Reviewed, UniProtKB/Swiss-Prot P04904 (GSTA3_RAT)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase alpha-3
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase Yc-1
      Short name=GST Yc1
    GST 2-2
    GST AA
    GST A3-3

Gene names

Name:	Gsta3
Synonyms:	Gstyc1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Heterodimer of YC1 and YC2.
Subcellular location	Cytoplasm.
Developmental stage	Liver from adult female rats contains about 2-fold greater levels of YC1 than is found in liver from adult male rats.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to drug Ref.3 Non-traceable author statement. Source: RGD xenobiotic catabolic process Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD nuclear outer membrane Inferred from direct assay. Source: RGD
Molecular function	drug binding Inferred from direct assay. Source: RGD glutathione binding Inferred from direct assay. Source: RGD glutathione transferase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5 Ref.8
	Chain	2 – 221	220	Glutathione S-transferase alpha-3		PRO_0000185794
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 207	123	GST C-terminal
Experimental info
	Sequence conflict	102	1	L → I Ref.2
	Sequence conflict	184	1	L → K Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P04904-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: C45C5AF03573707F	FASTA	221	25,319
	10 20 30 40 50 60 MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL MFQQVPMVEI 70 80 90 100 110 120 DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA DLDEIVLHYP YIPPGEKEAS 130 140 150 160 170 180 LAKIKDKARN RYFPAFEKVL KSHGQDYLVG NRLSRADVYL VQVLYHVEEL DPSALANFPL 190 200 210 220 LKALRTRVSN LPTVKKFLQP GSQRKPLEDE KCVESAVKIF S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1." Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E. J. Biol. Chem. 269:20707-20717(1994) [PubMed: 8051171] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344. Tissue: Liver.
[2]	"Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit." Telakowski-Hopkins C.A., Rodkey K.A., Bennett C.D., Lu A.Y.H., Pickett C.B. J. Biol. Chem. 260:5820-5825(1985) [PubMed: 2985614] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genomic cloning and characterization of the rat glutathione S-transferase-A3-subunit gene." Fotouhi-Ardakani N., Batist G. Biochem. J. 339:685-693(1999) [PubMed: 10215608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Pituitary.
[5]	Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-13; 21-36; 70-78; 142-152; 156-182; 188-195 AND 197-204, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[6]	"The Yc and Ya subunits of rat liver glutathione S-transferases are the products of separate genes." Tu C.-P.D., Lai H.-C.J., Li N.-Q., Weiss M.J., Reddy C.C. J. Biol. Chem. 259:9434-9439(1984) [PubMed: 6204982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-221.
[7]	"Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide." Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E. Biochem. J. 279:385-398(1991) [PubMed: 1953636] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: Fischer 344. Tissue: Liver.
[8]	"Expression in Escherichia coli of rat liver cytosolic glutathione S-transferase Yc cDNA." Huskey S.E., Wang R.W., Linemeyer D.L., Pickett C.B., Lu A.Y.H. Arch. Biochem. Biophys. 279:116-121(1990) [PubMed: 2186703] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X78848 mRNA. Translation: CAA55405.1. S72505 mRNA. Translation: AAP21064.1. K01932 mRNA. Translation: AAA41294.1. AF111160 mRNA. Translation: AAD28714.1. BC059128 mRNA. Translation: AAH59128.1. BC088127 mRNA. Translation: AAH88127.1.
IPI	IPI00231150.
PIR	A26753.
RefSeq	NP_113697.1.
UniGene	Rn.10460
3D structure databases
HSSP	HSSP built from PDB template 1K3O based on UniProtKB P08263.
SMR	P04904. Positions 4-220.
ModBase	Search...
PTM databases
PhosphoSite	P04904.
Proteomic databases
PRIDE	P04904.
Genome annotation databases
Ensembl	ENSRNOG00000013484. Rattus norvegicus. [Contig view]
GeneID	24421.
KEGG	rno:24421.
NMPDR	fig|10116.3.peg.29788.
Organism-specific databases
RGD	2753. Gsta3.
Phylogenomic databases
HOVERGEN	P04904.
OMA	P04904. HEDNISI.
Enzyme and pathway databases
BRENDA	2.5.1.18. 248.
Gene expression databases
ArrayExpress	P04904.
GermOnline	ENSRNOG00000013484. Rattus norvegicus.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	603279.

Entry information

Entry name

GSTA3_RAT

Accession

Primary (citable) accession number: P04904
Secondary accession number(s): Q6LD92

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 87 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)