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P04919 (B3AT_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 144. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Band 3 anion transport protein
Alternative name(s):
Anion exchange protein 1
Short name=AE 1
Short name=Anion exchanger 1
MEB3
Solute carrier family 4 member 1
CD_antigen=CD233
Gene names
Name:Slc4a1
Synonyms:Ae1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length929 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.

Subunit structure

A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Basolateral cell membrane; Multi-pass membrane protein By similarity. Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney By similarity. Ref.1

Tissue specificity

Erythrocytes.

Disruption phenotype

Gypa is not incorporated in the erythrocyte membrane. Ref.8

Sequence similarities

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]

Ontologies

Keywords
   Biological processAnion exchange
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMAcetylation
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processbicarbonate transport

Inferred from sequence or structural similarity. Source: UniProtKB

chloride transmembrane transport

Inferred from direct assay PubMed 17690150. Source: GOC

chloride transport

Inferred from direct assay PubMed 17690150. Source: UniProtKB

   Cellular_componentZ disc

Inferred from sequence or structural similarity. Source: UniProtKB

basolateral plasma membrane

Inferred from direct assay PubMed 12388412PubMed 16077082. Source: UniProtKB

cortical cytoskeleton

Inferred from direct assay PubMed 18723693. Source: MGI

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

membrane

Inferred from direct assay PubMed 17690150. Source: UniProtKB

   Molecular_functionanion:anion antiporter activity

Inferred from sequence or structural similarity. Source: UniProtKB

bicarbonate transmembrane transporter activity

Inferred from sequence or structural similarity. Source: UniProtKB

chloride transmembrane transporter activity

Inferred from direct assay PubMed 17690150. Source: UniProtKB

inorganic anion exchanger activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 11591653PubMed 9422766. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Erythrocyte (identifier: P04919-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Kidney (identifier: P04919-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 929929Band 3 anion transport protein
PRO_0000079210

Regions

Topological domain1 – 422422Cytoplasmic Potential
Transmembrane423 – 44321Helical; Potential
Transmembrane456 – 47520Helical; Potential
Transmembrane479 – 49820Helical; Potential
Transmembrane510 – 52920Helical; Potential
Transmembrane542 – 56019Helical; Potential
Topological domain561 – 58626Extracellular Potential
Transmembrane587 – 60620Helical; Potential
Topological domain607 – 62115Cytoplasmic Potential
Transmembrane622 – 64221Helical; Potential
Topological domain643 – 67836Extracellular Potential
Transmembrane679 – 69820Helical; Potential
Transmembrane717 – 73721Helical; Potential
Transmembrane781 – 79818Helical; Potential
Transmembrane803 – 82422Helical; Potential
Transmembrane862 – 88322Helical; Potential
Region69 – 303235Globular By similarity
Region190 – 19910Interaction with ANK1 By similarity
Region317 – 37054Dimerization arm By similarity
Region423 – 929507Membrane (anion exchange)

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue311Phosphotyrosine By similarity
Modified residue561Phosphotyrosine By similarity
Modified residue3721Phosphotyrosine By similarity
Modified residue9221Phosphotyrosine By similarity
Lipidation8611S-palmitoyl cysteine By similarity
Glycosylation6601N-linked (GlcNAc...) Probable

Natural variations

Alternative sequence1 – 7979Missing in isoform Kidney.
VSP_000454

Experimental info

Sequence conflict4671G → S in CAA27555. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform Erythrocyte [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 5C0E281C394FB614

FASTA929103,136
        10         20         30         40         50         60 
MGDMRDHEEV LEIPDRDSEE ELENIIGQIA YRDLTIPVTE MQDPEALPTE QTATDYVPSS 

        70         80         90        100        110        120 
TSTPHPSSGQ VYVELQELMM DQRNQELQWV EAAHWIGLEE NLREDGVWGR PHLSYLTFWS 

       130        140        150        160        170        180 
LLELQKVFSK GTFLLGLAET SLAGVANHLL DCFIYEDQIR PQDREELLRA LLLKRSHAED 

       190        200        210        220        230        240 
LGNLEGVKPA VLTRSGGASE PLLPHQPSLE TQLYCGQAEG GSEGPSTSGT LKIPPDSETT 

       250        260        270        280        290        300 
LVLVGRANFL EKPVLGFVRL KEAVPLEDLV LPEPVGFLLV LLGPEAPHVD YTQLGRAAAT 

       310        320        330        340        350        360 
LMTERVFRIT ASMAHNREEL LRSLESFLDC SLVLPPTDAP SEKALLNLVP VQKELLRRRY 

       370        380        390        400        410        420 
LPSPAKPDPN LYNTLDLNGG KGGPGDEDDP LRRTGRIFGG LIRDIRRRYP YYLSDITDAL 

       430        440        450        460        470        480 
SPQVLAAVIF IYFAALSPAV TFGGLLGEKT RNLMGVSELL ISTAVQGILF ALLGAQPLLV 

