P04851 (NCAP_MEASE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoprotein

Alternative name(s):
Nucleocapsid protein
Short name=NP
Short name=Protein N

Gene names

Name:	N
Synonyms:	NP

Organism

Measles virus (strain Edmonston) (MeV) (Subacute sclerose panencephalitis virus) [Complete proteome]

Taxonomic identifier

11235 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Morbillivirus › Measles virus

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	523 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest.
Subunit structure	Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, binds the P protein and thereby positions the polymerase on the template. Interacts with human FCGR2B protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm.
Domain	Ncore is globular and carries regions required for N self-assembly and RNA-binding. Ntail is an intrinsically disordered monomeric domain By similarity.
Sequence similarities	Belongs to the paramyxoviruses nucleocapsid family.

Ontologies

Keywords
Biological process	Host-virus interaction
Cellular component	Host cytoplasm Virion
Ligand	RNA-binding Viral nucleoprotein
Molecular function	Ribonucleoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 523	523	Nucleoprotein		PRO_0000142654
Regions
	Region	1 – 400	400	Ncore By similarity
	Region	1 – 375	375	Homomultimerization By similarity
	Region	401 – 523	123	Ntail By similarity
	Region	477 – 505	29	P protein-binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04851 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 51544BF86C349BD6	FASTA	523	58,105
	10 20 30 40 50 60 MATLLRSLAL FKRNKDKPPI TSGSGGAIRG IKHIIIVPIP GDSSITTRSR LLDRLVRLIG 70 80 90 100 110 120 NPDVSGPKLT GALIGILSLF VESPGQLIQR ITDDPDVSIR LLEVVQSDQS QSGLTFASRG 130 140 150 160 170 180 TNMEDEADQY FSHDDPISSD QSRFGWFENK EISDIEVQDP EGFNMILGTI LAQIWVLLAK 190 200 210 220 230 240 AVTAPDTAAD SELRRWIKYT QQRRVVGEFR LERKWLDVVR NIIAEDLSLR RFMVALILDI 250 260 270 280 290 300 KRTPGNKPRI AEMICDIDTY IVEAGLASFI LTIKFGIETM YPALGLHEFA GELSTLESLM 310 320 330 340 350 360 NLYQQMGKPA PYMVNLENSI QNKFSAGSYP LLWSYAMGVG VELENSMGGL NFGRSYFDPA 370 380 390 400 410 420 YFRLGQEMVR RSAGKVSSTL ASELGITAED ARLVSEIAMH TTEDKISRAV GPRQAQVSFL 430 440 450 460 470 480 QGDQSENELP RLGGKEDRRV KQSRGEARES YRETGPSRAS DARAAHLPTG TPLDIDTASE 490 500 510 520 SSQDPQDSRR SAEPLLSCKP WQESRKNKAQ TRTPLQCTMT EIF

References

[1]	"Sequence homology within the morbilliviruses." Rozenblatt S., Eizenberg O., Ben-Levy R., Lavie V., Bellini W.J. J. Virol. 53:684-690(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of DNA corresponding to four different measles virus genomic regions." Billeter M.A., Baczko K., Schmid A., Ter Meulen V. Virology 132:147-159(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-165.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01999 mRNA. Translation: CAA26031.1. K01711 Genomic RNA. Translation: AAA75494.1. Sequence problems.
PIR	VHNZMV. A04027.
3D structure databases
DisProt	DP00160.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002021. Paramyx_ncap. [Graphical view]
Pfam	PF00973. Paramyxo_ncap. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCAP_MEASE

Accession

Primary (citable) accession number: P04851

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program