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Protein

Band 3 anion transport protein

Gene

Slc4a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Anion exchange, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-MMU-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-MMU-425381. Bicarbonate transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Band 3 anion transport protein
Alternative name(s):
Anion exchange protein 1
Short name:
AE 1
Short name:
Anion exchanger 1
MEB3
Solute carrier family 4 member 1
CD_antigen: CD233
Gene namesi
Name:Slc4a1
Synonyms:Ae1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:109393. Slc4a1.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein By similarity
  • Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 422CytoplasmicBy similarityAdd BLAST422
Transmembranei423 – 446Helical; Name=1By similarityAdd BLAST24
Topological domaini447 – 454ExtracellularBy similarity8
Transmembranei455 – 475Helical; Name=2By similarityAdd BLAST21
Topological domaini476 – 478CytoplasmicBy similarity3
Transmembranei479 – 495Discontinuously helical; Name=3By similarityAdd BLAST17
Topological domaini496 – 504ExtracellularBy similarity9
Transmembranei505 – 525Helical; Name=4By similarityAdd BLAST21
Topological domaini526 – 537CytoplasmicBy similarityAdd BLAST12
Transmembranei538 – 560Helical; Name=5By similarityAdd BLAST23
Topological domaini561 – 588ExtracellularBy similarityAdd BLAST28
Transmembranei589 – 609Helical; Name=6By similarityAdd BLAST21
Topological domaini610 – 620CytoplasmicBy similarityAdd BLAST11
Transmembranei621 – 641Helical; Name=7By similarityAdd BLAST21
Topological domaini642 – 681ExtracellularBy similarityAdd BLAST40
Transmembranei682 – 702Helical; Name=8By similarityAdd BLAST21
Topological domaini703 – 718CytoplasmicBy similarityAdd BLAST16
Transmembranei719 – 737Helical; Name=9By similarityAdd BLAST19
Transmembranei738 – 755Discontinuously helical; Name=10By similarityAdd BLAST18
Topological domaini756 – 778CytoplasmicBy similarityAdd BLAST23
Transmembranei779 – 799Helical; Name=11By similarityAdd BLAST21
Transmembranei800 – 818Helical; Name=12By similarityAdd BLAST19
Topological domaini819 – 856CytoplasmicBy similarityAdd BLAST38
Intramembranei857 – 887Discontinuously helicalBy similarityAdd BLAST31
Topological domaini888 – 929CytoplasmicBy similarityAdd BLAST42

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB
  • blood microparticle Source: MGI
  • cortical cytoskeleton Source: MGI
  • extracellular exosome Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Gypa is not incorporated in the erythrocyte membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000792101 – 929Band 3 anion transport proteinAdd BLAST929

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei18PhosphoserineCombined sources1
Modified residuei31PhosphotyrosineBy similarity1
Modified residuei56PhosphotyrosineBy similarity1
Modified residuei199PhosphoserineBy similarity1
Modified residuei222PhosphoserineBy similarity1
Modified residuei363PhosphoserineCombined sources1
Modified residuei372PhosphotyrosineBy similarity1
Modified residuei374PhosphothreonineCombined sources1
Glycosylationi660N-linked (GlcNAc...)Curated1
Lipidationi861S-palmitoyl cysteineBy similarity1
Modified residuei922PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP04919.
PeptideAtlasiP04919.
PRIDEiP04919.

PTM databases

iPTMnetiP04919.
PhosphoSitePlusiP04919.

Expressioni

Tissue specificityi

Detected in erythrocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000006574.
ExpressionAtlasiP04919. baseline and differential.
GenevisibleiP04919. MM.

Interactioni

Subunit structurei

A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM. Isoform 2 interacts with TMEM139.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203312. 2 interactors.
IntActiP04919. 4 interactors.
MINTiMINT-1861446.
STRINGi10090.ENSMUSP00000006749.

Structurei

3D structure databases

ProteinModelPortaliP04919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 303GlobularBy similarityAdd BLAST235
Regioni190 – 199Interaction with ANK1By similarity10
Regioni317 – 370Dimerization armBy similarityAdd BLAST54

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP04919.
KOiK06573.
OMAiFKPPKYH.
OrthoDBiEOG091G01FT.
PhylomeDBiP04919.
TreeFamiTF313630.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04919-1) [UniParc]FASTAAdd to basket
Also known as: Erythrocyte