       490        500        510        520        530        540 
LGFSGPLLVF EEAFFSFCES NNLEYIVGRA WIGFWLILLV MLVVAFEGSF LVQYISRYTQ 

       550        560        570        580        590        600 
EIFSFLISLI FIYETFSKLI KIFQDYPLQQ TYAPVVMKPK PQGPVPNTAL FSLVLMAGTF 

       610        620        630        640        650        660 
LLAMTLRKFK NSTYFPGKLR RVIGDFGVPI SILIMVLVDS FIKGTYTQKL SVPDGLKVSN 

       670        680        690        700        710        720 
SSARGWVIHP LGLYRLFPTW MMFASVLPAL LVFILIFLES QITTLIVSKP ERKMIKGSGF 

       730        740        750        760        770        780 
HLDLLLVVGM GGVAALFGMP WLSATTVRSV THANALTVMG KASGPGAAAQ IQEVKEQRIS 

       790        800        810        820        830        840 
GLLVSVLVGL SILMEPILSR IPLAVLFGIF LYMGVTSLSG IQLFDRILLL FKPPKYHPDV 

       850        860        870        880        890        900 
PFVKRVKTWR MHLFTGIQII CLAVLWVVKS TPASLALPFV LILTVPLRRL ILPLIFRELE 

       910        920 
LQCLDGDDAK VTFDEENGLD EYDEVPMPV 

« Hide

Isoform Kidney [UniParc].

Checksum: 492427A73FAA811A
Show »

FASTA85094,234

References

« Hide 'large scale' references
[1]"Primary structure and transmembrane orientation of the murine anion exchange protein."
Kopito R.R., Lodish H.F.
Nature 316:234-238(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
[2]"Structure of the murine anion exchange protein."
Kopito R.R., Lodish H.F.
J. Cell. Biochem. 29:1-17(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structure and organization of the murine band 3 gene."
Kopito R.R., Andersson M., Lodish H.F.
J. Biol. Chem. 262:8035-8040(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]Kopito R.R.
Submitted (JUL-1987) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ERYTHROCYTE).
Strain: C57BL/6.
Tissue: Brain and Limb.
[6]"Cloning and structural characterization of a human non-erythroid band 3-like protein."
Demuth D.R., Showe L.C., Ballantine M., Palumbo A., Fraser P.J., Cioe L., Rovera G., Curtis P.J.
EMBO J. 5:1205-1214(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-929.
[7]"Major proteolytic fragments of the murine band 3 protein as obtained after in situ proteolysis."
Raida M., Wendel J., Kojro E., Fahrenholz F., Fasold H., Legrum B., Passow H.
Biochim. Biophys. Acta 980:291-298(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 33-47; 360-375; 382-395 AND 578-590.
[8]"Complete deficiency of glycophorin A in red blood cells from mice with targeted inactivation of the band 3 (AE1) gene."
Hassoun H., Hanada T., Lutchman M., Sahr K.E., Palek J., Hanspal M., Chishti A.H.
Blood 91:2146-2151(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[9]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[10]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X02677 mRNA. Translation: CAA26506.1.
M29379 mRNA. Translation: AAA37187.1.
J02756 Genomic DNA. Translation: AAA37278.1.
BC052419 mRNA. Translation: AAH52419.1.
BC053429 mRNA. Translation: AAH53429.1.
X03917 mRNA. Translation: CAA27555.1.
CCDSCCDS25496.1. [P04919-1]
PIRA25314.
RefSeqNP_035533.1. NM_011403.2. [P04919-1]
UniGeneMm.7248.

3D structure databases

ProteinModelPortalP04919.
SMRP04919. Positions 70-368, 408-449, 821-853.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203312. 2 interactions.
IntActP04919. 4 interactions.
MINTMINT-1861446.

PTM databases

PhosphoSiteP04919.

Proteomic databases

MaxQBP04919.
PaxDbP04919.
PRIDEP04919.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574. [P04919-1]
GeneID20533.
KEGGmmu:20533.
UCSCuc007lrp.2. mouse. [P04919-1]

Organism-specific databases

CTD6521.
MGIMGI:109393. Slc4a1.

Phylogenomic databases

eggNOGNOG268067.
HOGENOMHOG000280683.
HOVERGENHBG004326.
InParanoidP04919.
KOK06573.
OMAEYDEVAM.
OrthoDBEOG7TMZR0.
PhylomeDBP04919.
TreeFamTF313630.

Gene expression databases

ArrayExpressP04919.
BgeeP04919.
GenevestigatorP04919.

Family and domain databases

Gene3D3.40.1100.10. 1 hit.
InterProIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERPTHR11453. PTHR11453. 1 hit.
PfamPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMSSF55804. SSF55804. 1 hit.
TIGRFAMsTIGR00834. ae. 1 hit.
PROSITEPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC4A1. mouse.
NextBio298795.
PROP04919.
SOURCESearch...

Entry information

Entry nameB3AT_MOUSE
AccessionPrimary (citable) accession number: P04919
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot