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Protein

Band 3 anion transport protein

Gene

Slc4a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Anion exchange, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-MMU-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-MMU-425381. Bicarbonate transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Band 3 anion transport protein
Alternative name(s):
Anion exchange protein 1
Short name:
AE 1
Short name:
Anion exchanger 1
MEB3
Solute carrier family 4 member 1
CD_antigen: CD233
Gene namesi
Name:Slc4a1
Synonyms:Ae1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:109393. Slc4a1.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein By similarity
  • Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 422422CytoplasmicBy similarityAdd
BLAST
Transmembranei423 – 44624Helical; Name=1By similarityAdd
BLAST
Topological domaini447 – 4548ExtracellularBy similarity
Transmembranei455 – 47521Helical; Name=2By similarityAdd
BLAST
Topological domaini476 – 4783CytoplasmicBy similarity
Transmembranei479 – 49517Discontinuously helical; Name=3By similarityAdd
BLAST
Topological domaini496 – 5049ExtracellularBy similarity
Transmembranei505 – 52521Helical; Name=4By similarityAdd
BLAST
Topological domaini526 – 53712CytoplasmicBy similarityAdd
BLAST
Transmembranei538 – 56023Helical; Name=5By similarityAdd
BLAST
Topological domaini561 – 58828ExtracellularBy similarityAdd
BLAST
Transmembranei589 – 60921Helical; Name=6By similarityAdd
BLAST
Topological domaini610 – 62011CytoplasmicBy similarityAdd
BLAST
Transmembranei621 – 64121Helical; Name=7By similarityAdd
BLAST
Topological domaini642 – 68140ExtracellularBy similarityAdd
BLAST
Transmembranei682 – 70221Helical; Name=8By similarityAdd
BLAST
Topological domaini703 – 71816CytoplasmicBy similarityAdd
BLAST
Transmembranei719 – 73719Helical; Name=9By similarityAdd
BLAST
Transmembranei738 – 75518Discontinuously helical; Name=10By similarityAdd
BLAST
Topological domaini756 – 77823CytoplasmicBy similarityAdd
BLAST
Transmembranei779 – 79921Helical; Name=11By similarityAdd
BLAST
Transmembranei800 – 81819Helical; Name=12By similarityAdd
BLAST
Topological domaini819 – 85638CytoplasmicBy similarityAdd
BLAST
Intramembranei857 – 88731Discontinuously helicalBy similarityAdd
BLAST
Topological domaini888 – 92942CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB
  • blood microparticle Source: MGI
  • cortical cytoskeleton Source: MGI
  • extracellular exosome Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • membrane Source: UniProtKB
  • Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Gypa is not incorporated in the erythrocyte membrane.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Band 3 anion transport proteinPRO_0000079210Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei18 – 181PhosphoserineCombined sources
Modified residuei31 – 311PhosphotyrosineBy similarity
Modified residuei56 – 561PhosphotyrosineBy similarity
Modified residuei199 – 1991PhosphoserineBy similarity
Modified residuei222 – 2221PhosphoserineBy similarity
Modified residuei363 – 3631PhosphoserineCombined sources
Modified residuei372 – 3721PhosphotyrosineBy similarity
Modified residuei374 – 3741PhosphothreonineCombined sources
Glycosylationi660 – 6601N-linked (GlcNAc...)Curated
Lipidationi861 – 8611S-palmitoyl cysteineBy similarity
Modified residuei922 – 9221PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiP04919.
PaxDbiP04919.
PeptideAtlasiP04919.
PRIDEiP04919.

PTM databases

iPTMnetiP04919.
PhosphoSiteiP04919.

Expressioni

Tissue specificityi

Detected in erythrocytes (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000006574.
ExpressionAtlasiP04919. baseline and differential.
GenevisibleiP04919. MM.

Interactioni

Subunit structurei

A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM. Isoform 2 interacts with TMEM139.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203312. 2 interactions.
IntActiP04919. 4 interactions.
MINTiMINT-1861446.
STRINGi10090.ENSMUSP00000006749.

Structurei

3D structure databases

ProteinModelPortaliP04919.
SMRiP04919. Positions 70-368, 408-449, 821-853.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 303235GlobularBy similarityAdd
BLAST
Regioni190 – 19910Interaction with ANK1By similarity
Regioni317 – 37054Dimerization armBy similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP04919.
KOiK06573.
OMAiFKPPKYH.
OrthoDBiEOG091G01FT.
PhylomeDBiP04919.
TreeFamiTF313630.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04919-1) [UniParc]FASTAAdd to basket
Also known as: Erythrocyte