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMRDHEEV LEIPDRDSEE ELENIIGQIA YRDLTIPVTE MQDPEALPTE
60 70 80 90 100
QTATDYVPSS TSTPHPSSGQ VYVELQELMM DQRNQELQWV EAAHWIGLEE
110 120 130 140 150
NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLGLAET SLAGVANHLL
160 170 180 190 200
DCFIYEDQIR PQDREELLRA LLLKRSHAED LGNLEGVKPA VLTRSGGASE
210 220 230 240 250
PLLPHQPSLE TQLYCGQAEG GSEGPSTSGT LKIPPDSETT LVLVGRANFL
260 270 280 290 300
EKPVLGFVRL KEAVPLEDLV LPEPVGFLLV LLGPEAPHVD YTQLGRAAAT
310 320 330 340 350
LMTERVFRIT ASMAHNREEL LRSLESFLDC SLVLPPTDAP SEKALLNLVP
360 370 380 390 400
VQKELLRRRY LPSPAKPDPN LYNTLDLNGG KGGPGDEDDP LRRTGRIFGG
410 420 430 440 450
LIRDIRRRYP YYLSDITDAL SPQVLAAVIF IYFAALSPAV TFGGLLGEKT
460 470 480 490 500
RNLMGVSELL ISTAVQGILF ALLGAQPLLV LGFSGPLLVF EEAFFSFCES
510 520 530 540 550
NNLEYIVGRA WIGFWLILLV MLVVAFEGSF LVQYISRYTQ EIFSFLISLI
560 570 580 590 600
FIYETFSKLI KIFQDYPLQQ TYAPVVMKPK PQGPVPNTAL FSLVLMAGTF
610 620 630 640 650
LLAMTLRKFK NSTYFPGKLR RVIGDFGVPI SILIMVLVDS FIKGTYTQKL
660 670 680 690 700
SVPDGLKVSN SSARGWVIHP LGLYRLFPTW MMFASVLPAL LVFILIFLES
710 720 730 740 750
QITTLIVSKP ERKMIKGSGF HLDLLLVVGM GGVAALFGMP WLSATTVRSV
760 770 780 790 800
THANALTVMG KASGPGAAAQ IQEVKEQRIS GLLVSVLVGL SILMEPILSR
810 820 830 840 850
IPLAVLFGIF LYMGVTSLSG IQLFDRILLL FKPPKYHPDV PFVKRVKTWR
860 870 880 890 900
MHLFTGIQII CLAVLWVVKS TPASLALPFV LILTVPLRRL ILPLIFRELE
910 920
LQCLDGDDAK VTFDEENGLD EYDEVPMPV
Length:929
Mass (Da):103,136
Last modified:August 13, 1987 - v1
Checksum:i5C0E281C394FB614
GO
Isoform 2 (identifier: P04919-2) [UniParc]FASTAAdd to basket
Also known as: Kidney

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Show »
Length:850
Mass (Da):94,234
Checksum:i492427A73FAA811A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti467G → S in CAA27555 (PubMed:3015590).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0004541 – 79Missing in isoform 2. CuratedAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02677 mRNA. Translation: CAA26506.1.
M29379 mRNA. Translation: AAA37187.1.
J02756 Genomic DNA. Translation: AAA37278.1.
BC052419 mRNA. Translation: AAH52419.1.
BC053429 mRNA. Translation: AAH53429.1.
X03917 mRNA. Translation: CAA27555.1.
CCDSiCCDS25496.1. [P04919-1]
PIRiA25314.
RefSeqiNP_035533.1. NM_011403.2. [P04919-1]
UniGeneiMm.7248.

Genome annotation databases

EnsembliENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574. [P04919-1]
GeneIDi20533.
KEGGimmu:20533.
UCSCiuc007lrp.2. mouse. [P04919-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02677 mRNA. Translation: CAA26506.1.
M29379 mRNA. Translation: AAA37187.1.
J02756 Genomic DNA. Translation: AAA37278.1.
BC052419 mRNA. Translation: AAH52419.1.
BC053429 mRNA. Translation: AAH53429.1.
X03917 mRNA. Translation: CAA27555.1.
CCDSiCCDS25496.1. [P04919-1]
PIRiA25314.
RefSeqiNP_035533.1. NM_011403.2. [P04919-1]
UniGeneiMm.7248.

3D structure databases

ProteinModelPortaliP04919.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203312. 2 interactors.
IntActiP04919. 4 interactors.
MINTiMINT-1861446.
STRINGi10090.ENSMUSP00000006749.

PTM databases

iPTMnetiP04919.
PhosphoSitePlusiP04919.

Proteomic databases

PaxDbiP04919.
PeptideAtlasiP04919.
PRIDEiP04919.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574. [P04919-1]
GeneIDi20533.
KEGGimmu:20533.
UCSCiuc007lrp.2. mouse. [P04919-1]

Organism-specific databases

CTDi6521.
MGIiMGI:109393. Slc4a1.

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP04919.
KOiK06573.
OMAiFKPPKYH.
OrthoDBiEOG091G01FT.
PhylomeDBiP04919.
TreeFamiTF313630.

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-MMU-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-MMU-425381. Bicarbonate transporters.

Miscellaneous databases

ChiTaRSiSlc4a1. mouse.
PROiP04919.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006574.
ExpressionAtlasiP04919. baseline and differential.
GenevisibleiP04919. MM.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3AT_MOUSE
AccessioniPrimary (citable) accession number: P04919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: November 2, 2016
This is version 166 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.