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P04853

- HN_SENDZ

UniProt

P04853 - HN_SENDZ

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Protein

Hemagglutinin-neuraminidase

Gene
HN
Organism
Sendai virus (strain Z) (SeV) (Sendai virus (strain HVJ))
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion.
Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. viral entry into host cell Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

Protein family/group databases

CAZyiGH83. Glycoside Hydrolase Family 83.

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-neuraminidase (EC:3.2.1.18)
Short name:
HN protein
Gene namesi
Name:HN
OrganismiSendai virus (strain Z) (SeV) (Sendai virus (strain HVJ))
Taxonomic identifieri11198 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeRespirovirus
Virus hostiCavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]

Subcellular locationi

Virion membrane; Single-pass type II membrane protein Reviewed prediction. Host cell membrane; Single-pass type II membrane protein Reviewed prediction
Note: Folded in the endoplasmic reticulum By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3737Intravirion Reviewed predictionAdd
BLAST
Transmembranei38 – 5821Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini59 – 575517Virion surface Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi55 – 551C → W: 45% loss of cell surface expression; 88% loss of fusion promotion activity. 1 Publication
Mutagenesisi77 – 771N → G: Loss of glycosylation. 1 Publication
Mutagenesisi448 – 4481N → G: No effect. 1 Publication
Mutagenesisi499 – 4991N → G: Loss of glycosylation; 88% loss of neuraminidase activity. 1 Publication
Mutagenesisi511 – 5111N → G: Loss of glycosylation; 88% loss of neuraminidase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Hemagglutinin-neuraminidasePRO_0000142640Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...); by host2 Publications
Disulfide bondi129 – 129Interchain Reviewed prediction
Glycosylationi499 – 4991N-linked (GlcNAc...); by host2 Publications
Glycosylationi511 – 5111N-linked (GlcNAc...); by host2 Publications

Post-translational modificationi

N-glycosylated; glycans consist of a mixture of high mannose-type oligosaccharides and of complex-type oligosaccharides.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotetramer; composed of disulfide-linked homodimers Inferred. Interacts with F protein trimer.2 Publications

Structurei

3D structure databases

ProteinModelPortaliP04853.
SMRiP04853. Positions 144-572.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 145Incorporation in virion
Regioni59 – 14082Interaction with F proteinAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamiPF00423. HN. 1 hit.
[Graphical view]
PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P04853-1 [UniParc]FASTAAdd to Basket

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MDGDRGKRDS YWSTSPSGST TKPASGWERS SKADTWLLIL SFTQWALSIA    50
TVIICIIISA RQGYSMKEYS MTVEALNMSS REVKESLTSL IRQEVIARAV 100
NIQSSVQTGI PVLLNKNSRD VIQMIDKSCS RQELTQHCES TIAVHHADGI 150
APLEPHSFWR CPVGEPYLSS DPEISLLPGP SLLSGSTTIS GCVRLPSLSI 200
GEAIYAYSSN LITQGCADIG KSYQVLQLGY ISLNSDMFPD LNPVVSHTYD 250
INDNRKSCSV VATGTRGYQL CSMPTVDERT DYSSDGIEDL VLDVLDLKGR 300
TKSHRYRNSE VDLDHPFSAL YPSVGNGIAT EGSLIFLGYG GLTTPLQGDT 350
KCRTQGCQQV SQDTCNEALK ITWLGGKQVV SVIIQVNDYL SERPKIRVTT 400
IPITQNYLGA EGRLLKLGDR VYIYTRSSGW HSQLQIGVLD VSHPLTINWT 450
PHEALSRPGN KECNWYNKCP KECISGVYTD AYPLSPDAAN VATVTLYANT 500
SRVNPTIMYS NTTNIINMLR IKDVQLEAAY TTTSCITHFG KGYCFHIIEI 550
NQKSLNTLQP MLFKTSIPKL CKAES 575
Length:575
Mass (Da):63,410
Last modified:August 13, 1987 - v1
Checksum:i721DC135844636CC
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti23 – 231P → L in strain: wild-type, mutant F1-R, mutant ts-f1 and mutant F1-R / T-5 revertant.
Natural varianti33 – 331A → V in strain: wild-type, mutant F1-R, mutant ts-f1 and mutant F1-R / T-5 revertant.
Natural varianti148 – 1481D → E in strain: wild-type, mutant F1-R, mutant ts-f1 and mutant F1-R / T-5 revertant.
Natural varianti238 – 2381F → I.
Natural varianti405 – 4051Q → E in strain: mutant F1-R, mutant ts-f1 and mutant F1-R / T-5 revertant.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03614 Genomic RNA. Translation: CAA27274.1.
M30202 Genomic RNA. Translation: AAB06282.1.
M30203 Genomic RNA. Translation: AAB06288.1.
M30204 Genomic RNA. Translation: AAB06200.1.
M69046 Genomic RNA. Translation: AAB06294.1.
X02808 mRNA. Translation: CAA26576.1.
PIRiA00878. HNNZSZ.
A24004. HNNZSH.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X03614 Genomic RNA. Translation: CAA27274.1 .
M30202 Genomic RNA. Translation: AAB06282.1 .
M30203 Genomic RNA. Translation: AAB06288.1 .
M30204 Genomic RNA. Translation: AAB06200.1 .
M69046 Genomic RNA. Translation: AAB06294.1 .
X02808 mRNA. Translation: CAA26576.1 .
PIRi A00878. HNNZSZ.
A24004. HNNZSH.

3D structure databases

ProteinModelPortali P04853.
SMRi P04853. Positions 144-572.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view ]
Pfami PF00423. HN. 1 hit.
[Graphical view ]
PIRSFi PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of a full-length cDNA encoding the hemagglutinin-neuraminidase glycoprotein of Sendai virus."
    Miura N., Nakatani Y., Ishiura M., Uchida T., Okada Y.
    FEBS Lett. 188:112-116(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins."
    Shioda T., Iwasaki K., Shibuta H.
    Nucleic Acids Res. 14:1545-1563(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Nucleotide sequence analyses of the genes encoding the HN, M, NP, P, and L proteins of two host range mutants of Sendai virus."
    Middleton Y., Tashiro M., Thai T., Oh J., Seymour J., Pritzer E., Klenk H.-D., Rott R., Seto J.T.
    Virology 176:656-657(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Mutant F1-R and Mutant ts-f1.
  4. "Pneumotropic revertants derived from a pantropic mutant, F1-R, of Sendai virus."
    Tashiro M., James I., Karri S., Wahn K., Tobita K., Klenk H.-D., Rott R., Seto J.T.
    Virology 184:227-234(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Mutant F1-R / T-5 revertant.
  5. "Sendai virus utilizes specific sialyloligosaccharides as host cell receptor determinants."
    Markwell M.A.K., Paulson J.C.
    Proc. Natl. Acad. Sci. U.S.A. 77:5693-5697(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: BINDING TO SIALIC ACID-CONTAINING CELL RECEPTORS.
  6. "Carbohydrate structures of HVJ (Sendai virus) glycoproteins."
    Yoshima H., Nakanishi M., Okada Y., Kobata A.
    J. Biol. Chem. 256:5355-5361(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-77; ASN-499 AND ASN-511.
  7. "Regions on the hemagglutinin-neuraminidase proteins of human parainfluenza virus type-1 and Sendai virus important for membrane fusion."
    Bousse T., Takimoto T., Gorman W.L., Takahashi T., Portner A.
    Virology 204:506-514(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF CYS-55.
  8. "Functional interaction of paramyxovirus glycoproteins: identification of a domain in Sendai virus HN which promotes cell fusion."
    Tanabayashi K., Compans R.W.
    J. Virol. 70:6112-6118(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH F PROTEIN.
  9. "Cytoplasmic domain of Sendai virus HN protein contains a specific sequence required for its incorporation into virions."
    Takimoto T., Bousse T., Coronel E.C., Scroggs R.A., Portner A.
    J. Virol. 72:9747-9754(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INCORPORATION IN THE VIRION.
  10. "Functional analysis of the individual oligosaccharide chains of sendai virus fusion protein."
    Segawa H., Yamashita T., Kawakita M., Taira H.
    J. Biochem. 128:65-72(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT ASN-77; ASN-499 AND ASN-511, MUTAGENESIS OF ASN-77; ASN-448; ASN-499 AND ASN-511.
  11. "Assembly of Sendai virus: M protein interacts with F and HN proteins and with the cytoplasmic tail and transmembrane domain of F protein."
    Ali A., Nayak D.P.
    Virology 276:289-303(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH M PROTEIN.

Entry informationi

Entry nameiHN_SENDZ
AccessioniPrimary (citable) accession number: P04853
Secondary accession number(s): P06863, P27562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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