residuei5844-hydroxyprolineBy similarity1
Modified residuei5934-hydroxyprolineBy similarity1
Modified residuei5994-hydroxyprolineBy similarity1
Modified residuei606Allysine1
Modified residuei609Allysine1
Modified residuei6304-hydroxyprolineBy similarity1
Modified residuei645Allysine1
Modified residuei649Allysine1
Modified residuei685Allysine1
Modified residuei688Allysine1
Modified residuei7194-hydroxyprolineBy similarity1
Modified residuei7334-hydroxyprolineBy similarity1
Disulfide bondi737 ↔ 7421 Publication

Post-translational modificationi

Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.By similarity

Keywords - PTMi

Disulfide bond, Hydroxylation

Proteomic databases

PRIDEiP04985.

Interactioni

Subunit structurei

The polymeric elastin chains are cross-linked together into an extensible 3D network. Forms a ternary complex with BGN and MFAP2. Interacts with MFAP2 via divalent cations (calcium > magnesium > manganese) in a dose-dependent and saturating manner (PubMed:11723132). Interacts with FBLN5 and FBN1. Forms a ternary complex with FBN1 and FBLN2 or FBLN5 (By similarity). Interacts with MFAP4 in a Ca (2+)-dependent manner; this interaction promotes ELN self-assembly (PubMed:26601954).By similarity2 Publications

Protein-protein interaction databases

DIPiDIP-35453N.
IntActiP04985. 3 interactors.

Family & Domainsi

Sequence similaritiesi

Belongs to the elastin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

InParanoidiP04985.

Family and domain databases

InterProiIPR003979. Tropoelastin.
[Graphical view]
PRINTSiPR01500. TROPOELASTIN.

Sequences (7)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 7 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04985-1) [UniParc]FASTAAdd to basket
Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRSLTAAARR PEVLLLLLCI LQPSQPGGVP GAVPGGVPGG VFFPGAGLGG
60 70 80 90 100
LGVGGLGPGV KPAKPGVGGL VGPGLGAEGS ALPGAFPGGF FGAGGGAAGA
110 120 130 140 150
AAAYKAAAKA GAAGLGVGGI GGVGGLGVST GAVVPQLGAG VGAGVKPGKV
160 170 180 190 200
PGVGLPGVYP GGVLPGAGAR FPGIGVLPGV PTGAGVKPKA QVGAGAFAGI
210 220 230 240 250
PGVGPFGGQQ PGLPLGYPIK APKLPAGYGL PYKTGKLPYG FGPGGVAGSA
260 270 280 290 300
GKAGYPTGTG VGPQAAAAAA KAAAKLGAGG AGVLPGVGVG GPGIPGAPGA
310 320 330 340 350
IPGIGGIAGV GAPDAAAAAA AAAKAAKFGA AGGLPGVGVP GVGVPGVGVP
360 370 380 390 400
GVGVPGVGVP GVGVPGVGVP GVGVPGVGVP GVGVPGVGVP GALSPAATAK
410 420 430 440 450
AAAKAAKFGA RGAVGIGGIP TFGLGPGGFP GIGDAAAAPA AAAAKAAKIG
460 470 480 490 500
AGGVGALGGV VPGAPGAIPG LPGVGGVPGV GIPAAAAAKA AAKAAQFGLG
510 520 530 540 550
PGVGVAPGVG VVPGVGVVPG VGVAPGIGLG PGGVIGAGVP AAAKSAAKAA
560 570 580 590 600
AKAQFRAAAG LPAGVPGLGV GAGVPGLGVG AGVPGLGVGA GVPGPGAVPG
610 620 630 640 650
TLAAAKAAKF GPGGVGALGG VGDLGGAGIP GGVAGVVPAA AAAAKAAAKA
660 670 680 690 700
AQFGLGGVGG LGVGGLGAVP GAVGLGGVSP AAAAKAAKFG AAGLGGVLGA
710 720 730 740
GQPFPIGGGA GGLGVGGKPP KPFGGALGAL GFPGGACLGK SCGRKRK
Length:747
Mass (Da):64,229
Last modified:August 13, 1987 - v1
Checksum:i633C03E411643D83
GO
Isoform 2 (identifier: P04985-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     226-239: Missing.

