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P04919

- B3AT_MOUSE

UniProt

P04919 - B3AT_MOUSE

Protein

Band 3 anion transport protein

Gene

Slc4a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.

    GO - Molecular functioni

    1. anion:anion antiporter activity Source: UniProtKB
    2. bicarbonate transmembrane transporter activity Source: UniProtKB
    3. chloride transmembrane transporter activity Source: UniProtKB
    4. inorganic anion exchanger activity Source: UniProtKB
    5. protein binding Source: MGI

    GO - Biological processi

    1. bicarbonate transport Source: UniProtKB
    2. chloride transmembrane transport Source: GOC
    3. chloride transport Source: UniProtKB

    Keywords - Biological processi

    Anion exchange, Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199094. Erythrocytes take up carbon dioxide and release oxygen.
    REACT_199098. Erythrocytes take up oxygen and release carbon dioxide.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Band 3 anion transport protein
    Alternative name(s):
    Anion exchange protein 1
    Short name:
    AE 1
    Short name:
    Anion exchanger 1
    MEB3
    Solute carrier family 4 member 1
    CD_antigen: CD233
    Gene namesi
    Name:Slc4a1
    Synonyms:Ae1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:109393. Slc4a1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB
    2. cortical cytoskeleton Source: MGI
    3. integral component of plasma membrane Source: UniProtKB
    4. membrane Source: UniProtKB
    5. Z disc Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Gypa is not incorporated in the erythrocyte membrane.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 929929Band 3 anion transport proteinPRO_0000079210Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei31 – 311PhosphotyrosineBy similarity
    Modified residuei56 – 561PhosphotyrosineBy similarity
    Modified residuei372 – 3721PhosphotyrosineBy similarity
    Glycosylationi660 – 6601N-linked (GlcNAc...)Curated
    Lipidationi861 – 8611S-palmitoyl cysteineBy similarity
    Modified residuei922 – 9221PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP04919.
    PaxDbiP04919.
    PRIDEiP04919.

    PTM databases

    PhosphoSiteiP04919.

    Expressioni

    Tissue specificityi

    Erythrocytes.

    Gene expression databases

    ArrayExpressiP04919.
    BgeeiP04919.
    GenevestigatoriP04919.

    Interactioni

    Subunit structurei

    A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM By similarity.By similarity

    Protein-protein interaction databases

    BioGridi203312. 2 interactions.
    IntActiP04919. 4 interactions.
    MINTiMINT-1861446.

    Structurei

    3D structure databases

    ProteinModelPortaliP04919.
    SMRiP04919. Positions 70-368, 408-449, 821-853.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 422422CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini561 – 58626ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini607 – 62115CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini643 – 67836ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei423 – 44321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei456 – 47520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei479 – 49820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei510 – 52920HelicalSequence AnalysisAdd
    BLAST
    Transmembranei542 – 56019HelicalSequence AnalysisAdd
    BLAST
    Transmembranei587 – 60620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei622 – 64221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei679 – 69820HelicalSequence AnalysisAdd
    BLAST
    Transmembranei717 – 73721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei781 – 79818HelicalSequence AnalysisAdd
    BLAST
    Transmembranei803 – 82422HelicalSequence AnalysisAdd
    BLAST
    Transmembranei862 – 88322HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 303235GlobularBy similarityAdd
    BLAST
    Regioni190 – 19910Interaction with ANK1By similarity
    Regioni317 – 37054Dimerization armBy similarityAdd
    BLAST
    Regioni423 – 929507Membrane (anion exchange)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268067.
    HOGENOMiHOG000280683.
    HOVERGENiHBG004326.
    InParanoidiP04919.
    KOiK06573.
    OMAiEYDEVAM.
    OrthoDBiEOG7TMZR0.
    PhylomeDBiP04919.
    TreeFamiTF313630.

