P04904 (GSTA3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase alpha-3
EC=2.5.1.18

Alternative name(s):
GST 2-2
GST A3-3
GST AA
Glutathione S-transferase Yc-1
Short name=GST Yc1

Gene names

Name:	Gsta3
Synonyms:	Gstyc1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Heterodimer of YC1 and YC2.
Subcellular location	Cytoplasm.
Developmental stage	Liver from adult female rats contains about 2-fold greater levels of YC1 than is found in liver from adult male rats.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5 Ref.8
	Chain	2 – 221	220	Glutathione S-transferase alpha-3		PRO_0000185794
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 207	123	GST C-terminal
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity
	Binding site	45	1	Glutathione By similarity
Experimental info
	Sequence conflict	102	1	L → I Ref.2
	Sequence conflict	184	1	L → K Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P04904 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C45C5AF03573707F	FASTA	221	25,319
	10 20 30 40 50 60 MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL MFQQVPMVEI 70 80 90 100 110 120 DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA DLDEIVLHYP YIPPGEKEAS 130 140 150 160 170 180 LAKIKDKARN RYFPAFEKVL KSHGQDYLVG NRLSRADVYL VQVLYHVEEL DPSALANFPL 190 200 210 220 LKALRTRVSN LPTVKKFLQP GSQRKPLEDE KCVESAVKIF S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1." Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E. J. Biol. Chem. 269:20707-20717(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344. Tissue: Liver.
[2]	"Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit." Telakowski-Hopkins C.A., Rodkey K.A., Bennett C.D., Lu A.Y.H., Pickett C.B. J. Biol. Chem. 260:5820-5825(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genomic cloning and characterization of the rat glutathione S-transferase-A3-subunit gene." Fotouhi-Ardakani N., Batist G. Biochem. J. 339:685-693(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Pituitary.
[5]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-13; 21-36; 70-78; 142-152; 156-182; 188-195 AND 197-204, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[6]	"The Yc and Ya subunits of rat liver glutathione S-transferases are the products of separate genes." Tu C.-P.D., Lai H.-C.J., Li N.-Q., Weiss M.J., Reddy C.C. J. Biol. Chem. 259:9434-9439(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-221.
[7]	"Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide." Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E. Biochem. J. 279:385-398(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: Fischer 344. Tissue: Liver.
[8]	"Expression in Escherichia coli of rat liver cytosolic glutathione S-transferase Yc cDNA." Huskey S.E., Wang R.W., Linemeyer D.L., Pickett C.B., Lu A.Y.H. Arch. Biochem. Biophys. 279:116-121(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78848 mRNA. Translation: CAA55405.1. S72505 mRNA. Translation: AAP21064.1. K01932 mRNA. Translation: AAA41294.1. AF111160 mRNA. Translation: AAD28714.1. BC059128 mRNA. Translation: AAH59128.1. BC088127 mRNA. Translation: AAH88127.1.
IPI	IPI00231150.
PIR	A26753.
RefSeq	NP_113697.1. NM_031509.2. XP_003754545.1. XM_003754497.1.
UniGene	Rn.10460.
3D structure databases
ProteinModelPortal	P04904.
SMR	P04904. Positions 4-220.
ModBase	Search...
PTM databases
PhosphoSite	P04904.
2D gel databases
World-2DPAGE	0004:P04904.
Proteomic databases
PaxDb	P04904.
PRIDE	P04904.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018325; ENSRNOP00000018325; ENSRNOG00000013484.
GeneID	24421.
KEGG	rno:24421.
Organism-specific databases
CTD	2940.
RGD	2753. Gsta3.
Phylogenomic databases
eggNOG	NOG266414.
GeneTree	ENSGT00670000097856.
HOVERGEN	HBG053749.
InParanoid	P04904.
KO	K00799.
OMA	HEDNISI.
OrthoDB	EOG479F80.
Gene expression databases
ArrayExpress	P04904.
Genevestigator	P04904.
GermOnline	ENSRNOG00000013484. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11571:SF4. PTHR11571:SF4. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P04904.
ChEMBL	CHEMBL4436.
NextBio	603279.

Entry information

Entry name

GSTA3_RAT

Accession

Primary (citable) accession number: P04904
Secondary accession number(s): Q6LD92

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 116 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program