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Protein

Ribose import ATP-binding protein RbsA

Gene

rbsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex RbsABC involved in ribose import. Responsible for energy coupling to the transport system.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O + monosaccharide(Out) = ADP + phosphate + monosaccharide(In).UniRule annotation1 Publication

Kineticsi

  1. KM=140 µM for ATP1 Publication

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi37 – 44ATPUniRule annotation8

    GO - Molecular functioni

    • ATP binding Source: EcoCyc
    • D-ribose transmembrane transporter activity Source: EcoCyc
    • monosaccharide-transporting ATPase activity Source: EcoCyc

    GO - Biological processi

    • D-ribose transmembrane transport Source: EcoCyc

    Keywordsi

    Molecular functionHydrolase
    Biological processSugar transport, Transport
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:RBSA-MONOMER
    MetaCyc:RBSA-MONOMER

    Protein family/group databases

    TCDBi3.A.1.2.1 the atp-binding cassette (abc) superfamily

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribose import ATP-binding protein RbsAUniRule annotationCurated (EC:3.6.3.17UniRule annotation1 Publication)
    Gene namesi
    Name:rbsA1 PublicationUniRule annotation
    Ordered Locus Names:b3749, JW3728
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10814 rbsA

    Subcellular locationi

    GO - Cellular componenti

    • ATP-binding cassette (ABC) transporter complex Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi43K → R: Loss of transport. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000929561 – 501Ribose import ATP-binding protein RbsAAdd BLAST501

    Proteomic databases

    EPDiP04983
    PaxDbiP04983
    PRIDEiP04983

    Interactioni

    Subunit structurei

    The complex is composed of an ATP-binding protein (RbsA), two transmembrane proteins (RbsC) and a solute-binding protein (RbsB).UniRule annotation1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    phnGP166853EBI-1132449,EBI-9126715

    Protein-protein interaction databases

    BioGridi426345877 interactors.
    DIPiDIP-10640N
    IntActiP04983 11 interactors.
    STRINGi316385.ECDH10B_3937

    Structurei

    3D structure databases

    ProteinModelPortaliP04983
    SMRiP04983
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini5 – 241ABC transporter 1UniRule annotationAdd BLAST237
    Domaini252 – 495ABC transporter 2UniRule annotationAdd BLAST244

    Sequence similaritiesi

    Belongs to the ABC transporter superfamily. Ribose importer (TC 3.A.1.2.1) family. [View classification]UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiENOG4105C2J Bacteria
    COG1129 LUCA
    InParanoidiP04983
    KOiK10441
    OMAiIKMLSGA
    PhylomeDBiP04983

    Family and domain databases

    InterProiView protein in InterPro
    IPR003593 AAA+_ATPase
    IPR003439 ABC_transporter-like
    IPR017871 ABC_transporter_CS
    IPR015861 ABC_transpr_RbsA
    IPR027417 P-loop_NTPase
    PfamiView protein in Pfam
    PF00005 ABC_tran, 2 hits
    SMARTiView protein in SMART
    SM00382 AAA, 2 hits
    SUPFAMiSSF52540 SSF52540, 2 hits
    PROSITEiView protein in PROSITE
    PS00211 ABC_TRANSPORTER_1, 1 hit
    PS50893 ABC_TRANSPORTER_2, 1 hit
    PS51254 RBSA, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P04983-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEALLQLKGI DKAFPGVKAL SGAALNVYPG RVMALVGENG AGKSTMMKVL
    60 70 80 90 100
    TGIYTRDAGT LLWLGKETTF TGPKSSQEAG IGIIHQELNL IPQLTIAENI
    110 120 130 140 150
    FLGREFVNRF GKIDWKTMYA EADKLLAKLN LRFKSDKLVG DLSIGDQQMV
    160 170 180 190 200
    EIAKVLSFES KVIIMDEPTD ALTDTETESL FRVIRELKSQ GRGIVYISHR
    210 220 230 240 250
    MKEIFEICDD VTVFRDGQFI AEREVASLTE DSLIEMMVGR KLEDQYPHLD
    260 270 280 290 300
    KAPGDIRLKV DNLCGPGVND VSFTLRKGEI LGVSGLMGAG RTELMKVLYG
    310 320 330 340 350
    ALPRTSGYVT LDGHEVVTRS PQDGLANGIV YISEDRKRDG LVLGMSVKEN
    360 370 380 390 400
    MSLTALRYFS RAGGSLKHAD EQQAVSDFIR LFNVKTPSME QAIGLLSGGN
    410 420 430 440 450
    QQKVAIARGL MTRPKVLILD EPTRGVDVGA KKEIYQLINQ FKADGLSIIL
    460 470 480 490 500
    VSSEMPEVLG MSDRIIVMHE GHLSGEFTRE QATQEVLMAA AVGKLNRVNQ

    E
    Length:501
    Mass (Da):55,042
    Last modified:August 13, 1987 - v1
    Checksum:iA37B5F3A910A5D19
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M13169 Genomic DNA Translation: AAA51473.1
    L10328 Genomic DNA Translation: AAA62102.1
    U00096 Genomic DNA Translation: AAC76772.1
    AP009048 Genomic DNA Translation: BAE77539.1
    PIRiB26304
    RefSeqiNP_418205.1, NC_000913.3
    WP_000387770.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76772; AAC76772; b3749
    BAE77539; BAE77539; BAE77539
    GeneIDi948264
    KEGGiecj:JW3728
    eco:b3749
    PATRICifig|1411691.4.peg.2951

    Similar proteinsi

    Entry informationi

    Entry nameiRBSA_ECOLI
    AccessioniPrimary (citable) accession number: P04983
    Secondary accession number(s): Q2M867
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: April 25, 2018
    This is version 154 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome