Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P04850

- HN_SV5

UniProt

P04850 - HN_SV5

Protein

Hemagglutinin-neuraminidase

Gene

HN

Organism
Simian virus 5 (strain W3) (SV5)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity.By similarity
    Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins.By similarity

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Keywords - Biological processi

    Host-virus interaction, Viral attachment to host cell, Virus entry into host cell

    Protein family/group databases

    CAZyiGH83. Glycoside Hydrolase Family 83.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-neuraminidase (EC:3.2.1.18)
    Gene namesi
    Name:HN
    OrganismiSimian virus 5 (strain W3) (SV5)
    Taxonomic identifieri11208 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaeParamyxovirinaeRubulavirus
    Virus hostiCanis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]
    Homo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000007232: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 565565Hemagglutinin-neuraminidasePRO_0000142641Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi139 – 1391N-linked (GlcNAc...); by host
    Disulfide bondi161 ↔ 185
    Disulfide bondi175 ↔ 236
    Disulfide bondi227 ↔ 240
    Glycosylationi267 – 2671N-linked (GlcNAc...); by host
    Disulfide bondi365 ↔ 375
    Disulfide bondi448 ↔ 458
    Glycosylationi504 – 5041N-linked (GlcNAc...); by host
    Disulfide bondi528 ↔ 539

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-48442N.

    Structurei

    Secondary structure

    1
    565
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi66 – 694
    Helixi72 – 809
    Helixi82 – 9918
    Turni121 – 1255
    Beta strandi126 – 1294
    Helixi134 – 1374
    Beta strandi140 – 1456
    Beta strandi156 – 1594
    Beta strandi161 – 1699
    Beta strandi174 – 18411
    Beta strandi192 – 20211
    Beta strandi206 – 21813
    Beta strandi224 – 2329
    Beta strandi235 – 2428
    Helixi247 – 2526
    Beta strandi253 – 2553
    Beta strandi258 – 2658
    Beta strandi270 – 2756
    Turni281 – 2833
    Beta strandi284 – 2896
    Beta strandi295 – 2973
    Beta strandi300 – 30910
    Helixi314 – 3196
    Turni330 – 3323
    Helixi337 – 34610
    Turni351 – 3555
    Beta strandi356 – 36712
    Beta strandi376 – 3794
    Turni382 – 3843
    Beta strandi391 – 3966
    Beta strandi399 – 4046
    Beta strandi414 – 43421
    Helixi446 – 4483
    Beta strandi466 – 4705
    Helixi472 – 4743
    Beta strandi475 – 4773
    Turni478 – 4814
    Beta strandi483 – 4897
    Beta strandi491 – 4966
    Beta strandi498 – 5069
    Beta strandi508 – 5136
    Beta strandi520 – 53112
    Turni532 – 5354
    Beta strandi536 – 54611
    Beta strandi548 – 56417

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Z4VX-ray2.30A37-565[»]
    1Z4WX-ray2.70A37-565[»]
    1Z4XX-ray2.50A37-565[»]
    1Z4YX-ray2.60A37-565[»]
    1Z4ZX-ray2.50A37-565[»]
    1Z50X-ray2.80A37-565[»]
    3TSIX-ray2.65A/B/C/D56-117[»]
    4JF7X-ray2.50A/B/C/D56-565[»]
    ProteinModelPortaliP04850.
    SMRiP04850. Positions 118-565.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP04850.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1919IntravirionSequence AnalysisAdd
    BLAST
    Topological domaini41 – 565525Virion surfaceSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei20 – 4021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR000665. Hemagglutn-neuramid.
    IPR016285. Hemagglutn-neuramid_paramyxo.
    IPR011040. Sialidases.
    [Graphical view]
    PfamiPF00423. HN. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P04850-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAEDAPVRA TCRVLFRTTT LIFLCTLLAL SISILYESLI TQKQIMSQAG    50
    STGSNSGLGS ITDLLNNILS VANQIIYNSA VALPLQLDTL ESTLLTAIKS 100
    LQTSDKLEQN CSWSAALIND NRYINGINQF YFSIAEGRNL TLGPLLNMPS 150
    FIPTATTPEG CTRIPSFSLT KTHWCYTHNV ILNGCQDHVS SNQFVSMGII 200
    EPTSAGFPFF RTLKTLYLSD GVNRKSCSIS TVPGGCMMYC FVSTQPERDD 250
    YFSAAPPEQR IIIMYYNDTI VERIINPPGV LDVWATLNPG TGSGVYYLGW 300
    VLFPIYGGVI KGTSLWNNQA NKYFIPQMVA ALCSQNQATQ VQNAKSSYYS 350
    SWFGNRMIQS GILACPLRQD LTNECLVLPF SNDQVLMGAE GRLYMYGDSV 400
    YYYQRSNSWW PMTMLYKVTI TFTNGQPSAI SAQNVPTQQV PRPGTGDCSA 450
    TNRCPGFCLT GVYADAWLLT NPSSTSTFGS EATFTGSYLN TATQRINPTM 500
    YIANNTQIIS SQQFGSSGQE AAYGHTTCFR DTGSVMVYCI YIIELSSSLL 550
    GQFQIVPFIR QVTLS 565
    Length:565
    Mass (Da):62,205
    Last modified:August 13, 1987 - v1
    Checksum:i30A4A7068A25B563
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S76876 mRNA. Translation: AAB21114.1.
    K02870 Genomic RNA. Translation: AAA47878.1.
    AF052755 Genomic RNA. Translation: AAC95517.1.
    PIRiA00879. HNNZSV.
    RefSeqiYP_138517.1. NC_006430.1.

    Genome annotation databases

    GeneIDi3160803.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S76876 mRNA. Translation: AAB21114.1 .
    K02870 Genomic RNA. Translation: AAA47878.1 .
    AF052755 Genomic RNA. Translation: AAC95517.1 .
    PIRi A00879. HNNZSV.
    RefSeqi YP_138517.1. NC_006430.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Z4V X-ray 2.30 A 37-565 [» ]
    1Z4W X-ray 2.70 A 37-565 [» ]
    1Z4X X-ray 2.50 A 37-565 [» ]
    1Z4Y X-ray 2.60 A 37-565 [» ]
    1Z4Z X-ray 2.50 A 37-565 [» ]
    1Z50 X-ray 2.80 A 37-565 [» ]
    3TSI X-ray 2.65 A/B/C/D 56-117 [» ]
    4JF7 X-ray 2.50 A/B/C/D 56-565 [» ]
    ProteinModelPortali P04850.
    SMRi P04850. Positions 118-565.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48442N.

    Protein family/group databases

    CAZyi GH83. Glycoside Hydrolase Family 83.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3160803.

    Miscellaneous databases

    EvolutionaryTracei P04850.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR000665. Hemagglutn-neuramid.
    IPR016285. Hemagglutn-neuramid_paramyxo.
    IPR011040. Sialidases.
    [Graphical view ]
    Pfami PF00423. HN. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor."
      Hiebert S.W., Paterson R.G., Lamb R.A.
      J. Virol. 54:1-6(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence comparison between the haemagglutinin-neuraminidase genes of simian, canine and human isolates of simian virus 5."
      Baty D.U., Southern J.A., Randall R.E.
      J. Gen. Virol. 72:3103-3107(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose."
      Yuan P., Thompson T.B., Wurzburg B.A., Paterson R.G., Lamb R.A., Jardetzky T.S.
      Structure 13:803-815(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 37-565.

    Entry informationi

    Entry nameiHN_SV5
    AccessioniPrimary (citable) accession number: P04850
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3