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P04956

- GUNB_CLOTH

UniProt

P04956 - GUNB_CLOTH

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Protein

Endoglucanase B

Gene

celB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei204 – 2041Proton donorBy similarity
Active sitei363 – 3631NucleophileBy similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-554-MONOMER.
MetaCyc:MONOMER-16415.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Short name:
EgB
Gene namesi
Name:celB
Ordered Locus Names:Cthe_0536
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
ProteomesiUP000002145: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Or 31Add
BLAST
Chaini28 – 563536Endoglucanase BPRO_0000007850Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0536.

Structurei

3D structure databases

ProteinModelPortaliP04956.
SMRiP04956. Positions 497-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati502 – 526251Add
BLAST
Repeati534 – 557242Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni502 – 557562 X 24 AA approximate repeatsAdd
BLAST

Domaini

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG2730.
HOGENOMiHOG000225207.
OMAiWAYIMEN.
OrthoDBiEOG6C8MT6.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04956-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKFLVLLIA LIMIATLLVV PGVQTSAEGS YADLAEPDDD WLHVEGTNIV
60 70 80 90 100
DKYGNKVWIT GANWFGFNCR ERMLLDSYHS DIIADIELVA DKGINVVRMP
110 120 130 140 150
IATDLLYAWS QGIYPPSTDT SYNNPALAGL NSYELFNFML ENFKRVGIKV
160 170 180 190 200
ILDVHSPETD NQGHNYPLWY NTTITEEIFK KAWVWVAERY KNDDTIIGFD
210 220 230 240 250
LKNEPHTNTG TMKIKAQSAI WDDSNHPNNW KRVAEETALA ILEVHPNVLI
260 270 280 290 300
FVEGVEMYPK DGIWDDETFD TSPWTGNNDY YGNWWGGNLR GVKDYPINLG
310 320 330 340 350
KYQSQLVYSP HDYGPIVYEQ DWFKGDFITA NDEQAKRILY EQCWRDNWAY
360 370 380 390 400
IMEEGISPLL LGEWGGMTEG GHPLLDLNLK YLRCMRDFIL ENKYKLHHTF
410 420 430 440 450
WCINIDSADT GGLFTRDEGT PFPGGRDLKW NDNKYDNYLY PVLWKTEDGK
460 470 480 490 500
FIGLDHKIPL GRNGISISQL SNYTPSVTPS PSATPSPTTI TAPPTDTVTY
510 520 530 540 550
GDVNGDGRVN SSDVALLKRY LLGLVENINK EAADVNVSGT VNSTDLAIMK
560
RYVLRSISEL PYK
Length:563
Mass (Da):63,929
Last modified:August 13, 1987 - v1
Checksum:i866FE55704A1DE4B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03592 Genomic DNA. Translation: CAA27266.1.
CP000568 Genomic DNA. Translation: ABN51772.1.
PIRiA23512. CZCLBM.
RefSeqiWP_003517581.1. NC_009012.1.
YP_001036965.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51772; ABN51772; Cthe_0536.
GeneIDi4808285.
KEGGicth:Cthe_0536.
PATRICi19514731. VBICloThe47081_0558.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03592 Genomic DNA. Translation: CAA27266.1 .
CP000568 Genomic DNA. Translation: ABN51772.1 .
PIRi A23512. CZCLBM.
RefSeqi WP_003517581.1. NC_009012.1.
YP_001036965.1. NC_009012.1.

3D structure databases

ProteinModelPortali P04956.
SMRi P04956. Positions 497-563.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 203119.Cthe_0536.

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

DNASUi 4808285.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABN51772 ; ABN51772 ; Cthe_0536 .
GeneIDi 4808285.
KEGGi cth:Cthe_0536.
PATRICi 19514731. VBICloThe47081_0558.

Phylogenomic databases

eggNOGi COG2730.
HOGENOMi HOG000225207.
OMAi WAYIMEN.
OrthoDBi EOG6C8MT6.

Enzyme and pathway databases

BioCyci CTHE203119:GIW8-554-MONOMER.
MetaCyc:MONOMER-16415.

Family and domain databases

Gene3Di 1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEi PS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B."
    Grepinet O., Beguin P.
    Nucleic Acids Res. 14:1791-1799(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372.

Entry informationi

Entry nameiGUNB_CLOTH
AccessioniPrimary (citable) accession number: P04956
Secondary accession number(s): A3DCU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: October 1, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3