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Reviewed, UniProtKB/Swiss-Prot P04957 (GUB_BACSU)

Last modified June 16, 2009. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-glucanase
    EC=3.2.1.73
Alternative name(s):
    Endo-beta-1,3-1,4 glucanase
    1,3-1,4-beta-D-glucan 4-glucanohydrolase
    Lichenase
Gene names
Name: bglS
Synonyms: bgl, licS
Ordered Locus Names: BSU39070
ORF Names: N15B
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length242 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Subcellular location

Secreted.

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.

The sequence of strain 168 is shown.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family.

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Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Pyrrolidone carboxylic acid
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionlicheninase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Ref.7
Chain29 – 242214Beta-glucanase
PRO_0000011791

Sites

Active site1331Nucleophile By similarity
Active site1371Proton donor By similarity

Amino acid modifications

Modified residue291Pyrrolidone carboxylic acid
Disulfide bond60 ↔ 89 By similarity

Natural variations

Natural variant241A → S in strain: HL-25.
Natural variant831A → S in strain: HL-25.
Natural variant2041P → L in strain: C120.

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Sequences

Sequence LengthMass (Da)Tools
P04957-1 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 45958DEA70F22B29

FASTA24227,269
        10         20         30         40         50         60 
MPYLKRVLLL LVTGLFMSLF AVTATASAQT GGSFFDPFNG YNSGFWQKAD GYSNGNMFNC 

        70         80         90        100        110        120 
TWRANNVSMT SLGEMRLALT SPAYNKFDCG ENRSVQTYGY GLYEVRMKPA KNTGIVSSFF 

       130        140        150        160        170        180 
TYTGPTDGTP WDEIDIEFLG KDTTKVQFNY YTNGAGNHEK IVDLGFDAAN AYHTYAFDWQ 

       190        200        210        220        230        240 
PNSIKWYVDG QLKHTATNQI PTTPGKIMMN LWNGTGVDEW LGSYNGVNPL YAHYDWVRYT 


KK

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References

« Hide 'large scale' references
[1]"The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase."
Murphy N., McConnell D.J., Cantwell B.A.
Nucleic Acids Res. 12:5355-5367(1984) [PubMed: 6087283] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: C120.
[2]"Construction of a beta-glucanase hyperproducing Bacillus subtilis using the cloned beta-glucanase gene and a multi-copy plasmid."
Tezuka H., Yuuki T., Yabuuchi S.
Agric. Biol. Chem. 53:2335-2339(1989)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: HL-25.
[3]"Sequencing of a 65 kb region of the Bacillus subtilis genome containing the lic and cel loci, and creation of a 177 kb contig covering the gnt-sacXY region."
Yoshida K., Shindo K., Sano H., Seki S., Fujimura M., Yanai N., Miwa Y., Fujita Y.
Microbiology 142:3113-3123(1996) [PubMed: 8969509] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / BGSC1A1.
[4]"Genes encoding xylan and beta-glucan hydrolysing enzymes in Bacillus subtilis: characterization, mapping and construction of strains deficient in lichenase, cellulase and xylanase."
Wolf M., Geczi A., Simon O., Borriss R.
Microbiology 141:281-290(1995) [PubMed: 7704256] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[5]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[6]"LicT, a Bacillus subtilis transcriptional antiterminator protein of the BglG family."
Schnetz K., Stuelke J., Gertz S., Krueger S., Krieg M., Hecker M., Rak B.
J. Bacteriol. 178:1971-1979(1996) [PubMed: 8606172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-6.
Strain: 168 / BR151.
[7]"Purification and some properties of two enzymes from a beta-glucanase hyperproducing strain, Bacillus subtilis HL-25."
Yuuki T., Tezuka H., Yabuuchi S.
Agric. Biol. Chem. 53:2341-2346(1989)
Cited for: PROTEIN SEQUENCE OF 29-63.

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Cross-references

Sequence databases

X00754 Genomic DNA. Translation: CAA25328.1.
D00518 Genomic DNA. Translation: BAA00405.1.
D83026 Genomic DNA. Translation: BAA11697.1.
Z46862 Genomic DNA. Translation: CAA86922.1.
AL009126 Genomic DNA. Translation: CAB15943.1.
Z28340 Genomic DNA. Translation: CAA82195.1.
PIRLXBS. I40370.
RefSeqNP_391786.1.

3D structure databases

HSSPHSSP built from PDB template 1GBG based on UniProtKB P27051.
SMRP04957. Positions 29-242.
ModBaseSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Genome annotation databases

GeneID937470.
GenomeReviewsGene locus BSU39070 in contig AL009126_GR.
KEGGbsu:BSU39070.
NMPDRfig|224308.1.peg.3912.

Organism-specific databases

SubtiListBG10476. bglS. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMP04957.
OMAP04957. AFFTYIG.

Enzyme and pathway databases

BioCycBSUB224308:BSU3903-MON.
BRENDA3.2.1.73. 150.

Family and domain databases

InterProIPR008264. Beta_glucanase.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
[Graphical view]
Gene3DG3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
PfamPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
PRINTSPR00737. GLHYDRLASE16.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGUB_BACSU
AccessionPrimary (citable) accession number: P04957
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: June 16, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents