Reviewed, UniProtKB/Swiss-Prot P04972 (CNRG_BOVIN)

Names and origin

Protein names

Recommended name:
    Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
      Short name=GMP-PDE gamma
    EC=3.1.4.17

Gene names

Name:	PDE6G
Synonyms:	PDEG

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Participates in processes of transmission and amplification of the visual signal. cGMP-PDEs are the effector molecules in G-protein-mediated phototransduction in vertebrate rods and cones.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Subunit structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Sequence similarities	Belongs to the rod/cone cGMP-PDE gamma subunit family.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Ligand	cGMP
Molecular function	Hydrolase
PTM	Acetylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3',5'-cyclic-nucleotide phosphodiesterase activity Inferred from electronic annotation. Source: EC cGMP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 87

87

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma

PRO_0000166113

Regions

Compositional bias

24 – 45

22

Arg/Lys-rich (basic)

Amino acid modifications

Modified residue

1

1

N-acetylmethionine

Secondary structure

1

.

.

.

.

.

.

.

87

Helix Strand Turn

Details...

Helix

62 – 65

4

Helix

69 – 73

5

Helix

78 – 83

6

Sequences

	Sequence		Length	Mass (Da)
	P04972-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 3AA2D6B5D7D14D58	FASTA	87	9,669
	10 20 30 40 50 60 MNLEPPKAEI RSATRVMGGP VTPRKGPPKF KQRQTRQFKS KPPKKGVQGF GDDIPGMEGL 70 80 GTDITVICPW EAFNHLELHE LAQYGII

References

[1]	"Cyclic GMP-phosphodiesterase from the cattle retina. Amino acid sequence of the gamma subunit." Ovchinnikov Y.A., Muradov K.G., Feigina M.Y., Nazimov I.V., Khoroshilova N.I. Dokl. Akad. Nauk SSSR 287:1496-1498(1986) [PubMed: 3011376] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Retina.
[2]	"Cyclic GMP phosphodiesterase from bovine retina. Nucleotide sequence of cDNA of the gamma-subunit." Ovchinnikov Y.A., Gubanov V.V., Khramtsov N.V., Akhmedov N.B., Zagranichny V.E., Ishchenko K.A., Muradov K.G., Barinov A.A., Bondarenko V.A., Kumarev V.P., Kobzev V.F., Lipkin V.M. Dokl. Biochem. 288:216-218(1986) Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[3]	"Cyclic GMP phosphodiesterase from cattle retina. Amino acid sequence of the gamma-subunit and nucleotide sequence of the corresponding cDNA." Ovchinnikov Y.A., Lipkin V.M., Kumarev V.P., Gubanov V.V., Khramtsov N.V., Akhmedov N.B., Zagranichny V.E., Muradov K.G. FEBS Lett. 204:288-292(1986) [PubMed: 3015681] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[4]	"Structural determinants for regulation of phosphodiesterase by a G protein at 2.0 A." Slep K.C., Kercher M.A., He W., Cowan C.W., Wensel T.G., Sigler P.B. Nature 409:1071-1077(2001) [PubMed: 11234020] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 46-87 IN COMPLEX WITH RGS9 AND GNAT1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X04823 mRNA. Translation: CAA28507.1.
X04270 mRNA. Translation: CAA27821.1.

IPI

IPI00704598.

PIR

A25268.

RefSeq

NP_776846.1.

UniGene

Bt.54

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FQJ	X-ray	2.02	C	46-87	[»]
2JU4	NMR	-	A	1-86	[»]

DisProt

DP00347.

ModBase

Search...

Genome annotation databases

Ensembl

ENSBTAG00000000354. Bos taurus. [Contig view]

GeneID

281977.

KEGG

bta:281977.

Phylogenomic databases

HOVERGEN

P04972.

Enzyme and pathway databases

BRENDA

3.1.4.17. 251.

Family and domain databases

InterPro

IPR006952. PDE6_gamma.
[Graphical view]

PANTHER

PTHR12122. PDE6_gamma. 1 hit.

Pfam

PF04868. PDE6_gamma. 1 hit.
[Graphical view]

PIRSF

PIRSF000969. 35-cGMP_Pdiase_g. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CNRG_BOVIN

Accession

Primary (citable) accession number: P04972

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)