Show »
Length:733
Mass (Da):62,719
Checksum:i80632AC1522BE148
GO
Isoform 3 (identifier: P04985-3) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     226-259: Missing.

Show »
Length:713
Mass (Da):61,028
Checksum:i0544AAD9E999FC98
GO
Isoform 4 (identifier: P04985-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     708-708: G → GVAARPGFGLSPIFPG

Show »
Length:762
Mass (Da):65,697
Checksum:iD54BB492D60EDBEE
GO
Isoform 5 (identifier: P04985-5) [UniParc]FASTAAdd to basket
Also known as: Elastin-cBEL2

The sequence of this isoform differs from the canonical sequence as follows:
     598-610: Missing.

Show »
Length:734
Mass (Da):63,003
Checksum:i97CAD6B881E3AC43
GO
Isoform 6 (identifier: P04985-6) [UniParc]FASTAAdd to basket
Also known as: Elastin-cBEL3

The sequence of this isoform differs from the canonical sequence as follows:
     226-259: Missing.
     499-499: L → LALLAFAGL

Show »
Length:721
Mass (Da):61,785
Checksum:i15C6786E94469984
GO
Isoform 7 (identifier: P04985-7) [UniParc]FASTAAdd to basket
Also known as: Elastin-cBEL1

The sequence of this isoform differs from the canonical sequence as follows:
     226-259: Missing.
     654-676: Missing.
     708-708: G → GVAARPGFGLSPIFPG

