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Protein

Glutathione S-transferase alpha-3

Gene

Gsta3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has substantial activity toward aflatoxin B1-8,9-epoxide.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1
Binding sitei45GlutathioneBy similarity1

GO - Molecular functioni

  • drug binding Source: RGD
  • glutathione binding Source: CAFA
  • glutathione peroxidase activity Source: RGD
  • glutathione transferase activity Source: CAFA

GO - Biological processi

  • aging Source: RGD
  • epithelial cell differentiation Source: RGD
  • glutathione metabolic process Source: RGD
  • linoleic acid metabolic process Source: RGD
  • response to drug Source: RGD
  • response to nutrient levels Source: RGD
  • xenobiotic catabolic process Source: CAFA

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.5.1.18. 5301.
ReactomeiR-RNO-156590. Glutathione conjugation.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase alpha-3 (EC:2.5.1.18)
Alternative name(s):
GST 2-2
GST A3-3
GST AA
Glutathione S-transferase Yc-1
Short name:
GST Yc1
Gene namesi
Name:Gsta3
Synonyms:Gstyc1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi2753. Gsta3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4436.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001857942 – 221Glutathione S-transferase alpha-3Add BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4N6-succinyllysineBy similarity1

Proteomic databases

PaxDbiP04904.
PRIDEiP04904.

2D gel databases

World-2DPAGEi0004:P04904.

PTM databases

iPTMnetiP04904.
PhosphoSitePlusiP04904.

Expressioni

Developmental stagei

Liver from adult female rats contains about 2-fold greater levels of YC1 than is found in liver from adult male rats.

Gene expression databases

BgeeiENSRNOG00000013484.
ExpressionAtlasiP04904. baseline and differential.
GenevisibleiP04904. RN.

Interactioni

Subunit structurei

Heterodimer of YC1 and YC2.

Protein-protein interaction databases

MINTiMINT-4565454.
STRINGi10116.ENSRNOP00000018325.

Structurei

3D structure databases

ProteinModelPortaliP04904.
SMRiP04904.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 207GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingBy similarity2
Regioni67 – 68Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
GeneTreeiENSGT00670000097856.
HOVERGENiHBG053749.
KOiK00799.
OMAiDAHYVET.
OrthoDBiEOG091G0O3D.
PhylomeDBiP04904.
TreeFamiTF105321.

Family and domain databases

InterProiView protein in InterPro
IPR010987. Glutathione-S-Trfase_C-like.
IPR036282. Glutathione-S-Trfase_C_sf.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR036249. Thioredoxin-like_sf.
PfamiView protein in Pfam
PF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiView protein in PROSITE
PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04904-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL
60 70 80 90 100
MFQQVPMVEI DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA
110 120 130 140 150
DLDEIVLHYP YIPPGEKEAS LAKIKDKARN RYFPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLSRADVYL VQVLYHVEEL DPSALANFPL LKALRTRVSN LPTVKKFLQP
210 220
GSQRKPLEDE KCVESAVKIF S
Length:221
Mass (Da):25,319
Last modified:January 23, 2007 - v3
Checksum:iC45C5AF03573707F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102L → I (PubMed:2985614).Curated1
Sequence conflicti184L → K (PubMed:2985614).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78848 mRNA. Translation: CAA55405.1.
S72505 mRNA. Translation: AAP21064.1.
K01932 mRNA. Translation: AAA41294.1.
AF111160 mRNA. Translation: AAD28714.1.
BC059128 mRNA. Translation: AAH59128.1.
BC088127 mRNA. Translation: AAH88127.1.
PIRiA26753.
RefSeqiNP_113697.1. NM_031509.2.
XP_017452251.1. XM_017596762.1.
UniGeneiRn.10460.

Genome annotation databases

EnsembliENSRNOT00000018325; ENSRNOP00000018325; ENSRNOG00000013484.
GeneIDi108348061.
24421.
KEGGirno:108348061.
rno:24421.

Similar proteinsi

Entry informationi

Entry nameiGSTA3_RAT
AccessioniPrimary (citable) accession number: P04904
Secondary accession number(s): Q6LD92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families