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Protein

Alcohol oxidase 1

Gene

AOX1

Organism
Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.1 Publication

Catalytic activityi

A primary alcohol + O2 = an aldehyde + H2O2.

Cofactori

Pathwayi: methane degradation

This protein is involved in the pathway methane degradation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway methane degradation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei567Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 38FADBy similarityAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Methanol utilization

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00147.

Protein family/group databases

CAZyiAA3. Auxiliary Activities 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol oxidase 1 (EC:1.1.3.13)
Short name:
AO 1
Short name:
AOX 1
Alternative name(s):
Methanol oxidase 1
Short name:
MOX 1
Gene namesi
Name:AOX1
Ordered Locus Names:PAS_chr4_0821
OrganismiKomagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
Proteomesi
  • UP000000314 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002055822 – 663Alcohol oxidase 1Add BLAST662

Proteomic databases

PRIDEiP04842.

Expressioni

Inductioni

Induced by methanol. Subject to strong carbon catabolite repression.1 Publication

Interactioni

Subunit structurei

Homooctamer. Assembles only after import into the peroxisomal matrix, fails to assemble in the cytoplasm.1 Publication

Protein-protein interaction databases

STRINGi644223.XP_002494271.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I68electron microscopy3.37A1-663[»]
ProteinModelPortaliP04842.
SMRiP04842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi661 – 663Microbody targeting signal3

Domaini

The C-terminal peroxisomal targeting signal (PTS) is essential for the efficient targeting and import of AOX into peroxisomes via the PTS1 pathway.1 Publication

Sequence similaritiesi

Belongs to the GMC oxidoreductase family.Curated

Phylogenomic databases

eggNOGiKOG1238. Eukaryota.
COG2303. LUCA.
HOGENOMiHOG000139601.
KOiK17066.
OMAiSHASEVW.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04842-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPEEFDIL VLGGGSSGSC IAGRLANLDH SLKVGLIEAG ENNLNNPWVY
60 70 80 90 100
LPGIYPRNMK LDSKTASFYT SNPSPHLNGR RAIVPCANVL GGGSSINFMM
110 120 130 140 150
YTRGSASDYD DFQAEGWKTK DLLPLMKKTE TYQRACNNPD IHGFEGPIKV
160 170 180 190 200
SFGNYTYPVC QDFLRASESQ GIPYVDDLED LVTAHGAEHW LKWINRDTGR
210 220 230 240 250
RSDSAHAFVH STMRNHDNLY LICNTKVDKI IVEDGRAAAV RTVPSKPLNP
260 270 280 290 300
KKPSHKIYRA RKQIVLSCGT ISSPLVLQRS GFGDPIKLRA AGVKPLVNLP
310 320 330 340 350
GVGRNFQDHY CFFSPYRIKP QYESFDDFVR GDAEIQKRVF DQWYANGTGP
360 370 380 390 400
LATNGIEAGV KIRPTPEELS QMDESFQEGY REYFEDKPDK PVMHYSIIAG
410 420 430 440 450
FFGDHTKIPP GKYMTMFHFL EYPFSRGSIH ITSPDPYAAP DFDPGFMNDE
460 470 480 490 500
RDMAPMVWAY KKSRETARRM DHFAGEVTSH HPLFPYSSEA RALEMDLETS
510 520 530 540 550
NAYGGPLNLS AGLAHGSWTQ PLKKPTAKNE GHVTSNQVEL HPDIEYDEED
560 570 580 590 600
DKAIENYIRE HTETTWHCLG TCSIGPREGS KIVKWGGVLD HRSNVYGVKG
610 620 630 640 650
LKVGDLSVCP DNVGCNTYTT ALLIGEKTAT LVGEDLGYSG EALDMTVPQF
660
KLGTYEKTGL ARF
Length:663
Mass (Da):73,898
Last modified:March 19, 2014 - v2
Checksum:i2206F9319CDC96ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02646 Genomic DNA. Translation: CAA26484.1.
FN392322 Genomic DNA. Translation: CAY72092.1.
PIRiA23483.
RefSeqiXP_002494271.1. XM_002494226.1.

Genome annotation databases

EnsemblFungiiCAY72092; CAY72092; PAS_chr4_0821.
GeneIDi8201223.
KEGGippa:PAS_chr4_0821.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02646 Genomic DNA. Translation: CAA26484.1.
FN392322 Genomic DNA. Translation: CAY72092.1.
PIRiA23483.
RefSeqiXP_002494271.1. XM_002494226.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I68electron microscopy3.37A1-663[»]
ProteinModelPortaliP04842.
SMRiP04842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi644223.XP_002494271.1.

Protein family/group databases

CAZyiAA3. Auxiliary Activities 3.

Proteomic databases

PRIDEiP04842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAY72092; CAY72092; PAS_chr4_0821.
GeneIDi8201223.
KEGGippa:PAS_chr4_0821.

Phylogenomic databases

eggNOGiKOG1238. Eukaryota.
COG2303. LUCA.
HOGENOMiHOG000139601.
KOiK17066.
OMAiSHASEVW.

Enzyme and pathway databases

UniPathwayiUPA00147.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALOX1_KOMPG
AccessioniPrimary (citable) accession number: P04842
Secondary accession number(s): C4R917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: March 19, 2014
Last modified: November 2, 2016
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.