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Protein

Carboxypeptidase E

Gene

CPE

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing.

Catalytic activityi

Release of C-terminal arginine or lysine residues from polypeptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Zinc; catalyticBy similarity1
Metal bindingi116Zinc; catalyticBy similarity1
Metal bindingi247Zinc; catalyticBy similarity1
Active sitei341Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-264876. Insulin processing.

Protein family/group databases

MEROPSiM14.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase E (EC:3.4.17.10)
Short name:
CPE
Alternative name(s):
Carboxypeptidase H
Short name:
CPH
Enkephalin convertase
Prohormone-processing carboxypeptidase
Gene namesi
Name:CPE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 17

Subcellular locationi

  • Cytoplasmic vesiclesecretory vesicle membrane By similarity; Peripheral membrane protein By similarity
  • Secreted By similarity

  • Note: Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000030838128 – 41Activation peptide1 PublicationAdd BLAST14
ChainiPRO_000021278542 – 475Carboxypeptidase EAdd BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi138N-linked (GlcNAc...)Sequence analysis1
Glycosylationi389N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP04836.
PRIDEiP04836.

Expressioni

Gene expression databases

BgeeiENSBTAG00000016514.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021955.

Structurei

3D structure databases

ProteinModelPortaliP04836.
SMRiP04836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiP04836.
KOiK01294.
OMAiKNYWEDN.
OrthoDBiEOG091G06A9.
TreeFamiTF315592.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRGGCALL VLCGSLAACA WLLGAEARGP GGPVAGARRR RRPQEDGISF
60 70 80 90 100
EYHRYPELRE ALVSVWLQCA AVSRIYTVGR SFEGRELLVL ELSDNPGVHE
110 120 130 140 150
PGEPEFKYIG NMHGNEAVGR ELLIFLAQYL CNEYQKGNET IVQLIHNTRI
160 170 180 190 200
HIMPSLNPDG FEKAASQLGE LKDWFVGRSN AQGIDLNRNF PDLDRIVYIN
210 220 230 240 250
EKEGGPNNHL LKNLKKIVDQ NTKLAPETKA VIHWIMDIPF VLSANLHGGD
260 270 280 290 300
LVANYPYDET RSGSAHEYSS CPDDDIFQSL ARAYSSFNPP MSDPDRPPCR
310 320 330 340 350
KNDDDSSFVE GTTNGAAWYS VPGGMQDFNY LSSNCFEITV ELSCEKFPPE
360 370 380 390 400
ETLKNYWEDN KNSLISYIQQ IHRGVKGFVR DLQGNPIANA TLSVEGIDHD
410 420 430 440 450
VTSAKDGDYW RLLVPGNYKL TASAPGYLAI AKKVAVPYSP AVRVDFELES
460 470
FSERKEEEKE ELMEWWKMMS ETLNF
Length:475
Mass (Da):53,309
Last modified:October 23, 2007 - v2
Checksum:i6516FE48E39321BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51E → G AA sequence (PubMed:2396993).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti255Y → H.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142181 mRNA. Translation: AAI42182.1.
X04411 mRNA. Translation: CAA27999.1.
AH012367 Genomic DNA. Translation: AAO03557.1.
AH014826 Genomic DNA. Translation: AAX84651.1.
PIRiA24327.
RefSeqiNP_776328.2. NM_173903.4.
UniGeneiBt.73670.

Genome annotation databases

EnsembliENSBTAT00000021955; ENSBTAP00000021955; ENSBTAG00000016514.
GeneIDi280753.
KEGGibta:280753.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142181 mRNA. Translation: AAI42182.1.
X04411 mRNA. Translation: CAA27999.1.
AH012367 Genomic DNA. Translation: AAO03557.1.
AH014826 Genomic DNA. Translation: AAX84651.1.
PIRiA24327.
RefSeqiNP_776328.2. NM_173903.4.
UniGeneiBt.73670.

3D structure databases

ProteinModelPortaliP04836.
SMRiP04836.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021955.

Protein family/group databases

MEROPSiM14.005.

Proteomic databases

PaxDbiP04836.
PRIDEiP04836.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000021955; ENSBTAP00000021955; ENSBTAG00000016514.
GeneIDi280753.
KEGGibta:280753.

Organism-specific databases

CTDi1363.

Phylogenomic databases

eggNOGiKOG2649. Eukaryota.
ENOG410XX0H. LUCA.
GeneTreeiENSGT00760000119124.
HOGENOMiHOG000232185.
HOVERGENiHBG003410.
InParanoidiP04836.
KOiK01294.
OMAiKNYWEDN.
OrthoDBiEOG091G06A9.
TreeFamiTF315592.

Enzyme and pathway databases

ReactomeiR-BTA-264876. Insulin processing.

Gene expression databases

BgeeiENSBTAG00000016514.

Family and domain databases

Gene3Di2.60.40.1120. 1 hit.
InterProiIPR008969. CarboxyPept-like_regulatory.
IPR014766. CarboxyPept_regulatory_dom.
IPR000834. Peptidase_M14.
[Graphical view]
PfamiPF00246. Peptidase_M14. 1 hit.
[Graphical view]
PRINTSiPR00765. CRBOXYPTASEA.
SMARTiSM00631. Zn_pept. 1 hit.
[Graphical view]
SUPFAMiSSF49464. SSF49464. 1 hit.
PROSITEiPS00132. CARBOXYPEPT_ZN_1. 1 hit.
PS00133. CARBOXYPEPT_ZN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCBPE_BOVIN
AccessioniPrimary (citable) accession number: P04836
Secondary accession number(s): A5PJN4, Q52S88, Q6YI55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: October 23, 2007
Last modified: November 2, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.