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P04850 (HN_SV5) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hemagglutinin-neuraminidase
EC=3.2.1.18
Gene names
Name:HN
OrganismSimian virus 5 (strain W3) (SV5) [Complete proteome]
Taxonomic identifier11208 [NCBI]
Taxonomic lineageViruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus ›
Virus hostCanis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]
Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.

Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 565565Hemagglutinin-neuraminidase
PRO_0000142641

Regions

Topological domain1 – 1919Intravirion Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 565525Virion surface Potential

Amino acid modifications

Glycosylation1391N-linked (GlcNAc...); by host
Glycosylation2671N-linked (GlcNAc...); by host
Glycosylation5041N-linked (GlcNAc...); by host
Disulfide bond161 ↔ 185
Disulfide bond175 ↔ 236
Disulfide bond227 ↔ 240
Disulfide bond365 ↔ 375
Disulfide bond448 ↔ 458
Disulfide bond528 ↔ 539

Secondary structure

1 ................................................................................. 565
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P04850 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 30A4A7068A25B563

FASTA56562,205
        10         20         30         40         50         60 
MVAEDAPVRA TCRVLFRTTT LIFLCTLLAL SISILYESLI TQKQIMSQAG STGSNSGLGS 

        70         80         90        100        110        120 
ITDLLNNILS VANQIIYNSA VALPLQLDTL ESTLLTAIKS LQTSDKLEQN CSWSAALIND 

       130        140        150        160        170        180 
NRYINGINQF YFSIAEGRNL TLGPLLNMPS FIPTATTPEG CTRIPSFSLT KTHWCYTHNV 

       190        200        210        220        230        240 
ILNGCQDHVS SNQFVSMGII EPTSAGFPFF RTLKTLYLSD GVNRKSCSIS TVPGGCMMYC 

       250        260        270        280        290        300 
FVSTQPERDD YFSAAPPEQR IIIMYYNDTI VERIINPPGV LDVWATLNPG TGSGVYYLGW 

       310        320        330        340        350        360 
VLFPIYGGVI KGTSLWNNQA NKYFIPQMVA ALCSQNQATQ VQNAKSSYYS SWFGNRMIQS 

       370        380        390        400        410        420 
GILACPLRQD LTNECLVLPF SNDQVLMGAE GRLYMYGDSV YYYQRSNSWW PMTMLYKVTI 

       430        440        450        460        470        480 
TFTNGQPSAI SAQNVPTQQV PRPGTGDCSA TNRCPGFCLT GVYADAWLLT NPSSTSTFGS 

       490        500        510        520        530        540 
EATFTGSYLN TATQRINPTM YIANNTQIIS SQQFGSSGQE AAYGHTTCFR DTGSVMVYCI 

       550        560 
YIIELSSSLL GQFQIVPFIR QVTLS

« Hide

References

[1]"Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor."
Hiebert S.W., Paterson R.G., Lamb R.A.
J. Virol. 54:1-6(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence comparison between the haemagglutinin-neuraminidase genes of simian, canine and human isolates of simian virus 5."
Baty D.U., Southern J.A., Randall R.E.
J. Gen. Virol. 72:3103-3107(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose."
Yuan P., Thompson T.B., Wurzburg B.A., Paterson R.G., Lamb R.A., Jardetzky T.S.
Structure 13:803-815(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 37-565.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S76876 mRNA. Translation: AAB21114.1.
K02870 Genomic RNA. Translation: AAA47878.1.
AF052755 Genomic RNA. Translation: AAC95517.1.
PIRHNNZSV. A00879.
RefSeqYP_138517.1. NC_006430.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Z4VX-ray2.30A37-565[»]
1Z4WX-ray2.70A37-565[»]
1Z4XX-ray2.50A37-565[»]
1Z4YX-ray2.60A37-565[»]
1Z4ZX-ray2.50A37-565[»]
1Z50X-ray2.80A37-565[»]
3TSIX-ray2.65A/B/C/D56-117[»]
ProteinModelPortalP04850.
SMRP04850. Positions 118-565.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-48442N.

Protein family/group databases

CAZyGH83. Glycoside Hydrolase Family 83.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3160803.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR000665. Hemagglutn-neuramid.
IPR016285. Hemagglutn-neuramid_paramyxo.
IPR011040. Sialidases.
[Graphical view]
PfamPF00423. HN. 1 hit.
[Graphical view]
PIRSFPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
SUPFAMSSF50939. Sialidase. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP04850.

Entry information

Entry nameHN_SV5
AccessionPrimary (citable) accession number: P04850
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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