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Protein

Opsin Rh3

Gene

Rh3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.

GO - Molecular functioni

GO - Biological processi

  • absorption of UV light Source: FlyBase
  • detection of UV Source: FlyBase
  • G-protein coupled receptor signaling pathway Source: FlyBase
  • phototransduction Source: FlyBase
  • phototransduction, UV Source: FlyBase
  • protein-chromophore linkage Source: UniProtKB-KW
  • visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Chromophore

Enzyme and pathway databases

ReactomeiR-DME-2187335. The retinoid cycle in cones (daylight vision).
R-DME-2453902. The canonical retinoid cycle in rods (twilight vision).
R-DME-2485179. Activation of the phototransduction cascade.
R-DME-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-DME-419771. Opsins.
R-DME-5620916. VxPx cargo-targeting to cilium.

Names & Taxonomyi

Protein namesi
Recommended name:
Opsin Rh3
Alternative name(s):
Inner R7 photoreceptor cells opsin
Gene namesi
Name:Rh3
Synonyms:RH92CD
ORF Names:CG10888
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0003249. Rh3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757ExtracellularAdd
BLAST
Transmembranei58 – 8225Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini83 – 9412CytoplasmicAdd
BLAST
Transmembranei95 – 11925Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini120 – 13314ExtracellularAdd
BLAST
Transmembranei134 – 15320Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini154 – 17118CytoplasmicAdd
BLAST
Transmembranei172 – 19625Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini197 – 22024ExtracellularAdd
BLAST
Transmembranei221 – 24828Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini249 – 28436CytoplasmicAdd
BLAST
Transmembranei285 – 30824Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini309 – 3168Extracellular
Transmembranei317 – 34125Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini342 – 38342CytoplasmicAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: FlyBase
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Opsin Rh3PRO_0000197627Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi13 – 131N-linked (GlcNAc...)Curated
Disulfide bondi130 ↔ 207PROSITE-ProRule annotation
Modified residuei328 – 3281N6-(retinylidene)lysine

Post-translational modificationi

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP04950.
PRIDEiP04950.

Expressioni

Gene expression databases

BgeeiP04950.
GenevisibleiP04950. DM.

Interactioni

Protein-protein interaction databases

BioGridi67383. 1 interaction.
STRINGi7227.FBpp0083284.

Structurei

3D structure databases

ProteinModelPortaliP04950.
SMRiP04950. Positions 46-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118977.
InParanoidiP04950.
KOiK04255.
OMAiDFMMMIK.
OrthoDBiEOG70PBXK.
PhylomeDBiP04950.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000856. Opsin_RH3/RH4.
IPR027430. Retinal_BS.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00577. OPSINRH3RH4.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P04950-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGNVSSSL FGNVSTALRP EARLSAETRL LGWNVPPEEL RHIPEHWLTY
60 70 80 90 100
PEPPESMNYL LGTLYIFFTL MSMLGNGLVI WVFSAAKSLR TPSNILVINL
110 120 130 140 150
AFCDFMMMVK TPIFIYNSFH QGYALGHLGC QIFGIIGSYT GIAAGATNAF
160 170 180 190 200
IAYDRFNVIT RPMEGKMTHG KAIAMIIFIY MYATPWVVAC YTETWGRFVP
210 220 230 240 250
EGYLTSCTFD YLTDNFDTRL FVACIFFFSF VCPTTMITYY YSQIVGHVFS
260 270 280 290 300
HEKALRDQAK KMNVESLRSN VDKNKETAEI RIAKAAITIC FLFFCSWTPY
310 320 330 340 350
GVMSLIGAFG DKTLLTPGAT MIPACACKMV ACIDPFVYAI SHPRYRMELQ
360 370 380
KRCPWLALNE KAPESSAVAS TSTTQEPQQT TAA
Length:383
Mass (Da):42,940
Last modified:August 13, 1987 - v1
Checksum:iBF9D009A25CA6343
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00043 Genomic DNA. Translation: CAA68259.1.
M17718 Genomic DNA. Translation: AAA28854.1.
AE014297 Genomic DNA. Translation: AAG22157.1.
AF184224 mRNA. Translation: AAD55735.1.
PIRiA26768.
RefSeqiNP_524411.1. NM_079687.3.
UniGeneiDm.2396.

Genome annotation databases

EnsemblMetazoaiFBtr0083876; FBpp0083284; FBgn0003249.
GeneIDi42398.
KEGGidme:Dmel_CG10888.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00043 Genomic DNA. Translation: CAA68259.1.
M17718 Genomic DNA. Translation: AAA28854.1.
AE014297 Genomic DNA. Translation: AAG22157.1.
AF184224 mRNA. Translation: AAD55735.1.
PIRiA26768.
RefSeqiNP_524411.1. NM_079687.3.
UniGeneiDm.2396.

3D structure databases

ProteinModelPortaliP04950.
SMRiP04950. Positions 46-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi67383. 1 interaction.
STRINGi7227.FBpp0083284.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP04950.
PRIDEiP04950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0083876; FBpp0083284; FBgn0003249.
GeneIDi42398.
KEGGidme:Dmel_CG10888.

Organism-specific databases

CTDi42398.
FlyBaseiFBgn0003249. Rh3.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118977.
InParanoidiP04950.
KOiK04255.
OMAiDFMMMIK.
OrthoDBiEOG70PBXK.
PhylomeDBiP04950.

Enzyme and pathway databases

ReactomeiR-DME-2187335. The retinoid cycle in cones (daylight vision).
R-DME-2453902. The canonical retinoid cycle in rods (twilight vision).
R-DME-2485179. Activation of the phototransduction cascade.
R-DME-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-DME-419771. Opsins.
R-DME-5620916. VxPx cargo-targeting to cilium.

Miscellaneous databases

ChiTaRSiRh3. fly.
GenomeRNAii42398.
PROiP04950.

Gene expression databases

BgeeiP04950.
GenevisibleiP04950. DM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000856. Opsin_RH3/RH4.
IPR027430. Retinal_BS.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00577. OPSINRH3RH4.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An opsin gene that is expressed only in the R7 photoreceptor cell of Drosophila."
    Fryxell K.J., Meyerowitz E.M.
    EMBO J. 6:443-451(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Canton-S.
  2. "A rhodopsin gene expressed in photoreceptor cell R7 of the Drosophila eye: homologies with other signal-transducing molecules."
    Zuker C.S., Montell C., Jones K., Laverty T., Rubin G.M.
    J. Neurosci. 7:1550-1557(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.
  6. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 34-355.

Entry informationi

Entry nameiOPS3_DROME
AccessioniPrimary (citable) accession number: P04950
Secondary accession number(s): Q9TX53
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 6, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

Each Drosophila eye is composed of 800 facets or ommatidia. Each ommatidium contains 8 photoreceptor cells (R1-R8), the R1 to R6 cells are outer cells, while R7 and R8 are inner cells.
Opsin Rh3 is sensitive to UV light.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.