P04907 (GSTF3_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase 3
EC=2.5.1.18

Alternative name(s):
GST class-phi member 3
GST-III

Gene names

Name:

GST3

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Involved in the detoxification of certain herbicides.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the GST superfamily. Phi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 222	221	Glutathione S-transferase 3		PRO_0000185842
Regions
	Domain	2 – 83	82	GST N-terminal
	Domain	89 – 219	131	GST C-terminal
	Region	41 – 42	2	Glutathione binding By similarity
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	12	1	Glutathione By similarity
Experimental info
	Sequence conflict	108	1	H → Y Ref.2
	Sequence conflict	111 – 127	17	ASPLV…LLGGA → RVAAGVPAAREAAPGRR in CAA27957. Ref.2
	Sequence conflict	111 – 127	17	ASPLV…LLGGA → RVAAGVPAAREAAPGRR in CAA28053. Ref.2
	Sequence conflict	134	1	E → D in CAA27957. Ref.2
	Sequence conflict	134	1	E → D in CAA28053. Ref.2
	Sequence conflict	149 – 180	32	AHLAR…TSARP → RTSPATSTSPGTSSRSPTPT TRSYLLYLSKT in CAA27957. Ref.2
	Sequence conflict	184 – 189	6	GCVAAR → ARRRP in CAA27957. Ref.2
	Sequence conflict	200	1	A → V in CAA27957. Ref.2
	Sequence conflict	200	1	A → V in CAA28053. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P04907 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 4CB77A3B1B6E3C46	FASTA	222	23,849
	10 20 30 40 50 60 MAPLKLYGMP LSPNVVRVAT VLNEKGLDFE IVPVDLTTGA HKQPDFLALN PFGQIPALVD 70 80 90 100 110 120 GDEVLFESRA INRYIASKYA SEGTDLLPAT ASAAKLEVWL EVESHHFHPN ASPLVFQLLV 130 140 150 160 170 180 RPLLGGAPDA AVVEKHAEQL AKVLDVYEAH LARNKYLAGD EFTLADANHA LLPALTSARP 190 200 210 220 PRPGCVAARP HVKAWWEAIA ARPAFQKTVA AIPLPPPPSS SA

References

[1]	"Characterization and heterospecific expression of cDNA clones of genes in the maize GSH S-transferase multigene family." Grove G., Zarlengo R.P., Timmerman K.P., Li N.-Q., Tam M.F., Tu C.-P.D. Nucleic Acids Res. 16:425-438(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning and expression of a cDNA encoding a maize glutathione-S-transferase in E. coli." Moore R.E., Davies M.S., O'Connell K.M., Harding E.I., Wiegand R.C., Tiemeier D.C. Nucleic Acids Res. 14:7227-7235(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The maize two dimensional gel protein database: towards an integrated genome analysis program." Touzet P., Riccardi F., Morin C., Damerval C., Huet J.-C., Pernollet J.-C., Zivy M., de Vienne D. Theor. Appl. Genet. 93:997-1005(1996) [AGRICOLA] [Europe PMC] Cited for: PROTEIN SEQUENCE OF 143-153. Tissue: Coleoptile.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06755 mRNA. Translation: CAA29929.1. X04375 mRNA. Translation: CAA27957.1. X04455 mRNA. Translation: CAA28053.1.
PIR	XUZM31. A24703. XUZM32. S00717.
RefSeq	NP_001106075.1. NM_001112605.1.
UniGene	Zm.103382.
3D structure databases
ProteinModelPortal	P04907.
ModBase	Search...
Proteomic databases
PRIDE	P04907.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100127012.
KEGG	zma:100127012.
Organism-specific databases
Gramene	P04907.
MaizeGDB	65344.
Phylogenomic databases
HOGENOM	HOG000125746.
KO	K00799.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTF3_MAIZE

Accession

Primary (citable) accession number: P04907
Secondary accession number(s): P15542

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 86 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program