P04876 (MATRX_VSIVG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Matrix protein

Gene names

Name:

M

Organism

Vesicular stomatitis Indiana virus (strain Glasgow) (VSIV) [Complete proteome]

Taxonomic identifier

11278 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Vesiculovirus › Vesicular stomatitis Indiana virus

Virus host

Aedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a major role in assembly and budding of virion. Condensates the ribonucleocapsid core during virus assembly. Shut off cellular transcription by inhibiting mRNA nuclear export through direct interaction with host RAE1-NUP98 complex. This shut off presumably inhibit interferon signaling and thus establishment of antiviral state in virus infected cells. Induces cell-rounding, cytoskeleton disorganization and apoptosis in infected cell By similarity.
Subunit structure	Homomultimer. Interacts with viral nucleocapsid. Interacts with host RAE1-NUP98 complex By similarity.
Subcellular location	Virion membrane; Peripheral membrane protein. Host endomembrane system; Peripheral membrane protein. Host nucleus membrane; Peripheral membrane protein By similarity.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. M contains two overlapping L domains: a PPXY motif which interacts with the WW domain 3 of NEDD4 and a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 By similarity.
Post-translational modification	Phosphorylated by host By similarity.
Biotechnological use	VSV is used as an oncolytic agent for cancer therapy, because of his wide host range, rapid replication and mild pathogenicity in humans. VSV used are mutated at M51R in their matrix protein. These mutated viruses cannot successfully infect normal cells, being unable to counteract the antiviral state induced by interferon-alpha in normal cells. Cancer cells are impeded with responsiveness to interferon, and then can be successfully infected and lysed by the virus.
Miscellaneous	Most abundant protein in the virion By similarity.
Sequence similarities	Belongs to the vesiculoviruses matrix protein family.

Ontologies

Keywords
Biological process	Apoptosis Host-virus interaction Viral budding Viral budding via the host ESCRT complexes Virus exit from host cell
Cellular component	Host membrane Host nucleus Membrane Viral matrix protein Virion
Coding sequence diversity	Alternative initiation
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW viral release from host cell Inferred from electronic annotation. Source: UniProtKB-KW virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell viral envelope Inferred from electronic annotation. Source: InterPro virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative initiation. [Align] [Select]

Isoform M (identifier: P04876-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform M2 (identifier: P04876-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Isoform M3 (identifier: P04876-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Matrix protein		PRO_0000222855
Regions
	Motif	24 – 27	4	PPXY motif
	Motif	37 – 40	4	PTAP/PSAP motif
Natural variations
	Alternative sequence	1 – 50	50	Missing in isoform M3.		VSP_025418
	Alternative sequence	1 – 32	32	Missing in isoform M2.		VSP_025419
Experimental info
	Sequence conflict	32	1	S → N in AAA48444. Ref.2
	Sequence conflict	64	1	S → F in AAA48444. Ref.2
	Sequence conflict	215	1	E → K in AAA48444. Ref.2
	Sequence conflict	228	1	S → F in AAA48444. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform M [UniParc]. Last modified August 13, 1987. Version 1. Checksum: C1DF0CF71510959D	FASTA	237	26,825
	10 20 30 40 50 60 MSSLKKILGL KGKGKKSKKL GIAPPPYEED TSMEYAPSAP IDKSYFGVDE MDTHDPNQLR 70 80 90 100 110 120 YEKSFFTVKM TVRSNRPFRT YSDVAAAVSH WDHMYIGMAG KRPFYKILAF LGSSNLKATP 130 140 150 160 170 180 AVLADQGQPE YHAHCEGRAY LPHRMGKTPP MLNVPEHFRR PFNIGLYKGT IELTMTIYDD 190 200 210 220 230 ESLEAAPMIW DHFNSSKFSD FREKALMFGL IVEEEASGAW VLDSVRHSKW ASLASSF
	Isoform M2 [UniParc]. Checksum: 4A241ED12D67D81F	FASTA	205	23,413
	10 20 30 40 50 60 MEYAPSAPID KSYFGVDEMD THDPNQLRYE KSFFTVKMTV RSNRPFRTYS DVAAAVSHWD 70 80 90 100 110 120 HMYIGMAGKR PFYKILAFLG SSNLKATPAV LADQGQPEYH AHCEGRAYLP HRMGKTPPML 130 140 150 160 170 180 NVPEHFRRPF NIGLYKGTIE LTMTIYDDES LEAAPMIWDH FNSSKFSDFR EKALMFGLIV 190 200 EEEASGAWVL DSVRHSKWAS LASSF
	Isoform M3 [UniParc]. Checksum: 01895063A635E892	FASTA	187	21,411
	10 20 30 40 50 60 MDTHDPNQLR YEKSFFTVKM TVRSNRPFRT YSDVAAAVSH WDHMYIGMAG KRPFYKILAF 70 80 90 100 110 120 LGSSNLKATP AVLADQGQPE YHAHCEGRAY LPHRMGKTPP MLNVPEHFRR PFNIGLYKGT 130 140 150 160 170 180 IELTMTIYDD ESLEAAPMIW DHFNSSKFSD FREKALMFGL IVEEEASGAW VLDSVRHSKW ASLASSF

References

[1]	"Evolution of vesicular stomatitis virus in athymic nude mice: mutations associated with natural killer cell selection." Vandepol S.B., Holland J.J. J. Gen. Virol. 67:441-451(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Sequence alterations in temperature-sensitive M-protein mutants (complementation group III) of vesicular stomatitis virus." Gopalakrishna Y., Lenard J. J. Virol. 56:655-659(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-229.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04452 Genomic RNA. Translation: CAA28051.1. M11754 Genomic RNA. Translation: AAA48444.1.
PIR	MFVNGG. A04112.
3D structure databases
ProteinModelPortal	P04876.
SMR	P04876. Positions 58-227.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.10.460.10. 1 hit.
InterPro	IPR009397. Vesiculo_matrix. [Graphical view]
Pfam	PF06326. Vesiculo_matrix. 1 hit. [Graphical view]
SUPFAM	SSF75404. Vesiculo_matrix. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MATRX_VSIVG

Accession

Primary (citable) accession number: P04876

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program