Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-glucanase

Gene

bglS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Beta-glucanases of Bacillus have a substrate range similar to lichenase of germinating barley.
The sequence of strain 168 is shown.

Catalytic activityi

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei133NucleophilePROSITE-ProRule annotation1
Active sitei137Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciBSUB:BSU39070-MONOMER
SABIO-RKP04957

Protein family/group databases

CAZyiGH16 Glycoside Hydrolase Family 16

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-glucanase (EC:3.2.1.73)
Alternative name(s):
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene namesi
Name:bglS
Synonyms:bgl, licS
Ordered Locus Names:BSU39070
ORF Names:N15B
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 281 PublicationAdd BLAST28
ChainiPRO_000001179129 – 242Beta-glucanaseAdd BLAST214

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei29Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi60 ↔ 89By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP04957
PRIDEiP04957

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021091

Structurei

Secondary structure

1242
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi34 – 36Combined sources3
Turni43 – 45Combined sources3
Beta strandi46 – 48Combined sources3
Beta strandi60 – 62Combined sources3
Helixi64 – 66Combined sources3
Beta strandi67 – 69Combined sources3
Beta strandi75 – 83Combined sources9
Beta strandi86 – 96Combined sources11
Beta strandi100 – 108Combined sources9
Beta strandi115 – 123Combined sources9
Helixi125 – 127Combined sources3
Beta strandi132 – 139Combined sources8
Beta strandi145 – 152Combined sources8
Beta strandi160 – 163Combined sources4
Helixi168 – 170Combined sources3
Beta strandi173 – 179Combined sources7
Beta strandi184 – 188Combined sources5
Beta strandi191 – 196Combined sources6
Beta strandi205 – 216Combined sources12
Helixi218 – 221Combined sources4
Beta strandi230 – 241Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3O5SX-ray2.20A29-242[»]
ProteinModelPortaliP04957
SMRiP04957
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04957

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 242GH16PROSITE-ProRule annotationAdd BLAST214

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108RZ2 Bacteria
COG2273 LUCA
HOGENOMiHOG000250723
InParanoidiP04957
OMAiWYVSDGW
PhylomeDBiP04957

Family and domain databases

InterProiView protein in InterPro
IPR008264 Beta_glucanase
IPR013320 ConA-like_dom_sf
IPR000757 GH16
IPR008263 GH16_AS
PfamiView protein in Pfam
PF00722 Glyco_hydro_16, 1 hit
PRINTSiPR00737 GLHYDRLASE16
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS01034 GH16_1, 1 hit
PS51762 GH16_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYLKRVLLL LVTGLFMSLF AVTATASAQT GGSFFDPFNG YNSGFWQKAD
60 70 80 90 100
GYSNGNMFNC TWRANNVSMT SLGEMRLALT SPAYNKFDCG ENRSVQTYGY
110 120 130 140 150
GLYEVRMKPA KNTGIVSSFF TYTGPTDGTP WDEIDIEFLG KDTTKVQFNY
160 170 180 190 200
YTNGAGNHEK IVDLGFDAAN AYHTYAFDWQ PNSIKWYVDG QLKHTATNQI
210 220 230 240
PTTPGKIMMN LWNGTGVDEW LGSYNGVNPL YAHYDWVRYT KK
Length:242
Mass (Da):27,269
Last modified:November 1, 1995 - v2
Checksum:i45958DEA70F22B29
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti24A → S in strain: HL-25. 1
Natural varianti83A → S in strain: HL-25. 1
Natural varianti204P → L in strain: C120. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00754 Genomic DNA Translation: CAA25328.1
D00518 Genomic DNA Translation: BAA00405.1
D83026 Genomic DNA Translation: BAA11697.1
Z46862 Genomic DNA Translation: CAA86922.1
AL009126 Genomic DNA Translation: CAB15943.1
Z28340 Genomic DNA Translation: CAA82195.1
PIRiI40370 LXBS
RefSeqiNP_391786.1, NC_000964.3
WP_003244531.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB15943; CAB15943; BSU39070
GeneIDi937470
KEGGibsu:BSU39070
PATRICifig|224308.179.peg.4230

Similar proteinsi

Entry informationi

Entry nameiGUB_BACSU
AccessioniPrimary (citable) accession number: P04957
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health