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P04956 (GUNB_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase B

EC=3.2.1.4
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Short name=EgB
Gene names
Name:celB
Ordered Locus Names:Cthe_0536
OrganismClostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]
Taxonomic identifier203119 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length563 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Domain

A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727Or 31
Chain28 – 563536Endoglucanase B
PRO_0000007850

Regions

Repeat502 – 526251
Repeat534 – 557242
Region502 – 557562 X 24 AA approximate repeats

Sites

Active site2041Proton donor By similarity
Active site3631Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P04956 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 866FE55704A1DE4B

FASTA56363,929
        10         20         30         40         50         60 
MKKFLVLLIA LIMIATLLVV PGVQTSAEGS YADLAEPDDD WLHVEGTNIV DKYGNKVWIT 

        70         80         90        100        110        120 
GANWFGFNCR ERMLLDSYHS DIIADIELVA DKGINVVRMP IATDLLYAWS QGIYPPSTDT 

       130        140        150        160        170        180 
SYNNPALAGL NSYELFNFML ENFKRVGIKV ILDVHSPETD NQGHNYPLWY NTTITEEIFK 

       190        200        210        220        230        240 
KAWVWVAERY KNDDTIIGFD LKNEPHTNTG TMKIKAQSAI WDDSNHPNNW KRVAEETALA 

       250        260        270        280        290        300 
ILEVHPNVLI FVEGVEMYPK DGIWDDETFD TSPWTGNNDY YGNWWGGNLR GVKDYPINLG 

       310        320        330        340        350        360 
KYQSQLVYSP HDYGPIVYEQ DWFKGDFITA NDEQAKRILY EQCWRDNWAY IMEEGISPLL 

       370        380        390        400        410        420 
LGEWGGMTEG GHPLLDLNLK YLRCMRDFIL ENKYKLHHTF WCINIDSADT GGLFTRDEGT 

       430        440        450        460        470        480 
PFPGGRDLKW NDNKYDNYLY PVLWKTEDGK FIGLDHKIPL GRNGISISQL SNYTPSVTPS 

       490        500        510        520        530        540 
PSATPSPTTI TAPPTDTVTY GDVNGDGRVN SSDVALLKRY LLGLVENINK EAADVNVSGT 

       550        560 
VNSTDLAIMK RYVLRSISEL PYK 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B."
Grepinet O., Beguin P.
Nucleic Acids Res. 14:1791-1799(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete sequence of Clostridium thermocellum ATCC 27405."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P.
Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X03592 Genomic DNA. Translation: CAA27266.1.
CP000568 Genomic DNA. Translation: ABN51772.1.
PIRCZCLBM. A23512.
RefSeqYP_001036965.1. NC_009012.1.

3D structure databases

ProteinModelPortalP04956.
SMRP04956. Positions 497-563.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING203119.Cthe_0536.

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

DNASU4808285.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABN51772; ABN51772; Cthe_0536.
GeneID4808285.
KEGGcth:Cthe_0536.
PATRIC19514731. VBICloThe47081_0558.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2730.
HOGENOMHOG000225207.
OMAWAYIMEN.
OrthoDBEOG6C8MT6.
ProtClustDBCLSK2785588.

Enzyme and pathway databases

BioCycCTHE203119:GIW8-554-MONOMER.
MetaCyc:MONOMER-16415.

Family and domain databases

Gene3D1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR016134. Cellulos_enz_dockerin_1.
IPR002105. Cellulos_enz_dockerin_1_Ca-bd.
IPR018242. Dockerin_1.
IPR018247. EF_Hand_1_Ca_BS.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS00018. EF_HAND_1. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUNB_CLOTH
AccessionPrimary (citable) accession number: P04956
Secondary accession number(s): A3DCU3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: February 19, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries