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P04957

- GUB_BACSU

UniProt

P04957 - GUB_BACSU

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Protein

Beta-glucanase

Gene

bglS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei133 – 1331NucleophilePROSITE-ProRule annotation
Active sitei137 – 1371Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. licheninase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciBSUB:BSU39070-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene namesi
Name:bglS
Synonyms:bgl, licS
Ordered Locus Names:BSU39070
ORF Names:N15B
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU39070. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 28281 PublicationAdd
BLAST
Chaini29 – 242214Beta-glucanasePRO_0000011791Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei29 – 291Pyrrolidone carboxylic acid
Disulfide bondi60 ↔ 89By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP04957.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU39070.

Structurei

Secondary structure

1
242
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi34 – 363
Turni43 – 453
Beta strandi46 – 483
Beta strandi60 – 623
Helixi64 – 663
Beta strandi67 – 693
Beta strandi75 – 839
Beta strandi86 – 9611
Beta strandi100 – 1089
Beta strandi115 – 1239
Helixi125 – 1273
Beta strandi132 – 1398
Beta strandi145 – 1528
Beta strandi160 – 1634
Helixi168 – 1703
Beta strandi173 – 1797
Beta strandi184 – 1885
Beta strandi191 – 1966
Beta strandi205 – 21612
Helixi218 – 2214
Beta strandi230 – 24112

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3O5SX-ray2.20A29-242[»]
ProteinModelPortaliP04957.
SMRiP04957. Positions 29-242.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04957.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2273.
HOGENOMiHOG000250723.
InParanoidiP04957.
OMAiHTATTNI.
OrthoDBiEOG6JB18P.
PhylomeDBiP04957.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSiPR00737. GLHYDRLASE16.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04957-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPYLKRVLLL LVTGLFMSLF AVTATASAQT GGSFFDPFNG YNSGFWQKAD
60 70 80 90 100
GYSNGNMFNC TWRANNVSMT SLGEMRLALT SPAYNKFDCG ENRSVQTYGY
110 120 130 140 150
GLYEVRMKPA KNTGIVSSFF TYTGPTDGTP WDEIDIEFLG KDTTKVQFNY
160 170 180 190 200
YTNGAGNHEK IVDLGFDAAN AYHTYAFDWQ PNSIKWYVDG QLKHTATNQI
210 220 230 240
PTTPGKIMMN LWNGTGVDEW LGSYNGVNPL YAHYDWVRYT KK
Length:242
Mass (Da):27,269
Last modified:November 1, 1995 - v2
Checksum:i45958DEA70F22B29
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti24 – 241A → S in strain: HL-25.
Natural varianti83 – 831A → S in strain: HL-25.
Natural varianti204 – 2041P → L in strain: C120.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00754 Genomic DNA. Translation: CAA25328.1.
D00518 Genomic DNA. Translation: BAA00405.1.
D83026 Genomic DNA. Translation: BAA11697.1.
Z46862 Genomic DNA. Translation: CAA86922.1.
AL009126 Genomic DNA. Translation: CAB15943.1.
Z28340 Genomic DNA. Translation: CAA82195.1.
PIRiI40370. LXBS.
RefSeqiNP_391786.1. NC_000964.3.
WP_003244531.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB15943; CAB15943; BSU39070.
GeneIDi937470.
KEGGibsu:BSU39070.
PATRICi18979872. VBIBacSub10457_4098.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00754 Genomic DNA. Translation: CAA25328.1 .
D00518 Genomic DNA. Translation: BAA00405.1 .
D83026 Genomic DNA. Translation: BAA11697.1 .
Z46862 Genomic DNA. Translation: CAA86922.1 .
AL009126 Genomic DNA. Translation: CAB15943.1 .
Z28340 Genomic DNA. Translation: CAA82195.1 .
PIRi I40370. LXBS.
RefSeqi NP_391786.1. NC_000964.3.
WP_003244531.1. NZ_CM000487.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3O5S X-ray 2.20 A 29-242 [» ]
ProteinModelPortali P04957.
SMRi P04957. Positions 29-242.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU39070.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi P04957.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15943 ; CAB15943 ; BSU39070 .
GeneIDi 937470.
KEGGi bsu:BSU39070.
PATRICi 18979872. VBIBacSub10457_4098.

Organism-specific databases

GenoListi BSU39070. [Micado ]

Phylogenomic databases

eggNOGi COG2273.
HOGENOMi HOG000250723.
InParanoidi P04957.
OMAi HTATTNI.
OrthoDBi EOG6JB18P.
PhylomeDBi P04957.

Enzyme and pathway databases

BioCyci BSUB:BSU39070-MONOMER.

Miscellaneous databases

EvolutionaryTracei P04957.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR008264. Beta_glucanase.
IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
[Graphical view ]
PRINTSi PR00737. GLHYDRLASE16.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase."
    Murphy N., McConnell D.J., Cantwell B.A.
    Nucleic Acids Res. 12:5355-5367(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C120.
  2. "Construction of a beta-glucanase hyperproducing Bacillus subtilis using the cloned beta-glucanase gene and a multi-copy plasmid."
    Tezuka H., Yuuki T., Yabuuchi S.
    Agric. Biol. Chem. 53:2335-2339(1989)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: HL-25.
  3. "Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region."
    Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y.
    Microbiology 142:3113-3123(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / BGSC1A1.
  4. "Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase."
    Wolf M., Geczi A., Simon O., Borriss R.
    Microbiology 141:281-290(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  5. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  6. "LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family."
    Schnetz K., Stuelke J., Gertz S., Krueger S., Krieg M., Hecker M., Rak B.
    J. Bacteriol. 178:1971-1979(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
    Strain: 168 / BR151.
  7. "Purification and some properties of two enzymes from a beta-glucanase hyperproducing strain, Bacillus subtilis HL-25."
    Yuuki T., Tezuka H., Yabuuchi S.
    Agric. Biol. Chem. 53:2341-2346(1989)
    Cited for: PROTEIN SEQUENCE OF 29-63.

Entry informationi

Entry nameiGUB_BACSU
AccessioniPrimary (citable) accession number: P04957
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
The sequence of strain 168 is shown.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3