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Protein

Mitochondrial outer membrane protein porin 1

Gene

POR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a channel through the cell membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Is the major permeability fator of the mitochondrial outer membrane.1 Publication

GO - Molecular functioni

  1. porin activity Source: UniProtKB-KW
  2. voltage-gated anion channel activity Source: SGD

GO - Biological processi

  1. apoptotic process Source: SGD
  2. cell redox homeostasis Source: SGD
  3. DNA transport Source: SGD
  4. ion transport Source: SGD
  5. mitochondrion organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33087-MONOMER.
ReactomeiREACT_334110. Mitochondrial protein import.

Protein family/group databases

TCDBi1.B.8.1.1. the mitochondrial and plastid porin (mpp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial outer membrane protein porin 1
Alternative name(s):
Voltage-dependent anion-selective channel protein 1
Short name:
VDAC-1
Gene namesi
Name:POR1
Synonyms:OMP2, VDAC1
Ordered Locus Names:YNL055C
ORF Names:N2441, YNL2441C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIV

Organism-specific databases

SGDiS000005000. POR1.

Subcellular locationi

GO - Cellular componenti

  1. integral component of mitochondrial outer membrane Source: SGD
  2. mitochondrial intermembrane space Source: Reactome
  3. mitochondrial outer membrane Source: SGD
  4. mitochondrion Source: SGD
  5. pore complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 191K → E: 2.5-fold reduction in steepness of voltage dependence. 1 Publication
Mutagenesisi51 – 511D → K: 2-fold increase in steepness of voltage dependence. 1 Publication
Mutagenesisi61 – 611K → E: Alters the selectivity of VDAC. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 283282Mitochondrial outer membrane protein porin 1PRO_0000050524Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei109 – 1091Phosphoserine2 Publications
Modified residuei117 – 1171Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP04840.
PaxDbiP04840.
PeptideAtlasiP04840.

2D gel databases

UCD-2DPAGEP04840.

Expressioni

Gene expression databases

GenevestigatoriP04840.

Interactioni

Subunit structurei

Interacts with AIM5, FCJ1 and MOS1.1 Publication

Protein-protein interaction databases

BioGridi35766. 191 interactions.
DIPiDIP-6453N.
IntActiP04840. 70 interactions.
MINTiMINT-677066.
STRINGi4932.YNL055C.

Structurei

3D structure databases

ProteinModelPortaliP04840.
SMRiP04840. Positions 3-283.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists mainly of membrane-spanning sided beta-sheets. 19 strands distributed along the entire VDAC protein, have the potential to adopt transmembrane beta pleated sheet structures, which rolled together may form a "beta-barrel" type structure, possessing pore dimensions.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiNOG243169.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
InParanoidiP04840.
KOiK15040.
OMAiKSTHEGA.
OrthoDBiEOG7GXPNQ.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
[Graphical view]
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPPVYSDIS RNINDLLNKD FYHATPAAFD VQTTTANGIK FSLKAKQPVK
60 70 80 90 100
DGPLSTNVEA KLNDKQTGLG LTQGWSNTNN LQTKLEFANL TPGLKNELIT
110 120 130 140 150
SLTPGVAKSA VLNTTFTQPF FTARGAFDLC LKSPTFVGDL TMAHEGIVGG
160 170 180 190 200
AEFGYDISAG SISRYAMALS YFAKDYSLGA TLNNEQITTV DFFQNVNAFL
210 220 230 240 250
QVGAKATMNC KLPNSNVNIE FATRYLPDAS SQVKAKVSDS GIVTLAYKQL
260 270 280
LRPGVTLGVG SSFDALKLSE PVHKLGWSLS FDA
Length:283
Mass (Da):30,428
Last modified:January 22, 2007 - v4
Checksum:iC99D03711AF9B54D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti118 – 1181Q → E (PubMed:2408884).Curated
Sequence conflicti118 – 1181Q → E (PubMed:2442148).Curated
Sequence conflicti203 – 2075GAKAT → AKATM in AAA35208 (PubMed:2442148).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02324 mRNA. Translation: CAA26184.1.
M34907 mRNA. Translation: AAA35208.1.
U12141 Genomic DNA. Translation: AAA99656.1.
Z71331 Genomic DNA. Translation: CAA95926.1.
BK006947 Genomic DNA. Translation: DAA10489.1.
PIRiS58721. MMBYP.
RefSeqiNP_014343.1. NM_001182894.1.

