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Protein

Carboxypeptidase E

Gene

CPE

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing.

Catalytic activityi

Release of C-terminal arginine or lysine residues from polypeptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Zinc; catalyticBy similarity1
Metal bindingi116Zinc; catalyticBy similarity1
Metal bindingi247Zinc; catalyticBy similarity1
Active sitei341Proton donor/acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM14.005

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase E (EC:3.4.17.10)
Short name:
CPE
Alternative name(s):
Carboxypeptidase H
Short name:
CPH
Enkephalin convertase
Prohormone-processing carboxypeptidase
Gene namesi
Name:CPE
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 17

Organism-specific databases

VGNCiVGNC:27648 CPE

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000030838128 – 41Activation peptide1 PublicationAdd BLAST14
ChainiPRO_000021278542 – 475Carboxypeptidase EAdd BLAST434

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi138N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi389N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP04836
PRIDEiP04836

Expressioni

Gene expression databases

BgeeiENSBTAG00000016514

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021955

Structurei

3D structure databases

ProteinModelPortaliP04836
SMRiP04836
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2649 Eukaryota
ENOG410XX0H LUCA
GeneTreeiENSGT00760000119124
HOGENOMiHOG000232185
HOVERGENiHBG003410
InParanoidiP04836
KOiK01294
OMAiEELMDWW
OrthoDBiEOG091G06A9
TreeFamiTF315592

Family and domain databases

InterProiView protein in InterPro
IPR008969 CarboxyPept-like_regulatory
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
PRINTSiPR00765 CRBOXYPTASEA
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit
SUPFAMiSSF49464 SSF49464, 1 hit
PROSITEiView protein in PROSITE
PS00132 CARBOXYPEPT_ZN_1, 1 hit
PS00133 CARBOXYPEPT_ZN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P04836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARRGGCALL VLCGSLAACA WLLGAEARGP GGPVAGARRR RRPQEDGISF
60 70 80 90 100
EYHRYPELRE ALVSVWLQCA AVSRIYTVGR SFEGRELLVL ELSDNPGVHE
110 120 130 140 150
PGEPEFKYIG NMHGNEAVGR ELLIFLAQYL CNEYQKGNET IVQLIHNTRI
160 170 180 190 200
HIMPSLNPDG FEKAASQLGE LKDWFVGRSN AQGIDLNRNF PDLDRIVYIN
210 220 230 240 250
EKEGGPNNHL LKNLKKIVDQ NTKLAPETKA VIHWIMDIPF VLSANLHGGD
260 270 280 290 300
LVANYPYDET RSGSAHEYSS CPDDDIFQSL ARAYSSFNPP MSDPDRPPCR
310 320 330 340 350
KNDDDSSFVE GTTNGAAWYS VPGGMQDFNY LSSNCFEITV ELSCEKFPPE
360 370 380 390 400
ETLKNYWEDN KNSLISYIQQ IHRGVKGFVR DLQGNPIANA TLSVEGIDHD
410 420 430 440 450
VTSAKDGDYW RLLVPGNYKL TASAPGYLAI AKKVAVPYSP AVRVDFELES
460 470
FSERKEEEKE ELMEWWKMMS ETLNF
Length:475
Mass (Da):53,309
Last modified:October 23, 2007 - v2
Checksum:i6516FE48E39321BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51E → G AA sequence (PubMed:2396993).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti255Y → H1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC142181 mRNA Translation: AAI42182.1
X04411 mRNA Translation: CAA27999.1
AH012367 Genomic DNA Translation: AAO03557.1
AH014826 Genomic DNA Translation: AAX84651.1
PIRiA24327
RefSeqiNP_776328.2, NM_173903.4
UniGeneiBt.73670

Genome annotation databases

EnsembliENSBTAT00000021955; ENSBTAP00000021955; ENSBTAG00000016514
GeneIDi280753
KEGGibta:280753

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCBPE_BOVIN
AccessioniPrimary (citable) accession number: P04836
Secondary accession number(s): A5PJN4, Q52S88, Q6YI55
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: October 23, 2007
Last modified: March 28, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health