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Protein

Non-structural protein NS-S

Gene

N

Organism
Bunyavirus La Crosse
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Inhibits host transcriptional machinery, by producing modifications to the phosphorylation state of the C-terminal domain (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II, suggesting that the elongation step of transcription and/or 3'-end processing is prevented. Inhibition of host transcription machinery leads to shut off of host cell protein synthesis and inhibition of the host innate immune response. NSs also seems to be involved in the nuclear relocalization of host PABP1 (By similarity).By similarity

GO - Biological processi

  1. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of eukaryotic host transcription initiation by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein NS-S
Gene namesi
Name:N
OrganismiBunyavirus La Crosse
Taxonomic identifieri11577 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostiCervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
Tamias [TaxID: 13712]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9292Non-structural protein NS-SPRO_0000221973Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR000797. Bunya_NSs.
[Graphical view]
PfamiPF01104. Bunya_NS-S. 1 hit.
[Graphical view]
PIRSFiPIRSF003954. NS-S_OrthobunV. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform NSS (identifier: P04874-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMSHQQVQMD LILMQGIWTS VLKMQNHSTL LQLGSSSSMP QRPRLLSRVS
60 70 80 90
QRGRLTLNLE SGRWRLSIII FLETGTTQLV TTILPSTDYL GI

Note: Produced by alternative initiation in the N gene, but encoded on another frame.

Length:92
Mass (Da):10,441
Last modified:August 12, 1987 - v1
Checksum:i03572181F26C6348
GO
Isoform N (identifier: P04873-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P04873.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:235
Mass (Da):26,530
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401P → L in AAA42780 (PubMed:6684362).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00610 Genomic RNA. Translation: AAA42783.1.
K00108 Genomic RNA. Translation: AAA42780.1.
PIRiA04106. MNVULV.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00610 Genomic RNA. Translation: AAA42783.1.
K00108 Genomic RNA. Translation: AAA42780.1.
PIRiA04106. MNVULV.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000797. Bunya_NSs.
[Graphical view]
PfamiPF01104. Bunya_NS-S. 1 hit.
[Graphical view]
PIRSFiPIRSF003954. NS-S_OrthobunV. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparison of the sequences and coding of La Crosse and snowshoe hare bunyavirus S RNA species."
    Akashi H., Bishop D.H.L.
    J. Virol. 45:1155-1158(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Molecular cloning and sequencing of the La Crosse virus S RNA."
    Cabradilla C.D. Jr., Holloway B.P., Obijeski J.F.
    Virology 128:463-468(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNSS_BUNLC
AccessioniPrimary (citable) accession number: P04874
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 12, 1987
Last sequence update: August 12, 1987
Last modified: September 30, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.