Proliferating cell nuclear antigen - Rattus norvegicus (Rat)

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P04961 (PCNA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 99. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Proliferating cell nuclear antigen
Short name=PCNA

Alternative name(s):
Cyclin

Gene names

Name:

Pcna

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities By similarity. Has to be loaded onto DNA in order to be able to stimulate APEX2 By similarity.
Subunit structure	Homotrimer By similarity. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Forms a ternary complex with DNTTIP2 and core histone. Interacts with PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH By similarity. Interacts with KCTD10. Interacts with NUDT15 By similarity. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with CDKN1A/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA By similarity. Ref.3
Subcellular location	Nucleus By similarity. Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents By similarity.
Post-translational modification	Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis By similarity. Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation By similarity. Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA By similarity.
Sequence similarities	Belongs to the PCNA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW heart development Inferred from expression pattern. Source: RGD positive regulation of deoxyribonuclease activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of DNA replication Inferred from electronic annotation. Source: InterPro response to cadmium ion Inferred from expression pattern. Source: RGD response to lipid Inferred from direct assay. Source: RGD translesion synthesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	PCNA complex Inferred from electronic annotation. Source: InterPro PCNA-p21 complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	DNA polymerase processivity factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 261

261

Proliferating cell nuclear antigen

PRO_0000149161

Regions

DNA binding

61 – 80

Potential

Amino acid modifications

Modified residue

N6-acetyllysine By similarity

Modified residue

N6-acetyllysine By similarity

Modified residue

211

Phosphotyrosine; by EGFR By similarity

Modified residue

248

N6-acetyllysine By similarity

Cross-link

164

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P04961 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: CE55E9BA46C50F07
Show »

FASTA

261

28,749

        10         20         30         40         50         60 
MFEARLIQGS ILKKVLEALK DLINEACWDI SSGGVNLQSM DSSHVSLVQL TLRSEGFDTY 

        70         80         90        100        110        120 
RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD 

       130        140        150        160        170        180 
LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI 

       190        200        210        220        230        240 
KLSQTSNVDK EEEAVSIEMN EPVQLTFALR YLNFFTKATP LSPTVTLSMS ADVPLVVEYK 

       250        260 
IADMGHLKYY LAPKIEDEEG S

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of cDNA coding for rat proliferating cell nuclear antigen (PCNA)/cyclin." Matsumoto K., Moriuchi T., Koji T., Nakan P.K. EMBO J. 6:637-642(1987) [PubMed: 2884104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: W/FU.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	"A novel PDIP1-related protein, KCTD10, that interacts with proliferating cell nuclear antigen and DNA polymerase delta." Zhou J., Ren K., Liu X., Xiong X., Hu X., Zhang J. Biochim. Biophys. Acta 1729:200-203(2005) [PubMed: 15982757] [Abstract] Cited for: INTERACTION WITH KCTD10. Strain: Wistar.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00047 mRNA. Translation: CAA68261.1. BC060570 mRNA. Translation: AAH60570.1.
IPI	IPI00200861.
PIR	WMRTET. A26631.
RefSeq	NP_071776.1. NM_022381.3.
UniGene	Rn.223.
3D structure databases
ProteinModelPortal	P04961.
SMR	P04961. Positions 1-260.
ModBase	Search...
Protein-protein interaction databases
STRING	P04961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000028887; ENSRNOP00000028887; ENSRNOG00000021264.
GeneID	25737.
KEGG	rno:25737.
UCSC	NM_022381. rat.
Organism-specific databases
CTD	5111.
RGD	3269. Pcna.
Phylogenomic databases
eggNOG	roNOG14028.
GeneTree	ENSGT00390000004965.
HOVERGEN	HBG000947.
InParanoid	P04961.
OMA	KAQDNAD.
OrthoDB	EOG4933JJ.
PhylomeDB	P04961.
Gene expression databases
ArrayExpress	P04961.
Genevestigator	P04961.
GermOnline	ENSRNOG00000021264. Rattus norvegicus.
Family and domain databases
InterPro	IPR000730. Pr_cel_nuc_antig. IPR022649. Pr_cel_nuc_antig_C. IPR022659. Pr_cel_nuc_antig_CS. IPR022648. Pr_cel_nuc_antig_N. [Graphical view]
KO	K04802.
PANTHER	PTHR11352. Pr_cel_nuc_antig. 1 hit.
Pfam	PF02747. PCNA_C. 1 hit. PF00705. PCNA_N. 1 hit. [Graphical view]
PRINTS	PR00339. PCNACYCLIN.
TIGRFAMs	TIGR00590. Pcna. 1 hit.
PROSITE	PS01251. PCNA_1. 1 hit. PS00293. PCNA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607879.

Entry information

Entry name

PCNA_RAT

Accession

Primary (citable) accession number: P04961

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	December 14, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents