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Protein

Glutathione S-transferase alpha-2

Gene

Gsta2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1
Binding sitei45GlutathioneBy similarity1

GO - Molecular functioni

  • drug binding Source: RGD
  • glutathione binding Source: RGD
  • glutathione transferase activity Source: RGD

GO - Biological processi

  • aging Source: RGD
  • xenobiotic catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BRENDAi2.5.1.18. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase alpha-2 (EC:2.5.1.18)
Alternative name(s):
GST 1b-1b
GST A2-2
Glutathione S-transferase Ya-2
Short name:
GST Ya2
Gene namesi
Name:Gsta2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2754. Gsta2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • nuclear outer membrane Source: RGD
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001857932 – 222Glutathione S-transferase alpha-2Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4N6-succinyllysineBy similarity1

Proteomic databases

PaxDbiP04903.
PRIDEiP04903.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi268945. 1 interactor.
STRINGi10116.ENSRNOP00000043510.

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi57 – 60Combined sources4
Beta strandi63 – 65Combined sources3
Helixi86 – 104Combined sources19
Turni108 – 111Combined sources4
Helixi114 – 120Combined sources7
Helixi124 – 130Combined sources7
Helixi132 – 143Combined sources12
Beta strandi146 – 149Combined sources4
Helixi155 – 171Combined sources17
Turni172 – 177Combined sources6
Helixi179 – 189Combined sources11
Helixi192 – 198Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LCZX-ray2.33A/B54-65[»]
A/B86-213[»]
5LD0X-ray1.60A86-213[»]
ProteinModelPortaliP04903.
SMRiP04903.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 208GST C-terminalAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingBy similarity2
Regioni67 – 68Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP04903.
KOiK00799.
PhylomeDBiP04903.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04903-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGKPVLHYF NARGRMECIR WLLAAAGVEF EEKLIQSPED LEKLKKDGNL
60 70 80 90 100
MFDQVPMVEI DGMKLAQTRA ILNYIATKYD LYGKDMKERA LIDMYSEGIL
110 120 130 140 150
DLTEMIIQLV ICPPDQREAK TALAKDRTKN RYLPAFEKVL KSHGQDYLVG
160 170 180 190 200
NRLTRVDIHL LELLLYVEEF DASLLTSFPL LKAFKSRISS LPNVKKFLQP
210 220
GSQRKPAMDA KQIEEARKVF KF
Length:222
Mass (Da):25,559
Last modified:January 23, 2007 - v2
Checksum:iAA342417E9857A9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00136 mRNA. Translation: AAA41282.1.
M25891 mRNA. Translation: AAA41290.1.
M14991
, M14986, M14987, M14988, M14989, M14990 Genomic DNA. Translation: AAA41295.1.
X00520 mRNA. Translation: CAA25203.1.
M27446 mRNA. Translation: AAA41291.1.
PIRiA24735.
A26653.
A92479. XURTG.
RefSeqiNP_001010921.1. NM_001010921.1.
UniGeneiRn.40574.

Genome annotation databases

GeneIDi494499.
KEGGirno:494499.
UCSCiRGD:2754. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00136 mRNA. Translation: AAA41282.1.
M25891 mRNA. Translation: AAA41290.1.
M14991
, M14986, M14987, M14988, M14989, M14990 Genomic DNA. Translation: AAA41295.1.
X00520 mRNA. Translation: CAA25203.1.
M27446 mRNA. Translation: AAA41291.1.
PIRiA24735.
A26653.
A92479. XURTG.
RefSeqiNP_001010921.1. NM_001010921.1.
UniGeneiRn.40574.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5LCZX-ray2.33A/B54-65[»]
A/B86-213[»]
5LD0X-ray1.60A86-213[»]
ProteinModelPortaliP04903.
SMRiP04903.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi268945. 1 interactor.
STRINGi10116.ENSRNOP00000043510.

Proteomic databases

PaxDbiP04903.
PRIDEiP04903.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi494499.
KEGGirno:494499.
UCSCiRGD:2754. rat.

Organism-specific databases

CTDi221357.
RGDi2754. Gsta2.

Phylogenomic databases

eggNOGiKOG1695. Eukaryota.
ENOG4111VAU. LUCA.
HOGENOMiHOG000115734.
HOVERGENiHBG053749.
InParanoidiP04903.
KOiK00799.
PhylomeDBiP04903.

Enzyme and pathway databases

BRENDAi2.5.1.18. 5301.

Miscellaneous databases

PROiP04903.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSiPR01266. GSTRNSFRASEA.
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA2_RAT
AccessioniPrimary (citable) accession number: P04903
Secondary accession number(s): Q63715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In addition to its enzymatic activity, the homodimer of Ya chains, called ligandin, binds various organic anions, xenobiotics, and azocarcinogen dyes.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.