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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2

Gene

RPN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • aging Source: Ensembl
  • cellular protein modification process Source: ProtInc
  • protein N-linked glycosylation Source: GO_Central
  • protein N-linked glycosylation via asparagine Source: HGNC
  • response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciZFISH:HS04244-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-HSA-446203. Asparagine N-linked glycosylation.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (EC:2.4.99.18)
Alternative name(s):
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit
RIBIIR
Ribophorin II
Short name:
RPN-II
Ribophorin-2
Gene namesi
Name:RPN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:10382. RPN2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 540LumenalSequence analysisAdd BLAST518
Transmembranei541 – 561HelicalSequence analysisAdd BLAST21
Topological domaini562 – 571CytoplasmicSequence analysis10
Transmembranei572 – 592HelicalSequence analysisAdd BLAST21
Topological domaini593 – 596LumenalSequence analysis4
Transmembranei597 – 617HelicalSequence analysisAdd BLAST21
Topological domaini618 – 631CytoplasmicSequence analysisAdd BLAST14

GO - Cellular componenti

  • autophagosome membrane Source: Ensembl
  • endoplasmic reticulum Source: HPA
  • endoplasmic reticulum membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • nucleus Source: HPA
  • oligosaccharyltransferase complex Source: HGNC
  • rough endoplasmic reticulum Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi6185.
OpenTargetsiENSG00000118705.
PharmGKBiPA34778.

Polymorphism and mutation databases

BioMutaiRPN2.
DMDMi9297108.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000002224423 – 631Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2Add BLAST609

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi106N-linked (GlcNAc...)1 Publication1
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP04844.
MaxQBiP04844.
PaxDbiP04844.
PeptideAtlasiP04844.
PRIDEiP04844.
TopDownProteomicsiP04844-1. [P04844-1]

PTM databases

iPTMnetiP04844.
PhosphoSitePlusiP04844.
SwissPalmiP04844.

Expressioni

Tissue specificityi

Expressed in all tissues tested.

Gene expression databases

BgeeiENSG00000118705.
CleanExiHS_RPN2.
ExpressionAtlasiP04844. baseline and differential.
GenevisibleiP04844. HS.

Organism-specific databases

HPAiCAB019277.
HPA008297.
HPA025922.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex.3 Publications

Protein-protein interaction databases

BioGridi112100. 92 interactors.
IntActiP04844. 37 interactors.
MINTiMINT-8247596.
STRINGi9606.ENSP00000237530.

Structurei

3D structure databases

ProteinModelPortaliP04844.
SMRiP04844.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SWP1 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2447. Eukaryota.
ENOG410XRF2. LUCA.
GeneTreeiENSGT00390000002635.
HOGENOMiHOG000231261.
HOVERGENiHBG002365.
InParanoidiP04844.
KOiK12667.
OMAiVKKACAF.
OrthoDBiEOG091G0H3U.
PhylomeDBiP04844.
TreeFamiTF106146.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P04844-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPGSSTVF LLALTIIAST WALTPTHYLT KHDVERLKAS LDRPFTNLES
60 70 80 90 100
AFYSIVGLSS LGAQVPDAKK ACTYIRSNLD PSNVDSLFYA AQASQALSGC
110 120 130 140 150
EISISNETKD LLLAAVSEDS SVTQIYHAVA ALSGFGLPLA SQEALSALTA
160 170 180 190 200
RLSKEETVLA TVQALQTASH LSQQADLRSI VEEIEDLVAR LDELGGVYLQ
210 220 230 240 250
FEEGLETTAL FVAATYKLMD HVGTEPSIKE DQVIQLMNAI FSKKNFESLS
260 270 280 290 300
EAFSVASAAA VLSHNRYHVP VVVVPEGSAS DTHEQAILRL QVTNVLSQPL
310 320 330 340 350
TQATVKLEHA KSVASRATVL QKTSFTPVGD VFELNFMNVK FSSGYYDFLV
360 370 380 390 400
EVEGDNRYIA NTVELRVKIS TEVGITNVDL STVDKDQSIA PKTTRVTYPA
410 420 430 440 450
KAKGTFIADS HQNFALFFQL VDVNTGAELT PHQTFVRLHN QKTGQEVVFV
460 470 480 490 500
AEPDNKNVYK FELDTSERKI EFDSASGTYT LYLIIGDATL KNPILWNVAD
510 520 530 540 550
VVIKFPEEEA PSTVLSQNLF TPKQEIQHLF REPEKRPPTV VSNTFTALIL
560 570 580 590 600
SPLLLLFALW IRIGANVSNF TFAPSTIIFH LGHAAMLGLM YVYWTQLNMF
610 620 630
QTLKYLAILG SVTFLAGNRM LAQQAVKRTA H
Length:631
Mass (Da):69,284
Last modified:December 1, 2000 - v3
Checksum:iE24D7B3565141676
GO
Isoform 2 (identifier: P04844-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     70-101: Missing.
     627-627: K → KRIAAEQSSRLAKYRTL

