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Protein

Glutathione S-transferase alpha-3

Gene

Gsta3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91GlutathioneBy similarity
Binding sitei45 – 451GlutathioneBy similarity

GO - Molecular functioni

  1. drug binding Source: RGD
  2. glutathione binding Source: RGD
  3. glutathione transferase activity Source: RGD

GO - Biological processi

  1. aging Source: RGD
  2. response to drug Source: RGD
  3. response to nutrient levels Source: RGD
  4. xenobiotic catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiREACT_251519. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase alpha-3 (EC:2.5.1.18)
Alternative name(s):
GST 2-2
GST A3-3
GST AA
Glutathione S-transferase Yc-1
Short name:
GST Yc1
Gene namesi
Name:Gsta3
Synonyms:Gstyc1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi2753. Gsta3.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RGD
  2. nuclear outer membrane Source: RGD
  3. nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 221220Glutathione S-transferase alpha-3PRO_0000185794Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41N6-succinyllysineBy similarity

Proteomic databases

PaxDbiP04904.
PRIDEiP04904.

2D gel databases

World-2DPAGE0004:P04904.

PTM databases

PhosphoSiteiP04904.

Expressioni

Developmental stagei

Liver from adult female rats contains about 2-fold greater levels of YC1 than is found in liver from adult male rats.

Gene expression databases

ExpressionAtlasiP04904. baseline and differential.
GenevestigatoriP04904.

Interactioni

Subunit structurei

Heterodimer of YC1 and YC2.

Protein-protein interaction databases

MINTiMINT-4565454.

Structurei

3D structure databases

ProteinModelPortaliP04904.
SMRiP04904. Positions 4-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8381GST N-terminalAdd
BLAST
Domaini85 – 207123GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni54 – 552Glutathione bindingBy similarity
Regioni67 – 682Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOVERGENiHBG053749.
KOiK00799.
OMAiNVESAKC.
OrthoDBiEOG79CZ0K.
PhylomeDBiP04904.
TreeFamiTF105321.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04904-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA
110 120 130 140 150
DLDEIVLHYP YIPPGEKEAS LAKIKDKARN RYFPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLSRADVYL VQVLYHVEEL DPSALANFPL LKALRTRVSN LPTVKKFLQP
210 220
GSQRKPLEDE KCVESAVKIF S
Length:221
Mass (Da):25,319
Last modified:January 23, 2007 - v3
Checksum:iC45C5AF03573707F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021L → I(PubMed:2985614)Curated
Sequence conflicti184 – 1841L → K(PubMed:2985614)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78848 mRNA. Translation: CAA55405.1.
S72505 mRNA. Translation: AAP21064.1.
K01932 mRNA. Translation: AAA41294.1.
AF111160 mRNA. Translation: AAD28714.1.
BC059128 mRNA. Translation: AAH59128.1.
BC088127 mRNA. Translation: AAH88127.1.
PIRiA26753.
RefSeqiNP_113697.1. NM_031509.2.
XP_006226824.1. XM_006226762.1.
XP_006244721.2. XM_006244659.2.
UniGeneiRn.10460.

Genome annotation databases

EnsembliENSRNOT00000018325; ENSRNOP00000018325; ENSRNOG00000013484.
GeneIDi102550391.
24421.
KEGGirno:102550391.
rno:24421.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78848 mRNA. Translation: CAA55405.1.
S72505 mRNA. Translation: AAP21064.1.
K01932 mRNA. Translation: AAA41294.1.
AF111160 mRNA. Translation: AAD28714.1.
BC059128 mRNA. Translation: AAH59128.1.
BC088127 mRNA. Translation: AAH88127.1.
PIRiA26753.
RefSeqiNP_113697.1. NM_031509.2.
XP_006226824.1. XM_006226762.1.
XP_006244721.2. XM_006244659.2.
UniGeneiRn.10460.

3D structure databases

ProteinModelPortaliP04904.
SMRiP04904. Positions 4-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4565454.

Chemistry

ChEMBLiCHEMBL4436.

PTM databases

PhosphoSiteiP04904.

2D gel databases

World-2DPAGE0004:P04904.

Proteomic databases

PaxDbiP04904.
PRIDEiP04904.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000018325; ENSRNOP00000018325; ENSRNOG00000013484.
GeneIDi102550391.
24421.
KEGGirno:102550391.
rno:24421.

Organism-specific databases

CTDi2938.
RGDi2753. Gsta3.

Phylogenomic databases

eggNOGiNOG266414.
GeneTreeiENSGT00670000097856.
HOVERGENiHBG053749.
KOiK00799.
OMAiNVESAKC.
OrthoDBiEOG79CZ0K.
PhylomeDBiP04904.
TreeFamiTF105321.

Enzyme and pathway databases

ReactomeiREACT_251519. Glutathione conjugation.

Miscellaneous databases

NextBioi603279.

Gene expression databases

ExpressionAtlasiP04904. baseline and differential.
GenevestigatoriP04904.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1."
    Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E.
    J. Biol. Chem. 269:20707-20717(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer 344.
    Tissue: Liver.
  2. "Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit."
    Telakowski-Hopkins C.A., Rodkey K.A., Bennett C.D., Lu A.Y.H., Pickett C.B.
    J. Biol. Chem. 260:5820-5825(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Genomic cloning and characterization of the rat glutathione S-transferase-A3-subunit gene."
    Fotouhi-Ardakani N., Batist G.
    Biochem. J. 339:685-693(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver and Pituitary.
  5. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-13; 21-36; 70-78; 142-152; 156-182; 188-195 AND 197-204, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.
  6. "The Yc and Ya subunits of rat liver glutathione S-transferases are the products of separate genes."
    Tu C.-P.D., Lai H.-C.J., Li N.-Q., Weiss M.J., Reddy C.C.
    J. Biol. Chem. 259:9434-9439(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-221.
  7. "Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide."
    Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E.
    Biochem. J. 279:385-398(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Strain: Fischer 344.
    Tissue: Liver.
  8. "Expression in Escherichia coli of rat liver cytosolic glutathione S-transferase Yc cDNA."
    Huskey S.E., Wang R.W., Linemeyer D.L., Pickett C.B., Lu A.Y.H.
    Arch. Biochem. Biophys. 279:116-121(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-31.

Entry informationi

Entry nameiGSTA3_RAT
AccessioniPrimary (citable) accession number: P04904
Secondary accession number(s): Q6LD92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.