Reviewed, UniProtKB/Swiss-Prot P04903 (GSTA2_RAT)

Names and origin

Protein names

Recommended name:
    Glutathione S-transferase alpha-2
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase Ya-2
      Short name=GST Ya2
    GST 1b-1b
    GST A2-2

Gene names

Name:

Gsta2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	In addition to its enzymatic activity, the homodimer of Ya chains, called ligandin, binds various organic anions, xenobiotics, and azocarcinogen dyes.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Biological process	xenobiotic catabolic process Inferred from direct assay. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD nuclear outer membrane Inferred from direct assay. Source: RGD
Molecular function	drug binding Inferred from direct assay. Source: RGD glutathione binding Inferred from direct assay. Source: RGD glutathione transferase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 222	221	Glutathione S-transferase alpha-2		PRO_0000185793
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 208	124	GST C-terminal

Sequences

	Sequence		Length	Mass (Da)
	P04903-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AA342417E9857A9F	FASTA	222	25,559
	10 20 30 40 50 60 MSGKPVLHYF NARGRMECIR WLLAAAGVEF EEKLIQSPED LEKLKKDGNL MFDQVPMVEI 70 80 90 100 110 120 DGMKLAQTRA ILNYIATKYD LYGKDMKERA LIDMYSEGIL DLTEMIIQLV ICPPDQREAK 130 140 150 160 170 180 TALAKDRTKN RYLPAFEKVL KSHGQDYLVG NRLTRVDIHL LELLLYVEEF DASLLTSFPL 190 200 210 220 LKAFKSRISS LPNVKKFLQP GSQRKPAMDA KQIEEARKVF KF

References

[1]	"Rat liver glutathione S-transferases. Complete nucleotide sequence of a glutathione S-transferase mRNA and the regulation of the Ya, Yb, and Yc mRNAs by 3-methylcholanthrene and phenobarbital." Pickett C.B., Telakowski-Hopkins C.A., Ding G.J.-F., Argenbright L., Lu A.Y.H. J. Biol. Chem. 259:5182-5188(1984) [PubMed: 6325423] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Expression and sequence analysis of rat liver glutathione S-transferase genes." Pickett C.B., Telakowsi-Hopkins C.A., Ding G.J.-F., Ding V.D.-H. Adv. Exp. Med. Biol. 197:185-193(1986) [PubMed: 3766257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structural analysis of a rat liver glutathione S-transferase Ya gene." Telakowski-Hopkins C.A., Rothkopf G.S., Pickett C.B. Proc. Natl. Acad. Sci. U.S.A. 83:9393-9397(1986) [PubMed: 3025841] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Construction and characterization of a plasmid containing complementary DNA to mRNA encoding the N-terminal amino acid sequence of the rat glutathione transferase Ya subunit." Taylor J.B., Craig R.K., Beale D., Ketterer B. Biochem. J. 219:223-231(1984) [PubMed: 6547043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-129.
[5]	"Expression of a cDNA encoding a rat liver glutathione S-transferase Ya subunit in Escherichia coli." Wang R.W., Pickett C.B., Lu A.Y.H. Arch. Biochem. Biophys. 269:536-543(1989) [PubMed: 2645828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31. Tissue: Liver.
[6]	"Cloning and sequence analysis of a cDNA plasmid for one of the rat liver glutathione S-transferase subunits." Tu C.-P.D., Weiss M.J., Karakawa W.W., Reddy C.C. Nucleic Acids Res. 10:5407-5419(1982) [PubMed: 6292839] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 81-222.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	K00136 mRNA. Translation: AAA41282.1. M25891 mRNA. Translation: AAA41290.1. M14991 , M14986, M14987, M14988, M14989, M14990 Genomic DNA. Translation: AAA41295.1. X00520 mRNA. Translation: CAA25203.1. M27446 mRNA. Translation: AAA41291.1.
IPI	IPI00555178.
PIR	A24735. A26653. XURTG. A92479.
RefSeq	NP_001010921.1.
UniGene	Rn.40574
3D structure databases
HSSP	HSSP built from PDB template 1F3A based on UniProtKB P13745.
SMR	P04903. Positions 2-221.
ModBase	Search...
Protein-protein interaction databases
STRING	P04903.
Proteomic databases
PRIDE	P04903.
Genome annotation databases
Ensembl	ENSRNOT00000032185; ENSRNOP00000037786; ENSRNOG00000029861; Rattus norvegicus. [Genome view]
GeneID	494499.
KEGG	rno:494499.
UCSC	NM_001010921. rat.
Organism-specific databases
RGD	2754. Gsta2.
Phylogenomic databases
HOVERGEN	P04903.
Enzyme and pathway databases
BRENDA	2.5.1.18. 248.
Gene expression databases
Genevestigator	P04903.
GermOnline	ENSRNOG00000029861. Rattus norvegicus.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11571:SF4. GST_alpha. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	697636.

Entry information

Entry name

GSTA2_RAT

Accession

Primary (citable) accession number: P04903
Secondary accession number(s): Q63715

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)