P04956 (GUNB_CLOTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase B
EC=3.2.1.4

Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Short name=EgB

Gene names

Name:	celB
Ordered Locus Names:	Cthe_0536

Organism

Clostridium thermocellum (strain ATCC 27405 / DSM 1237) [Complete proteome] [HAMAP]

Taxonomic identifier

203119 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium thermocellum

Protein attributes

Sequence length	563 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Domain	A 24 residue domain is repeated twice in this enzyme as well as in other C.thermocellum cellulosome enzymes. This domain may function as the binding ligand for the SL component.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Or 31
	Chain	28 – 563	536	Endoglucanase B		PRO_0000007850
Regions
	Repeat	502 – 526	25	1
	Repeat	534 – 557	24	2
	Region	502 – 557	56	2 X 24 AA approximate repeats
Sites
	Active site	204	1	Proton donor By similarity
	Active site	363	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04956 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 866FE55704A1DE4B	FASTA	563	63,929
	10 20 30 40 50 60 MKKFLVLLIA LIMIATLLVV PGVQTSAEGS YADLAEPDDD WLHVEGTNIV DKYGNKVWIT 70 80 90 100 110 120 GANWFGFNCR ERMLLDSYHS DIIADIELVA DKGINVVRMP IATDLLYAWS QGIYPPSTDT 130 140 150 160 170 180 SYNNPALAGL NSYELFNFML ENFKRVGIKV ILDVHSPETD NQGHNYPLWY NTTITEEIFK 190 200 210 220 230 240 KAWVWVAERY KNDDTIIGFD LKNEPHTNTG TMKIKAQSAI WDDSNHPNNW KRVAEETALA 250 260 270 280 290 300 ILEVHPNVLI FVEGVEMYPK DGIWDDETFD TSPWTGNNDY YGNWWGGNLR GVKDYPINLG 310 320 330 340 350 360 KYQSQLVYSP HDYGPIVYEQ DWFKGDFITA NDEQAKRILY EQCWRDNWAY IMEEGISPLL 370 380 390 400 410 420 LGEWGGMTEG GHPLLDLNLK YLRCMRDFIL ENKYKLHHTF WCINIDSADT GGLFTRDEGT 430 440 450 460 470 480 PFPGGRDLKW NDNKYDNYLY PVLWKTEDGK FIGLDHKIPL GRNGISISQL SNYTPSVTPS 490 500 510 520 530 540 PSATPSPTTI TAPPTDTVTY GDVNGDGRVN SSDVALLKRY LLGLVENINK EAADVNVSGT 550 560 VNSTDLAIMK RYVLRSISEL PYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the cellulase gene of Clostridium thermocellum coding for endoglucanase B." Grepinet O., Beguin P. Nucleic Acids Res. 14:1791-1799(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of Clostridium thermocellum ATCC 27405." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Wu J.H.D., Newcomb M., Richardson P. Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27405 / DSM 1237.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X03592 Genomic DNA. Translation: CAA27266.1. CP000568 Genomic DNA. Translation: ABN51772.1.
PIR	CZCLBM. A23512.
RefSeq	YP_001036965.1. NC_009012.1.
3D structure databases
ProteinModelPortal	P04956.
SMR	P04956. Positions 497-563.
ModBase	Search...
Protein-protein interaction databases
STRING	203119.Cthe_0536.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
DNASU	4808285.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABN51772; ABN51772; Cthe_0536.
GeneID	4808285.
KEGG	cth:Cthe_0536.
PATRIC	19514731. VBICloThe47081_0558.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2730.
HOGENOM	HOG000225207.
OMA	TIQGIAW.
ProtClustDB	CLSK2785588.
Enzyme and pathway databases
BioCyc	CTHE203119:GIW8-554-MONOMER. MetaCyc:MONOMER-16415.
Family and domain databases
Gene3D	1.10.1330.10. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR016134. Cellulos_enz_dockerin_1. IPR002105. Cellulos_enz_dockerin_1_Ca-bd. IPR018242. Dockerin_1. IPR018247. EF_Hand_1_Ca_BS. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. PF00404. Dockerin_1. 2 hits. [Graphical view]
SUPFAM	SSF63446. Cellulos_enz_dockerin_1. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00448. CLOS_CELLULOSOME_RPT. 2 hits. PS00018. EF_HAND_1. 1 hit. Uncertain. PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUNB_CLOTH

Accession

Primary (citable) accession number: P04956
Secondary accession number(s): A3DCU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program