Reviewed, UniProtKB/Swiss-Prot P04850 (HN_SV5)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Simian virus 5 (strain W3) (SV5)

Taxonomic identifier

11208 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Rubulavirus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Canis familiaris (Dog) [TaxID: 9615]

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Cell membrane Envelope protein Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 565

565

Hemagglutinin-neuraminidase

PRO_0000142641

Regions

Topological domain

1 – 19

19

Cytoplasmic Potential

Transmembrane

20 – 40

21

Potential

Topological domain

41 – 565

525

Extracellular Potential

Amino acid modifications

Glycosylation

139

1

N-linked (GlcNAc...); by host

Glycosylation

267

1

N-linked (GlcNAc...); by host

Glycosylation

504

1

N-linked (GlcNAc...); by host

Disulfide bond

161 ↔ 185

Disulfide bond

175 ↔ 236

Disulfide bond

227 ↔ 240

Disulfide bond

365 ↔ 375

Disulfide bond

448 ↔ 458

Disulfide bond

528 ↔ 539

Secondary structure

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565

Helix Strand Turn

Details...

Turn

121 – 125

5

Beta strand

126 – 129

4

Helix

134 – 137

4

Beta strand

140 – 145

6

Beta strand

156 – 159

4

Beta strand

161 – 169

9

Beta strand

174 – 184

11

Beta strand

192 – 202

11

Beta strand

206 – 218

13

Beta strand

224 – 232

9

Beta strand

235 – 242

8

Helix

247 – 252

6

Beta strand

253 – 255

3

Beta strand

258 – 265

8

Beta strand

270 – 275

6

Turn

281 – 283

3

Beta strand

284 – 289

6

Beta strand

295 – 297

3

Beta strand

300 – 309

10

Helix

314 – 319

6

Turn

330 – 332

3

Helix

337 – 346

10

Turn

351 – 355

5

Beta strand

356 – 367

12

Beta strand

376 – 379

4

Turn

382 – 384

3

Beta strand

391 – 396

6

Beta strand

399 – 404

6

Beta strand

414 – 434

21

Helix

446 – 448

3

Beta strand

466 – 470

5

Helix

472 – 474

3

Beta strand

475 – 477

3

Turn

478 – 481

4

Beta strand

483 – 489

7

Beta strand

491 – 496

6

Beta strand

498 – 506

9

Beta strand

508 – 513

6

Beta strand

520 – 531

12

Turn

532 – 535

4

Beta strand

536 – 546

11

Beta strand

548 – 564

17

Sequences

	Sequence		Length	Mass (Da)
	P04850-1 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 30A4A7068A25B563	FASTA	565	62,205
	10 20 30 40 50 60 MVAEDAPVRA TCRVLFRTTT LIFLCTLLAL SISILYESLI TQKQIMSQAG STGSNSGLGS 70 80 90 100 110 120 ITDLLNNILS VANQIIYNSA VALPLQLDTL ESTLLTAIKS LQTSDKLEQN CSWSAALIND 130 140 150 160 170 180 NRYINGINQF YFSIAEGRNL TLGPLLNMPS FIPTATTPEG CTRIPSFSLT KTHWCYTHNV 190 200 210 220 230 240 ILNGCQDHVS SNQFVSMGII EPTSAGFPFF RTLKTLYLSD GVNRKSCSIS TVPGGCMMYC 250 260 270 280 290 300 FVSTQPERDD YFSAAPPEQR IIIMYYNDTI VERIINPPGV LDVWATLNPG TGSGVYYLGW 310 320 330 340 350 360 VLFPIYGGVI KGTSLWNNQA NKYFIPQMVA ALCSQNQATQ VQNAKSSYYS SWFGNRMIQS 370 380 390 400 410 420 GILACPLRQD LTNECLVLPF SNDQVLMGAE GRLYMYGDSV YYYQRSNSWW PMTMLYKVTI 430 440 450 460 470 480 TFTNGQPSAI SAQNVPTQQV PRPGTGDCSA TNRCPGFCLT GVYADAWLLT NPSSTSTFGS 490 500 510 520 530 540 EATFTGSYLN TATQRINPTM YIANNTQIIS SQQFGSSGQE AAYGHTTCFR DTGSVMVYCI 550 560 YIIELSSSLL GQFQIVPFIR QVTLS

References

[1]	"Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor." Hiebert S.W., Paterson R.G., Lamb R.A. J. Virol. 54:1-6(1985) [PubMed: 3973974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence comparison between the haemagglutinin-neuraminidase genes of simian, canine and human isolates of simian virus 5." Baty D.U., Southern J.A., Randall R.E. J. Gen. Virol. 72:3103-3107(1991) [PubMed: 1765772] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Structural studies of the parainfluenza virus 5 hemagglutinin-neuraminidase tetramer in complex with its receptor, sialyllactose." Yuan P., Thompson T.B., Wurzburg B.A., Paterson R.G., Lamb R.A., Jardetzky T.S. Structure 13:803-815(2005) [PubMed: 15893670] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 37-565.

Cross-references

Sequence databases

EMBLGenBankDDBJ

S76876 mRNA. Translation: AAB21114.1.
K02870 Genomic RNA. Translation: AAA47878.1.
AF052755 Genomic RNA. Translation: AAC95517.1.

PIR

HNNZSV. A00879.

RefSeq

YP_138517.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z4V	X-ray	2.30	A	37-565	[»]
1Z4W	X-ray	2.70	A	37-565	[»]
1Z4X	X-ray	2.50	A	37-565	[»]
1Z4Y	X-ray	2.60	A	37-565	[»]
1Z4Z	X-ray	2.50	A	37-565	[»]
1Z50	X-ray	2.80	A	37-565	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH83. Glycoside Hydrolase Family 83.

Genome annotation databases

GeneID

3160803.

Family and domain databases

InterPro

IPR000665. Hemagglutn-neuramid_glycoprot.
IPR016285. Hemagglutn-neuramid_paramyxo.
[Graphical view]

Pfam

PF00423. HN. 1 hit.
[Graphical view]

PIRSF

PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.

ProtoNet

Search...

Entry information

Entry name

HN_SV5

Accession

Primary (citable) accession number: P04850

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)