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Protein

Glutathione S-transferase P

Gene

Gstp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration (By similarity).By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei8 – 81GlutathioneBy similarity
Binding sitei14 – 141GlutathioneBy similarity
Binding sitei39 – 391GlutathioneBy similarity
Binding sitei45 – 451GlutathioneBy similarity

GO - Molecular functioni

  • drug binding Source: RGD
  • glutathione binding Source: RGD
  • glutathione transferase activity Source: RGD
  • JUN kinase binding Source: BHF-UCL
  • kinase regulator activity Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_316307. Glutathione conjugation.
REACT_317923. Detoxification of Reactive Oxygen Species.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase P (EC:2.5.1.18)
Alternative name(s):
Chain 7
GST 7-7
GST class-pi
Gene namesi
Name:Gstp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi2758. Gstp1.

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion By similarity
  • Nucleus By similarity

  • Note: The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.By similarity

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: RGD
  • intracellular Source: BHF-UCL
  • mitochondrion Source: UniProtKB-SubCell
  • nucleus Source: RGD
  • plasma membrane Source: RGD
  • protein complex Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 210209Glutathione S-transferase PPRO_0000185907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41Phosphotyrosine; by EGFRBy similarity
Modified residuei103 – 1031N6-succinyllysineBy similarity
Modified residuei116 – 1161N6-succinyllysineBy similarity
Modified residuei128 – 1281N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP04906.
PRIDEiP04906.

2D gel databases

World-2DPAGE0004:P04906.

PTM databases

PhosphoSiteiP04906.

Miscellaneous databases

PMAP-CutDBP04906.

Expressioni

Tissue specificityi

Present in kidney, lung, testis and placenta, very low levels in liver.

Gene expression databases

ExpressionAtlasiP04906. baseline and differential.
GenevisibleiP04906. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with CDK5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi246591. 1 interaction.
IntActiP04906. 1 interaction.
STRINGi10116.ENSRNOP00000024601.

Structurei

3D structure databases

ProteinModelPortaliP04906.
SMRiP04906. Positions 2-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 8180GST N-terminalAdd
BLAST
Domaini83 – 204122GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 532Glutathione bindingBy similarity
Regioni65 – 662Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Pi family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG05174.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115733.
HOVERGENiHBG108324.
InParanoidiP04906.
KOiK00799.
OMAiLRCKYAT.
OrthoDBiEOG7KH9M3.
PhylomeDBiP04906.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003082. GST_pi.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01268. GSTRNSFRASEP.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPYTIVYFP VRGRCEATRM LLADQGQSWK EEVVTIDVWL QGSLKSTCLY
60 70 80 90 100
GQLPKFEDGD LTLYQSNAIL RHLGRSLGLY GKDQKEAALV DMVNDGVEDL
110 120 130 140 150
RCKYGTLIYT NYENGKDDYV KALPGHLKPF ETLLSQNQGG KAFIVGNQIS
160 170 180 190 200
FADYNLLDLL LVHQVLAPGC LDNFPLLSAY VARLSARPKI KAFLSSPDHL
210
NRPINGNGKQ
Length:210
Mass (Da):23,439
Last modified:January 23, 2007 - v2
Checksum:i9B71785D1FC28FDB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 151C → Y AA sequence (PubMed:3359441).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02904 mRNA. Translation: CAA26664.1.
L29427 Genomic DNA. Translation: AAB59718.1.
BC058439 mRNA. Translation: AAH58439.1.
BC058440 mRNA. Translation: AAH58440.1.
PIRiA26546. XURTGP.
S59902.
RefSeqiNP_036709.1. NM_012577.2.
UniGeneiRn.87063.

Genome annotation databases

EnsembliENSRNOT00000024601; ENSRNOP00000024601; ENSRNOG00000018237.
GeneIDi24426.
KEGGirno:24426.
UCSCiRGD:2758. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02904 mRNA. Translation: CAA26664.1.
L29427 Genomic DNA. Translation: AAB59718.1.
BC058439 mRNA. Translation: AAH58439.1.
BC058440 mRNA. Translation: AAH58440.1.
PIRiA26546. XURTGP.
S59902.
RefSeqiNP_036709.1. NM_012577.2.
UniGeneiRn.87063.

3D structure databases

ProteinModelPortaliP04906.
SMRiP04906. Positions 2-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246591. 1 interaction.
IntActiP04906. 1 interaction.
STRINGi10116.ENSRNOP00000024601.

PTM databases

PhosphoSiteiP04906.

2D gel databases

World-2DPAGE0004:P04906.

Proteomic databases

PaxDbiP04906.
PRIDEiP04906.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024601; ENSRNOP00000024601; ENSRNOG00000018237.
GeneIDi24426.
KEGGirno:24426.
UCSCiRGD:2758. rat.

Organism-specific databases

CTDi2950.
RGDi2758. Gstp1.

Phylogenomic databases

eggNOGiNOG05174.
GeneTreeiENSGT00550000074559.
HOGENOMiHOG000115733.
HOVERGENiHBG108324.
InParanoidiP04906.
KOiK00799.
OMAiLRCKYAT.
OrthoDBiEOG7KH9M3.
PhylomeDBiP04906.
TreeFamiTF105321.

Enzyme and pathway databases

ReactomeiREACT_316307. Glutathione conjugation.
REACT_317923. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

NextBioi603297.
PMAP-CutDBP04906.
PROiP04906.

Gene expression databases

ExpressionAtlasiP04906. baseline and differential.
GenevisibleiP04906. RN.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR003082. GST_pi.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01268. GSTRNSFRASEP.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA."
    Suguoka Y., Kano T., Okuda A., Sakai M., Kitagawa T., Muramatsu M.
    Nucleic Acids Res. 13:6049-6057(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The structure of the rat glutathione S-transferase P gene and related pseudogenes."
    Okuda A., Sakai M., Muramatsu M.
    J. Biol. Chem. 262:3858-3863(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  4. "Purification and characterization of P-52 (glutathione S-transferase-P or 7-7) from normal liver and putative preneoplastic liver nodules."
    Rushmore T.H., Harris L., Nagai M., Sharma R.N., Hayes M.A., Cameron R.G., Murray R.K., Farber E.
    Cancer Res. 48:2805-2812(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-27.
  5. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-12; 56-71; 122-141 AND 192-209, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.
  6. "Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties."
    Mannervik B., Alin P., Guthenberg C., Jensson H., Tahir M.K., Warholm M., Joernvall H.
    Proc. Natl. Acad. Sci. U.S.A. 82:7202-7206(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-11.

Entry informationi

Entry nameiGSTP1_RAT
AccessioniPrimary (citable) accession number: P04906
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.