P04846 (NLPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein 28

Gene names

Name:	nlpA
Ordered Locus Names:	b3661, JW3635

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell inner membrane; Lipid-anchor.
Sequence similarities	Belongs to the nlpA lipoprotein family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23
	Chain	24 – 272	249	Lipoprotein 28		PRO_0000019737
Amino acid modifications
	Lipidation	24	1	N-palmitoyl cysteine Probable
	Lipidation	24	1	S-diacylglycerol cysteine

Sequences

	Sequence		Length	Mass (Da)
	P04846 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: E3C249E753AB1B33	FASTA	272	29,424
	10 20 30 40 50 60 MKLTTHHLRT GAALLLAGIL LAGCDQSSSD AKHIKVGVIN GAEQDVAEVA KKVAKEKYGL 70 80 90 100 110 120 DVELVGFSGS LLPNDATNHG ELDANVFQHR PFLEQDNQAH GYKLVAVGNT FVFPMAGYSK 130 140 150 160 170 180 KIKTVAQIKE GATVAIPNDP TNLGRALLLL QKEKLITLKE GKGLLPTALD ITDNPRHLQI 190 200 210 220 230 240 MELEGAQLPR VLDDPKVDVA IISTTYIQQT GLSPVHDSVF IEDKNSPYVN ILVAREDNKN 250 260 270 AENVKEFLQS YQSPEVAKAA ETIFNGGAVP GW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lipoprotein-28, a cytoplasmic membrane lipoprotein from Escherichia coli. Cloning, DNA sequence, and expression of its gene." Yu F., Inouye S., Inouye M. J. Biol. Chem. 261:2284-2288(1986) [PubMed: 3003106] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DIACYLGLYCEROL.
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M12163 Genomic DNA. Translation: AAA24080.1. L10328 Genomic DNA. Translation: AAA62013.1. U00096 Genomic DNA. Translation: AAC76684.1. AP009048 Genomic DNA. Translation: BAE77633.1.
PIR	LPEC28. A26286.
RefSeq	NP_418117.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P04846.
SMR	P04846. Positions 28-265.
ModBase	Search...
Protein-protein interaction databases
IntAct	P04846. 7 interactions.
2D gel databases
SWISS-2DPAGE	P04846.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000001091; EBESCP00000001091; EBESCG00000000904. EBESCT00000016419; EBESCP00000015710; EBESCG00000015479.
GeneID	948175.
GenomeReviews	Gene locus JW3635 in contig AP009048_GR. Gene locus b3661 in contig U00096_GR.
KEGG	ecj:JW3635. eco:b3661.
PATRIC	32122811. VBIEscCol129921_3781.
Organism-specific databases
EchoBASE	EB0651.
EcoGene	EG10657. nlpA.
Phylogenomic databases
eggNOG	COG1464.
GeneTree	EBGT00050000009441.
HOGENOM	HBG661773.
OMA	TYLQQTG.
PhylomeDB	P04846.
ProtClustDB	PRK09861.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10657-MONOMER.
Gene expression databases
Genevestigator	P04846.
Family and domain databases
InterPro	IPR004872. Lipoprotein_9. IPR004478. YaeC. [Graphical view]
KO	K02073.
Pfam	PF03180. Lipoprotein_9. 1 hit. [Graphical view]
TIGRFAMs	TIGR00363. TIGR00363. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NLPA_ECOLI

Accession

Primary (citable) accession number: P04846
Secondary accession number(s): Q2M7X3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program