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P04906

- GSTP1_RAT

UniProt

P04906 - GSTP1_RAT

Protein

Glutathione S-transferase P

Gene

Gstp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration By similarity.By similarity

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81GlutathioneBy similarity
    Binding sitei14 – 141GlutathioneBy similarity
    Binding sitei39 – 391GlutathioneBy similarity
    Binding sitei45 – 451GlutathioneBy similarity

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. glutathione binding Source: RGD
    3. glutathione transferase activity Source: RGD
    4. JUN kinase binding Source: BHF-UCL
    5. kinase regulator activity Source: BHF-UCL

    GO - Biological processi

    1. cellular response to cell-matrix adhesion Source: RGD
    2. cellular response to epidermal growth factor stimulus Source: RGD
    3. cellular response to glucocorticoid stimulus Source: RGD
    4. cellular response to insulin stimulus Source: RGD
    5. cellular response to lipopolysaccharide Source: BHF-UCL
    6. common myeloid progenitor cell proliferation Source: BHF-UCL
    7. negative regulation of biosynthetic process Source: BHF-UCL
    8. negative regulation of ERK1 and ERK2 cascade Source: BHF-UCL
    9. negative regulation of fibroblast proliferation Source: BHF-UCL
    10. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: BHF-UCL
    11. negative regulation of interleukin-1 beta production Source: BHF-UCL
    12. negative regulation of JUN kinase activity Source: BHF-UCL
    13. negative regulation of leukocyte proliferation Source: BHF-UCL
    14. negative regulation of monocyte chemotactic protein-1 production Source: BHF-UCL
    15. negative regulation of nitric-oxide synthase biosynthetic process Source: BHF-UCL
    16. negative regulation of stress-activated MAPK cascade Source: BHF-UCL
    17. negative regulation of tumor necrosis factor production Source: BHF-UCL
    18. oligodendrocyte development Source: RGD
    19. organ regeneration Source: RGD
    20. positive regulation of superoxide anion generation Source: BHF-UCL
    21. regulation of ERK1 and ERK2 cascade Source: BHF-UCL
    22. regulation of stress-activated MAPK cascade Source: BHF-UCL
    23. response to amino acid Source: RGD
    24. response to estradiol Source: RGD
    25. response to ethanol Source: RGD
    26. response to L-ascorbic acid Source: RGD
    27. response to mercury ion Source: RGD
    28. response to nutrient levels Source: RGD
    29. response to reactive oxygen species Source: BHF-UCL
    30. response to toxic substance Source: RGD
    31. xenobiotic metabolic process Source: RGD

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase P (EC:2.5.1.18)
    Alternative name(s):
    Chain 7
    GST 7-7
    GST class-pi
    Gene namesi
    Name:Gstp1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi2758. Gstp1.

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity. Nucleus By similarity
    Note: The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RGD
    2. cytosol Source: RGD
    3. intracellular Source: BHF-UCL
    4. mitochondrion Source: UniProtKB-SubCell
    5. nucleus Source: RGD
    6. plasma membrane Source: RGD
    7. protein complex Source: BHF-UCL

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed3 Publications
    Chaini2 – 210209Glutathione S-transferase PPRO_0000185907Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei4 – 41Phosphotyrosine; by EGFRBy similarity
    Modified residuei103 – 1031N6-succinyllysineBy similarity
    Modified residuei116 – 1161N6-succinyllysineBy similarity
    Modified residuei128 – 1281N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP04906.
    PRIDEiP04906.

    2D gel databases

    World-2DPAGE0004:P04906.

    PTM databases

    PhosphoSiteiP04906.

    Miscellaneous databases

    PMAP-CutDBP04906.

    Expressioni

    Tissue specificityi

    Present in kidney, lung, testis and placenta, very low levels in liver.

