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Reviewed, UniProtKB/Swiss-Prot P04904 (GSTA3_RAT)

Last modified June 16, 2009. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glutathione S-transferase alpha-3
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase Yc-1
      Short name=GST Yc1
    GST 2-2
    GST AA
    GST A3-3
Gene names
Name: Gsta3
Synonyms: Gstyc1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subunit structure

Heterodimer of YC1 and YC2.

Subcellular location

Cytoplasm.

Developmental stage

Liver from adult female rats contains about 2-fold greater levels of YC1 than is found in liver from adult male rats.

Sequence similarities

Belongs to the GST superfamily. Alpha family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5 Ref.8
Chain2 – 221220Glutathione S-transferase alpha-3
PRO_0000185794

Regions

Domain3 – 8381GST N-terminal
Domain85 – 207123GST C-terminal

Experimental info

Sequence conflict1021L → I Ref.2
Sequence conflict1841L → K Ref.2

Sequences

Sequence LengthMass (Da)Tools
P04904-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: C45C5AF03573707F

FASTA22125,319
        10         20         30         40         50         60 
MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL MFQQVPMVEI 

        70         80         90        100        110        120 
DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA DLDEIVLHYP YIPPGEKEAS 

       130        140        150        160        170        180 
LAKIKDKARN RYFPAFEKVL KSHGQDYLVG NRLSRADVYL VQVLYHVEEL DPSALANFPL 

       190        200        210        220 
LKALRTRVSN LPTVKKFLQP GSQRKPLEDE KCVESAVKIF S 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1."
Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E.
J. Biol. Chem. 269:20707-20717(1994) [PubMed: 8051171] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Fischer 344.
Tissue: Liver.
[2]"Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit."
Telakowski-Hopkins C.A., Rodkey K.A., Bennett C.D., Lu A.Y.H., Pickett C.B.
J. Biol. Chem. 260:5820-5825(1985) [PubMed: 2985614] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Genomic cloning and characterization of the rat glutathione S-transferase-A3-subunit gene."
Fotouhi-Ardakani N., Batist G.
Biochem. J. 339:685-693(1999) [PubMed: 10215608] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Fischer.
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver and Pituitary.
[5]Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.
Submitted (JUL-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 2-13; 21-36; 70-78; 142-152; 156-182; 188-195 AND 197-204, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Brain, Hippocampus and Spinal cord.
[6]"The Yc and Ya subunits of rat liver glutathione S-transferases are the products of separate genes."
Tu C.-P.D., Lai H.-C.J., Li N.-Q., Weiss M.J., Reddy C.C.
J. Biol. Chem. 259:9434-9439(1984) [PubMed: 6204982] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-221.
[7]"Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide."
Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E.
Biochem. J. 279:385-398(1991) [PubMed: 1953636] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
Strain: Fischer 344.
Tissue: Liver.
[8]"Expression in Escherichia coli of rat liver cytosolic glutathione S-transferase Yc cDNA."
Huskey S.E., Wang R.W., Linemeyer D.L., Pickett C.B., Lu A.Y.H.
Arch. Biochem. Biophys. 279:116-121(1990) [PubMed: 2186703] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-31.
+Additional computationally mapped references.

Cross-references

Sequence databases

X78848 mRNA. Translation: CAA55405.1.
S72505 mRNA. Translation: AAP21064.1.
K01932 mRNA. Translation: AAA41294.1.
AF111160 mRNA. Translation: AAD28714.1.
BC059128 mRNA. Translation: AAH59128.1.
BC088127 mRNA. Translation: AAH88127.1.
IPIIPI00231150.
PIRA26753.
RefSeqNP_113697.1.
UniGeneRn.10460

3D structure databases

HSSPHSSP built from PDB template 1K3O based on UniProtKB P08263.
SMRP04904. Positions 4-220.
ModBaseSearch...

PTM databases

PhosphoSiteP04904.

Proteomic databases

PRIDEP04904.

Genome annotation databases

EnsemblENSRNOG00000013484. Rattus norvegicus. [Contig view]
GeneID24421.
KEGGrno:24421.
NMPDRfig|10116.3.peg.29788.

Organism-specific databases

RGD2753. Gsta3.

Phylogenomic databases

HOVERGENP04904.
OMAP04904. HEDNISI.

Enzyme and pathway databases

BRENDA2.5.1.18. 248.

Gene expression databases

ArrayExpressP04904.
GermOnlineENSRNOG00000013484. Rattus norvegicus.

Family and domain databases

InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012335. Thioredoxin_fold.
[Graphical view]
Gene3DG3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHERPTHR11571:SF4. GST_alpha. 1 hit.
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSPR01266. GSTRNSFRASEA.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio603279.

Entry information

Entry nameGSTA3_RAT
AccessionPrimary (citable) accession number: P04904
Secondary accession number(s): Q6LD92
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents