P04903 (GSTA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase alpha-2
EC=2.5.1.18

Alternative name(s):
GST 1b-1b
GST A2-2
Glutathione S-transferase Ya-2
Short name=GST Ya2

Gene names

Name:

Gsta2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	In addition to its enzymatic activity, the homodimer of Ya chains, called ligandin, binds various organic anions, xenobiotics, and azocarcinogen dyes.
Sequence similarities	Belongs to the GST superfamily. Alpha family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 15967433. Source: RGD xenobiotic catabolic process Inferred from direct assay PubMed 17197701. Source: RGD
Cellular_component	cytosol Inferred from direct assay PubMed 17197701. Source: RGD nuclear outer membrane Inferred from direct assay PubMed 17197701. Source: RGD
Molecular_function	drug binding Inferred from direct assay PubMed 17197701. Source: RGD glutathione binding Inferred from direct assay PubMed 17197701. Source: RGD glutathione transferase activity Inferred from direct assay PubMed 17197701. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 222	221	Glutathione S-transferase alpha-2		PRO_0000185793
Regions
	Domain	3 – 83	81	GST N-terminal
	Domain	85 – 208	124	GST C-terminal
	Region	54 – 55	2	Glutathione binding By similarity
	Region	67 – 68	2	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04903 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AA342417E9857A9F	FASTA	222	25,559
	10 20 30 40 50 60 MSGKPVLHYF NARGRMECIR WLLAAAGVEF EEKLIQSPED LEKLKKDGNL MFDQVPMVEI 70 80 90 100 110 120 DGMKLAQTRA ILNYIATKYD LYGKDMKERA LIDMYSEGIL DLTEMIIQLV ICPPDQREAK 130 140 150 160 170 180 TALAKDRTKN RYLPAFEKVL KSHGQDYLVG NRLTRVDIHL LELLLYVEEF DASLLTSFPL 190 200 210 220 LKAFKSRISS LPNVKKFLQP GSQRKPAMDA KQIEEARKVF KF

References

[1]	"Rat liver glutathione S-transferases. Complete nucleotide sequence of a glutathione S-transferase mRNA and the regulation of the Ya, Yb, and Yc mRNAs by 3-methylcholanthrene and phenobarbital." Pickett C.B., Telakowski-Hopkins C.A., Ding G.J.-F., Argenbright L., Lu A.Y.H. J. Biol. Chem. 259:5182-5188(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Expression and sequence analysis of rat liver glutathione S-transferase genes." Pickett C.B., Telakowsi-Hopkins C.A., Ding G.J.-F., Ding V.D.-H. Adv. Exp. Med. Biol. 197:185-193(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Structural analysis of a rat liver glutathione S-transferase Ya gene." Telakowski-Hopkins C.A., Rothkopf G.S., Pickett C.B. Proc. Natl. Acad. Sci. U.S.A. 83:9393-9397(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Construction and characterization of a plasmid containing complementary DNA to mRNA encoding the N-terminal amino acid sequence of the rat glutathione transferase Ya subunit." Taylor J.B., Craig R.K., Beale D., Ketterer B. Biochem. J. 219:223-231(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-129.
[5]	"Expression of a cDNA encoding a rat liver glutathione S-transferase Ya subunit in Escherichia coli." Wang R.W., Pickett C.B., Lu A.Y.H. Arch. Biochem. Biophys. 269:536-543(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31. Tissue: Liver.
[6]	"Cloning and sequence analysis of a cDNA plasmid for one of the rat liver glutathione S-transferase subunits." Tu C.-P.D., Weiss M.J., Karakawa W.W., Reddy C.C. Nucleic Acids Res. 10:5407-5419(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 81-222.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K00136 mRNA. Translation: AAA41282.1. M25891 mRNA. Translation: AAA41290.1. M14991 , M14986, M14987, M14988, M14989, M14990 Genomic DNA. Translation: AAA41295.1. X00520 mRNA. Translation: CAA25203.1. M27446 mRNA. Translation: AAA41291.1.
IPI	IPI00555178.
PIR	A24735. A26653. XURTG. A92479.
RefSeq	NP_001010921.1. NM_001010921.1.
UniGene	Rn.40574.
3D structure databases
ProteinModelPortal	P04903.
SMR	P04903. Positions 2-221.
ModBase	Search...
Proteomic databases
PaxDb	P04903.
PRIDE	P04903.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	494499.
KEGG	rno:494499.
UCSC	RGD:2754. rat.
Organism-specific databases
CTD	221357.
RGD	2754. Gsta2.
Phylogenomic databases
eggNOG	NOG266414.
HOGENOM	HOG000115734.
HOVERGEN	HBG053749.
InParanoid	P04903.
KO	K00799.
OrthoDB	EOG4XD3S0.
Gene expression databases
ArrayExpress	P04903.
Genevestigator	P04903.
GermOnline	ENSRNOG00000029861. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR003080. GST_alpha. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR11571:SF4. PTHR11571:SF4. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01266. GSTRNSFRASEA.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	697636.

Entry information

Entry name

GSTA2_RAT

Accession

Primary (citable) accession number: P04903
Secondary accession number(s): Q63715

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program