Show »
Length:705
Mass (Da):60,723
Checksum:iD52D3EC4EFF2D5F3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 3MRS → MAG (PubMed:2543440).Curated3
Sequence conflicti1 – 3MRS → MAG (PubMed:2031719).Curated3
Sequence conflicti12E → G (PubMed:2543440).Curated1
Sequence conflicti12E → G (PubMed:2031719).Curated1
Sequence conflicti78 – 80EGS → GLG (PubMed:2992576).Curated3
Sequence conflicti78 – 80EGS → GLG (PubMed:3665402).Curated3
Sequence conflicti89 – 97GFFGAGGGA → ALVPGGP (PubMed:2992576).Curated9
Sequence conflicti89 – 97GFFGAGGGA → ALVPGGP (PubMed:3665402).Curated9
Sequence conflicti191 – 194QVGA → PGGG (PubMed:2992576).Curated4
Sequence conflicti191 – 194QVGA → PGGG (PubMed:3665402).Curated4
Sequence conflicti213L → V (PubMed:2992576).Curated1
Sequence conflicti213L → V (PubMed:3665402).Curated1
Sequence conflicti292P → A (PubMed:2992576).Curated1
Sequence conflicti292P → A (PubMed:3665402).Curated1
Sequence conflicti334L → F (PubMed:2992576).Curated1
Sequence conflicti334L → F (PubMed:3665402).Curated1
Sequence conflicti393L → V (PubMed:2992576).Curated1
Sequence conflicti393L → V (PubMed:3665402).Curated1
Sequence conflicti398T → A (PubMed:2992576).Curated1
Sequence conflicti398T → A (PubMed:3665402).Curated1
Sequence conflicti413A → G (PubMed:2992576).Curated1
Sequence conflicti413A → G (PubMed:3665402).Curated1
Sequence conflicti424L → V (PubMed:2992576).Curated1
Sequence conflicti424L → V (PubMed:3665402).Curated1
Sequence conflicti439P → Q (PubMed:2992576).Curated1
Sequence conflicti439P → Q (PubMed:3665402).Curated1
Sequence conflicti460V → L (PubMed:2992576).Curated1
Sequence conflicti460V → L (PubMed:3665402).Curated1
Sequence conflicti471L → V (PubMed:2992576).Curated1
Sequence conflicti471L → V (PubMed:3665402).Curated1
Sequence conflicti572A → V (PubMed:2992576).Curated1
Sequence conflicti572A → V (PubMed:3665402).Curated1
Sequence conflicti581A → V (PubMed:2992576).Curated1
Sequence conflicti581A → V (PubMed:3665402).Curated1
Sequence conflicti595P → F (PubMed:2992576).Curated1
Sequence conflicti595P → F (PubMed:3665402).Curated1
Sequence conflicti637V → G (PubMed:2992576).Curated1
Sequence conflicti637V → G (PubMed:3665402).Curated1
Sequence conflicti681Missing (PubMed:6150137).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004240226 – 259Missing in isoform 3, isoform 6 and isoform 7. CuratedAdd BLAST34
Alternative sequenceiVSP_004239226 – 239Missing in isoform 2. CuratedAdd BLAST14
Alternative sequenceiVSP_011940499L → LALLAFAGL in isoform 6. Curated1
Alternative sequenceiVSP_011941598 – 610Missing in isoform 5. CuratedAdd BLAST13
Alternative sequenceiVSP_011942654 – 676Missing in isoform 7. CuratedAdd BLAST23
Alternative sequenceiVSP_011943708G → GVAARPGFGLSPIFPG in isoform 4 and isoform 7. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02717 mRNA. Translation: AAA30503.1.
K03505 mRNA. Translation: AAA30505.1.
K03506 mRNA. Translation: AAA30506.1.
J02855 Genomic DNA. Translation: AAA30776.1.
M58652 Genomic DNA. Translation: AAA03519.2.
M19372
, M11422, M19366, M19367, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22775, M22988, M23010 Genomic DNA. Translation: AAA30498.1.
M19372
, M11422, M19366, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22775, M22988, M23010 Genomic DNA. Translation: AAA30499.1.
M19372
, M11422, M19366, M19367, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22988 Genomic DNA. Translation: AAA30500.1.
M19372
, M11422, M19366, M19367, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22775, M22988 Genomic DNA. Translation: AAA30501.1.
M31898
, M31894, M31895, M31896, M31897 Genomic DNA. Translation: AAA96417.1.
PIRiA31865. EABO.
UniGeneiBt.5361.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02717 mRNA. Translation: AAA30503.1.
K03505 mRNA. Translation: AAA30505.1.
K03506 mRNA. Translation: AAA30506.1.
J02855 Genomic DNA. Translation: AAA30776.1.
M58652 Genomic DNA. Translation: AAA03519.2.
M19372
, M11422, M19366, M19367, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22775, M22988, M23010 Genomic DNA. Translation: AAA30498.1.
M19372
, M11422, M19366, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22775, M22988, M23010 Genomic DNA. Translation: AAA30499.1.
M19372
, M11422, M19366, M19367, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22988 Genomic DNA. Translation: AAA30500.1.
M19372
, M11422, M19366, M19367, M19368, M19369, M19370, M19371, M22771, M22772, M22773, M22774, M22775, M22988 Genomic DNA. Translation: AAA30501.1.
M31898
, M31894, M31895, M31896, M31897 Genomic DNA. Translation: AAA96417.1.
PIRiA31865. EABO.
UniGeneiBt.5361.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-35453N.
IntActiP04985. 3 interactors.

Proteomic databases

PRIDEiP04985.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiP04985.

Family and domain databases

InterProiIPR003979. Tropoelastin.
[Graphical view]
PRINTSiPR01500. TROPOELASTIN.
ProtoNetiSearch...

Entry informationi

Entry nameiELN_BOVIN
AccessioniPrimary (citable) accession number: P04985
Secondary accession number(s): P04986
, P04987, Q28096, Q28097, Q28098, Q28099, Q28101, Q29421
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 5, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.