P04841 (ALOX_PICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alcohol oxidase
Short name=AOX
EC=1.1.3.13

Alternative name(s):
Methanol oxidase
Short name=MOX

Gene names

Name:

MOX

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

870730 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetales incertae sedis › Ogataea

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A primary alcohol + O2 = an aldehyde + H2O2.
Cofactor	FAD.
Pathway	Energy metabolism; methane degradation.
Subunit structure	Homooctamer.
Subcellular location	Peroxisome.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Biological process	Methanol utilization
Cellular component	Peroxisome
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	methane catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway methanol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alcohol oxidase activity Inferred from electronic annotation. Source: EC choline dehydrogenase activity Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 664	664	Alcohol oxidase		PRO_0000205581
Regions
	Nucleotide binding	8 – 39	32	FAD Probable
	Motif	662 – 664	3	Microbody targeting signal Potential
Sites
	Active site	568	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P04841 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 6A4E3AFD6688BD5D	FASTA	664	74,089
	10 20 30 40 50 60 MAIPDEFDII VVGGGSTGCC IAGRLANLDD QNLTVALIEG GENNINNPWV YLPGVYPRNM 70 80 90 100 110 120 RLDSKTATFY SSRPSKALNG RRAIVPCANI LGGGSSINFL MYTRASASDY DDWESEGWST 130 140 150 160 170 180 DELLPLIKKI ETYQRPCNNR DLHGFDGPIK VSFGNYTYPT CQDFLRAAES QGIPVVDDLE 190 200 210 220 230 240 DFKTSHGAEH WLKWINRDLG RRSDSAHAYV HPTMRNKQSL FLITSTKCDK VIIEDGKAVA 250 260 270 280 290 300 VRTVPMKPLN PKKPVSRTFR ARKQIVISCG TISSPLVLQR SGIGAAHHLR SVGVKPIVDL 310 320 330 340 350 360 PGVGENFQDH YCFFTPYYVK PDVPTFDDFV RGDPVAQKAA FDQWYSNKDG PLTTNGIEAG 370 380 390 400 410 420 VKIRPTEEEL ATADEDFRRG YAEYFENKPD KPLMHYSVIS GFFGDHTKIP NGKFMTMFHF 430 440 450 460 470 480 LEYPFSRGFV RITSANPYDA PDFDPGFLND ERDLWPMVWA YKKSRETARR MESFAGEVTS 490 500 510 520 530 540 HHPLFKVDSP ARARDLDLET CSAYAGPKHL TANLYHGSWT VPIDKPTPKN DFHVTSNQVQ 550 560 570 580 590 600 LHSDIEYTEE DDEAIVNYIK EHTETTWHCL GTCSMAPREG SKIAPKGGVL DARLNVYGVQ 610 620 630 640 650 660 NLKVADLSVC PDNVGCNTYS TALTIGEKAA TLVAEDLGYS GSDLDMTIPN FRLGTYEETG LARF

References

[1]	"Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha." Ledeboer A.M., Edens L., Maat J., Visser C., Bos J.W., Verrips C.T. Nucleic Acids Res. 13:3063-3082(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL Y-5445.
[2]	"Drosophila glucose dehydrogenase and yeast alcohol oxidase are homologous and share N-terminal homology with other flavoenzymes." Cavener D.R., Krasney P. Mol. Biol. Evol. 8:144-150(1991) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO DROSOPHILA GLUCOSE DEHYDROGENASE.
[3]	"Biosynthesis and assembly of alcohol oxidase, a peroxisomal matrix protein in methylotrophic yeasts: a review." van der Klei I.J., Harder W., Veenhuis M. Yeast 7:195-209(1991) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02425 Genomic DNA. Translation: CAA26278.1.
PIR	OXHQAP. A23010.
3D structure databases
ProteinModelPortal	P04841.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00147.
Family and domain databases
InterPro	IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALOX_PICAN

Accession

Primary (citable) accession number: P04841

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program