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Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

gpd-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Miscellaneous

There are four nearly identical glyceraldehyde 3-phosphate dehydrogenases in C.elegans.

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd-2), Glyceraldehyde-3-phosphate dehydrogenase 4 (gpd-4), Glyceraldehyde-3-phosphate dehydrogenase 3 (gpd-3), Glyceraldehyde-3-phosphate dehydrogenase 1 (gpd-1)
  2. Probable phosphoglycerate kinase (pgk-1)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (ipgm-1)
  4. Enolase (enol-1)
  5. Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-2), Pyruvate kinase (pyk-1), Pyruvate kinase (pyk-1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADBy similarity1
Binding sitei85NAD; via carbonyl oxygenBy similarity1
Active sitei158NucleophilePROSITE-ProRule annotation1
Sitei185Activates thiol group during catalysisBy similarity1
Binding sitei188Glyceraldehyde 3-phosphateBy similarity1
Binding sitei240Glyceraldehyde 3-phosphateBy similarity1
Binding sitei322NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

ReactomeiR-CEL-70171. Glycolysis.
R-CEL-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1 (EC:1.2.1.12)
Short name:
GAPDH-1
Gene namesi
Name:gpd-1
ORF Names:T09F3.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiT09F3.3; CE02343; WBGene00001683; gpd-1.

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001455101 – 341Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST341

Proteomic databases

EPDiP04970.
PaxDbiP04970.
PeptideAtlasiP04970.
PRIDEiP04970.

2D gel databases

World-2DPAGEi0020:P04970.

Expressioni

Gene expression databases

BgeeiWBGene00001683.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi39922. 2 interactors.
DIPiDIP-24716N.
IntActiP04970. 2 interactors.
MINTiMINT-1097728.
STRINGi6239.T09F3.3.2.

Structurei

3D structure databases

ProteinModelPortaliP04970.
SMRiP04970.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 159Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni217 – 218Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0657. Eukaryota.
COG0057. LUCA.
GeneTreeiENSGT00760000119172.
HOGENOMiHOG000071678.
InParanoidiP04970.
KOiK00134.
OMAiVACPVKG.
OrthoDBiEOG091G0B1Y.
PhylomeDBiP04970.

Family and domain databases

InterProiView protein in InterPro
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiView protein in Pfam
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

P04970-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKANVGING FGRIGRLVLR AAVEKDTVQV VAVNDPFITI DYMVYLFKYD
60 70 80 90 100
STHGQFKGTV TYDGDFLIVQ KDGKSSHKIK VFNSKDPAAI AWGSVKADFV
110 120 130 140 150
VESTGVFTTK EKASAHLQGG AKKVIISAPS ADAPMYVVGV NHEKYDASND
160 170 180 190 200
HVVSNASCTT NCLAPLAKVI NDNFGIIEGL MTTVHAVTAT QKTVDGPSGK
210 220 230 240 250
LWRDGRGAGQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR VPTPDVSVVD
260 270 280 290 300
LTVRLEKPAS MDDIKKVVKA AADGPMKGIL AYTEDQVVST DFVSDPHSSI
310 320 330 340
FDAGACISLN PNFVKLVSWY DNEYGYSNRV VDLIGYIATR G
Length:341
Mass (Da):36,382
Last modified:August 13, 1987 - v1
Checksum:i648E40ED4BD270CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52674 Genomic DNA. Translation: CAA36900.1.
X04818 Genomic DNA. Translation: CAA28504.1.
Z49070 Genomic DNA. Translation: CAA88870.1.
PIRiS03911. DEKWG1.
RefSeqiNP_496237.1. NM_063836.4.
UniGeneiCel.6897.

Genome annotation databases

EnsemblMetazoaiT09F3.3.1; T09F3.3.1; WBGene00001683.
T09F3.3.2; T09F3.3.2; WBGene00001683.
GeneIDi174603.
KEGGicel:CELE_T09F3.3.
UCSCiT09F3.3.1. c. elegans.

Similar proteinsi

Entry informationi

Entry nameiG3P1_CAEEL
AccessioniPrimary (citable) accession number: P04970
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 25, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families