    Family and domain databases

    Gene3Di3.40.1100.10. 1 hit.
    InterProiIPR001717. Anion_exchange.
    IPR002977. Anion_exchange_1.
    IPR018241. Anion_exchange_CS.
    IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view]
    PANTHERiPTHR11453. PTHR11453. 1 hit.
    PfamiPF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 2 hits.
    [Graphical view]
    PRINTSiPR00165. ANIONEXCHNGR.
    PR01187. ANIONEXHNGR1.
    PR01231. HCO3TRNSPORT.
    SUPFAMiSSF55804. SSF55804. 1 hit.
    TIGRFAMsiTIGR00834. ae. 1 hit.
    PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
    PS00220. ANION_EXCHANGER_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Erythrocyte (identifier: P04919-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGDMRDHEEV LEIPDRDSEE ELENIIGQIA YRDLTIPVTE MQDPEALPTE    50
    QTATDYVPSS TSTPHPSSGQ VYVELQELMM DQRNQELQWV EAAHWIGLEE 100
    NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLGLAET SLAGVANHLL 150
    DCFIYEDQIR PQDREELLRA LLLKRSHAED LGNLEGVKPA VLTRSGGASE 200
    PLLPHQPSLE TQLYCGQAEG GSEGPSTSGT LKIPPDSETT LVLVGRANFL 250
    EKPVLGFVRL KEAVPLEDLV LPEPVGFLLV LLGPEAPHVD YTQLGRAAAT 300
    LMTERVFRIT ASMAHNREEL LRSLESFLDC SLVLPPTDAP SEKALLNLVP 350
    VQKELLRRRY LPSPAKPDPN LYNTLDLNGG KGGPGDEDDP LRRTGRIFGG 400
    LIRDIRRRYP YYLSDITDAL SPQVLAAVIF IYFAALSPAV TFGGLLGEKT 450
    RNLMGVSELL ISTAVQGILF ALLGAQPLLV LGFSGPLLVF EEAFFSFCES 500
    NNLEYIVGRA WIGFWLILLV MLVVAFEGSF LVQYISRYTQ EIFSFLISLI 550
    FIYETFSKLI KIFQDYPLQQ TYAPVVMKPK PQGPVPNTAL FSLVLMAGTF 600
    LLAMTLRKFK NSTYFPGKLR RVIGDFGVPI SILIMVLVDS FIKGTYTQKL 650
    SVPDGLKVSN SSARGWVIHP LGLYRLFPTW MMFASVLPAL LVFILIFLES 700
    QITTLIVSKP ERKMIKGSGF HLDLLLVVGM GGVAALFGMP WLSATTVRSV 750
    THANALTVMG KASGPGAAAQ IQEVKEQRIS GLLVSVLVGL SILMEPILSR 800
    IPLAVLFGIF LYMGVTSLSG IQLFDRILLL FKPPKYHPDV PFVKRVKTWR 850
    MHLFTGIQII CLAVLWVVKS TPASLALPFV LILTVPLRRL ILPLIFRELE 900
    LQCLDGDDAK VTFDEENGLD EYDEVPMPV 929
    Length:929
    Mass (Da):103,136
    Last modified:August 13, 1987 - v1
    Checksum:i5C0E281C394FB614
    GO
    Isoform Kidney (identifier: P04919-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-79: Missing.

    Show »
    Length:850
    Mass (Da):94,234
    Checksum:i492427A73FAA811A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti467 – 4671G → S in CAA27555. (PubMed:3015590)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7979Missing in isoform Kidney. CuratedVSP_000454Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02677 mRNA. Translation: CAA26506.1.
    M29379 mRNA. Translation: AAA37187.1.
    J02756 Genomic DNA. Translation: AAA37278.1.
    BC052419 mRNA. Translation: AAH52419.1.
    BC053429 mRNA. Translation: AAH53429.1.
    X03917 mRNA. Translation: CAA27555.1.
    CCDSiCCDS25496.1. [P04919-1]
    PIRiA25314.
    RefSeqiNP_035533.1. NM_011403.2. [P04919-1]
    UniGeneiMm.7248.

    Genome annotation databases

    EnsembliENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574. [P04919-1]
    GeneIDi20533.
    KEGGimmu:20533.
    UCSCiuc007lrp.2. mouse. [P04919-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02677 mRNA. Translation: CAA26506.1 .
    M29379 mRNA. Translation: AAA37187.1 .
    J02756 Genomic DNA. Translation: AAA37278.1 .
    BC052419 mRNA. Translation: AAH52419.1 .
    BC053429 mRNA. Translation: AAH53429.1 .
    X03917 mRNA. Translation: CAA27555.1 .
    CCDSi CCDS25496.1. [P04919-1 ]
    PIRi A25314.
    RefSeqi NP_035533.1. NM_011403.2. [P04919-1 ]
    UniGenei Mm.7248.