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMRDHEEV LEIPDRDSEE ELENIIGQIA YRDLTIPVTE MQDPEALPTE
60 70 80 90 100
QTATDYVPSS TSTPHPSSGQ VYVELQELMM DQRNQELQWV EAAHWIGLEE
110 120 130 140 150
NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLGLAET SLAGVANHLL
160 170 180 190 200
DCFIYEDQIR PQDREELLRA LLLKRSHAED LGNLEGVKPA VLTRSGGASE
210 220 230 240 250
PLLPHQPSLE TQLYCGQAEG GSEGPSTSGT LKIPPDSETT LVLVGRANFL
260 270 280 290 300
EKPVLGFVRL KEAVPLEDLV LPEPVGFLLV LLGPEAPHVD YTQLGRAAAT
310 320 330 340 350
LMTERVFRIT ASMAHNREEL LRSLESFLDC SLVLPPTDAP SEKALLNLVP
360 370 380 390 400
VQKELLRRRY LPSPAKPDPN LYNTLDLNGG KGGPGDEDDP LRRTGRIFGG
410 420 430 440 450
LIRDIRRRYP YYLSDITDAL SPQVLAAVIF IYFAALSPAV TFGGLLGEKT
460 470 480 490 500
RNLMGVSELL ISTAVQGILF ALLGAQPLLV LGFSGPLLVF EEAFFSFCES
510 520 530 540 550
NNLEYIVGRA WIGFWLILLV MLVVAFEGSF LVQYISRYTQ EIFSFLISLI
560 570 580 590 600
FIYETFSKLI KIFQDYPLQQ TYAPVVMKPK PQGPVPNTAL FSLVLMAGTF
610 620 630 640 650
LLAMTLRKFK NSTYFPGKLR RVIGDFGVPI SILIMVLVDS FIKGTYTQKL
660 670 680 690 700
SVPDGLKVSN SSARGWVIHP LGLYRLFPTW MMFASVLPAL LVFILIFLES
710 720 730 740 750
QITTLIVSKP ERKMIKGSGF HLDLLLVVGM GGVAALFGMP WLSATTVRSV
760 770 780 790 800
THANALTVMG KASGPGAAAQ IQEVKEQRIS GLLVSVLVGL SILMEPILSR
810 820 830 840 850
IPLAVLFGIF LYMGVTSLSG IQLFDRILLL FKPPKYHPDV PFVKRVKTWR
860 870 880 890 900
MHLFTGIQII CLAVLWVVKS TPASLALPFV LILTVPLRRL ILPLIFRELE
910 920
LQCLDGDDAK VTFDEENGLD EYDEVPMPV
Length:929
Mass (Da):103,136
Last modified:August 13, 1987 - v1
Checksum:i5C0E281C394FB614
GO
Isoform 2 (identifier: P04919-2) [UniParc]FASTAAdd to basket
Also known as: Kidney

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Show »
Length:850
Mass (Da):94,234
Checksum:i492427A73FAA811A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti467 – 4671G → S in CAA27555 (PubMed:3015590).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7979Missing in isoform 2. CuratedVSP_000454Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02677 mRNA. Translation: CAA26506.1.
M29379 mRNA. Translation: AAA37187.1.
J02756 Genomic DNA. Translation: AAA37278.1.
BC052419 mRNA. Translation: AAH52419.1.
BC053429 mRNA. Translation: AAH53429.1.
X03917 mRNA. Translation: CAA27555.1.
CCDSiCCDS25496.1. [P04919-1]
PIRiA25314.
RefSeqiNP_035533.1. NM_011403.2. [P04919-1]
UniGeneiMm.7248.

Genome annotation databases

EnsembliENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574. [P04919-1]
GeneIDi20533.
KEGGimmu:20533.
UCSCiuc007lrp.2. mouse. [P04919-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02677 mRNA. Translation: CAA26506.1.
M29379 mRNA. Translation: AAA37187.1.
J02756 Genomic DNA. Translation: AAA37278.1.
BC052419 mRNA. Translation: AAH52419.1.
BC053429 mRNA. Translation: AAH53429.1.
X03917 mRNA. Translation: CAA27555.1.
CCDSiCCDS25496.1. [P04919-1]
PIRiA25314.
RefSeqiNP_035533.1. NM_011403.2. [P04919-1]
UniGeneiMm.7248.

3D structure databases

ProteinModelPortaliP04919.
SMRiP04919. Positions 70-368, 408-449, 821-853.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203312. 2 interactions.
IntActiP04919. 4 interactions.
MINTiMINT-1861446.
STRINGi10090.ENSMUSP00000006749.

PTM databases

iPTMnetiP04919.
PhosphoSiteiP04919.

Proteomic databases

MaxQBiP04919.
PaxDbiP04919.
PeptideAtlasiP04919.
PRIDEiP04919.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006749; ENSMUSP00000006749; ENSMUSG00000006574. [P04919-1]
GeneIDi20533.
KEGGimmu:20533.
UCSCiuc007lrp.2. mouse. [P04919-1]

Organism-specific databases

CTDi6521.
MGIiMGI:109393. Slc4a1.

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
GeneTreeiENSGT00760000119021.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP04919.
KOiK06573.
OMAiFKPPKYH.
OrthoDBiEOG091G01FT.
PhylomeDBiP04919.
TreeFamiTF313630.

Enzyme and pathway databases

ReactomeiR-MMU-1237044. Erythrocytes take up carbon dioxide and release oxygen.
R-MMU-1247673. Erythrocytes take up oxygen and release carbon dioxide.
R-MMU-425381. Bicarbonate transporters.

Miscellaneous databases

ChiTaRSiSlc4a1. mouse.
PROiP04919.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000006574.
ExpressionAtlasiP04919. baseline and differential.
GenevisibleiP04919. MM.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3AT_MOUSE
AccessioniPrimary (citable) accession number: P04919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: September 7, 2016
This is version 164 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.