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P04840

- VDAC1_YEAST

UniProt

P04840 - VDAC1_YEAST

Protein

Mitochondrial outer membrane protein porin 1

Gene

POR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Forms a channel through the cell membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Is the major permeability fator of the mitochondrial outer membrane.1 Publication

    GO - Molecular functioni

    1. porin activity Source: UniProtKB-KW
    2. voltage-gated anion channel activity Source: SGD

    GO - Biological processi

    1. apoptotic process Source: SGD
    2. cell redox homeostasis Source: SGD
    3. DNA transport Source: SGD
    4. ion transport Source: SGD
    5. mitochondrion organization Source: SGD

    Keywords - Molecular functioni

    Porin

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33087-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi1.B.8.1.1. the mitochondrial and plastid porin (mpp) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial outer membrane protein porin 1
    Alternative name(s):
    Voltage-dependent anion-selective channel protein 1
    Short name:
    VDAC-1
    Gene namesi
    Name:POR1
    Synonyms:OMP2, VDAC1
    Ordered Locus Names:YNL055C
    ORF Names:N2441, YNL2441C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    SGDiS000005000. POR1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: SGD
    2. mitochondrial intermembrane space Source: Reactome
    3. mitochondrial outer membrane Source: SGD
    4. mitochondrion Source: SGD
    5. pore complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi19 – 191K → E: 2.5-fold reduction in steepness of voltage dependence. 1 Publication
    Mutagenesisi51 – 511D → K: 2-fold increase in steepness of voltage dependence. 1 Publication
    Mutagenesisi61 – 611K → E: Alters the selectivity of VDAC. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 283282Mitochondrial outer membrane protein porin 1PRO_0000050524Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei109 – 1091Phosphoserine2 Publications
    Modified residuei117 – 1171Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP04840.
    PaxDbiP04840.
    PeptideAtlasiP04840.

    2D gel databases

    UCD-2DPAGEP04840.

    Expressioni

    Gene expression databases

    GenevestigatoriP04840.

    Interactioni

    Subunit structurei

    Interacts with AIM5, FCJ1 and MOS1.1 Publication

    Protein-protein interaction databases

    BioGridi35766. 190 interactions.
    DIPiDIP-6453N.
    IntActiP04840. 68 interactions.
    MINTiMINT-677066.
    STRINGi4932.YNL055C.

    Structurei

    3D structure databases

    ProteinModelPortaliP04840.
    SMRiP04840. Positions 3-283.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists mainly of membrane-spanning sided beta-sheets. 19 strands distributed along the entire VDAC protein, have the potential to adopt transmembrane beta pleated sheet structures, which rolled together may form a "beta-barrel" type structure, possessing pore dimensions.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane beta strand

    Phylogenomic databases

    eggNOGiNOG243169.
    GeneTreeiENSGT00390000011336.
    HOGENOMiHOG000188277.
    KOiK15040.
    OMAiYGLMFTE.
    OrthoDBiEOG7GXPNQ.

    Family and domain databases

    Gene3Di2.40.160.10. 1 hit.
    InterProiIPR023614. Porin_dom.
    IPR001925. Porin_Euk.
    IPR027246. Porin_Euk/Tom40.
    [Graphical view]
    PfamiPF01459. Porin_3. 1 hit.
    [Graphical view]
    PRINTSiPR00185. EUKARYTPORIN.
    PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P04840-1 [UniParc]FASTAAdd to Basket

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    MSPPVYSDIS RNINDLLNKD FYHATPAAFD VQTTTANGIK FSLKAKQPVK    50
    DGPLSTNVEA KLNDKQTGLG LTQGWSNTNN LQTKLEFANL TPGLKNELIT 100
    SLTPGVAKSA VLNTTFTQPF FTARGAFDLC LKSPTFVGDL TMAHEGIVGG 150
    AEFGYDISAG SISRYAMALS YFAKDYSLGA TLNNEQITTV DFFQNVNAFL 200
    QVGAKATMNC KLPNSNVNIE FATRYLPDAS SQVKAKVSDS GIVTLAYKQL 250
    LRPGVTLGVG SSFDALKLSE PVHKLGWSLS FDA 283
    Length:283
    Mass (Da):30,428
    Last modified:January 23, 2007 - v4
    Checksum:iC99D03711AF9B54D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti118 – 1181Q → E(PubMed:2408884)Curated
    Sequence conflicti118 – 1181Q → E(PubMed:2442148)Curated
    Sequence conflicti203 – 2075GAKAT → AKATM in AAA35208. (PubMed:2442148)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02324 mRNA. Translation: CAA26184.1.
    M34907 mRNA. Translation: AAA35208.1.
    U12141 Genomic DNA. Translation: AAA99656.1.
    Z71331 Genomic DNA. Translation: CAA95926.1.
    BK006947 Genomic DNA. Translation: DAA10489.1.
    PIRiS58721. MMBYP.
    RefSeqiNP_014343.1. NM_001182894.1.

    Genome annotation databases

    EnsemblFungiiYNL055C; YNL055C; YNL055C.
    GeneIDi855669.
    KEGGisce:YNL055C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X02324 mRNA. Translation: CAA26184.1 .
    M34907 mRNA. Translation: AAA35208.1 .
    U12141 Genomic DNA. Translation: AAA99656.1 .
    Z71331 Genomic DNA. Translation: CAA95926.1 .
    BK006947 Genomic DNA. Translation: DAA10489.1 .
    PIRi S58721. MMBYP.
    RefSeqi NP_014343.1. NM_001182894.1.

    3D structure databases

    ProteinModelPortali P04840.
    SMRi P04840. Positions 3-283.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35766. 190 interactions.
    DIPi DIP-6453N.
    IntActi P04840. 68 interactions.
    MINTi MINT-677066.
    STRINGi 4932.YNL055C.

    Protein family/group databases

    TCDBi 1.B.8.1.1. the mitochondrial and plastid porin (mpp) family.

    2D gel databases

    UCD-2DPAGE P04840.

    Proteomic databases

    MaxQBi P04840.
    PaxDbi P04840.
    PeptideAtlasi P04840.

    Protocols and materials databases

    DNASUi 855669.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL055C ; YNL055C ; YNL055C .
    GeneIDi 855669.
    KEGGi sce:YNL055C.

    Organism-specific databases

    SGDi S000005000. POR1.

    Phylogenomic databases

    eggNOGi NOG243169.
    GeneTreei ENSGT00390000011336.
    HOGENOMi HOG000188277.
    KOi K15040.
    OMAi YGLMFTE.
    OrthoDBi EOG7GXPNQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33087-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 979947.

    Gene expression databases

    Genevestigatori P04840.

    Family and domain databases

    Gene3Di 2.40.160.10. 1 hit.
    InterProi IPR023614. Porin_dom.
    IPR001925. Porin_Euk.
    IPR027246. Porin_Euk/Tom40.
    [Graphical view ]
    Pfami PF01459. Porin_3. 1 hit.
    [Graphical view ]
    PRINTSi PR00185. EUKARYTPORIN.
    PROSITEi PS00558. EUKARYOTIC_PORIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and sequencing of cDNA for yeast porin, an outer mitochondrial membrane protein: a search for targeting signal in the primary structure."
      Mihara K., Sato R.
      EMBO J. 4:769-774(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Molecular genetics of the VDAC ion channel: structural model and sequence analysis."
      Forte M.A., Guy H.R., Mannella C.A.
      J. Bioenerg. Biomembr. 19:341-350(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The sequence of a 44 420 bp fragment located on the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Bergez P., Doignon F., Crouzet M.
      Yeast 11:967-974(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: S288c / FY1676.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Bienvenut W.V., Peters C.
      Submitted (MAY-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-40; 45-108; 125-132; 165-174; 206-234 AND 237-274, CLEAVAGE OF INITIATOR METHIONINE, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Probing the structure of the mitochondrial channel, VDAC, by site-directed mutagenesis: a progress report."
      Blachly-Dyson E., Peng S.Z., Colombini M., Forte M.A.
      J. Bioenerg. Biomembr. 21:471-483(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LYS-61.
    8. "Probing for the voltage sensor of the VDAC ion channel by site-directed mutagenesis."
      Thomas L., Blachly-Dyson E., Colombini M., Forte M.A.
      Biophys. J. 59:215A-215A(1991)
      Cited for: MUTAGENESIS OF LYS-19 AND ASP-51.
    9. "The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane."
      Lee A.C., Xu X., Blachly-Dyson E., Forte M.A., Colombini M.
      J. Membr. Biol. 161:173-181(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Profiling phosphoproteins of yeast mitochondria reveals a role of phosphorylation in assembly of the ATP synthase."
      Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B., van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.
      Mol. Cell. Proteomics 6:1896-1906(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-117, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-109, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria."
      Hoppins S., Collins S.R., Cassidy-Stone A., Hummel E., Devay R.M., Lackner L.L., Westermann B., Schuldiner M., Weissman J.S., Nunnari J.
      J. Cell Biol. 195:323-340(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AIM5; FCJ1 AND MOS1.
    14. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVDAC1_YEAST
    AccessioniPrimary (citable) accession number: P04840
    Secondary accession number(s): D6W1C3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 136 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3