Note: No experimental confirmation available.
Show »
Length:615
Mass (Da):67,723
Checksum:iFC967C1FA17A011E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197V → L in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti201F → C in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti260A → S in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti286A → S in CAG33180 (Ref. 4) Curated1
Sequence conflicti423V → M in CAA68393 (PubMed:3034581).Curated1
Sequence conflicti427A → V in CAG33180 (Ref. 4) Curated1
Sequence conflicti571T → I in AAH13028 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_054040597L → F.Corresponds to variant rs34951322dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04305170 – 101Missing in isoform 2. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_043052627K → KRIAAEQSSRLAKYRTL in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00282 mRNA. Translation: CAA68393.1.
AJ237734
, AJ237735, AJ237733, AJ237736, AJ237737, AJ237738, AJ237739, AJ237740, AJ237741, AJ237742, AJ237743, AJ237744, AJ237745, AJ237746, AJ237747, AJ237748, AJ237749 Genomic DNA. Translation: CAB54801.1.
AK096243 mRNA. Translation: BAG53237.1.
CR456899 mRNA. Translation: CAG33180.1.
AL031659 Genomic DNA. Translation: CAB41763.1.
AL031659 Genomic DNA. Translation: CAI42668.1.
CH471077 Genomic DNA. Translation: EAW76073.1.
BC002380 mRNA. Translation: AAH02380.2.
BC003560 mRNA. Translation: AAH03560.1.
BC013028 mRNA. Translation: AAH13028.2.
BC020222 mRNA. Translation: AAH20222.1.
CCDSiCCDS13291.1. [P04844-1]
CCDS46599.1. [P04844-2]
PIRiB26168.
RefSeqiNP_001129243.1. NM_001135771.2. [P04844-2]
NP_001311228.1. NM_001324299.1.
NP_001311230.1. NM_001324301.1.
NP_001311231.1. NM_001324302.1.
NP_001311232.1. NM_001324303.1.
NP_001311233.1. NM_001324304.1.
NP_001311234.1. NM_001324305.1.
NP_001311235.1. NM_001324306.1.
NP_002942.2. NM_002951.4. [P04844-1]
UniGeneiHs.370895.

Genome annotation databases

EnsembliENST00000237530; ENSP00000237530; ENSG00000118705. [P04844-1]
ENST00000373622; ENSP00000362724; ENSG00000118705. [P04844-2]
GeneIDi6185.
KEGGihsa:6185.
UCSCiuc002xgp.4. human. [P04844-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00282 mRNA. Translation: CAA68393.1.
AJ237734
, AJ237735, AJ237733, AJ237736, AJ237737, AJ237738, AJ237739, AJ237740, AJ237741, AJ237742, AJ237743, AJ237744, AJ237745, AJ237746, AJ237747, AJ237748, AJ237749 Genomic DNA. Translation: CAB54801.1.
AK096243 mRNA. Translation: BAG53237.1.
CR456899 mRNA. Translation: CAG33180.1.
AL031659 Genomic DNA. Translation: CAB41763.1.
AL031659 Genomic DNA. Translation: CAI42668.1.
CH471077 Genomic DNA. Translation: EAW76073.1.
BC002380 mRNA. Translation: AAH02380.2.
BC003560 mRNA. Translation: AAH03560.1.
BC013028 mRNA. Translation: AAH13028.2.
BC020222 mRNA. Translation: AAH20222.1.
CCDSiCCDS13291.1. [P04844-1]
CCDS46599.1. [P04844-2]
PIRiB26168.
RefSeqiNP_001129243.1. NM_001135771.2. [P04844-2]
NP_001311228.1. NM_001324299.1.
NP_001311230.1. NM_001324301.1.
NP_001311231.1. NM_001324302.1.
NP_001311232.1. NM_001324303.1.
NP_001311233.1. NM_001324304.1.
NP_001311234.1. NM_001324305.1.
NP_001311235.1. NM_001324306.1.
NP_002942.2. NM_002951.4. [P04844-1]
UniGeneiHs.370895.

3D structure databases

ProteinModelPortaliP04844.
SMRiP04844.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112100. 92 interactors.
IntActiP04844. 37 interactors.
MINTiMINT-8247596.
STRINGi9606.ENSP00000237530.

PTM databases

iPTMnetiP04844.
PhosphoSitePlusiP04844.
SwissPalmiP04844.

Polymorphism and mutation databases

BioMutaiRPN2.
DMDMi9297108.

Proteomic databases

EPDiP04844.
MaxQBiP04844.
PaxDbiP04844.
PeptideAtlasiP04844.
PRIDEiP04844.
TopDownProteomicsiP04844-1. [P04844-1]

Protocols and materials databases

DNASUi6185.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000237530; ENSP00000237530; ENSG00000118705. [P04844-1]
ENST00000373622; ENSP00000362724; ENSG00000118705. [P04844-2]
GeneIDi6185.
KEGGihsa:6185.
UCSCiuc002xgp.4. human. [P04844-1]

Organism-specific databases

CTDi6185.
DisGeNETi6185.
GeneCardsiRPN2.
HGNCiHGNC:10382. RPN2.
HPAiCAB019277.
HPA008297.
HPA025922.
MIMi180490. gene.
neXtProtiNX_P04844.
OpenTargetsiENSG00000118705.
PharmGKBiPA34778.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2447. Eukaryota.
ENOG410XRF2. LUCA.
GeneTreeiENSGT00390000002635.
HOGENOMiHOG000231261.
HOVERGENiHBG002365.
InParanoidiP04844.
KOiK12667.
OMAiVKKACAF.
OrthoDBiEOG091G0H3U.
PhylomeDBiP04844.
TreeFamiTF106146.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciZFISH:HS04244-MONOMER.
ReactomeiR-HSA-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-HSA-446203. Asparagine N-linked glycosylation.

Miscellaneous databases

ChiTaRSiRPN2. human.
GeneWikiiRPN2.
GenomeRNAii6185.
PROiP04844.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000118705.
CleanExiHS_RPN2.
ExpressionAtlasiP04844. baseline and differential.
GenevisibleiP04844. HS.

Family and domain databases

InterProiIPR008814. Swp1.
[Graphical view]
PANTHERiPTHR12640:SF0. PTHR12640:SF0. 1 hit.
PfamiPF05817. Ribophorin_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPN2_HUMAN
AccessioniPrimary (citable) accession number: P04844
Secondary accession number(s): Q5JYR6
, Q6IBA5, Q96E21, Q9BUQ3, Q9UBE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 168 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.