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P04957

- GUB_BACSU

UniProt

P04957 - GUB_BACSU

Protein

Beta-glucanase

Gene

bglS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei133 – 1331NucleophilePROSITE-ProRule annotation
    Active sitei137 – 1371Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. licheninase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciBSUB:BSU39070-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-glucanase (EC:3.2.1.73)
    Alternative name(s):
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Endo-beta-1,3-1,4 glucanase
    Lichenase
    Gene namesi
    Name:bglS
    Synonyms:bgl, licS
    Ordered Locus Names:BSU39070
    ORF Names:N15B
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU39070. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 28281 PublicationAdd
    BLAST
    Chaini29 – 242214Beta-glucanasePRO_0000011791Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei29 – 291Pyrrolidone carboxylic acid
    Disulfide bondi60 ↔ 89By similarity

    Keywords - PTMi

    Disulfide bond, Pyrrolidone carboxylic acid

    Proteomic databases

    PaxDbiP04957.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU39070.

    Structurei

    Secondary structure

    1
    242
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi34 – 363
    Turni43 – 453
    Beta strandi46 – 483
    Beta strandi60 – 623
    Helixi64 – 663
    Beta strandi67 – 693
    Beta strandi75 – 839
    Beta strandi86 – 9611
    Beta strandi100 – 1089
    Beta strandi115 – 1239
    Helixi125 – 1273
    Beta strandi132 – 1398
    Beta strandi145 – 1528
    Beta strandi160 – 1634
    Helixi168 – 1703
    Beta strandi173 – 1797
    Beta strandi184 – 1885
    Beta strandi191 – 1966
    Beta strandi205 – 21612
    Helixi218 – 2214
    Beta strandi230 – 24112

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3O5SX-ray2.20A29-242[»]
    ProteinModelPortaliP04957.
    SMRiP04957. Positions 29-242.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04957.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 16 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2273.
    HOGENOMiHOG000250723.
    OMAiHTATTNI.
    OrthoDBiEOG6JB18P.
    PhylomeDBiP04957.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    [Graphical view]
    PRINTSiPR00737. GLHYDRLASE16.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04957-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPYLKRVLLL LVTGLFMSLF AVTATASAQT GGSFFDPFNG YNSGFWQKAD    50
    GYSNGNMFNC TWRANNVSMT SLGEMRLALT SPAYNKFDCG ENRSVQTYGY 100
    GLYEVRMKPA KNTGIVSSFF TYTGPTDGTP WDEIDIEFLG KDTTKVQFNY 150
    YTNGAGNHEK IVDLGFDAAN AYHTYAFDWQ PNSIKWYVDG QLKHTATNQI 200
    PTTPGKIMMN LWNGTGVDEW LGSYNGVNPL YAHYDWVRYT KK 242
    Length:242
    Mass (Da):27,269
    Last modified:November 1, 1995 - v2
    Checksum:i45958DEA70F22B29
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti24 – 241A → S in strain: HL-25.
    Natural varianti83 – 831A → S in strain: HL-25.
    Natural varianti204 – 2041P → L in strain: C120.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00754 Genomic DNA. Translation: CAA25328.1.
    D00518 Genomic DNA. Translation: BAA00405.1.
    D83026 Genomic DNA. Translation: BAA11697.1.
    Z46862 Genomic DNA. Translation: CAA86922.1.
    AL009126 Genomic DNA. Translation: CAB15943.1.
    Z28340 Genomic DNA. Translation: CAA82195.1.
    PIRiI40370. LXBS.
    RefSeqiNP_391786.1. NC_000964.3.
    WP_003244531.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB15943; CAB15943; BSU39070.
    GeneIDi937470.
    KEGGibsu:BSU39070.
    PATRICi18979872. VBIBacSub10457_4098.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X00754 Genomic DNA. Translation: CAA25328.1 .
    D00518 Genomic DNA. Translation: BAA00405.1 .
    D83026 Genomic DNA. Translation: BAA11697.1 .
    Z46862 Genomic DNA. Translation: CAA86922.1 .
    AL009126 Genomic DNA. Translation: CAB15943.1 .
    Z28340 Genomic DNA. Translation: CAA82195.1 .
    PIRi I40370. LXBS.
    RefSeqi NP_391786.1. NC_000964.3.
    WP_003244531.1. NZ_CM000487.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3O5S X-ray 2.20 A 29-242 [» ]
    ProteinModelPortali P04957.
    SMRi P04957. Positions 29-242.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU39070.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi P04957.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15943 ; CAB15943 ; BSU39070 .
    GeneIDi 937470.
    KEGGi bsu:BSU39070.
    PATRICi 18979872. VBIBacSub10457_4098.

    Organism-specific databases

    GenoListi BSU39070. [Micado ]

    Phylogenomic databases

    eggNOGi COG2273.
    HOGENOMi HOG000250723.
    OMAi HTATTNI.
    OrthoDBi EOG6JB18P.
    PhylomeDBi P04957.

    Enzyme and pathway databases

    BioCyci BSUB:BSU39070-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P04957.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008264. Beta_glucanase.
    IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    [Graphical view ]
    PRINTSi PR00737. GLHYDRLASE16.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase."
      Murphy N., McConnell D.J., Cantwell B.A.
      Nucleic Acids Res. 12:5355-5367(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C120.
    2. "Construction of a beta-glucanase hyperproducing Bacillus subtilis using the cloned beta-glucanase gene and a multi-copy plasmid."
      Tezuka H., Yuuki T., Yabuuchi S.
      Agric. Biol. Chem. 53:2335-2339(1989)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: HL-25.
    3. "Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region."
      Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y.
      Microbiology 142:3113-3123(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / BGSC1A1.
    4. "Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase."
      Wolf M., Geczi A., Simon O., Borriss R.
      Microbiology 141:281-290(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    5. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    6. "LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family."
      Schnetz K., Stuelke J., Gertz S., Krueger S., Krieg M., Hecker M., Rak B.
      J. Bacteriol. 178:1971-1979(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
      Strain: 168 / BR151.
    7. "Purification and some properties of two enzymes from a beta-glucanase hyperproducing strain, Bacillus subtilis HL-25."
      Yuuki T., Tezuka H., Yabuuchi S.
      Agric. Biol. Chem. 53:2341-2346(1989)
      Cited for: PROTEIN SEQUENCE OF 29-63.

    Entry informationi

    Entry nameiGUB_BACSU
    AccessioniPrimary (citable) accession number: P04957
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
    The sequence of strain 168 is shown.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3