Glyceraldehyde-3-phosphate dehydrogenase 1

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Reviewed, UniProtKB/Swiss-Prot P04970 (G3P1_CAEEL)

Last modified November 3, 2009. Version 88. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 1
      Short name=GAPDH-1
    EC=1.2.1.12

Gene names

Name:	gpd-1
ORF Names:	T09F3.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

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Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD⁺ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	There are four nearly identical glyceraldehyde 3-phosphate dehydrogenases in C.elegans.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

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Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryonic development ending in birth or egg hatching Inferred from mutant phenotype. Source: WormBase glycolysis Inferred from electronic annotation. Source: UniProtKB-KW gonad development Inferred from mutant phenotype. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of multicellular organism growth Inferred from mutant phenotype. Source: WormBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
zyg-8	Q95QC4	1	EBI-326242,EBI-331795

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 341

341

Glyceraldehyde-3-phosphate dehydrogenase 1

PRO_0000145510

Regions

Nucleotide binding

13 – 14

NAD By similarity

Region

157 – 159

Glyceraldehyde 3-phosphate binding By similarity

Region

217 – 218

Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site

158

Nucleophile By similarity

Binding site

NAD By similarity

Binding site

NAD; via carbonyl oxygen By similarity

Binding site

188

Glyceraldehyde 3-phosphate By similarity

Binding site

240

Glyceraldehyde 3-phosphate By similarity

Binding site

322

NAD By similarity

Site

185

Activates thiol group during catalysis By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P04970-1 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 648E40ED4BD270CC
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FASTA

341

36,382

        10         20         30         40         50         60 
MSKANVGING FGRIGRLVLR AAVEKDTVQV VAVNDPFITI DYMVYLFKYD STHGQFKGTV 

        70         80         90        100        110        120 
TYDGDFLIVQ KDGKSSHKIK VFNSKDPAAI AWGSVKADFV VESTGVFTTK EKASAHLQGG 

       130        140        150        160        170        180 
AKKVIISAPS ADAPMYVVGV NHEKYDASND HVVSNASCTT NCLAPLAKVI NDNFGIIEGL 

       190        200        210        220        230        240 
MTTVHAVTAT QKTVDGPSGK LWRDGRGAGQ NIIPASTGAA KAVGKVIPEL NGKLTGMAFR 

       250        260        270        280        290        300 
VPTPDVSVVD LTVRLEKPAS MDDIKKVVKA AADGPMKGIL AYTEDQVVST DFVSDPHSSI 

       310        320        330        340 
FDAGACISLN PNFVKLVSWY DNEYGYSNRV VDLIGYIATR G

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization of the glyceraldehyde-3-phosphate dehydrogenase gene family of Caenorhabditis elegans." Huang X.Y., Barrios L.A.M., Vonkhorporn P., Honda S., Albertson D.G., Hecht R.M. J. Mol. Biol. 206:411-424(1989) [PubMed: 2716055] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bristol N2.
[2]	"The glyceraldehyde-3-phosphate dehydrogenase gene family in the nematode, Caenorhabditis elegans: isolation and characterization of one of the genes." Yarbrough P.O., Hayden M.A., Dunn L.A., Vermersch P.S., Klass M.R., Hecht R.M. Biochim. Biophys. Acta 908:21-33(1987) [PubMed: 3026481] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X52674 Genomic DNA. Translation: CAA36900.1. X04818 Genomic DNA. Translation: CAA28504.1. Z49070 Genomic DNA. Translation: CAA88870.1.
PIR	DEKWG1. S03911.
RefSeq	NP_496237.1.
UniGene	Cel.6897
3D structure databases
HSSP	HSSP built from PDB template 1CRW based on UniProtKB P56649.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24716N.
IntAct	P04970. 2 interactions.
STRING	P04970.
Genome annotation databases
Ensembl	T09F3.3.1; T09F3.3.1; T09F3.3; Caenorhabditis elegans. [Genome view] T09F3.3.2; T09F3.3.2; T09F3.3; Caenorhabditis elegans. [Genome view]
GeneID	174603.
KEGG	cel:T09F3.3.
UCSC	T09F3.3.1. c. elegans.
Organism-specific databases
CTD	174603.
WormBase	WBGene00001683. gpd-1.
WormPep	T09F3.3. CE02343. [WorfDB]
Phylogenomic databases
OMA	TVFHERD.
Enzyme and pathway databases
BRENDA	1.2.1.12. 672.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	884736.

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Entry information

Entry name

G3P1_CAEEL

Accession

Primary (citable) accession number: P04970

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	November 3, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project

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Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other Resources

Entry information

Relevant documents