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Protein

Ribose import ATP-binding protein RbsA

Gene

rbsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex RbsABC involved in ribose import. Responsible for energy coupling to the transport system.UniRule annotation3 Publications

Catalytic activityi

ATP + H2O + monosaccharide(Out) = ADP + phosphate + monosaccharide(In).UniRule annotation1 Publication

Kineticsi

  1. KM=140 µM for ATP1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi37 – 448ATPUniRule annotation

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sugar transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:RBSA-MONOMER.
    ECOL316407:JW3728-MONOMER.
    MetaCyc:RBSA-MONOMER.

    Protein family/group databases

    TCDBi3.A.1.2.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribose import ATP-binding protein RbsAUniRule annotationCurated (EC:3.6.3.17UniRule annotation1 Publication)
    Gene namesi
    Name:rbsA1 PublicationUniRule annotation
    Ordered Locus Names:b3749, JW3728
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10814. rbsA.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi43 – 431K → R: Loss of transport. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501Ribose import ATP-binding protein RbsAPRO_0000092956Add
    BLAST

    Proteomic databases

    EPDiP04983.
    PaxDbiP04983.
    PRIDEiP04983.

    Interactioni

    Subunit structurei

    The complex is composed of an ATP-binding protein (RbsA), two transmembrane proteins (RbsC) and a solute-binding protein (RbsB).UniRule annotation1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    phnGP166853EBI-1132449,EBI-9126715

    Protein-protein interaction databases

    BioGridi4263458. 73 interactions.
    DIPiDIP-10640N.
    IntActiP04983. 11 interactions.
    STRINGi511145.b3749.

    Structurei

    3D structure databases

    ProteinModelPortaliP04983.
    SMRiP04983. Positions 1-481.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 241237ABC transporter 1UniRule annotationAdd
    BLAST
    Domaini252 – 495244ABC transporter 2UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ABC transporter superfamily. Ribose importer (TC 3.A.1.2.1) family. [View classification]UniRule annotation
    Contains 2 ABC transporter domains.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiENOG4105C2J. Bacteria.
    COG1129. LUCA.
    InParanoidiP04983.
    KOiK10441.
    OMAiMDNMERI.
    PhylomeDBiP04983.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR015861. ABC_transpr_RbsA.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51254. RBSA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P04983-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEALLQLKGI DKAFPGVKAL SGAALNVYPG RVMALVGENG AGKSTMMKVL
    60 70 80 90 100
    TGIYTRDAGT LLWLGKETTF TGPKSSQEAG IGIIHQELNL IPQLTIAENI
    110 120 130 140 150
    FLGREFVNRF GKIDWKTMYA EADKLLAKLN LRFKSDKLVG DLSIGDQQMV
    160 170 180 190 200
    EIAKVLSFES KVIIMDEPTD ALTDTETESL FRVIRELKSQ GRGIVYISHR
    210 220 230 240 250
    MKEIFEICDD VTVFRDGQFI AEREVASLTE DSLIEMMVGR KLEDQYPHLD
    260 270 280 290 300
    KAPGDIRLKV DNLCGPGVND VSFTLRKGEI LGVSGLMGAG RTELMKVLYG
    310 320 330 340 350
    ALPRTSGYVT LDGHEVVTRS PQDGLANGIV YISEDRKRDG LVLGMSVKEN
    360 370 380 390 400
    MSLTALRYFS RAGGSLKHAD EQQAVSDFIR LFNVKTPSME QAIGLLSGGN
    410 420 430 440 450
    QQKVAIARGL MTRPKVLILD EPTRGVDVGA KKEIYQLINQ FKADGLSIIL
    460 470 480 490 500
    VSSEMPEVLG MSDRIIVMHE GHLSGEFTRE QATQEVLMAA AVGKLNRVNQ

    E
    Length:501
    Mass (Da):55,042
    Last modified:August 13, 1987 - v1
    Checksum:iA37B5F3A910A5D19
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M13169 Genomic DNA. Translation: AAA51473.1.
    L10328 Genomic DNA. Translation: AAA62102.1.
    U00096 Genomic DNA. Translation: AAC76772.1.
    AP009048 Genomic DNA. Translation: BAE77539.1.
    PIRiB26304.
    RefSeqiNP_418205.1. NC_000913.3.
    WP_000387770.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76772; AAC76772; b3749.
    BAE77539; BAE77539; BAE77539.
    GeneIDi948264.
    KEGGiecj:JW3728.
    eco:b3749.
    PATRICi32122997. VBIEscCol129921_3874.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M13169 Genomic DNA. Translation: AAA51473.1.
    L10328 Genomic DNA. Translation: AAA62102.1.
    U00096 Genomic DNA. Translation: AAC76772.1.
    AP009048 Genomic DNA. Translation: BAE77539.1.
    PIRiB26304.
    RefSeqiNP_418205.1. NC_000913.3.
    WP_000387770.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP04983.
    SMRiP04983. Positions 1-481.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4263458. 73 interactions.
    DIPiDIP-10640N.
    IntActiP04983. 11 interactions.
    STRINGi511145.b3749.

    Protein family/group databases

    TCDBi3.A.1.2.1. the atp-binding cassette (abc) superfamily.

    Proteomic databases

    EPDiP04983.
    PaxDbiP04983.
    PRIDEiP04983.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76772; AAC76772; b3749.
    BAE77539; BAE77539; BAE77539.
    GeneIDi948264.
    KEGGiecj:JW3728.
    eco:b3749.
    PATRICi32122997. VBIEscCol129921_3874.

    Organism-specific databases

    EchoBASEiEB0807.
    EcoGeneiEG10814. rbsA.

    Phylogenomic databases

    eggNOGiENOG4105C2J. Bacteria.
    COG1129. LUCA.
    InParanoidiP04983.
    KOiK10441.
    OMAiMDNMERI.
    PhylomeDBiP04983.

    Enzyme and pathway databases

    BioCyciEcoCyc:RBSA-MONOMER.
    ECOL316407:JW3728-MONOMER.
    MetaCyc:RBSA-MONOMER.

    Miscellaneous databases

    PROiP04983.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR015861. ABC_transpr_RbsA.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    PS51254. RBSA. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiRBSA_ECOLI
    AccessioniPrimary (citable) accession number: P04983
    Secondary accession number(s): Q2M867
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: September 7, 2016
    This is version 143 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.