    3D structure databases

    ProteinModelPortali P04919.
    SMRi P04919. Positions 70-368, 408-449, 821-853.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203312. 2 interactions.
    IntActi P04919. 4 interactions.
    MINTi MINT-1861446.

    PTM databases

    PhosphoSitei P04919.

    Proteomic databases

    MaxQBi P04919.
    PaxDbi P04919.
    PRIDEi P04919.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006749 ; ENSMUSP00000006749 ; ENSMUSG00000006574 . [P04919-1 ]
    GeneIDi 20533.
    KEGGi mmu:20533.
    UCSCi uc007lrp.2. mouse. [P04919-1 ]

    Organism-specific databases

    CTDi 6521.
    MGIi MGI:109393. Slc4a1.

    Phylogenomic databases

    eggNOGi NOG268067.
    HOGENOMi HOG000280683.
    HOVERGENi HBG004326.
    InParanoidi P04919.
    KOi K06573.
    OMAi EYDEVAM.
    OrthoDBi EOG7TMZR0.
    PhylomeDBi P04919.
    TreeFami TF313630.

    Enzyme and pathway databases

    Reactomei REACT_199094. Erythrocytes take up carbon dioxide and release oxygen.
    REACT_199098. Erythrocytes take up oxygen and release carbon dioxide.

    Miscellaneous databases

    ChiTaRSi SLC4A1. mouse.
    NextBioi 298795.
    PROi P04919.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P04919.
    Bgeei P04919.
    Genevestigatori P04919.

    Family and domain databases

    Gene3Di 3.40.1100.10. 1 hit.
    InterProi IPR001717. Anion_exchange.
    IPR002977. Anion_exchange_1.
    IPR018241. Anion_exchange_CS.
    IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view ]
    PANTHERi PTHR11453. PTHR11453. 1 hit.
    Pfami PF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 2 hits.
    [Graphical view ]
    PRINTSi PR00165. ANIONEXCHNGR.
    PR01187. ANIONEXHNGR1.
    PR01231. HCO3TRNSPORT.
    SUPFAMi SSF55804. SSF55804. 1 hit.
    TIGRFAMsi TIGR00834. ae. 1 hit.
    PROSITEi PS00219. ANION_EXCHANGER_1. 1 hit.
    PS00220. ANION_EXCHANGER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure and transmembrane orientation of the murine anion exchange protein."
      Kopito R.R., Lodish H.F.
      Nature 316:234-238(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    2. "Structure of the murine anion exchange protein."
      Kopito R.R., Lodish H.F.
      J. Cell. Biochem. 29:1-17(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Structure and organization of the murine band 3 gene."
      Kopito R.R., Andersson M., Lodish H.F.
      J. Biol. Chem. 262:8035-8040(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Kopito R.R.
      Submitted (JUL-1987) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ERYTHROCYTE).
      Strain: C57BL/6.
      Tissue: Brain and Limb.
    6. "Cloning and structural characterization of a human non-erythroid band 3-like protein."
      Demuth D.R., Showe L.C., Ballantine M., Palumbo A., Fraser P.J., Cioe L., Rovera G., Curtis P.J.
      EMBO J. 5:1205-1214(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-929.
    7. "Major proteolytic fragments of the murine band 3 protein as obtained after in situ proteolysis."
      Raida M., Wendel J., Kojro E., Fahrenholz F., Fasold H., Legrum B., Passow H.
      Biochim. Biophys. Acta 980:291-298(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 33-47; 360-375; 382-395 AND 578-590.
    8. "Complete deficiency of glycophorin A in red blood cells from mice with targeted inactivation of the band 3 (AE1) gene."
      Hassoun H., Hanada T., Lutchman M., Sahr K.E., Palek J., Hanspal M., Chishti A.H.
      Blood 91:2146-2151(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    9. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiB3AT_MOUSE
    AccessioniPrimary (citable) accession number: P04919
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3