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P04956

- GUNB_CLOTH

UniProt

P04956 - GUNB_CLOTH

Protein

Endoglucanase B

Gene

celB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei204 – 2041Proton donorBy similarity
    Active sitei363 – 3631NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Enzyme and pathway databases

    BioCyciCTHE203119:GIW8-554-MONOMER.
    MetaCyc:MONOMER-16415.

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase B (EC:3.2.1.4)
    Alternative name(s):
    Cellulase B
    Endo-1,4-beta-glucanase B
    Short name:
    EgB
    Gene namesi
    Name:celB
    Ordered Locus Names:Cthe_0536
    OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
    Taxonomic identifieri203119 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
    ProteomesiUP000002145: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Or 31Add
    BLAST
    Chaini28 – 563536Endoglucanase BPRO_0000007850Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi203119.Cthe_0536.

    Structurei

    3D structure databases

    ProteinModelPortaliP04956.
    SMRiP04956. Positions 497-563.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati502 – 526251Add
    BLAST
    Repeati534 – 557242Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni502 – 557562 X 24 AA approximate repeatsAdd
    BLAST

    Domaini

    A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG2730.
    HOGENOMiHOG000225207.
    OMAiWAYIMEN.
    OrthoDBiEOG6C8MT6.

    Family and domain databases

    Gene3Di1.10.1330.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04956-1 [UniParc]FASTAAdd to Basket

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    MKKFLVLLIA LIMIATLLVV PGVQTSAEGS YADLAEPDDD WLHVEGTNIV    50
    DKYGNKVWIT GANWFGFNCR ERMLLDSYHS DIIADIELVA DKGINVVRMP 100
    IATDLLYAWS QGIYPPSTDT SYNNPALAGL NSYELFNFML ENFKRVGIKV 150
    ILDVHSPETD NQGHNYPLWY NTTITEEIFK KAWVWVAERY KNDDTIIGFD 200
    LKNEPHTNTG TMKIKAQSAI WDDSNHPNNW KRVAEETALA ILEVHPNVLI 250
    FVEGVEMYPK DGIWDDETFD TSPWTGNNDY YGNWWGGNLR GVKDYPINLG 300
    KYQSQLVYSP HDYGPIVYEQ DWFKGDFITA NDEQAKRILY EQCWRDNWAY 350
    IMEEGISPLL LGEWGGMTEG GHPLLDLNLK YLRCMRDFIL ENKYKLHHTF 400
    WCINIDSADT GGLFTRDEGT PFPGGRDLKW NDNKYDNYLY PVLWKTEDGK 450
    FIGLDHKIPL GRNGISISQL SNYTPSVTPS PSATPSPTTI TAPPTDTVTY 500
    GDVNGDGRVN SSDVALLKRY LLGLVENINK EAADVNVSGT VNSTDLAIMK 550
    RYVLRSISEL PYK 563
    Length:563
    Mass (Da):63,929
    Last modified:August 13, 1987 - v1
    Checksum:i866FE55704A1DE4B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03592 Genomic DNA. Translation: CAA27266.1.
    CP000568 Genomic DNA. Translation: ABN51772.1.
    PIRiA23512. CZCLBM.
    RefSeqiWP_003517581.1. NC_009012.1.
    YP_001036965.1. NC_009012.1.

    Genome annotation databases

    EnsemblBacteriaiABN51772; ABN51772; Cthe_0536.
    GeneIDi4808285.
    KEGGicth:Cthe_0536.
    PATRICi19514731. VBICloThe47081_0558.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X03592 Genomic DNA. Translation: CAA27266.1 .
    CP000568 Genomic DNA. Translation: ABN51772.1 .
    PIRi A23512. CZCLBM.
    RefSeqi WP_003517581.1. NC_009012.1.
    YP_001036965.1. NC_009012.1.

    3D structure databases

    ProteinModelPortali P04956.
    SMRi P04956. Positions 497-563.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 203119.Cthe_0536.

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    DNASUi 4808285.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABN51772 ; ABN51772 ; Cthe_0536 .
    GeneIDi 4808285.
    KEGGi cth:Cthe_0536.
    PATRICi 19514731. VBICloThe47081_0558.

    Phylogenomic databases

    eggNOGi COG2730.
    HOGENOMi HOG000225207.
    OMAi WAYIMEN.
    OrthoDBi EOG6C8MT6.

    Enzyme and pathway databases

    BioCyci CTHE203119:GIW8-554-MONOMER.
    MetaCyc:MONOMER-16415.

    Family and domain databases

    Gene3Di 1.10.1330.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR016134. Cellulos_enz_dockerin_1.
    IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
    IPR018242. Dockerin_1.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    PF00404. Dockerin_1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF63446. SSF63446. 1 hit.
    PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
    PS00018. EF_HAND_1. 1 hit.
    PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B."
      Grepinet O., Beguin P.
      Nucleic Acids Res. 14:1791-1799(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

    Entry informationi

    Entry nameiGUNB_CLOTH
    AccessioniPrimary (citable) accession number: P04956
    Secondary accession number(s): A3DCU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3