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Reviewed, UniProtKB/Swiss-Prot P04903 (GSTA2_RAT)

Last modified October 13, 2009. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glutathione S-transferase alpha-2
    EC=2.5.1.18
Alternative name(s):
    Glutathione S-transferase Ya-2
      Short name=GST Ya2
    GST 1b-1b
    GST A2-2
Gene names
Name: Gsta2
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length222 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Catalytic activity

RX + glutathione = HX + R-S-glutathione.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Miscellaneous

In addition to its enzymatic activity, the homodimer of Ya chains, called ligandin, binds various organic anions, xenobiotics, and azocarcinogen dyes.

Sequence similarities

Belongs to the GST superfamily. Alpha family.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionTransferase
Gene Ontology (GO)
   Biological processxenobiotic catabolic process

Inferred from direct assay. Source: RGD

   Cellular componentcytosol

Inferred from direct assay. Source: RGD

nuclear outer membrane

Inferred from direct assay. Source: RGD

   Molecular functiondrug binding

Inferred from direct assay. Source: RGD

glutathione binding

Inferred from direct assay. Source: RGD

glutathione transferase activity

Inferred from direct assay. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 222221Glutathione S-transferase alpha-2
PRO_0000185793

Regions

Domain3 – 8381GST N-terminal
Domain85 – 208124GST C-terminal

Sequences

Sequence LengthMass (Da)Tools
P04903-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: AA342417E9857A9F

FASTA22225,559
        10         20         30         40         50         60 
MSGKPVLHYF NARGRMECIR WLLAAAGVEF EEKLIQSPED LEKLKKDGNL MFDQVPMVEI 

        70         80         90        100        110        120 
DGMKLAQTRA ILNYIATKYD LYGKDMKERA LIDMYSEGIL DLTEMIIQLV ICPPDQREAK 

       130        140        150        160        170        180 
TALAKDRTKN RYLPAFEKVL KSHGQDYLVG NRLTRVDIHL LELLLYVEEF DASLLTSFPL 

       190        200        210        220 
LKAFKSRISS LPNVKKFLQP GSQRKPAMDA KQIEEARKVF KF 

« Hide

References

[1]"Rat liver glutathione S-transferases. Complete nucleotide sequence of a glutathione S-transferase mRNA and the regulation of the Ya, Yb, and Yc mRNAs by 3-methylcholanthrene and phenobarbital."
Pickett C.B., Telakowski-Hopkins C.A., Ding G.J.-F., Argenbright L., Lu A.Y.H.
J. Biol. Chem. 259:5182-5188(1984) [PubMed: 6325423] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Expression and sequence analysis of rat liver glutathione S-transferase genes."
Pickett C.B., Telakowsi-Hopkins C.A., Ding G.J.-F., Ding V.D.-H.
Adv. Exp. Med. Biol. 197:185-193(1986) [PubMed: 3766257] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Structural analysis of a rat liver glutathione S-transferase Ya gene."
Telakowski-Hopkins C.A., Rothkopf G.S., Pickett C.B.
Proc. Natl. Acad. Sci. U.S.A. 83:9393-9397(1986) [PubMed: 3025841] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Construction and characterization of a plasmid containing complementary DNA to mRNA encoding the N-terminal amino acid sequence of the rat glutathione transferase Ya subunit."
Taylor J.B., Craig R.K., Beale D., Ketterer B.
Biochem. J. 219:223-231(1984) [PubMed: 6547043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-129.
[5]"Expression of a cDNA encoding a rat liver glutathione S-transferase Ya subunit in Escherichia coli."
Wang R.W., Pickett C.B., Lu A.Y.H.
Arch. Biochem. Biophys. 269:536-543(1989) [PubMed: 2645828] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-31.
Tissue: Liver.
[6]"Cloning and sequence analysis of a cDNA plasmid for one of the rat liver glutathione S-transferase subunits."
Tu C.-P.D., Weiss M.J., Karakawa W.W., Reddy C.C.
Nucleic Acids Res. 10:5407-5419(1982) [PubMed: 6292839] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 81-222.
+Additional computationally mapped references.

Cross-references

Sequence databases

K00136 mRNA. Translation: AAA41282.1.
M25891 mRNA. Translation: AAA41290.1.
M14991 expand/collapse EMBL AC list , M14986, M14987, M14988, M14989, M14990 Genomic DNA. Translation: AAA41295.1.
X00520 mRNA. Translation: CAA25203.1.
M27446 mRNA. Translation: AAA41291.1.
IPIIPI00555178.
PIRA24735.
A26653.
XURTG. A92479.
RefSeqNP_001010921.1.
UniGeneRn.40574

3D structure databases

HSSPHSSP built from PDB template 1F3A based on UniProtKB P13745.
SMRP04903. Positions 2-221.
ModBaseSearch...

Protein-protein interaction databases

STRINGP04903.

Proteomic databases

PRIDEP04903.

Genome annotation databases

EnsemblENSRNOT00000032185; ENSRNOP00000037786; ENSRNOG00000029861; Rattus norvegicus. [Genome view]
GeneID494499.
KEGGrno:494499.
UCSCNM_001010921. rat.

Organism-specific databases

RGD2754. Gsta2.

Phylogenomic databases

HOVERGENP04903.

Enzyme and pathway databases

BRENDA2.5.1.18. 248.

Gene expression databases

GenevestigatorP04903.
GermOnlineENSRNOG00000029861. Rattus norvegicus.

Family and domain databases

InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR003080. GST_alpha.
IPR004046. GST_C.
IPR012335. Thioredoxin_fold.
[Graphical view]
Gene3DG3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHERPTHR11571:SF4. GST_alpha. 1 hit.
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PRINTSPR01266. GSTRNSFRASEA.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio697636.

Entry information

Entry nameGSTA2_RAT
AccessionPrimary (citable) accession number: P04903
Secondary accession number(s): Q63715
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: October 13, 2009
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents