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Protein

Alcohol oxidase 1

Gene

AOX1

Organism
Komagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.1 Publication

Catalytic activityi

A primary alcohol + O2 = an aldehyde + H2O2.

Cofactori

Pathwayi: methane degradation

This protein is involved in the pathway methane degradation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway methane degradation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei567Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 38FADBy similarityAdd BLAST31

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processMethanol utilization
LigandFAD, Flavoprotein

Enzyme and pathway databases

UniPathwayiUPA00147.

Protein family/group databases

CAZyiAA3. Auxiliary Activities 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol oxidase 1 (EC:1.1.3.13)
Short name:
AO 1
Short name:
AOX 1
Alternative name(s):
Methanol oxidase 1
Short name:
MOX 1
Gene namesi
Name:AOX1
Ordered Locus Names:PAS_chr4_0821
OrganismiKomagataella phaffii (strain GS115 / ATCC 20864) (Yeast) (Pichia pastoris)
Taxonomic identifieri644223 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
Proteomesi
  • UP000000314 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002055822 – 663Alcohol oxidase 1Add BLAST662

Proteomic databases

PRIDEiP04842.

Expressioni

Inductioni

Induced by methanol. Subject to strong carbon catabolite repression.1 Publication

Interactioni

Subunit structurei

Homooctamer. Assembles only after import into the peroxisomal matrix, fails to assemble in the cytoplasm.1 Publication

Protein-protein interaction databases

STRINGi644223.XP_002494271.1.

Structurei

Secondary structure

1663
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 12Combined sources8
Helixi16 – 29Combined sources14
Beta strandi30 – 32Combined sources3
Beta strandi34 – 37Combined sources4
Helixi47 – 50Combined sources4
Helixi52 – 54Combined sources3
Helixi56 – 59Combined sources4
Helixi75 – 77Combined sources3
Helixi92 – 95Combined sources4
Helixi106 – 111Combined sources6
Helixi119 – 127Combined sources9
Beta strandi129 – 132Combined sources4
Turni139 – 141Combined sources3
Beta strandi147 – 151Combined sources5
Helixi159 – 168Combined sources10
Turni169 – 171Combined sources3
Beta strandi180 – 182Combined sources3
Beta strandi192 – 194Combined sources3
Turni196 – 198Combined sources3
Helixi204 – 208Combined sources5
Helixi210 – 215Combined sources6
Beta strandi217 – 222Combined sources6
Beta strandi224 – 227Combined sources4
Beta strandi239 – 246Combined sources8
Beta strandi256 – 266Combined sources11
Helixi269 – 279Combined sources11
Helixi285 – 289Combined sources5
Turni290 – 292Combined sources3
Turni300 – 303Combined sources4
Beta strandi304 – 307Combined sources4
Beta strandi310 – 318Combined sources9
Helixi325 – 327Combined sources3
Turni328 – 331Combined sources4
Helixi335 – 345Combined sources11
Helixi350 – 352Combined sources3
Beta strandi355 – 362Combined sources8
Helixi366 – 369Combined sources4
Helixi374 – 383Combined sources10
Beta strandi392 – 400Combined sources9
Helixi405 – 407Combined sources3
Beta strandi413 – 422Combined sources10
Beta strandi427 – 430Combined sources4
Beta strandi433 – 435Combined sources3
Beta strandi441 – 443Combined sources3
Helixi450 – 452Combined sources3
Helixi454 – 467Combined sources14
Beta strandi468 – 470Combined sources3
Beta strandi473 – 476Combined sources4
Helixi478 – 480Combined sources3
Helixi497 – 504Combined sources8
Turni534 – 536Combined sources3
Helixi548 – 561Combined sources14
Beta strandi581 – 583Combined sources3
Beta strandi593 – 595Combined sources3
Beta strandi599 – 603Combined sources5
Helixi606 – 608Combined sources3
Helixi618 – 635Combined sources18
Turni655 – 657Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I68electron microscopy3.37A1-663[»]
ProteinModelPortaliP04842.
SMRiP04842.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi661 – 663Microbody targeting signal3

