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Protein

Endoglucanase B

Gene

celB

Organism
Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This enzyme catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei204 – 2041Proton donorBy similarity
Active sitei363 – 3631NucleophileBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-554-MONOMER.
MetaCyc:MONOMER-16415.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase B (EC:3.2.1.4)
Alternative name(s):
Cellulase B
Endo-1,4-beta-glucanase B
Short name:
EgB
Gene namesi
Name:celB
Ordered Locus Names:Cthe_0536
OrganismiClostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium thermocellum)
Taxonomic identifieri203119 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesRuminococcaceaeRuminiclostridium
Proteomesi
  • UP000002145 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Or 31Add
BLAST
Chaini28 – 563536Endoglucanase BPRO_0000007850Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi203119.Cthe_0536.

Structurei

3D structure databases

ProteinModelPortaliP04956.
SMRiP04956. Positions 497-563.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini496 – 56267DockerinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 dockerin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107FVX. Bacteria.
COG2730. LUCA.
HOGENOMiHOG000225207.
OMAiETDNMGH.
OrthoDBiPOG091H07QZ.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04956-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFLVLLIA LIMIATLLVV PGVQTSAEGS YADLAEPDDD WLHVEGTNIV
60 70 80 90 100
DKYGNKVWIT GANWFGFNCR ERMLLDSYHS DIIADIELVA DKGINVVRMP
110 120 130 140 150
IATDLLYAWS QGIYPPSTDT SYNNPALAGL NSYELFNFML ENFKRVGIKV
160 170 180 190 200
ILDVHSPETD NQGHNYPLWY NTTITEEIFK KAWVWVAERY KNDDTIIGFD
210 220 230 240 250
LKNEPHTNTG TMKIKAQSAI WDDSNHPNNW KRVAEETALA ILEVHPNVLI
260 270 280 290 300
FVEGVEMYPK DGIWDDETFD TSPWTGNNDY YGNWWGGNLR GVKDYPINLG
310 320 330 340 350
KYQSQLVYSP HDYGPIVYEQ DWFKGDFITA NDEQAKRILY EQCWRDNWAY
360 370 380 390 400
IMEEGISPLL LGEWGGMTEG GHPLLDLNLK YLRCMRDFIL ENKYKLHHTF
410 420 430 440 450
WCINIDSADT GGLFTRDEGT PFPGGRDLKW NDNKYDNYLY PVLWKTEDGK
460 470 480 490 500
FIGLDHKIPL GRNGISISQL SNYTPSVTPS PSATPSPTTI TAPPTDTVTY
510 520 530 540 550
GDVNGDGRVN SSDVALLKRY LLGLVENINK EAADVNVSGT VNSTDLAIMK
560
RYVLRSISEL PYK
Length:563
Mass (Da):63,929
Last modified:August 13, 1987 - v1
Checksum:i866FE55704A1DE4B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03592 Genomic DNA. Translation: CAA27266.1.
CP000568 Genomic DNA. Translation: ABN51772.1.
PIRiA23512. CZCLBM.
RefSeqiWP_003517581.1. NC_009012.1.

Genome annotation databases

EnsemblBacteriaiABN51772; ABN51772; Cthe_0536.
KEGGicth:Cthe_0536.
PATRICi19514731. VBICloThe47081_0558.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X03592 Genomic DNA. Translation: CAA27266.1.
CP000568 Genomic DNA. Translation: ABN51772.1.
PIRiA23512. CZCLBM.
RefSeqiWP_003517581.1. NC_009012.1.

3D structure databases

ProteinModelPortaliP04956.
SMRiP04956. Positions 497-563.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi203119.Cthe_0536.

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

DNASUi4808285.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABN51772; ABN51772; Cthe_0536.
KEGGicth:Cthe_0536.
PATRICi19514731. VBICloThe47081_0558.

Phylogenomic databases

eggNOGiENOG4107FVX. Bacteria.
COG2730. LUCA.
HOGENOMiHOG000225207.
OMAiETDNMGH.
OrthoDBiPOG091H07QZ.

Enzyme and pathway databases

BioCyciCTHE203119:GIW8-554-MONOMER.
MetaCyc:MONOMER-16415.

Family and domain databases

Gene3Di1.10.1330.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR002105. Dockerin_1_rpt.
IPR016134. Dockerin_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00150. Cellulase. 1 hit.
PF00404. Dockerin_1. 2 hits.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF63446. SSF63446. 1 hit.
PROSITEiPS00448. CLOS_CELLULOSOME_RPT. 2 hits.
PS51766. DOCKERIN. 1 hit.
PS00018. EF_HAND_1. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUNB_CLOTH
AccessioniPrimary (citable) accession number: P04956
Secondary accession number(s): A3DCU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.