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Protein

Beta-glucanase

Gene

bglS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133NucleophilePROSITE-ProRule annotation1
Active sitei137Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciBSUB:BSU39070-MONOMER.
SABIO-RKP04957.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene namesi
Name:bglS
Synonyms:bgl, licS
Ordered Locus Names:BSU39070
ORF Names:N15B
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 281 PublicationAdd BLAST28
ChainiPRO_000001179129 – 242Beta-glucanaseAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29Pyrrolidone carboxylic acid1
Disulfide bondi60 ↔ 89By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP04957.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021091.

Structurei

Secondary structure

1242
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 36Combined sources3
Turni43 – 45Combined sources3
Beta strandi46 – 48Combined sources3
Beta strandi60 – 62Combined sources3
Helixi64 – 66Combined sources3
Beta strandi67 – 69Combined sources3
Beta strandi75 – 83Combined sources9
Beta strandi86 – 96Combined sources11
Beta strandi100 – 108Combined sources9
Beta strandi115 – 123Combined sources9
Helixi125 – 127Combined sources3
Beta strandi132 – 139Combined sources8
Beta strandi145 – 152Combined sources8
Beta strandi160 – 163Combined sources4
Helixi168 – 170Combined sources3
Beta strandi173 – 179Combined sources7
Beta strandi184 – 188Combined sources5
Beta strandi191 – 196Combined sources6
Beta strandi205 – 216Combined sources12
Helixi218 – 221Combined sources4
Beta strandi230 – 241Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3O5SX-ray2.20A29-242[»]
ProteinModelPortaliP04957.
SMRiP04957.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04957.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 242GH16PROSITE-ProRule annotationAdd BLAST214

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated
Contains 1 GH16 (glycosyl hydrolase family 16) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108RZ2. Bacteria.
COG2273. LUCA.
HOGENOMiHOG000250723.
InParanoidiP04957.
OMAiKIMMNAW.
PhylomeDBiP04957.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYLKRVLLL LVTGLFMSLF AVTATASAQT GGSFFDPFNG YNSGFWQKAD
60 70 80 90 100
GYSNGNMFNC TWRANNVSMT SLGEMRLALT SPAYNKFDCG ENRSVQTYGY
110 120 130 140 150
GLYEVRMKPA KNTGIVSSFF TYTGPTDGTP WDEIDIEFLG KDTTKVQFNY
160 170 180 190 200
YTNGAGNHEK IVDLGFDAAN AYHTYAFDWQ PNSIKWYVDG QLKHTATNQI
210 220 230 240
PTTPGKIMMN LWNGTGVDEW LGSYNGVNPL YAHYDWVRYT KK
Length:242
Mass (Da):27,269
Last modified:November 1, 1995 - v2
Checksum:i45958DEA70F22B29
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti24A → S in strain: HL-25. 1
Natural varianti83A → S in strain: HL-25. 1
Natural varianti204P → L in strain: C120. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00754 Genomic DNA. Translation: CAA25328.1.
D00518 Genomic DNA. Translation: BAA00405.1.
D83026 Genomic DNA. Translation: BAA11697.1.
Z46862 Genomic DNA. Translation: CAA86922.1.
AL009126 Genomic DNA. Translation: CAB15943.1.
Z28340 Genomic DNA. Translation: CAA82195.1.
PIRiI40370. LXBS.
RefSeqiNP_391786.1. NC_000964.3.
WP_003244531.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15943; CAB15943; BSU39070.
GeneIDi937470.
KEGGibsu:BSU39070.
PATRICi18979872. VBIBacSub10457_4098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00754 Genomic DNA. Translation: CAA25328.1.
D00518 Genomic DNA. Translation: BAA00405.1.
D83026 Genomic DNA. Translation: BAA11697.1.
Z46862 Genomic DNA. Translation: CAA86922.1.
AL009126 Genomic DNA. Translation: CAB15943.1.
Z28340 Genomic DNA. Translation: CAA82195.1.
PIRiI40370. LXBS.
RefSeqiNP_391786.1. NC_000964.3.
WP_003244531.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3O5SX-ray2.20A29-242[»]
ProteinModelPortaliP04957.
SMRiP04957.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021091.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbiP04957.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15943; CAB15943; BSU39070.
GeneIDi937470.
KEGGibsu:BSU39070.
PATRICi18979872. VBIBacSub10457_4098.

Phylogenomic databases

eggNOGiENOG4108RZ2. Bacteria.
COG2273. LUCA.
HOGENOMiHOG000250723.
InParanoidiP04957.
OMAiKIMMNAW.
PhylomeDBiP04957.

Enzyme and pathway databases

BioCyciBSUB:BSU39070-MONOMER.
SABIO-RKP04957.

Miscellaneous databases

EvolutionaryTraceiP04957.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUB_BACSU
AccessioniPrimary (citable) accession number: P04957
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
The sequence of strain 168 is shown.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.