Domaini

The C-terminal peroxisomal targeting signal (PTS) is essential for the efficient targeting and import of AOX into peroxisomes via the PTS1 pathway.1 Publication

Sequence similaritiesi

Belongs to the GMC oxidoreductase family.Curated

Phylogenomic databases

eggNOGiKOG1238. Eukaryota.
COG2303. LUCA.
HOGENOMiHOG000139601.
KOiK17066.
OMAiSHASEVW.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
PfamiView protein in Pfam
PF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiView protein in PROSITE
PS00623. GMC_OXRED_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04842-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPEEFDIL VLGGGSSGSC IAGRLANLDH SLKVGLIEAG ENNLNNPWVY
60 70 80 90 100
LPGIYPRNMK LDSKTASFYT SNPSPHLNGR RAIVPCANVL GGGSSINFMM
110 120 130 140 150
YTRGSASDYD DFQAEGWKTK DLLPLMKKTE TYQRACNNPD IHGFEGPIKV
160 170 180 190 200
SFGNYTYPVC QDFLRASESQ GIPYVDDLED LVTAHGAEHW LKWINRDTGR
210 220 230 240 250
RSDSAHAFVH STMRNHDNLY LICNTKVDKI IVEDGRAAAV RTVPSKPLNP
260 270 280 290 300
KKPSHKIYRA RKQIVLSCGT ISSPLVLQRS GFGDPIKLRA AGVKPLVNLP
310 320 330 340 350
GVGRNFQDHY CFFSPYRIKP QYESFDDFVR GDAEIQKRVF DQWYANGTGP
360 370 380 390 400
LATNGIEAGV KIRPTPEELS QMDESFQEGY REYFEDKPDK PVMHYSIIAG
410 420 430 440 450
FFGDHTKIPP GKYMTMFHFL EYPFSRGSIH ITSPDPYAAP DFDPGFMNDE
460 470 480 490 500
RDMAPMVWAY KKSRETARRM DHFAGEVTSH HPLFPYSSEA RALEMDLETS
510 520 530 540 550
NAYGGPLNLS AGLAHGSWTQ PLKKPTAKNE GHVTSNQVEL HPDIEYDEED
560 570 580 590 600
DKAIENYIRE HTETTWHCLG TCSIGPREGS KIVKWGGVLD HRSNVYGVKG
610 620 630 640 650
LKVGDLSVCP DNVGCNTYTT ALLIGEKTAT LVGEDLGYSG EALDMTVPQF
660
KLGTYEKTGL ARF
Length:663
Mass (Da):73,898
Last modified:March 19, 2014 - v2
Checksum:i2206F9319CDC96ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02646 Genomic DNA. Translation: CAA26484.1.
FN392322 Genomic DNA. Translation: CAY72092.1.
PIRiA23483.
RefSeqiXP_002494271.1. XM_002494226.1.

Genome annotation databases

EnsemblFungiiCAY72092; CAY72092; PAS_chr4_0821.
GeneIDi8201223.
KEGGippa:PAS_chr4_0821.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02646 Genomic DNA. Translation: CAA26484.1.
FN392322 Genomic DNA. Translation: CAY72092.1.
PIRiA23483.
RefSeqiXP_002494271.1. XM_002494226.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5I68electron microscopy3.37A1-663[»]
ProteinModelPortaliP04842.
SMRiP04842.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi644223.XP_002494271.1.

Protein family/group databases

CAZyiAA3. Auxiliary Activities 3.

Proteomic databases

PRIDEiP04842.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAY72092; CAY72092; PAS_chr4_0821.
GeneIDi8201223.
KEGGippa:PAS_chr4_0821.

Phylogenomic databases

eggNOGiKOG1238. Eukaryota.
COG2303. LUCA.
HOGENOMiHOG000139601.
KOiK17066.
OMAiSHASEVW.

Enzyme and pathway databases

UniPathwayiUPA00147.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
PfamiView protein in Pfam
PF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiView protein in PROSITE
PS00623. GMC_OXRED_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiALOX1_KOMPG
AccessioniPrimary (citable) accession number: P04842
Secondary accession number(s): C4R917
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: March 19, 2014
Last modified: February 15, 2017
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.