    Gene expression databases

    ArrayExpressiP04906.
    GenevestigatoriP04906.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CDK5 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi246591. 1 interaction.
    IntActiP04906. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP04906.
    SMRiP04906. Positions 2-210.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8180GST N-terminalAdd
    BLAST
    Domaini83 – 204122GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni52 – 532Glutathione bindingBy similarity
    Regioni65 – 662Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Pi family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG05174.
    GeneTreeiENSGT00550000074559.
    HOGENOMiHOG000115733.
    HOVERGENiHBG108324.
    InParanoidiP04906.
    KOiK00799.
    OMAiLRCKYAT.
    OrthoDBiEOG7KH9M3.
    PhylomeDBiP04906.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003082. GST_pi.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01268. GSTRNSFRASEP.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04906-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPYTIVYFP VRGRCEATRM LLADQGQSWK EEVVTIDVWL QGSLKSTCLY    50
    GQLPKFEDGD LTLYQSNAIL RHLGRSLGLY GKDQKEAALV DMVNDGVEDL 100
    RCKYGTLIYT NYENGKDDYV KALPGHLKPF ETLLSQNQGG KAFIVGNQIS 150
    FADYNLLDLL LVHQVLAPGC LDNFPLLSAY VARLSARPKI KAFLSSPDHL 200
    NRPINGNGKQ 210
    Length:210
    Mass (Da):23,439
    Last modified:January 23, 2007 - v2
    Checksum:i9B71785D1FC28FDB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti15 – 151C → Y AA sequence (PubMed:3359441)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02904 mRNA. Translation: CAA26664.1.
    L29427 Genomic DNA. Translation: AAB59718.1.
    BC058439 mRNA. Translation: AAH58439.1.
    BC058440 mRNA. Translation: AAH58440.1.
    PIRiA26546. XURTGP.
    S59902.
    RefSeqiNP_036709.1. NM_012577.2.
    UniGeneiRn.87063.

    Genome annotation databases

    EnsembliENSRNOT00000024601; ENSRNOP00000024601; ENSRNOG00000018237.
    GeneIDi24426.
    KEGGirno:24426.
    UCSCiRGD:2758. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02904 mRNA. Translation: CAA26664.1 .
    L29427 Genomic DNA. Translation: AAB59718.1 .
    BC058439 mRNA. Translation: AAH58439.1 .
    BC058440 mRNA. Translation: AAH58440.1 .
    PIRi A26546. XURTGP.
    S59902.
    RefSeqi NP_036709.1. NM_012577.2.
    UniGenei Rn.87063.

    3D structure databases

    ProteinModelPortali P04906.
    SMRi P04906. Positions 2-210.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 246591. 1 interaction.
    IntActi P04906. 1 interaction.

    PTM databases

    PhosphoSitei P04906.

    2D gel databases

    World-2DPAGE 0004:P04906.

    Proteomic databases

    PaxDbi P04906.
    PRIDEi P04906.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000024601 ; ENSRNOP00000024601 ; ENSRNOG00000018237 .
    GeneIDi 24426.
    KEGGi rno:24426.
    UCSCi RGD:2758. rat.

    Organism-specific databases

    CTDi 2950.
    RGDi 2758. Gstp1.

    Phylogenomic databases

    eggNOGi NOG05174.
    GeneTreei ENSGT00550000074559.
    HOGENOMi HOG000115733.
    HOVERGENi HBG108324.
    InParanoidi P04906.
    KOi K00799.
    OMAi LRCKYAT.
    OrthoDBi EOG7KH9M3.
    PhylomeDBi P04906.
    TreeFami TF105321.

    Miscellaneous databases

    NextBioi 603297.
    PMAP-CutDB P04906.
    PROi P04906.

    Gene expression databases

    ArrayExpressi P04906.
    Genevestigatori P04906.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003082. GST_pi.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01268. GSTRNSFRASEP.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA."
      Suguoka Y., Kano T., Okuda A., Sakai M., Kitagawa T., Muramatsu M.
      Nucleic Acids Res. 13:6049-6057(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The structure of the rat glutathione S-transferase P gene and related pseudogenes."
      Okuda A., Sakai M., Muramatsu M.
      J. Biol. Chem. 262:3858-3863(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Pituitary.
    4. "Purification and characterization of P-52 (glutathione S-transferase-P or 7-7) from normal liver and putative preneoplastic liver nodules."
      Rushmore T.H., Harris L., Nagai M., Sharma R.N., Hayes M.A., Cameron R.G., Murray R.K., Farber E.
      Cancer Res. 48:2805-2812(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-27.
    5. Lubec G., Afjehi-Sadat L., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-12; 56-71; 122-141 AND 192-209, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus and Spinal cord.
    6. "Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties."
      Mannervik B., Alin P., Guthenberg C., Jensson H., Tahir M.K., Warholm M., Joernvall H.
      Proc. Natl. Acad. Sci. U.S.A. 82:7202-7206(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-11.

    Entry informationi

    Entry nameiGSTP1_RAT
    AccessioniPrimary (citable) accession number: P04906
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3