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P04904

- GSTA3_RAT

UniProt

P04904 - GSTA3_RAT

Protein

Glutathione S-transferase alpha-3

Gene

Gsta3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91GlutathioneBy similarity
    Binding sitei45 – 451GlutathioneBy similarity

    GO - Molecular functioni

    1. drug binding Source: RGD
    2. glutathione binding Source: RGD
    3. glutathione transferase activity Source: RGD

    GO - Biological processi

    1. aging Source: RGD
    2. response to drug Source: RGD
    3. response to nutrient levels Source: RGD
    4. xenobiotic catabolic process Source: RGD

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase alpha-3 (EC:2.5.1.18)
    Alternative name(s):
    GST 2-2
    GST A3-3
    GST AA
    Glutathione S-transferase Yc-1
    Short name:
    GST Yc1
    Gene namesi
    Name:Gsta3
    Synonyms:Gstyc1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi2753. Gsta3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD
    2. nuclear outer membrane Source: RGD
    3. nucleus Source: RGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 221220Glutathione S-transferase alpha-3PRO_0000185794Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei4 – 41N6-succinyllysineBy similarity

    Proteomic databases

    PaxDbiP04904.
    PRIDEiP04904.

    2D gel databases

    World-2DPAGE0004:P04904.

    PTM databases

    PhosphoSiteiP04904.

    Expressioni

    Developmental stagei

    Liver from adult female rats contains about 2-fold greater levels of YC1 than is found in liver from adult male rats.

    Gene expression databases

    ArrayExpressiP04904.
    GenevestigatoriP04904.

    Interactioni

    Subunit structurei

    Heterodimer of YC1 and YC2.

    Protein-protein interaction databases

    MINTiMINT-4565454.

    Structurei

    3D structure databases

    ProteinModelPortaliP04904.
    SMRiP04904. Positions 4-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 8381GST N-terminalAdd
    BLAST
    Domaini85 – 207123GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 552Glutathione bindingBy similarity
    Regioni67 – 682Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Alpha family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG266414.
    GeneTreeiENSGT00670000097856.
    HOVERGENiHBG053749.
    InParanoidiP04904.
    KOiK00799.
    OMAiHESGSHY.
    OrthoDBiEOG79CZ0K.
    PhylomeDBiP04904.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01266. GSTRNSFRASEA.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04904-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL    50
    MFQQVPMVEI DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA 100
    DLDEIVLHYP YIPPGEKEAS LAKIKDKARN RYFPAFEKVL KSHGQDYLVG 150
    NRLSRADVYL VQVLYHVEEL DPSALANFPL LKALRTRVSN LPTVKKFLQP 200
    GSQRKPLEDE KCVESAVKIF S 221
    Length:221
    Mass (Da):25,319
    Last modified:January 23, 2007 - v3
    Checksum:iC45C5AF03573707F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021L → I(PubMed:2985614)Curated
    Sequence conflicti184 – 1841L → K(PubMed:2985614)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78848 mRNA. Translation: CAA55405.1.
    S72505 mRNA. Translation: AAP21064.1.
    K01932 mRNA. Translation: AAA41294.1.
    AF111160 mRNA. Translation: AAD28714.1.
    BC059128 mRNA. Translation: AAH59128.1.
    BC088127 mRNA. Translation: AAH88127.1.
    PIRiA26753.
    RefSeqiNP_113697.1. NM_031509.2.
    XP_006226824.1. XM_006226762.1.
    UniGeneiRn.10460.

    Genome annotation databases

    EnsembliENSRNOT00000018325; ENSRNOP00000018325; ENSRNOG00000013484.
    GeneIDi102550391.
    24421.
    KEGGirno:102550391.
    rno:24421.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78848 mRNA. Translation: CAA55405.1 .
    S72505 mRNA. Translation: AAP21064.1 .
    K01932 mRNA. Translation: AAA41294.1 .
    AF111160 mRNA. Translation: AAD28714.1 .
    BC059128 mRNA. Translation: AAH59128.1 .
    BC088127 mRNA. Translation: AAH88127.1 .
    PIRi A26753.
    RefSeqi NP_113697.1. NM_031509.2.
    XP_006226824.1. XM_006226762.1.
    UniGenei Rn.10460.

    3D structure databases

    ProteinModelPortali P04904.
    SMRi P04904. Positions 4-220.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4565454.

    Chemistry

    BindingDBi P04904.
    ChEMBLi CHEMBL4436.

    PTM databases

    PhosphoSitei P04904.

    2D gel databases

    World-2DPAGE 0004:P04904.

    Proteomic databases

    PaxDbi P04904.
    PRIDEi P04904.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000018325 ; ENSRNOP00000018325 ; ENSRNOG00000013484 .
    GeneIDi 102550391.
    24421.
    KEGGi rno:102550391.
    rno:24421.

    Organism-specific databases

    CTDi 2938.
    RGDi 2753. Gsta3.

    Phylogenomic databases

    eggNOGi NOG266414.
    GeneTreei ENSGT00670000097856.
    HOVERGENi HBG053749.
    InParanoidi P04904.
    KOi K00799.
    OMAi HESGSHY.
    OrthoDBi EOG79CZ0K.
    PhylomeDBi P04904.
    TreeFami TF105321.

    Miscellaneous databases

    NextBioi 603279.

    Gene expression databases

    ArrayExpressi P04904.
    Genevestigatori P04904.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR003080. GST_alpha.
    IPR004046. GST_C.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01266. GSTRNSFRASEA.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1."
      Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E.
      J. Biol. Chem. 269:20707-20717(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Fischer 344.
      Tissue: Liver.
    2. "Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit."
      Telakowski-Hopkins C.A., Rodkey K.A., Bennett C.D., Lu A.Y.H., Pickett C.B.
      J. Biol. Chem. 260:5820-5825(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Genomic cloning and characterization of the rat glutathione S-transferase-A3-subunit gene."
      Fotouhi-Ardakani N., Batist G.
      Biochem. J. 339:685-693(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Fischer.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver and Pituitary.
    5. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
      Submitted (JUL-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-13; 21-36; 70-78; 142-152; 156-182; 188-195 AND 197-204, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.
    6. "The Yc and Ya subunits of rat liver glutathione S-transferases are the products of separate genes."
      Tu C.-P.D., Lai H.-C.J., Li N.-Q., Weiss M.J., Reddy C.C.
      J. Biol. Chem. 259:9434-9439(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-221.
    7. "Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide."
      Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E.
      Biochem. J. 279:385-398(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Strain: Fischer 344.
      Tissue: Liver.
    8. "Expression in Escherichia coli of rat liver cytosolic glutathione S-transferase Yc cDNA."
      Huskey S.E., Wang R.W., Linemeyer D.L., Pickett C.B., Lu A.Y.H.
      Arch. Biochem. Biophys. 279:116-121(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-31.

    Entry informationi

    Entry nameiGSTA3_RAT
    AccessioniPrimary (citable) accession number: P04904
    Secondary accession number(s): Q6LD92
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3