Genome annotation databases

EnsemblFungiiYNL055C; YNL055C; YNL055C.
GeneIDi855669.
KEGGisce:YNL055C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02324 mRNA. Translation: CAA26184.1.
M34907 mRNA. Translation: AAA35208.1.
U12141 Genomic DNA. Translation: AAA99656.1.
Z71331 Genomic DNA. Translation: CAA95926.1.
BK006947 Genomic DNA. Translation: DAA10489.1.
PIRiS58721. MMBYP.
RefSeqiNP_014343.1. NM_001182894.1.

3D structure databases

ProteinModelPortaliP04840.
SMRiP04840. Positions 3-283.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35766. 191 interactions.
DIPiDIP-6453N.
IntActiP04840. 70 interactions.
MINTiMINT-677066.
STRINGi4932.YNL055C.

Protein family/group databases

TCDBi1.B.8.1.1. the mitochondrial and plastid porin (mpp) family.

2D gel databases

UCD-2DPAGEP04840.

Proteomic databases

MaxQBiP04840.
PaxDbiP04840.
PeptideAtlasiP04840.

Protocols and materials databases

DNASUi855669.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL055C; YNL055C; YNL055C.
GeneIDi855669.
KEGGisce:YNL055C.

Organism-specific databases

SGDiS000005000. POR1.

Phylogenomic databases

eggNOGiNOG243169.
GeneTreeiENSGT00390000011336.
HOGENOMiHOG000188277.
InParanoidiP04840.
KOiK15040.
OMAiKSTHEGA.
OrthoDBiEOG7GXPNQ.

Enzyme and pathway databases

BioCyciYEAST:G3O-33087-MONOMER.
ReactomeiREACT_334110. Mitochondrial protein import.

Miscellaneous databases

NextBioi979947.

Gene expression databases

GenevestigatoriP04840.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
[Graphical view]
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure."
    Mihara K., Sato R.
    EMBO J. 4:769-774(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Molecular genetics of the VDAC ion channel: structural model and sequence analysis."
    Forte M.A., Guy H.R., Mannella C.A.
    J. Bioenerg. Biomembr. 19:341-350(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Bergez P., Doignon F., Crouzet M.
    Yeast 11:967-974(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / FY1676.
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Bienvenut W.V., Peters C.
    Submitted (APR-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-40; 45-108; 125-132; 165-174; 206-234 AND 237-274, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
  7. "Probing the structure of the mitochondrial channel, VDAC, by site-directed mutagenesis: a progress report."
    Blachly-Dyson E., Peng S.Z., Colombini M., Forte M.A.
    J. Bioenerg. Biomembr. 21:471-483(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF LYS-61.
  8. "Probing for the voltage sensor of the VDAC ion channel by site-directed mutagenesis."
    Thomas L., Blachly-Dyson E., Colombini M., Forte M.A.
    Biophys. J. 59:215A-215A(1990)
    Cited for: MUTAGENESIS OF LYS-19 AND ASP-51.
  9. "The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane."
    Lee A.C., Xu X., Blachly-Dyson E., Forte M.A., Colombini M.
    J. Membr. Biol. 161:173-181(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
    Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
    Mol. Cell. Proteomics 6:1896-1906(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-117, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria."
    Hoppins S., Collins S.R., Cassidy-Stone A., Hummel E., Devay R.M., Lackner L.L., Westermann B., Schuldiner M., Weissman J.S., Nunnari J.
    J. Cell Biol. 195:323-340(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AIM5; FCJ1 AND MOS1.
  14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVDAC1_YEAST
AccessioniPrimary (citable) accession number: P04840
Secondary accession number(s): D6W1C3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 12, 1987
Last sequence update: January 22, 2007
Last modified: March 31, 2015
This is version 140 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.