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P04957 (GUB_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-glucanase

EC=3.2.1.73
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene names
Name:bglS
Synonyms:bgl, licS
Ordered Locus Names:BSU39070
ORF Names:N15B
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Subcellular location

Secreted.

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

The sequence of strain 168 is shown.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Pyrrolidone carboxylic acid
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionlicheninase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.7
Chain29 – 242214Beta-glucanase
PRO_0000011791

Sites

Active site1331Nucleophile By similarity
Active site1371Proton donor By similarity

Amino acid modifications

Modified residue291Pyrrolidone carboxylic acid
Disulfide bond60 ↔ 89 By similarity

Natural variations

Natural variant241A → S in strain: HL-25.
Natural variant831A → S in strain: HL-25.
Natural variant2041P → L in strain: C120.

Secondary structure

......................................... 242
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04957 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 45958DEA70F22B29

FASTA24227,269
        10         20         30         40         50         60 
MPYLKRVLLL LVTGLFMSLF AVTATASAQT GGSFFDPFNG YNSGFWQKAD GYSNGNMFNC 

        70         80         90        100        110        120 
TWRANNVSMT SLGEMRLALT SPAYNKFDCG ENRSVQTYGY GLYEVRMKPA KNTGIVSSFF 

       130        140        150        160        170        180 
TYTGPTDGTP WDEIDIEFLG KDTTKVQFNY YTNGAGNHEK IVDLGFDAAN AYHTYAFDWQ 

       190        200        210        220        230        240 
PNSIKWYVDG QLKHTATNQI PTTPGKIMMN LWNGTGVDEW LGSYNGVNPL YAHYDWVRYT 


KK 

« Hide

References

« Hide 'large scale' references
[1]"The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase."
Murphy N., McConnell D.J., Cantwell B.A.
Nucleic Acids Res. 12:5355-5367(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C120.
[2]"Construction of a beta-glucanase hyperproducing Bacillus subtilis using the cloned beta-glucanase gene and a multi-copy plasmid."
Tezuka H., Yuuki T., Yabuuchi S.
Agric. Biol. Chem. 53:2335-2339(1989)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HL-25.
[3]"Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region."
Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y.
Microbiology 142:3113-3123(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / BGSC1A1.
[4]"Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase."
Wolf M., Geczi A., Simon O., Borriss R.
Microbiology 141:281-290(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[5]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[6]"LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family."
Schnetz K., Stuelke J., Gertz S., Krueger S., Krieg M., Hecker M., Rak B.
J. Bacteriol. 178:1971-1979(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
Strain: 168 / BR151.
[7]"Purification and some properties of two enzymes from a beta-glucanase hyperproducing strain, Bacillus subtilis HL-25."
Yuuki T., Tezuka H., Yabuuchi S.
Agric. Biol. Chem. 53:2341-2346(1989)
Cited for: PROTEIN SEQUENCE OF 29-63.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X00754 Genomic DNA. Translation: CAA25328.1.
D00518 Genomic DNA. Translation: BAA00405.1.
D83026 Genomic DNA. Translation: BAA11697.1.
Z46862 Genomic DNA. Translation: CAA86922.1.
AL009126 Genomic DNA. Translation: CAB15943.1.
Z28340 Genomic DNA. Translation: CAA82195.1.
PIRLXBS. I40370.
RefSeqNP_391786.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3O5SX-ray2.20A26-242[»]
ProteinModelPortalP04957.
SMRP04957. Positions 29-242.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU39070.

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbP04957.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15943; CAB15943; BSU39070.
GeneID937470.
KEGGbsu:BSU39070.
PATRIC18979872. VBIBacSub10457_4098.

Organism-specific databases

GenoListBSU39070. [Micado]

Phylogenomic databases

eggNOGCOG2273.
HOGENOMHOG000250723.
OMAAYNKFDC.
OrthoDBEOG6JB18P.
ProtClustDBCLSK883280.

Enzyme and pathway databases

BioCycBSUB:BSU39070-MONOMER.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008264. Beta_glucanase.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSPR00737. GLHYDRLASE16.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP04957.

Entry information

Entry nameGUB_BACSU
AccessionPrimary (citable) accession number: P04957
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: November 13, 2013
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList